ID1 (NM_002165) Human Untagged Clone

CAT#: SC125462

ID1 (untagged)-Human inhibitor of DNA binding 1, dominant negative helix-loop-helix protein (ID1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ID1
• Synonyms: bHLHb24; ID
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_002165, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAAAGTCGCCAGTGGCAGCACCGCCACCGCCGCCGCGGGCCCCAGCTGCGCGCTGAAGGCCGGCAAGA
  CAGCGAGCGGTGCGGGCGAGGTGGTGCGCTGTCTGTCTGAGCAGAGCGTGGCCATCTCGCGCTGCGCCGG
  GGGCGCCGGGGCGCGCCTGCCTGCCCTGCTGGACGAGCAGCAGGTAAACGTGCTGCTCTACGACATGAAC
  GGCTGTTACTCACGCCTCAAGGAGCTGGTGCCCACCCTGCCCCAGAACCGCAAGGTGAGCAAGGTGGAGA
  TTCTCCAGCACGTCATCGACTACATCAGGGACCTTCAGTTGGAGCTGAACTCGGAATCCGAAGTTGGAAC
  CCCCGGGGGCCGAGGGCTGCCGGTCCGGGCTCCGCTCAGCACCCTCAACGGCGAGATCAGCGCCCTGACG
  GCCGAGGCGGCATGCGTTCCTGCGGACGATCGCATCTTGTGTCGCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002165 unedited
  AATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGTTTCCGTATCTGCTTCGGG
  CTTCCACCTCATTTTTTTCGCTTTGCCCATTCTGTTTCAGCCAGTCGCCAAGAATCATGA
  AAGTCGCCAGTGGCAGCACCGCCACCGCCGCCGCGGGCCCCAGCTGCGCGCTGAAGGCCG
  GCAAGACAGCGAGCGGTGCGGGCGAGGTGGTGCGCTGTCTGTCTGAGCAGAGCGTGGCCA
  TCTCGCGCTGCGCCGGGGGCGCCGGGGCGCGCCTGCCTGCCCTGCTGGACGAGCAGCAGG
  TAAACGTGCTGCTCTACGACATGAACGGCTGTTACTCACGCCTCAAGGAGCTGGTGCCCA
  CCCTGCCCCAGAACCGCAAGGTGAGCAAGGTGGAGATTCTCCAGCACGTCATCGACTACA
  TCAGGGACCTTCAGTTGGAGCTGAACTCGGAATCCGAAGTTGGGACCCCCGGGGGCCGAG
  GGCTGCCGGTCCGGGCTCCGCTCAGCACCCTCAACGGCGAGATCAGCGCCCTGACGGCCG
  AGGCGGCATGCGTTCCTGCGGACGATCGCATCTTGTGTCGCTGAAGCGCCTCCCCCAGGG
  ACCGGCGGACCCCAGCCATCCAGGGGGCAAGAGGAATTACGTGCTCTGTGGGTCTCCCCC
  AACGCGCCTCGCCGGATCTGAGGGAGAACAAGACCGATCGGCGGCCACTGCGCCCTTAAC
  TGCATCCAGCCTGGGGCTGAGGCTGANGCACTGGCGAGGAGAGGGCGCTCCTCTCTGCAC
  ACCTACTAGTCACCAGAGACTTTGGGNGGTGGGATTTCACTCGTGTGTTTTCTATTTTTG
  AAAAGCAGACATTTTAAAAATGCTCACGTTTGGTGGCTCTCANATTCTGAGAAANTGCTT
  TGTATGTATATACATGATCACGACTGAGATATGTTTTACATAGTCTGTGGGCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002165 unedited
  CCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTAACCATCTATTTTATTTG
  TTTAATAACAAAAAAACAGCCCCACAAAACTATTGTAAAACAATATTCTCAGTCGGGGAT
  CATTGTAATATACAATACAAAGCAATTTCCTCAAAAATCTGAGAAGCACCAAACGTGACC
  ATTTTTTAAAATGTCTGCTTTTCAAAAAATAAAAACACACGAGTGGAATCCCACCCCCTA
  AAGTCTCTGGTGACTAGTAGGGGTGCAAAGAGGAGCGCCCTCTCCTCGCCAGTGCCTCAG
  CCTCAGCCCCAGGCTGGATGCAGTTAAGGGCGCAGTGGCCGCCGATCGGTCTTGTTCTCC
  CTCAAATCCGGCGAGGCGCGTTGGGGGAGACCCACAGAGCACGTAATTCCTCTTGCCCCC
  TGGATGGCTGGGGTCCGCCGGTCCCTGGGGGAGGCGCTTCAGCGACACAAGATGCGATCG
  TCCGCAGGAACGCATGCCGCCTCGGCCGTCAGGGCGCTGATCTCGCCGTTGAGGGTGCTG
  AGCGGAGCCCGGACCGGCAGCCCTCGGCCCCCGGGGGTCCCAACTTCGGATTCCGAGTTC
  AGCTCCAACTGAAGGTCCCTGATGTACTCGATGACGTGCTGGAGAATCTCCACCTTGCTC
  ACCTTGCCGTTCTGGGGCAGGGTGGGCACCAGCTCCTTGAGGCGTTGAGAACAGCCGTTC
  ATGTCGTAAAGCAGCACGTTTACCTGCTGCTCGCCCACAGGAAGGCCAGGCCCCCCCCGC
  GCCCCCGCGCACCGCGAAATGCCCCCTCTCTGCTACACGTAAGTGCACCACCTCCCCCGA
  ACGTTCCGTGTTTGGCGGCCTTCACGCCCAATTGGGGCCCGCGGGGCGGGGCGGGCTGCT
  ACTGCCACTTAATGATTCTGGCCTTGGCCGAACCAATGGCCACCCAAAAAGTAGGGAACC
  CCGAGCAAATGGAACCTCGGCCGATTGCGCCCTCCTATTGAGTTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002165
• Insert Size: 1060 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002165.2, NP_002156.2
• RefSeq Size: 993 bp
• RefSeq ORF: 468 bp
• Locus ID: 3397
• Cytogenetics: 20q11.21
• Domains: HLH
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: TGF-beta signaling pathway
• Gene Summary: 'The protein encoded by this gene is a helix-loop-helix (HLH) protein that can form heterodimers with members of the basic HLH family of transcription factors. The encoded protein has no DNA binding activity and therefore can inhibit the DNA binding and transcriptional activation ability of basic HLH proteins with which it interacts. This protein may play a role in cell growth, senescence, and differentiation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) encodes the longer isoform (a, also called ID-1H).