GAA (NM_000152) Human Untagged Clone
CAT#: SC125512
GAA (untagged)-Human glucosidase, alpha, acid (GAA), transcript variant 1
"NM_000152" in other vectors (6)
Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Symbol | GAA |
Synonyms | LYAG |
Vector | pCMV6-XL6 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC125512 sequence for NM_000152 edited (data generated by NextGen Sequencing)
ATGGGAGTGAGGCACCCGCCCTGCTCCCACCGGCTCCTGGCCGTCTGCGCCCTCGTGTCC TTGGCAACCGCTGCACTCCTGGGGCACATCCTACTCCATGATTTCCTGCTGGTTCCCCGA GAGCTGAGTGGCTCCTCCCCAGTCCTGGAGGAGACTCACCCAGCTCACCAGCAGGGAGCC AGCAGACCAGGGCCCCGGGATGCCCAGGCACACCCCGGCCGTCCCAGAGCAGTGCCCACA CAGTGCGACGTCCCCCCCAACAGCCGCTTCGATTGCGCCCCTGACAAGGCCATCACCCAG GAACAGTGCGAGGCCCGCGGCTGTTGCTACATCCCTGCAAAGCAGGGGCTGCAGGGAGCC CAGATGGGGCAGCCCTGGTGCTTCTTCCCACCCAGCTACCCCAGCTACAAGCTGGAGAAC CTGAGCTCCTCTGAAATGGGCTACACGGCCACCCTGACCCGTACCACCCCCACCTTCTTC CCCAAGGACATCCTGACCCTGCGGCTGGACGTGATGATGGAGACTGAGAACCGCCTCCAC TTCACGATCAAAGATCCAGCTAACAGGCGCTACGAGGTGCCCTTGGAGACCCCGCATGTC CACAGCCGGGCACCGTCCCCACTCTACAGCGTGGAGTTCTCCGAGGAGCCCTTCGGGGTG ATCGTGCGCCGGCAGCTGGACGGCCGCGTGCTGCTGAACACGACGGTGGCGCCCCTGTTC TTTGCGGACCAGTTCCTTCAGCTGTCCACCTCGCTGCCCTCGCAGTATATCACAGGCCTC GCCGAGCACCTCAGTCCCCTGATGCTCAGCACCAGCTGGACCAGGATCACCCTGTGGAAC CGGGACCTTGCGCCCACGCCCGGTGCGAACCTCTACGGGTCTCACCCTTTCTACCTGGCG CTGGAGGACGGCGGGTCGGCACACGGGGTGTTCCTGCTAAACAGCAATGCCATGGATGTG GTCCTGCAGCCGAGCCCTGCCCTTAGCTGGAGGTCGACAGGTGGGATCCTGGATGTCTAC ATCTTCCTGGGCCCAGAGCCCAAGAGCGTGGTGCAGCAGTACCTGGACGTTGTGGGATAC CCGTTCATGCCGCCATACTGGGGCCTGGGCTTCCACCTGTGCCGCTGGGGCTACTCCTCC ACCGCTATCACCCGCCAGGTGGTGGAGAACATGACCAGGGCCCACTTCCCCCTGGACGTC CAGTGGAACGACCTGGACTACATGGACTCCCGGAGGGACTTCACGTTCAACAAGGATGGC TTCCGGGACTTCCCGGCCATGGTGCAGGAGCTGCACCAGGGCGGCCGGCGCTACATGATG ATCGTGGATCCTGCCATCAGCAGCTCGGGCCCTGCCGGGAGCTACAGGCCCTACGACGAG GGTCTGCGGAGGGGGGTTTTCATCACCAACGAGACCGGCCAGCCGCTGATTGGGAAGGTA TGGCCCGGGTCCACTGCCTTCCCCGACTTCACCAACCCCACAGCCCTGGCCTGGTGGGAG GACATGGTGGCTGAGTTCCATGACCAGGTGCCCTTCGACGGCATGTGGATTGACATGAAC GAGCCTTCCAACTTCATCAGGGGCTCTGAGGACGGCTGCCCCAACAATGAGCTGGAGAAC CCACCCTACGTGCCTGGGGTGGTTGGGGGGACCCTCCAGGCGGCCACCATCTGTGCCTCC AGCCACCAGTTTCTCTCCACACACTACAACCTGCACAACCTCTACGGCCTGACCGAAGCC ATCGCCTCCCACAGGGCGCTGGTGAAGGCTCGGGGGACACGCCCATTTGTGATCTCCCGC TCGACCTTTGCTGGCCACGGCCGATACGCCGGCCACTGGACGGGGGACGTGTGGAGCTCC TGGGAGCAGCTCGCCTCCTCCGTGCCAGAAATCCTGCAGTTTAACCTGCTGGGGGTGCCT CTGGTCGGGGCCGACGTCTGCGGCTTCCTGGGCAACACCTCAGAGGAGCTGTGTGTGCGC TGGACCCAGCTGGGGGCCTTCTACCCCTTCATGCGGAACCACAACAGCCTGCTCAGTCTG CCCCAGGAGCCGTACAGCTTCAGCGAGCCGGCCCAGCAGGCCATGAGGAAGGCCCTCACC CTGCGCTACGCACTCCTCCCCCACCTCTACACACTGTTCCACCAGGCCCACGTCGCGGGG GAGACCGTGGCCCGGCCCCTCTTCCTGGAGTTCCCCAAGGACTCTAGCACCTGGACTGTG GACCACCAGCTCCTGTGGGGGGAGGCCCTGCTCATCACCCCAGTGCTCCAGGCCGGGAAG GCCGAAGTGACTGGCTACTTCCCCTTGGGCACATGGTACGACCTGCAGACGGTGCCAGTA GAGGCCCTTGGCAGCCTCCCACCCCCACCTGCAGCTCCCCGTGAGCCAGCCATCCACAGC GAGGGGCAGTGGGTGACGCTGCCGGCCCCCCTGGACACCATCAACGTCCACCTCCGGGCT GGGTACATCATCCCCCTGCAGGGCCCTGGCCTCACAACCACAGAGTCCCGCCAGCAGCCC ATGGCCCTGGCTGTGGCCCTGACCAAGGGTGGGGAGGCCCGAGGGGAGCTGTTCTGGGAC GATGGAGAGAGCCTGGAAGTGCTGGAGCGAGGGGCCTACACACAGGTCATCTTCCTGGCC AGGAATAACACGATCGTGAATGAGCTGGTACGTGTGACCAGTGAGGGAGCTGGCCTGCAG CTGCAGAAGGTGACTGTCCTGGGCGTGGCCACGGCGCCCCAGCAGGTCCTCTCCAACGGT GTCCCTGTCTCCAACTTCACCTACAGCCCCGACACCAAGGTCCTGGACATCTGTGTCTCG CTGTTGATGGGAGAGCAGTTTCTCGTCAGCTGGTGTTAG Clone variation with respect to NM_000152.3 >OriGene 5' read for NM_000152 unedited
TTTCCCCGCCCGTTGNCGCATTGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCA GAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGA ATTCGGCACGAGGGTGAGCCGGGCCGGGGCTGCGGGGCTTCCCTGAGCGCGGGCCGGGTC GGTGGGGCGGTCGGCTGCCCGCGCGGCCTCTCAGTTGGGAAAGCTGAGGTTGTCGCCGGG GCCGCGGGTGGAGGTCGGGGATGAGGCAGCAGGTAGGACAGTGACCTCGGTGACGCGAAG GACCCCGGCCACCTCTAGGTTCTCCTCGTCCGCCCGTTGTTCAGCGAGGGAGGCTCTGCG CGTGCCGCAGCTGACGGGGAAACTGAGGCACGGAGCGGGCCTGTAGGAGCTGTCCAGGCC ATCTCCAACCATGGGAGTGAGGCACCCGCCCTGCTCCCACCGGCTCCTGGCCGTCTGCGC CCTCGTGTCCTTGGCAACCGCTGCACTCCTGGGGCACATCCTACTCCATGATTTCCTGCT GGTTCCCCGAGAGCTGAGTGGCTCCTCCCCAGTCCTGGAGGAGACTCACCCAGCTCACCA GCAGGGAGCCAGCAGACCAGGGCCCNCGGGATGCCCAGGCACACCCCGGGCCGTCCCAGA GCAGTGCCCACACAGTGCGACGTCCCCCCCACAGCCGCTTCGATTGCGCCCCTGACANGG CCATCACCCAGNAACAGTGCGAGGCCCCGCGGCTGGTGCTACATCCCTGNCAAGCAGGGG CTGCAGGGAGCCCANATGGGGCAGCCTGGTGCTTTTTCCACCAGCTACCCAGCTACAGCT GGAGAACTGAGCTCTCTGAATGGGCTACACGGCACCCTGACCGTACACCCCCACTTCTTC CCAGGACANCTGACCTGCGNNTGACGTGA >OriGene 3' read for NM_000152 unedited
TCNCCCCCAAGATCGTGGNACGCGGNCCCGCATTTTANGANCGNGTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCAAAGCCCCCTTTTATTAAAAATA ATTGCACTTAAAATTTAAAATCTCCCAAGTCCTGGGAATTTTCCTGAGTTCCCAGGCTTG CTCGGGGACCGGCAGGCATCCAGCCCTTTGGGCAGCCGGGCAGCGGTCGCGTTGGGGCAA AGCAACAGGCCCGAAGCAGGAGCTCAGGTGCATACCCCACACCTCCACCTGAGCACCCCC TTTTCCGGTGCTGGGAACAAATGGAGGGGGGTGGCTAATACTACCCGCATGCCGGCAACA GGTGAGGAGGGCAAACCTTACAATCTTATTAACACAGAGCAGCCCTCCAGGGCCCCGGCC CACAGTGCGATCTAGGGAAAAAGCTCTCCTAAACACGTTGGGGGCCAAAGCCCCGGCCCA GGAGGGGGAAACAAGCGATGCGGCGGCTTGGAATGGTTAGTGCCCAGCTCCAGGTGACAC ATGCAACCCCCAGGCCCGCACACAGTTGCTGCCCGCACACAGGCTCTGCTTCCCTGGGGA ACCAGCCTCCCTCTGGAGAGACTAACACACTCCGCCCGGCTAACACCAGCTGACGAGAAA CTGCTCTCCCATCAACAGCGAGACACAGATGTTCCAGGACCTTGGTGTCGGGGCTGTAGG TGAAGTTGGAGACAGGGACACCGTTTGGAAAGGACCTGCTGGGGGCCCCCGGCCCACCCC CAAGAAGTCACCTTCTGGAGGTGCAGGCCAACTCCCTTACTGGTCAAACGTACCAACTTA TTCAGAATCGGGTTATTCCCTGGCCGA |
Restriction Sites | NotI-NotI |
ACCN | NM_000152 |
Insert Size | 3700 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_000152.2, NP_000143.1 |
RefSeq Size | 3846 bp |
RefSeq ORF | 2859 bp |
Locus ID | 2548 |
Cytogenetics | 17q25.3 |
Domains | Glyco_hydro_31, PD |
Protein Families | Druggable Genome, Transmembrane |
Protein Pathways | Galactose metabolism, Lysosome, Metabolic pathways, Starch and sucrose metabolism |
Gene Summary | 'This gene encodes lysosomal alpha-glucosidase, which is essential for the degradation of glycogen to glucose in lysosomes. The encoded preproprotein is proteolytically processed to generate multiple intermediate forms and the mature form of the enzyme. Defects in this gene are the cause of glycogen storage disease II, also known as Pompe's disease, which is an autosomal recessive disorder with a broad clinical spectrum. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]' Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2, and 3 all encode the same protein. |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC215796 | GAA (Myc-DDK-tagged)-Human glucosidase, alpha, acid (GAA), transcript variant 1 |
USD 420.00 |
|
RG215796 | GAA (GFP-tagged) - Human glucosidase, alpha; acid (GAA), transcript variant 1 |
USD 460.00 |
|
RC215796L1 | Lenti ORF clone of Human glucosidase, alpha; acid (GAA), transcript variant 1, Myc-DDK-tagged |
USD 768.00 |
|
RC215796L2 | Lenti ORF clone of Human glucosidase, alpha; acid (GAA), transcript variant 1, mGFP tagged |
USD 620.00 |
|
RC215796L3 | Lenti ORF clone of Human glucosidase, alpha; acid (GAA), transcript variant 1, Myc-DDK-tagged |
USD 620.00 |
|
RC215796L4 | Lenti ORF clone of Human glucosidase, alpha; acid (GAA), transcript variant 1, mGFP tagged |
USD 620.00 |
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