EPO (NM_000799) Human Untagged Clone

CAT#: SC125341

EPO (untagged)-Human erythropoietin (EPO)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EPO
• Synonyms: DBAL; ECYT5; EP; MVCD2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC125341 sequence for NM_000799 edited (data generated by NextGen Sequencing)
  ATGGGGGTGCACGAATGTCCTGCCTGGCTGTGGCTTCTCCTGTCCCTGCTGTCGCTCCCT
  CTGGGCCTCCCAGTCCTGGGCGCCCCACCACGCCTCATCTGTGACAGCCGAGTCCTGGAG
  AGGTACCTCTTGGAGGCCAAGGAGGCCGAGAATATCACGACGGGCTGTGCTGAACACTGC
  AGCTTGAATGAGAATATCACTGTCCCAGACACCAAAGTTAATTTCTATGCCTGGAAGAGG
  ATGGAGGTCGGGCAGCAGGCCGTAGAAGTCTGGCAGGGCCTGGCCCTGCTGTCGGAAGCT
  GTCCTGCGGGGCCAGGCCCTGTTGGTCAACTCTTCCCAGCCGTGGGAGCCCCTGCAGCTG
  CATGTGGATAAAGCCGTCAGTGGCCTTCGCAGCCTCACCACTCTGCTTCGGGCTCTGGGA
  GCCCAGAAGGAAGCCATCTCCCCTCCAGATGCGGCCTCAGCTGCTCCACTCCGAACAATC
  ACTGCTGACACTTTCCGCAAACTCTTCCGAGTCTACTCCAATTTCCTCCGGGGAAAGCTG
  AAGCTGTACACAGGGGAGGCCTGCAGGACAGGGGACAGATGA
  
  Clone variation with respect to NM_000799.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000799 unedited
  GACCTACTATATGGCGGCCGCGAATTCGAGATGGGGGTGCACGAATGTCCTGCCTGGCTG
  TGGCTTCTCCTGTTCCTGCTGTCGCTCCCTCTGGGCCTCCCAGTCCTGGGCGCCCCACCA
  CGCCTCATCTGTGACAGCCGAGTCCTGGAGAGGTACCTCTTGGAGGCCAAGGAGGCCGAG
  AATATCACGACGGGCTGTGCTGAACACTGCAGCTTGAATGAGAATATCACTGTCCCAGAC
  ACCAAAGTTAATTTCTATGCCTGGAAGAGGATGGAGGTCGGGCAGCAGGCCGTAGAAGTC
  TGGCAGGGCCTGGCCCTGCTGTCGGAAGCTGTCCTGCGGGGCCAGGCCCTGTTGGTCAAC
  TCTTCCCAGCCGTGGGAGCCCCTGCAGCTGCATGTGGATAAAGCCGTCAGTGGCCTTCGC
  AGCCTCACCACTCTGCTTCGGGCTCTGGGAGCCCAGAAGGAAGCCATCTCCCCTCCAGAT
  GCGGCCTCAGCTGCTCCACTCCGAACAATCACTGCTGACACTTTCCGCAAACTCTTCCGA
  GTCTACTCCAATTTCCTCCGGGGAAAGCTGAAGCTGTACACAGGGGAGGCCTGCATGACA
  GGGGACAGATGACCAGGCTCGACTCTAGATTGCGGCCGCGGTCATAGCTGTTTCCTGAAC
  AGATCCCCGGTGGGATTCCTGGTGACCCCTCCCCAGTGCCTTTTCGGGCCCTGGAAGTTG
  GCACTCCGTGGCCAACCAACCTGGCCTAATAAAATTAAATGCACCATTTGTCTGACAGGG
  G
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000799 unedited
  CCGTGGCGCATTGGNGATGGCACTTCCCAGGNCCGGNGAAAGCACTGGGGNAGTGGGTCA
  CAGGGCATGCCACCCGGGTTCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAG
  TCGAGCCTGGTCATCTGTCCCCTGTCCTGCAGGCCTCCCCTGTGTACAGCTTCAGCTTTC
  CCCGGAGGAAATTGGAGTAGACTCGGAAGAGTTTGCGGAAAGTGTCAGCAGTGATTGTTC
  GGAGTGGAGCAGCTGAGGCCGCATCTGGAGGGGAGATGGCTTCCTTCTGGGCTCCCAGAG
  CCCGAAGCAGAGTGGTGAGGCTGCGAAGGCCACTGACGGCTTTATCCACATGCAGCTGCA
  GGGGCTCCCACGGCTGGGAAGAGTTGACCAACAGGGCCTGGCCCCGCANGACAGCTTCCG
  ACAGCAGGGCCAGGCCCTGCCAGACTTCTACGGCCTGCTGCCCGACCTCCATCCTCTTCC
  AGGCATAGAAATTAACTTTGGTGTCTGGGACAGTGATATTCTCATTCAAGCTGCAGTGTT
  CAGCACAGCCCGTCGTGATATTCTCGGCCTCCTTGGCCTNCAAGAGGTACCTCTCCANGA
  CTCGGCTGTCACAGATGAGGCGTGGTGGGGCGCCCAGNACTGGGAGGCCCAGAGGGAGCG
  ACAGCAGGGACAAGAGAAGCACAGCCAGGCAGGACATTCGTGCACCCCCATCTCGAATTC
  GCGGCCCGCCTATAGTGAGTCGTATTACAAAATTCTGACGGTTCACTAAACGAGCTCTGC
  TTATTTTAGACCTCCAACCGTCACGCCTACCGGCCATTTTCCGTTCACGGGGCCGGGNTT
  ATTACAACATTTTGGGAAAGTCCCTTTGATTTTGGTGCCCAAAACAAACTCCCCTTGTCC
  TTCAAGGGGGTTGAAAACTA
  
• Restriction Sites: Please inquire
• ACCN: NM_000799
• Insert Size: 640 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000799.2, NP_000790.2
• RefSeq Size: 1340 bp
• RefSeq ORF: 582 bp
• Locus ID: 2056
• Cytogenetics: 7q22.1
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction, Hematopoietic cell lineage, Jak-STAT signaling pathway
• Gene Summary: 'This gene encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The encoded protein is mainly synthesized in the kidney, secreted into the blood plasma, and binds to the erythropoietin receptor to promote red blood cell production, or erythropoiesis, in the bone marrow. Expression of this gene is upregulated under hypoxic conditions, in turn leading to increased erythropoiesis and enhanced oxygen-carrying capacity of the blood. Expression of this gene has also been observed in brain and in the eye, and elevated expression levels have been observed in diabetic retinopathy and ocular hypertension. Recombinant forms of the encoded protein exhibit neuroprotective activity against a variety of potential brain injuries, as well as antiapoptotic functions in several tissue types, and have been used in the treatment of anemia and to enhance the efficacy of cancer therapies. [provided by RefSeq, Aug 2017]'