ID1 (NM_002165) Human Untagged Clone

CAT#: SC125462

ID1 (untagged)-Human inhibitor of DNA binding 1, dominant negative helix-loop-helix protein (ID1), transcript variant 1


  "NM_002165" in other vectors (6)

Reconstitution Protocol

USD 540.00

In Stock*

Size
    • 10 ug

Product Images

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol ID1
Synonyms bHLHb24; ID
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_002165, the custom clone sequence may differ by one or more nucleotides


ATGAAAGTCGCCAGTGGCAGCACCGCCACCGCCGCCGCGGGCCCCAGCTGCGCGCTGAAGGCCGGCAAGA
CAGCGAGCGGTGCGGGCGAGGTGGTGCGCTGTCTGTCTGAGCAGAGCGTGGCCATCTCGCGCTGCGCCGG
GGGCGCCGGGGCGCGCCTGCCTGCCCTGCTGGACGAGCAGCAGGTAAACGTGCTGCTCTACGACATGAAC
GGCTGTTACTCACGCCTCAAGGAGCTGGTGCCCACCCTGCCCCAGAACCGCAAGGTGAGCAAGGTGGAGA
TTCTCCAGCACGTCATCGACTACATCAGGGACCTTCAGTTGGAGCTGAACTCGGAATCCGAAGTTGGAAC
CCCCGGGGGCCGAGGGCTGCCGGTCCGGGCTCCGCTCAGCACCCTCAACGGCGAGATCAGCGCCCTGACG
GCCGAGGCGGCATGCGTTCCTGCGGACGATCGCATCTTGTGTCGCTGA


>OriGene 5' read for NM_002165 unedited
AATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGTTTCCGTATCTGCTTCGGG
CTTCCACCTCATTTTTTTCGCTTTGCCCATTCTGTTTCAGCCAGTCGCCAAGAATCATGA
AAGTCGCCAGTGGCAGCACCGCCACCGCCGCCGCGGGCCCCAGCTGCGCGCTGAAGGCCG
GCAAGACAGCGAGCGGTGCGGGCGAGGTGGTGCGCTGTCTGTCTGAGCAGAGCGTGGCCA
TCTCGCGCTGCGCCGGGGGCGCCGGGGCGCGCCTGCCTGCCCTGCTGGACGAGCAGCAGG
TAAACGTGCTGCTCTACGACATGAACGGCTGTTACTCACGCCTCAAGGAGCTGGTGCCCA
CCCTGCCCCAGAACCGCAAGGTGAGCAAGGTGGAGATTCTCCAGCACGTCATCGACTACA
TCAGGGACCTTCAGTTGGAGCTGAACTCGGAATCCGAAGTTGGGACCCCCGGGGGCCGAG
GGCTGCCGGTCCGGGCTCCGCTCAGCACCCTCAACGGCGAGATCAGCGCCCTGACGGCCG
AGGCGGCATGCGTTCCTGCGGACGATCGCATCTTGTGTCGCTGAAGCGCCTCCCCCAGGG
ACCGGCGGACCCCAGCCATCCAGGGGGCAAGAGGAATTACGTGCTCTGTGGGTCTCCCCC
AACGCGCCTCGCCGGATCTGAGGGAGAACAAGACCGATCGGCGGCCACTGCGCCCTTAAC
TGCATCCAGCCTGGGGCTGAGGCTGANGCACTGGCGAGGAGAGGGCGCTCCTCTCTGCAC
ACCTACTAGTCACCAGAGACTTTGGGNGGTGGGATTTCACTCGTGTGTTTTCTATTTTTG
AAAAGCAGACATTTTAAAAATGCTCACGTTTGGTGGCTCTCANATTCTGAGAAANTGCTT
TGTATGTATATACATGATCACGACTGAGATATGTTTTACATAGTCTGTGGGCA
>OriGene 3' read for NM_002165 unedited
CCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTAACCATCTATTTTATTTG
TTTAATAACAAAAAAACAGCCCCACAAAACTATTGTAAAACAATATTCTCAGTCGGGGAT
CATTGTAATATACAATACAAAGCAATTTCCTCAAAAATCTGAGAAGCACCAAACGTGACC
ATTTTTTAAAATGTCTGCTTTTCAAAAAATAAAAACACACGAGTGGAATCCCACCCCCTA
AAGTCTCTGGTGACTAGTAGGGGTGCAAAGAGGAGCGCCCTCTCCTCGCCAGTGCCTCAG
CCTCAGCCCCAGGCTGGATGCAGTTAAGGGCGCAGTGGCCGCCGATCGGTCTTGTTCTCC
CTCAAATCCGGCGAGGCGCGTTGGGGGAGACCCACAGAGCACGTAATTCCTCTTGCCCCC
TGGATGGCTGGGGTCCGCCGGTCCCTGGGGGAGGCGCTTCAGCGACACAAGATGCGATCG
TCCGCAGGAACGCATGCCGCCTCGGCCGTCAGGGCGCTGATCTCGCCGTTGAGGGTGCTG
AGCGGAGCCCGGACCGGCAGCCCTCGGCCCCCGGGGGTCCCAACTTCGGATTCCGAGTTC
AGCTCCAACTGAAGGTCCCTGATGTACTCGATGACGTGCTGGAGAATCTCCACCTTGCTC
ACCTTGCCGTTCTGGGGCAGGGTGGGCACCAGCTCCTTGAGGCGTTGAGAACAGCCGTTC
ATGTCGTAAAGCAGCACGTTTACCTGCTGCTCGCCCACAGGAAGGCCAGGCCCCCCCCGC
GCCCCCGCGCACCGCGAAATGCCCCCTCTCTGCTACACGTAAGTGCACCACCTCCCCCGA
ACGTTCCGTGTTTGGCGGCCTTCACGCCCAATTGGGGCCCGCGGGGCGGGGCGGGCTGCT
ACTGCCACTTAATGATTCTGGCCTTGGCCGAACCAATGGCCACCCAAAAAGTAGGGAACC
CCGAGCAAATGGAACCTCGGCCGATTGCGCCCTCCTATTGAGTTT
Restriction Sites NotI-NotI     
ACCN NM_002165
Insert Size 1060 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002165.2, NP_002156.2
RefSeq Size 993 bp
RefSeq ORF 468 bp
Locus ID 3397
Cytogenetics 20q11.21
Domains HLH
Protein Families Druggable Genome, Transcription Factors
Protein Pathways TGF-beta signaling pathway
Gene Summary 'The protein encoded by this gene is a helix-loop-helix (HLH) protein that can form heterodimers with members of the basic HLH family of transcription factors. The encoded protein has no DNA binding activity and therefore can inhibit the DNA binding and transcriptional activation ability of basic HLH proteins with which it interacts. This protein may play a role in cell growth, senescence, and differentiation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'
Transcript Variant: This variant (1) encodes the longer isoform (a, also called ID-1H).

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