Cytochrome P450 2B6 (CYP2B6) (NM_000767) Human Untagged Clone

CAT#: SC125490

CYP2B6 (untagged)-Human cytochrome P450, family 2, subfamily B, polypeptide 6 (CYP2B6)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CYP2B6
• Synonyms: CPB6; CYP2B; CYP2B7; CYP2B7P; CYPIIB6; EFVM; IIB1; P450
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_000767 edited
  GGCGGCCGCGAATTCGGCACCAGGCAGGGCAGTCAGACCAGGACCATGGAACTCAGCGTC
  CTCCTCTTCCTTGCACTCCTCACAGGACTCTTGCTACTCCTGGTTCAGTGCCACCCTAAC
  ACCCATGACCGCCTCCCACCAGGGCCCCGCCCTCTGCCCCTTTTGGGAAACCTTCTGCAG
  ATGGATAGAAGAGGCCTACTCAAATCCTTTCTGAGGTTCCGAGAGAAATATGGGGACGTC
  TTCACGGTACACCTGGGACCCAGGCCCGTGGTCATGCTGTGTGGAGTAGAGGCCATACGG
  GAGGCCCTTGTGGACAAGGCTGAGGCCTTCTCTGGCCGGGGAAAAATCGCCATGGTCGAC
  CCATTCTTCCGGGGATATGGTGTGATCTTTGCCAATGGAAACCGCTGGAAGGTGCTTCGG
  CGATTCTCTGTGACCACTATGAGGGACTTCGGGATGGGAAAGCGGAGTGTGGAGGAGCGG
  ATTCAGGAGGAGGCTCAGTGTCTGATAGAGGAGCTTCGGAAATCCAAGGGGGCCCTCATG
  GACCCCACCTTCCTCTTCCAGTCCATTACCGCCAACATCATCTGCTCCATCGTCTTTGGA
  AAACGATTCCACTACCAAGATCAAGAGTTCCTGAAGATGCTGAACTTGTTCTACCAGACT
  TTTTCACTCATCAGCTCTGTATTCGGCCAGCTGTTTGAGCTCTTCTCTGGCTTCTTGAAA
  TACTTTCCTGGGGCACACAGGCAAGTTTACAAAAACCTGCAGGAAATCAATGCTTACATT
  GGCCACAGTGTGGAGAAGCACCGTGAAACCCTGGACCCCAGCGCCCCCAAGGACCTCATC
  GACACCTACCTGCTCCACATGGAAAAAGAGAAATCCAACGCACACAGTGAATTCAGCCAC
  CAGAACCTCAACCTCAACACGCTCTCGCTCTTCTTTGCTGGCACTGAGACCACCAGCACC
  ACTCTCCGCTACGGCTTCCTGCTCATGCTCAAATACCCTCATGTTGCAGAGAGAGTCTAC
  AGGGAGATTGAACAGGTGATTGGCCCACATCGCCCTCCAGAGCTTCATGACCGAGCCAAA
  ATGCCATACACAGAGGCAGTCATCTATGAGATTCAGAGATTTTCCGACCTTCTCCCCATG
  GGTGTGCCCCACATTGTCACCCAACACACCAGCTTCCGAGGGTACATCATCCCCAAGGAC
  ACAGAAGTATTTCTCATCCTGAGCACTGCTCTCCATGACCCACACTACTTTGAAAAACCA
  GACGCCTTCAATCCTGACCACTTTCTGGATGCCAATGGGGCACTGAAAAAGACTGAAGCT
  TTTATCCCCTTCTCCTTAGGGAAGCGGATTTGTCTTGGTGAAGGCATCGCCCGTGCGGAA
  TTGTTCCTCTTCTTCACCACCATCCTCCAGAACTTCTCCATGGCCAGCCCCGTGGCCCCA
  GAAGACATCGATCTGACACCCCAGGAGTGTGGTGTGGGCAAAATACCCCCAACATACCAG
  ATCCGCTTCCTGCCCCGCTGAAGGGGCTGAGGGAAGGGGGTCAAAGGATTCCAGGGTCAT
  TCAGTGTCCCCGCCTCTGTAGACAATGGCTCTGACTCCCCGCAACTTCCTGCCTCTGAGA
  GACCTGCTACAAGCCAGCTTCCTTCCCCTCCATGGCACCAGTTGTCTGAGGTCACATTGC
  AAGTGAGTGCAGGAGTGAGATT
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000767 unedited
  TGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGGCAGGGCAGTCAGACCA
  GGACCATGGAACTCAGCGTCCTCCTCTTCCTTGCACTCCTCACAGGACTCTTGCTACTCC
  TGGTTCAGTGCCACCCTAACACCCATGACCGCCTCCCACCAGGGCCCCGCCCTCTGCCCC
  TTTTGGGAAACCTTCTGCAGATGGATAGAAGAGGCCTACTCAAATCCTTTCTGAGGTTCC
  GAGAGAAATATGGGGACGTCTTCACGGTACACCTGGGACCCAGGCCCGTGGTCATGCTGT
  GTGGAGTAGAGGCCATACGGGAGGCCCTTGTGGACAAGGCTGAGGCCTTCTCTGGCCGGG
  GAAAAATCGCCATGGTCGACCCATTCTTCCGGGGATATGGTGTGATCTTTGCCAATGGAA
  ACCGCTGGAAGGTGCTTCGGCGATTCTCTGTGACCACTATGAGGGACTTCGGGATGGGAA
  AGCGGAGTGTGGAGGAGCGGATTCANGAGGAGGCTCAGTGTCTGATAGAGGAGCTTCGGA
  AATCCAAGGGGGCCCTCATGGACCCCACCTTCCTCTTCCAGTCCATTACCCGCCACATCA
  TCTGCNTCATCGTCTTTGGAAAACGATCCACTACCCAGATCAAGAAGTCCCTGAGATGCT
  GAAACTTGTCTACCCAGACTTTTCACTCATCAGCTCTGTATTCGGCCAGCTGTTTGAGCT
  CTTCTCTGGCTTCTGAAATACTTTCCTGGNGGCACACAGCAGTTTACAAAAACCTGGCAG
  AAAATCATGCTTACATTGGCCCAGTGTGGAGAACCACCGTGAAACCTGGACCCAACGCCC
  CAAGGACTNATNGAACCTACCTGCTCCAATGGAANNAGAGAAATCACGCCACAGTGAATT
  AGNCCCAGAACTCNNACTNAACGCTTTTGTTTTNTTGTTGGACTGAAACACAGNACCATC
  TCGNTACGGTNCTGCTAGCTAATACCTCTGTTGCAAAAGTTACAGAGATTGACAGGGATG
  GCCCATGGCTCAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000767 unedited
  NNAAATACTGTGTACCCGGCCCGCATGCGAGGATCGAGTTAATCTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCAGGCTTTAAAAATGTTTA
  TTTAATTCTCAGCAAATTTGAAGGAACATTTCAGGGAAATCCAAAGCCCCCGTGCAAAAT
  CCCCCAGCTTAGGGAACCTGGGCCAAGGGGGCTTTTTTAATAATTGAACCCGTAGGAGGA
  GGCCCTTGGGAATGAGGATGGGGAAAAACTACCCCAGGGGGGGACTGGGGTTTACAACAG
  GCCCAAACCTAAAAAATAGCTTTCAACATGAAAGCCCCCCGCCCCAAAAAATCCCATTTT
  ATGCCTGTAATATCACCCCTTTGGAACCCTGGGGGGGGGGGATCACCTGGGGCCAGGAGC
  TCACCACCAGCCTGGCCAACATGTCCCTCCTAAAACTCCCAAAAAATTACCCGGGCCTGG
  GAATACATGCCTGTAATCCCAGCTACTTGGGAGCTGAAACTGGAAAATCTCTTGATCTTG
  GAAGGCAAAGGTTGCAGTGAACAAAAATTGCCCCATAGCACTCCAGCCTGGGAAACAAAG
  CAAGGTTATTTTCAAAAAAAAAAAAAAACAAATTTGGTTGATTTTGTTTTGGGAAAATTT
  CAAAATTGGAAAGCCCTAGGGACAAAAACCAATTTGCCGGGCCCCAATTTAAAAAAAAAA
  GGGGGTTGGGGAGGGGCCACCTTTAAAGGTTGGGTTTGGGGGGGGGGAAAAAAATAAAAA
  ATTTTGGGCCGTGGGAAATCCCCTGCCTCCGGGGTGTTAAAACCCCCTAAAAAAACAAAT
  TTTCCCCCCCCCTGTGGGGGGGCCCCCTGTGAAACCCCCTCTCCCCGAGGGTTGGGCGGG
  AAAAAATCTTCTCCCCCCCTTTTGGGGGTTTTCCGGCCACAAAGGGCCCCCCCTC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000767
• Insert Size: 3100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000767.4, NP_000758.1
• RefSeq Size: 3052 bp
• RefSeq ORF: 1476 bp
• Locus ID: 1555
• Cytogenetics: 19q13.2
• Domains: p450
• Protein Families: Druggable Genome, P450
• Protein Pathways: Arachidonic acid metabolism, Drug metabolism - cytochrome P450, Metabolic pathways, Metabolism of xenobiotics by cytochrome P450, Retinol metabolism
• Gene Summary: 'This gene, CYP2B6, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by phenobarbital. The enzyme is known to metabolize some xenobiotics, such as the anti-cancer drugs cyclophosphamide and ifosphamide. Transcript variants for this gene have been described; however, it has not been resolved whether these transcripts are in fact produced by this gene or by a closely related pseudogene, CYP2B7. Both the gene and the pseudogene are located in the middle of a CYP2A pseudogene found in a large cluster of cytochrome P450 genes from the CYP2A, CYP2B and CYP2F subfamilies on chromosome 19q. [provided by RefSeq, Jul 2008]'