USP30 (NM_032663) Human Untagged Clone

CAT#: SC125494

USP30 (untagged)-Human ubiquitin specific peptidase 30 (USP30)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: USP30
• Synonyms: FLJ40511; MGC10702
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_032663 edited
  CGGCCGCGAATTCGCACGAGGCCGTCGTATCCCTCGGTCCGGCGGCGGCGGCGGCGGTAG
  CGGAGGAGACGGTTTCAGGCCTCCGGTGCGGCTGCAATGCTGAGCTCCCGGGCCGAGGCG
  GCGATGACCGCGGCCGACAGGGCCATCCAGCGCTTCCTGCGGACCGGGGCGGCCGTCAGA
  TATAAAGTCATGAAGAACTGGGGAGTTATAGGTGGAATTGCTGCTGCTCTTGCAGCAGGA
  ATATATGTTATTTGGGGTCCCATTACAGAAAGAAAGAAGCGTAGAAAAGGGCTTGTGCCT
  GGCCTTGTTAATTTAGGGAACACCTGCTTCATGAACTCCCTGCTACAAGGCCTGTCTGCC
  TGTCCTGCTTTCATCAGGTGGCTGGAAGAGTTCACCTCCCAGTACTCCAGGGATCAGAAG
  GAGCCCCCCTCACACCAGTATTTATCCTTAACACTCTTGCACCTTCTGAAAGCCTTGTCC
  TGCCAAGAAGTTACTGATGATGAGGTCTTAGATGCAAGCTGCTTGTTGGATGTCTTAAGA
  ATGTACAGATGGCAGATCTCATCATTTGAAGAACAGGATGCTCACGAATTATTCCATGTC
  ATTACCTCGTCATTGGAAGATGAGCGAGACCGCCAGCCTCGGGTCACACATTTGTTTGAT
  GTGCATTCCCTGGAGCAGCAGTCAGAAATAACTCCCAAACAAATTACCTGCCGCACAAGA
  GGGTCACCTCACCCCACATCCAATCACTGGAAGTCTCAACATCCTTTTCATGGAAGACTC
  ACTAGTAATATGGTCTGCAAACACTGTGAACACCAGAGTCCTGTTCGATTTGATACCTTT
  GATAGCCTTTCACTAAGTATTCCAGCCGCCACATGGGGTCACCCATTGACCCTGGACCAC
  TGCCTTCACCACTTCATCTCATCAGAATCAGTGCGGGATGTTGTGTGTGACAACTGTACA
  AAGATTGAAGCCAAGGGAACGTTGAACGGGGAAAAGGTGGAACACCAGAGGACCACTTTT
  GTTAAACAGTTAAAACTAGGGAAGCTCCCTCAGTGTCTCTGCATCCACCTACAGCGGCTG
  AGCTGGTCCAGCCACGGCACGCCTCTGAAGCGGCATGAGCACGTGCAGTTCAATGAGTTC
  CTGATGATGGACATTTACAAGTACCACCTCCTTGGACATAAACCTAGTCAACACAACCCT
  AAACTGAACAAGAACCCAGGGCCTACACTGGAGCTGCAGGATGGGCCGGGAGCCCCCACA
  CCAGTTCTGAATCAGCCAGGGGCCCCCAAAACACAGATTTTTATGAATGGCGCCTGCTCC
  CCATCTTTATTGCCAACGCTGTCAGCGCCGATGCCCTTCCCTCTCCCAGTTGTTCCCGAC
  TACAGCTCCTCCACATACCTCTTCCGGCTGATGGCAGTTGTCGTCCACCATGGAGACATG
  CACTCTGGACACTTTGTCACTTACCGACGGTCCCCACCTTCTGCCAGGAACCCTCTCTCA
  ACTAGCAATCAGTGGCTGTGGGTCTCCGATGACACTGTCCGCAAGGCCAGCCTGCAGGAG
  GTCCTGTCCTCCAGCGCCTACCTGCTGTTCTACGAGCGCGTCCTTTCCAGGATGCAGCAC
  CAGAGCCAGGAGTGCAAGTCTGAAGAATGACTGTGCCCTCCTGCAAGGCTAGAGCTGATG
  GCACTGTCTGCACTGTCCAGGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_032663 unedited
  CTATAGGGCGGCCGCGAATTCGCACGAGGCCGTCGTATCCCTCGGTCCGGCGGCGGCGGC
  GGCGGTAGCGGAGGAGACGGTTTCAGGCCTCCGGTGCGGCTGCAATGCTGAGCTCCCGGG
  CCGAGGCGGCGATGACCGCGGCCGACAGGGCCATCCAGCGCTTCCTGCGGACCGGGGCGG
  CCGTCAGATATAAAGTCATGAAGAACTGGGGAGTTATAGGTGGAATTGCTGCTGCTCTTG
  CAGCAGGAATATATGTTATTTGGGGTCCCATTACAGAAAGAAAGAAGCGTAGAAAAGGGC
  TTGTGCCTGGCCTTGTTAATTTAGGGAACACCTGCTTCATGAACTCCCTGCTACAAGGCC
  TGTCTGCCTGTCCTGCTTTCATCAGGTGGCTGGAAGAGTTCACCTCCCAGTACTCCAGGG
  ATCAGAAGGAGCCCCCCTCACACCAGTATTTATCCTTAACACTCTTGCACCTTCTGAAAG
  CCTTGTCCTGCCAAGAAGTTACTGATGATGAGGTCTTAAATGCAAGCTGCTTGTTGGATG
  TCTTAAGAATGTACAGATGGCAGATCTCATCATTTGAAGAACAGGATGCTCACGAATTAT
  TCCCATGTCATTACCTCGTCATTGGAAGATGAGCGAGACCGCCAGCCTCGGGTCACACAT
  TTGTTTGATGTGCATTCCCTGGAGCAGCAGTCAGAAATAACTCCCAAACAATTACCTGCC
  GCACAAGAGGTTCACCTTACCCCACATCCAATCACTGGAAGTCTCACCATCCTTTTCATG
  GAAGACTCACTANTAATATGGTCTGCAAACACTGTGAACACCAAAGTCCTGTTCGATTTG
  ATACCTTTGAAAGCCTTTCACTAATATTCCACCCGCCCCATGGGTTCACC
  
  3' Read Nucleotide Sequence

  >Forward primer walk for NM_032663 unedited
  CTTGGACACTGCATTCATGAGTTCCTGNATGATGGACATTTACAAGTACCACCTCCTTGG
  ACATAAACCTAGTCAACACAACCCTAAACTGAACAAGAACCCAGGGCCTACACTGGAGCT
  GCAGGATGGGCCGGGAGCCCCCACACCAGTTCTGAATCAGCCAGGGGCCCCCAAAACACA
  GATTTTTATGAATGGCGCCTGCTCCCCATCTTTATTGCCAACGCTGTCAGCGCCGATGCC
  CTTCCCTCTCCCAGTTGTTCCCGACTACAGCTCCTCCACATACCTCTTCCGGCTGATGGC
  AGTTGTCGTCCACCATGGAGACATGCACTCTGGACACTTTGTCACTTACCGACGGTCCCC
  ACCTTCTGCCAGGAACCCTCTCTCAACTAGCAATCAGTGGCTGTGGGTCTCCGATGACAC
  TGTCCGCAAGGCCAGCCTGCAGGAGGTCCTGTCCTCCAGCGCCTACCTGCTGTTCTACGA
  GCGCGTCCTTTCCAGGATGCAGCACCAGAGCCAGGAGTGCAAGTCTGAAGAATGACTGTG
  CCCTCCTGCAAGGCTAGAGCTGATGGCACTGTCTGCACTGTCCAGGANAAAAGTAAAACT
  GTACTGTTGCGTGTGCAAGCGGCCCCACTAGAGCCTTCCAGCCTTCTGGTGTGTTCTAAG
  AGCAGGCTCCACCTGNGAGCCAGCCCCAGTTCACACCAAACCAGGCTCCCTGAACAGTCC
  TGTTCATGTGTGTAGGTGGTTCTGTTGTGTTAAGAAAGCATTCATTATGTCCGGAGTGTC
  TTTTTACTCATCTGATACAGGGTAATAAAAGAACTCAGATTCTTGAGCCNACGTTTTCAT
  ATTGTATGGTAGGTGTTCTCAGAGGGGAAGTACCTTTGTCTAAATCAACGTTTCACTTAG
  ATCTTTTATTTTTATAAGC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_032663
• ORF Size: 1527 bp
• Insert Size: 4100
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info

Reference Data

• RefSeq: NM_032663.2, NP_116052.1
• RefSeq Size: 2117
• RefSeq ORF: 1527
• Locus ID: 84749
• Domains: UCH
• Protein Families: Protease, Transmembrane
• Gene Summary: USP30, a member of the ubiquitin-specific protease family (see USP1, MIM 603478), is a novel mitochondrial deubiquitinating (DUB) enzyme (Nakamura and Hirose, 2008 [PubMed 18287522]). [supplied by OMIM, Dec 2008]
  Transcript Variant: This variant (1) encodes the longer isoform (1).