CYP2F1 (NM_000774) Human Untagged Clone

CAT#: SC125485

CYP2F1 (untagged)-Human cytochrome P450, family 2, subfamily F, polypeptide 1 (CYP2F1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CYP2F1
• Synonyms: C2F1; CYP2F; CYPIIF1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_000774 edited
  TCGCCCTTCTCAGCAGCTGCCTTCACCATGGACAGCATAAGCACAGCCATCTTACTCCTG
  CTCCTGGCTCTCGTCTGTCTGCTCCTGACCCTAAGCTCAAGAGATAAGGGAAAGCTGCCT
  CCGGGACCCAGACCCCTCTCAATCCTGGGAAACCTGCTGCTGCTTTGCTCCCAAGACATG
  CTGACTTCTCTCACTAAGCTGAGCAAGGAGTATGGCTCCATGTACACAGTGCACCTGGGA
  CCCAGGCGGGTGGTGGTCCTCAGCGGGTACCAAGCTGTGAAGGAGGCCCTGGTGGACCAG
  GGAGAGGAGTTTAGTGGCCGCGGTGACTACCCTGCCTTTTTCAACTTTACCAAGGGCAAT
  GGCATCGCCTTCTCCAGTGGGGATCGATGGAAGGTCCTGAGACAGTTCTCTATCCAGATT
  CTACGGAATTTCGGGATGGGGAAGAGAAGCATTGAGGAGCGAATCCTAGAGGAGGGCAGC
  TTCCTGCTGGCGGAGCTGCGGAAAACTGAAGGCGAGCCCTTTGACCCCACGTTTGTGCTG
  AGTCGCTCAGTGTCCAACATTATCTGTTCCGTGCTCTTCGGCAGCCGCTTCGACTATGAT
  GATGAGCGTCTGCTCACCATTATCCGCCTTATCAATGACAACTTCCAAATCATGAGCAGC
  CCCTGGGGCGAGTTGTACGACATCTTCCCGAGCCTCCTGGACTGGGTGCCTGGGCCGCAC
  CAACGCATCTTCCAGAACTTCAAGTGCCTGAGAGACCTCATCGCCCACAGCGTCCATGAC
  CACCAGGCCTCGCTAGACCCCAGATCTCCCCGGGACTTCATCCAGTGCTTCCTCACCAAG
  ATGGCAGAGGAGAAGGAGGACCCACTGAGCCACTTCCACATGGATACCCTGCTGATGACC
  ACACATAACCTGCTCTTTGGCGGCACCAAGACGGTGAGCACCACGCTGCACCACGCCTTC
  CTGGCACTCATGAAGTACCCAAAAGTTCAAGCCCGCGTGCAGGAGGAGATCGACCTCGTG
  GTGGGACGCGCGCGGCTGCCGGCGCTGAAGGACCGCGCGGCCATGCCTTACACAGACGCG
  GTGATCCACGAGGTGCAGCGCTTTGCAGACATCATCCCCATGAACTTGCCGCACCGCGTC
  ACTAGGGACACGGCCTTTCGCGGCTTCCTGATACCCAAGGGCACCGATGTCATCACCCTC
  CTTAACACCGTCCACTACGACCCCAGCCAGTTCCTGACGCCCCAGGAGTTCAACCCCGAG
  CATTTTTTGGATGCCACTCAGTCCTTCAAGAAGAGTCCAGCCTTCATGCCCTTCTCAGCT
  GGGCGCCGTCTGTGCCTGGGAGAGTCGCTGGCGCGCATGGAGCTCTTTCTGTACCTCACC
  GCCATCCTGCAGAGCTTTTCGCTGCAGCCGCTGGGTGCGCCCGAGGACATCGACCTGACC
  CCACTCAGCTCAGGTCTTGGCAATTTGCCGCGGCCTTTCCAGCTGTGCCTGCGCCCGCGC
  TAACGCCCCGGCCCTTCCAGATTCGCCTGTGAGCGATGAGGCCCGCCCATGCGGGTTGCT
  ACGTCCCCTTCTTGGTCCACAGTCTGCCTTCATCCCTCTGGCAGTCACGCTGTCTAAGGG
  CGAATTCTGCAGATATCCATCACACTGGCGGCCGCTCGAGCATGCATCTAG
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000774 unedited
  TTGGGGNTTTTTGGTCCCGGACTCGNTACCACTATACGGCCGCCATGTGCTGNGATTCGC
  CCTTCTCAGCAGCTGCCTTCACCTGGACAGCATAAGCACAGCCATCTTACTCCTGCTCCT
  GGCTCTCGTCTGTCTGCTCCTGACCCTAAGCTCAAGAGATAAGGGAAAGCTGCCTCCGGG
  ACCCAGACCCCTCTCAATCCTGGGAAACCTGCTGCTGCTTTGCTCCCAAGACATGCTGAC
  TTCTCTCACTAAGCTGAGCAAGGAGTATGGCTCCATGTACACAGTGCACCTGGGACCCAG
  GCGGGTGGTGGTCCTCAGCGGGTACCAAGCTGTGAAGGAGGCCCTGGTGGACCAGGGAGA
  GGAGTTTAGTGGCCGCGGTGACTACCCTGCCTTTTTCAACTTTACCAAGGGCAATGGCAT
  CGCCTTCTCCAGTGGGGATCGATGGAAGGTCCTGAGACAGTTCTCTATCCAGATTCTACG
  GAATTTCGGGATGGGGAAGAGAAGCATTGAGGAGCGAATCCTAGAGGAGGGCAGCTTCCT
  GCTGGCGGAGCTGCGGAAAACTGAAGGCGAGCCCTTTGACCCCACGTTTGTGCTGAGTCG
  CTCAGTGTCCAACATTATCTGTTCCGTGCTCTTCGGCAGCCGCTTCGACTATGATGATGA
  GCGTCTGCTCACCATTATCCGCCTTATCAATGACAACTTCCAAATCATGAGCAGCCCCTN
  GGGCGAGTTGTACGACATCTTCCCGAGCCTCCTGGACTGGGTGCCTGGGCCGCACCAACG
  CATCTTCCAGAACTCAAGTGCCTGAGAGACCTCATCGCCCACAGCGTCCATGACCACCNA
  GCCTCGCTAGAN
  
• Restriction Sites: Please inquire
• ACCN: NM_000774
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000774.3, NP_000765.2
• RefSeq Size: 1826 bp
• RefSeq ORF: 1476 bp
• Locus ID: 1572
• Cytogenetics: 19q13.2
• Protein Families: Druggable Genome, P450, Transmembrane
• Protein Pathways: Metabolism of xenobiotics by cytochrome P450
• Gene Summary: 'This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is known to dehydrogenate 3-methylindole, an endogenous toxin derived from the fermentation of tryptophan, as well as xenobiotic substrates such as naphthalene and ethoxycoumarin. This gene is part of a large cluster of cytochrome P450 genes from the CYP2A, CYP2B and CYP2F subfamilies on chromosome 19q. [provided by RefSeq, Jul 2008]'