GATA2 (NM_032638) Human Untagged Clone

CAT#: SC125368

GATA2 (untagged)-Human GATA binding protein 2 (GATA2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GATA2
• Synonyms: DCML; IMD21; MONOMAC; NFE1B
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC125368 sequence for NM_032638 edited (data generated by NextGen Sequencing)
  ATGGAGGTGGCGCCGGAGCAGCCGCGCTGGATGGCGCACCCGGCCGTGCTGAATGCGCAG
  CACCCCGACTCACACCACCCGGGCCTGGCGCACAACTACATGGAACCCGCGCAGCTGCTG
  CCTCCAGACGAGGTGGACGTCTTCTTCAATCACCTCGACTCGCAGGGCAACCCCTACTAT
  GCCAACCCCGCTCACGCGCGGGCGCGCGTCTCCTACAGCCCCGCGCACGCCCGCCTGACC
  GGAGGCCAGATGTGCCGCCCACACTTGTTGCACAGCCCGGGTTTGCCCTGGCTGGACGGG
  GGCAAAGCAGCCCTCTCTGCCGCTGCGGCCCACCACCACAACCCCTGGACCGTGAGCCCC
  TTCTCCAAGACGCCACTGCACCCCTCAGCTGCTGGAGGCCCTGGAGGCCCACTCTCTGTG
  TACCCAGGGGCTGGGGGTGGGAGCGGGGGAGGCAGCGGGAGCTCAGTGGCCTCCCTCACC
  CCTACAGCAACCCACTCTGGCTCCCACCTTTTCGGCTTCCCACCCACGCCACCCAAAGAA
  GTGTCTCCTGACCCTAGCACCACGGGGGCTGCGTCTCCAGCCTCATCTTCCGCGGGGGGT
  AGTGCAGCCCGAGGAGAGGACAAGGACGGCGTCAAGTACCAGGTGTCACTGACGGAGAGC
  ATGAAGATGGAAAGTGGCAGTCCCCTGCGCCCAGGCCTAGCTACTATGGGCACCCAGCCT
  GCTACACACCACCCCATCCCCACCTACCCCTCCTATGTGCCGGCGGCTGCCCACGACTAC
  AGCAGCGGACTCTTCCACCCCGGAGGCTTCCTGGGGGGACCGGCCTCCAGCTTCACCCCT
  AAGCAGCGCAGCAAGGCTCGTTCCTGTTCAGAAGGCCGGGAGTGTGTCAACTGTGGGGCC
  ACAGCCACCCCTCTCTGGCGGCGGGACGGCACCGGCCACTACCTGTGCAATGCCTGTGGC
  CTCTACCACAAGATGAATGGGCAGAACCGACCACTCATCAAGCCCAAGCGAAGACTGTCG
  GCCGCCAGAAGAGCCGGCACCTGTTGTGCAAATTGTCAGACGACAACCACCACCTTATGG
  CGCCGAAACGCCAACGGGGACCCTGTCTGCAACGCCTGTGGCCTCTACTACAAGCTGCAC
  AATGTTAACAGGCCACTGACCATGAAGAAGGAAGGGATCCAGACTCGGAACCGGAAGATG
  TCCAACAAGTCCAAGAAGAGCAAGAAAGGGGCGGAGTGCTTCGAGGAGCTGTCAAAGTGC
  ATGCAGGAGAAGTCATCCCCCTTCAGTGCAGCTGCCCTGGCTGGACACATGGCACCTGTG
  GGCCACCTCCCGCCCTTCAGCCACTCCGGACACATCCTGCCCACTCCGACGCCCATCCAC
  CCCTCCTCCAGCCTCTCCTTCGGCCACCCCCACCCGTCCAGCATGGTGACCGCCATGGGC
  TAG
  
  Clone variation with respect to NM_032638.4
  15 c=>g;490 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_032638 unedited
  CCCCCCTATATCCCCCGCCCGTTGACGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCT
  ATATAAGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAG
  CGGCCGCGAATTCGGCACGAGGGGCAGCTGGCGCCAGGGCGGCCGGAGGATGCCGAGGGG
  CCGGAGCCGGGCGGGCCCGAGGCCGAGGCGCACTCTACCCCCAGCTCCTACCCTGTAAGC
  CCCGCCAGCCTCCGGACGTGCTGTCCCTGGGCCCGTCGCCCTCGGGGCTCCCGCCGGAAC
  TCCTTCACTCTCAGAGGCCGAGTCCCTCCCCTCCCCACGGCTGCGTGTGGCCGTTGCCGT
  CTGCACCCAGACCCTGAGCCGCCGCCGCCGGCCATGGAGGTGGCGCCGGAGCAGCCGCGC
  TGGATGGCGCACCCGGCCGTGCTGAATGCGCAGCACCCCGACTCACACCACCCGGGCCTG
  GCGCACAACTACATGGAACCCGCGCAGCTGCTGCCTCCAGACGAGGTGGACGTCTTCTTC
  AATCACCTCGACTCGCAGGGCAACCCCTACTATGCCAACCCCGCTCACGCGCGGGCGCGC
  GTCTCCTACAGCCCCGCGCACGCCCGCCTGACCGGAGGCCAGATGTGCCGCCCACACTTG
  TTGCACAGCCCGGGTTTGCCCTGGCTGAACGGNGGCAAAGCAGCCCTCTCTGCCGCTGCG
  GCCCACCACCACAACCCCTGGACCGTGAGCCCCTTCTCCAAGACGCCACTGCACCCCTCA
  GCTGCTGGAGGCCCTGGAGGCCCACTCTCTGTGTACTCAGGGCTGGGGGGTGGGAGCGGN
  GGAGGCACCGGGAGCTCAGTGGCCTCCCTCACCTTAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_032638 unedited
  CATGGAATGGCACTTCCAGGGCCAGNAAAGCACTGGGGNAGGGTCACAGGATGCCACCCG
  GGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTT
  TTTTTTTTTTTCACAAAAAGTATTTTATTATTCTTAACAGTACTCACTTTAAAGGAATAA
  GAGGATAGCATACATTTTTTACAGACAATATATAAATGTTGTACATAATTAACAATAACT
  TAGTTCACTAATCCAAAATAAAACAAGCCAAATAAAACATAAAAACAGAAAATACTGCCG
  ATTCTTTTTCTTATGCGGACACTAGTACAAAATAAGTTACTTCTGGCCGTGGTGCTCCCT
  GCAGCGACTGCCCGCCCATATTGCACTTGGTCACTACATCAGCACAATCCTCCTCCTGGG
  CCAGGGGCCCCTCACAGGCCACCACACCCCGCCGTCACCGCATACAGAATCTAAGCTCGG
  GACACGTTTATATACAGCTGTACCTTGGGAAGGATCCAGACAGCCACAGTAAAGCTGGAC
  CTGAGACCCCAGCAGGGACACAGCCTCTCCCTGGGTCCTGGTCCTGACCTGCCCCCAACT
  CCTGCCTTCACCCCCTGGCAGGCCAGCAAATGCCAACCAGCCCCCAGAAACCAGGGCAGC
  AAAGGCCCCTCCCCACCCCCAGCTTTCATACTAGGGCTGTGGGATCCCAGCTCTTTTCCA
  AAAAGATTTGCAAGCTCNCACCTGTGTGTAGGTTTTATTCCTTTCATAGCAGGGCTCCCT
  GTGCTACGTACATCAACTGGACCCAAAAGAGTTAAAAATAAACATTTACTCATCNCGAGT
  AAGCCCTAGCATTTTTTTAAAATTTTCTTTTGCCCAAAATAGAGCCAAGGGTTGAAGGTT
  AACAAAAAAGATGTTTTCAGGGTCCACCGAAAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_032638
• Insert Size: 3700 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032638.3, NP_116027.2
• RefSeq Size: 3383 bp
• RefSeq ORF: 1443 bp
• Locus ID: 2624
• Cytogenetics: 3q21.3
• Domains: GATA
• Protein Families: Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors
• Gene Summary: 'This gene encodes a member of the GATA family of zinc-finger transcription factors that are named for the consensus nucleotide sequence they bind in the promoter regions of target genes. The encoded protein plays an essential role in regulating transcription of genes involved in the development and proliferation of hematopoietic and endocrine cell lineages. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Mar 2009]'
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same isoform (1).