Osteopontin (SPP1) (NM_000582) Human Untagged Clone

CAT#: SC125352

SPP1 (untagged)-Human secreted phosphoprotein 1 (SPP1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SPP1
• Synonyms: BNSP; BSPI; ETA-1; OPN
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC125352 sequence for NM_000582 edited (data generated by NextGen Sequencing)
  ATGAGAATTGCAGTGATTTGCTTTTGCCTCCTAGGCATCACCTGTGCCATACCAGTTAAA
  CAGGCTGATTCTGGAAGTTCTGAGGAAAAGCAGCTTTACAACAAATACCCAGATGCTGTG
  GCCACATGGCTAAACCCTGACCCATCTCAGAAGCAGAATCTCCTAGCCCCACAGACCCTT
  CCAAGTAAGTCCAACGAAAGCCATGACCACATGGATGATATGGATGATGAAGATGATGAC
  GACCATGTGGACAGCCAGGACTCCATTGACTCGAACGACTCTGATGATGTAGATGACACT
  GATGATTCTCACCAGTCTGATGAGTCTCACCATTCTGATGAATCTGATGAACTGGTCACT
  GATTTTCCCACGGACCTGCCAGCAACCGAAGTTTTCACTCCAGTTGTCCCCACAGTAGAC
  ACATATGATGGCCGAGGTGATAGTGTGGTTTATGGACTGAGGTCAAAATCTAAGAAGTTT
  CGCAGACCTGACATCCAGTACCCTGATGCTACAGACGAGGACATCACCTCACACATGGAA
  AGCGAGGAGTTGAATGGTGCATACAAGGCCATCCCCGTTGCCCAGGACCTGAACGCGCCT
  TCTGATTGGGACAGCCGTGGGAAGGACAGTTATGAAACGAGTCAGCTGGATGACCAGAGT
  GCTGAAACCCACAGCCACAAGCAGTCCAGATTATATAAGCGGAAAGCCAATGATGAGAGC
  AATGAGCATTCCGATGTGATTGATAGTCAGGAACTTTCCAAAGTCAGCCGTGAATTCCAC
  AGCCATGAATTTCACAGCCATGAAGATATGCTGGTTGTAGACCCCAAAAGTAAGGAAGAA
  GATAAACACCTGAAATTTCGTATTTCTCATGAATTAGATAGTGCATCTTCTGAGGTCAAT
  TAA
  
  Clone variation with respect to NM_000582.2
  240 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000582 unedited
  TTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCACAGCATCGTCGGG
  ACCAGACTCGTCTCAGGCCAGTTGCAGCCTTCTCAGCCAAACGCCGACCAAGGAAAACTC
  ACTACCATGAGAATTGCAGTGATTTGCTTTTGCCTCCTAGGCATCACCTGTGCCATACCA
  GTTAAACAGGCTGATTCTGGAAGTTCTGAGGAAAAGCAGCTTTACAACAAATACCCAGAT
  GCTGTGGCCACATGGCTAAACCCTGACCCATCTCAGAAGCAGAATCTCCTAGCCCCACAG
  ACCCTTCCAAGTAAGTCCAACGAAAGCCATGACCACATGGATGATATGGATGATGAAGAT
  GATGATGACCATGTGGACAGCCAGGACTCCATTGACTCGAACGACTCTGATGATGTAGAT
  GACACTGATGATTCTCACCAGTCTGATGAGTCTCACCATTCTGATGAATCTGATGAACTG
  GTCACTGATTTTCCCACGGACCTGCCAGCAACCGAAGTTTTCACTCCAGTTGTCCCCACA
  GTAGACACATATGATGGCCGAGGTGATAGTGTGGTTTATGGACTGAGGTCAAAATCTAAG
  AAGTTTCGCAGACCTGACATCCAGTACCCTGATGCTACAGACGANGACATCACCTCACAC
  ATGGAAAAGCGAGGAGTTGAATGGTGCATACAAGGCCATCCCCGTTGCCCAGACCTNGAC
  GCGCCTTCTGATTGGGACAGCCGTGGGAAGGACAGTTATGAAACGAGTCACCTGAATGAC
  CAGAGTGCTGAAACCCCCAGCCCCAACCAGTCCAGAATATATAACCGGGAAGCCCATGAT
  GAGAGCCATTGACCTTCCCGATGTGATTGATAGTCAGGAAACTTTCCAAAGTCGCCCGGA
  ATTCCCCACCCTGAATCTCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000582 unedited
  GTTGCGGCTCAGGGGATGGTCACTTCCCAGTTCCGGGAAAGCACTGAGGCAGGGGCTCAC
  AGGGCATGCCACCCGGGTTCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGT
  CGAGTTTTTTTTTTTTTTTTTTGCATTCAAGATAAAAGATTTATTCACACCACAAAAAGA
  TAATCACAACAAAATATACACTAACTTAAAAAACAAAAGATTATAGTGTCTTAAAATGTT
  ATATTCTCTTTTTAAGTGGGTAAAAGTATTTTGTTTGCTTCTACATAAATTTCTATTCGT
  GAGAGAATAACAAATATTAAAATACAGTGATAGTTTGCATTTCTTCTATAGAATGAACAT
  AGACATAACCCTGAAGCTTTTAGTTTACAGGGAGTTTCCATGAAGCCACAAACTAAACTA
  ATTATCAAACACATTAGTTATTTCCAGACTCAAATAGATACACATTCAACCAATAAACTG
  AGAAAGAAGCATTTCATGTTCTCTTTCATTTTGCTATAAAGCATTTTTTCTTTTGACTAA
  ATGCAAAGAGAGAAATTGTATTTTTTCTCCTTTTAATTGACCTCAGAAGATGCACTATCT
  AATTCATGAGAAATACGAAATTTCAGGTGTTTATCTTCTTCCTTACTTTTGGGGTCTACA
  ACCAGCATATCTTCATGGCTGTGAAATTCATGGCTGTGGAATTCACGGCTGACTTTGGAA
  AGTTCCTGACTATCAATCACATCGGAATGCTCATTGCTCTCATCATTGGCTTTCCGCTTA
  TATAATCTGGACTGCTTGTGGCTGTGGGTTTCAGCACTCTGGTCATCCAGCTGACTCGTT
  TCATAACTGTCCCTCCCACGGGTGGCCCAATCATAAAGCGCGTTCAGGTTCCTGAGCAAC
  GGGGATGA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000582
• Insert Size: 1460 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000582.2, NP_000573.1
• RefSeq Size: 1616 bp
• RefSeq ORF: 903 bp
• Locus ID: 6696
• Cytogenetics: 4q22.1
• Domains: OSTEO
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: ECM-receptor interaction, Focal adhesion, Toll-like receptor signaling pathway
• Gene Summary: 'The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]'
  Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence and lacks an alternate in-frame exon compared to variant 5. The resulting isoform (OPN-b, as described in PubMed: 7837791) has a shorter and distinct N-terminus and lacks an alternate internal segment compared to isoform e.