ETS1 (NM_005238) Human Untagged Clone

CAT#: SC125359

ETS1 (untagged)-Human v-ets erythroblastosis virus E26 oncogene homolog 1 (avian) (ETS1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ETS1
• Synonyms: c-ets-1; ETS-1; EWSR2; p54
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC125359 sequence for NM_005238 edited (data generated by NextGen Sequencing)
  ATGAAGGCGGCCGTCGATCTCAAGCCGACTCTCACCATCATCAAGACGGAAAAAGTCGAT
  CTGGAGCTTTTCCCCTCCCCGGATATGGAATGTGCAGATGTCCCACTATTAACTCCAAGC
  AGCAAAGAAATGATGTCTCAAGCATTAAAAGCTACTTTCAGTGGTTTCACTAAAGAACAG
  CAACGACTGGGGATCCCAAAAGACCCCCGGCAGTGGACAGAAACCCATGTTCGGGACTGG
  GTGATGTGGGCTGTGAATGAATTCAGCCTGAAAGGTGTAGACTTCCAGAAGTTCTGTATG
  AATGGAGCAGCCCTCTGCGCCCTGGGTAAAGACTGCTTTCTCGAGCTGGCCCCAGACTTT
  GTTGGGGACATCTTATGGGAACATCTAGAGATCCTGCAGAAAGAGGATGTGAAACCATAT
  CAAGTTAATGGAGTCAACCCAGCCTATCCAGAATCCCGCTATACCTCGGATTACTTCATT
  AGCTATGGTATTGAGCATGCCCAGTGTGTTCCACCATCGGAGTTCTCAGAGCCCAGCTTC
  ATCACAGAGTCCTATCAGACGCTCCATCCCATCAGCTCGGAAGAGCTCCTCTCCCTCAAG
  TATGAGAATGACTACCCCTCGGTCATTCTCCGAGACCCTCTCCAGACAGACACCTTGCAG
  AATGACTACTTTGCTATCAAACAAGAAGTCGTCACCCCAGACAACATGTGCATGGGGAGG
  ACCAGTCGTGGTAAACTCGGGGGCCAGGACTCTTTTGAAAGCATAGAGAGCTACGATAGT
  TGTGATCGCCTCACCCAGTCCTGGAGCAGCCAGTCATCTTTCAACAGCCTGCAGCGTGTT
  CCCTCCTATGACAGCTTCGACTCAGAGGACTATCCGGCTGCCCTGCCCAACCACAAGCCC
  AAGGGCACCTTCAAGGACTATGTGCGGGACCGTGCTGACCTCAATAAGGACAAGCCTGTC
  ATTCCTGCTGCTGCCCTAGCTGGCTACACAGGCAGTGGACCAATCCAGCTGTGGCAGTTT
  CTTCTGGAATTACTCACTGATAAATCCTGTCAGTCTTTTATCAGCTGGACAGGAGATGGC
  TGGGAATTCAAACTTTCTGACCCAGATGAGGTGGCCAGGAGATGGGGAAAGAGGAAAAAC
  AAACCTAAGATGAATTATGAGAAACTGAGCCGTGGCCTACGCTACTATTACGACAAAAAC
  ATCATCCACAAGACAGCGGGGAAACGCTACGTGTACCGCTTTGTGTGTGACCTGCAGAGC
  CTGCTGGGGTACACCCCTGAGGAGCTGCACGCCATGCTGGACGTCAAGCCAGATGCCGAC
  GAGTGA
  
  Clone variation with respect to NM_005238.3
  1011 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005238 unedited
  CCGCGAATTCGGCACGAGCGGGAATTTGAGATTTTTGGGAAGAAAGTCGGATTTCCCCCG
  TCCCCTTCCCCCTGTTACTAATCCTCATTAAAAAGAAAAACAACAGTAACTGCAAACTTG
  CTACCATCCCGTACGTCCCCCACTCCTGGCACCATGAAGGCGGCCGTCGATCTCAAGCCG
  ACTCTCACCATCATCAAGACGGAAAAAGTCGATCTGGAGCTTTTCCCCTCCCCGGATATG
  GAATGTGCAGATGTCCCACTATTAACTCCAAGCAGCAAAGAAATGATGTCTCAAGCATTA
  AAAGCTACTTTCAGTGGTTTCACTAAAGAACAGCAACGACTGGGGATCCCAAAAGACCCC
  CGGCAGTGGACAGAAACCCATGTTCGGGACTGGGTGATGTGGGCTGTGAATGAATTCAGC
  CTGAAAGGTGTAGACTTCCAGAAGTTCTGTATGAATGGAGCAGCCCTCTGCGCCCTGGGT
  AAAGACTGCTTTCTCGAGCTGGCCCCAGACTTTGTTGGGGACATCTTATGGGAACATCTA
  GAGATCCTGCAGAAAGAGGATGTGAAACCATATCAAGTTAATGGAGTCAACCCAGCCTAT
  CCAGAATCCCGCTATACCTCGGATTACTTCATTAGCTATGGTATTGAGCATGCCCCAGTG
  TGNTCCACCATCGGANGTCTCAGAGCCCAGCTTCATCACAGAGTCCTATCAGACGCTCCA
  TNCCATCAGCTCGGAAGAGCTCCTCTCCCTCAGTATGAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005238 unedited
  TATGACCGAGGCTGCAATCNAGGATNCNGAAAAATCCTTTTTTTTTTTTTTTTTTTTTGA
  ATGAAATTCTTTGGTTTTAATTTCACACAGGTTAAAATACAATATGAAATCAGGCTACAG
  TATATAAAAAACTCTCCAGCAAAATGATGTGCCAGCATCAGCTACTAAAATAAACAAACA
  AAAAACTCCGCCATAAGAATTTTTTTGCATGGGGTGGATAAAAAACATCGACACTTACAT
  CGCTACATCTCTAAGCTACCTCAGTTCTGATTTTTAAAAAGCACCTGCTTTTCCTTTTTT
  TCATCTTACTTCTAAATTTTCAGCTTTTAAAAAATATAAATTATATGAAAATACAAGTTG
  GAAAATAGTCAAACACAATATAACATCTTTTTCATCCCTATACTTCTCAGCTTAAAAAAA
  AAGTATTCTTAAAAAAAAAAGTTCACTAACTGAGGCAGTATTCCTGATAATCCCATTTCA
  ACATATATATTTCATATCTGGATATGCCCCCACCAATTCACGGTCTCTCGACAATTCCCT
  CGACCGTAGGCCCGACGCCTTCCACCGCCCCCGACCCCTCCCCCCGCGGGATGGCACGCT
  GAATACAGAAAGCGGCATCACATCCCCCCTCCGCCACCCCTCTATCGGGCCCCCCGCGCC
  CGCCCCCCCCCACACCCCTCCCACGCCCTCCCGCCGCGGGTGTCCCCCCCCCCCTAACCG
  CCGCAATCTATTCCCCCTCTATCCCCGCTCGCGCCCCTCCCCACGGTCAGCCGATCGAAC
  AACCGGGTGGTCACCACGCGCGCCCGCCGCGCCGCCCTTTGTTCGTCCCCCACCCGTCCC
  GTTCCCCGCCCACCACCTCCCCCCCCGCCACCNGCCTACTATGTGACATTTCTTCCCCGC
  CCCCCCCACACCTGGACCCCCATCTTATTTCGCTTCCCCCGTATCACAAATACAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005238
• Insert Size: 5410 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005238.2, NP_005229.1
• RefSeq Size: 5228 bp
• RefSeq ORF: 1326 bp
• Locus ID: 2113
• Cytogenetics: 11q24.3
• Domains: ETS, SAM_PNT
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Dorso-ventral axis formation, Pathways in cancer, Renal cell carcinoma
• Gene Summary: 'This gene encodes a member of the ETS family of transcription factors, which are defined by the presence of a conserved ETS DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T in target genes. These proteins function either as transcriptional activators or repressors of numerous genes, and are involved in stem cell development, cell senescence and death, and tumorigenesis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011]'
  Transcript Variant: This variant (2) uses an alternate promoter, and differs in the 5' UTR and 5' coding region compared to variant 1. The resulting isoform (2, also known as Ets-1 p51) is shorter with a distinct N-terminus compared to isoform 1.