ST14 (NM_021978) Human Untagged Clone

CAT#: SC125523

ST14 (untagged)-Human suppression of tumorigenicity 14 (colon carcinoma) (ST14)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ST14
• Synonyms: ARCI11; HAI; MT-SP1; MTSP1; PRSS14; SNC19; TADG15; TMPRSS14
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC125523 sequence for NM_021978 edited (data generated by NextGen Sequencing)
  ATGGGGAGCGATCGGGCCCGCAAGGGCGGAGGGGGCCCGAAGGACTTCGGCGCGGGACTC
  AAGTACAACTCCCGGCACGAGAAAGTGAATGGCTTGGAGGAAGGCGTGGAGTTCCTGCCA
  GTCAACAACGTCAAGAAGGTGGAAAAGCATGGCCCGGGGCGCTGGGTGGTGCTGGCAGCC
  GTGCTGATCGGCCTCCTCTTGGTCTTGCTGGGGATCGGCTTCCTGGTGTGGCATTTGCAG
  TACCGGGACGTGCGTGTCCAGAAGGTCTTCAATGGCTACATGAGGATCACAAATGAGAAT
  TTTGTGGATGCCTACGAGAACTCCAACTCCACTGAGTTTGTAAGCCTGGCCAGCAAGGTG
  AAGGACGCGCTGAAGCTGCTGTACAGCGGAGTCCCATTCCTGGGCCCCTACCACAAGGAG
  TCGGCTGTGACGGCCTTCAGCGAGGGCAGCGTCATCGCCTACTACTGGTCTGAGTTCAGC
  ATCCCGCAGCACCTGGTGGAGGAGGCCGAGCGCGTCATGGCCGAGGAGCGCGTAGTCATG
  CTGCCCCCGCGGGCGCGCTCCCTGAAGTCCTTTGTGGTCACCTCAGTGGTGGCTTTCCCC
  ACGGACTCCAAAACAGTACAGAGGACCCAGGACAACAGCTGCAGCTTTGGCCTGCACGCC
  CGCGGTGTGGAGCTGATGCGCTTCACCACGCCCGGCTTCCCTGACAGCCCCTACCCCGCT
  CATGCCCGCTGCCAGTGGGCCCTGCGGGGGGACGCCGACTCAGTGCTGAGCCTCACCTTC
  CGCAGCTTTGACCTTGCGTCCTGCGACGAGCGCGGCAGCGACCTGGTGACGGTGTACAAC
  ACCCTGAGCCCCATGGAGCCCCACGCCCTGGTGCAGTTGTGTGGCACCTACCCTCCCTCC
  TACAACCTGACCTTCCACTCCTCCCAGAACGTCCTGCTCATCACACTGATAACCAACACT
  GAGCGGCGGCATCCCGGCTTTGAGGCCACCTTCTTCCAGCTGCCTAGGATGAGCAGCTGT
  GGAGGCCGCTTACGTAAAGCCCAGGGGACATTCAACAGCCCCTACTACCCAGGCCACTAC
  CCACCCAACATTGACTGCACATGGAACATTGAGGTGCCCAACAACCAGCATGTGAAGGTG
  CGCTTCAAATTCTTCTACCTGCTGGAGCCCGGCGTGCCTGCGGGCACCTGCCCCAAGGAC
  TACGTGGAGATCAATGGGGAGAAATACTGCGGAGAGAGGTCCCAGTTCGTCGTCACCAGC
  AACAGCAACAAGATCACAGTTCGCTTCCACTCAGATCAGTCCTACACCGACACCGGCTTC
  TTAGCTGAATACCTCTCCTACGACTCCAGTGACCCATGCCCGGGGCAGTTCACGTGCCGC
  ACGGGGCGGTGTATCCGGAAGGAGCTGCGCTGTGATGGCTGGGCCGACTGCACCGACCAC
  AGCGATGAGCTCAACTGCAGTTGCGACGCCGGCCACCAGTTCACGTGCAAGAACAAGTTC
  TGCAAGCCCCTCTTCTGGGTCTGCGACAGTGTGAACGACTGCGGAGACAACAGCGACGAG
  CAGGGGTGCAGTTGTCCGGCCCAGACCTTCAGGTGTTCCAATGGGAAGTGCCTCTCGAAA
  AGCCAGCAGTGCAATGGGAAGGACGACTGTGGGGACGGGTCCGACGAGGCCTCCTGCCCC
  AAGGTGAACGTCGTCACTTGTACCAAACACACCTACCGCTGCCTCAATGGGCTCTGCTTG
  AGCAAGGGCAACCCTGAGTGTGACGGGAAGGAGGACTGTAGCGACGGCTCAGATGAGAAG
  GACTGCGACTGTGGGCTGCGGTCATTCACGAGACAGGCTCGTGTTGTTGGGGGCACGGAT
  GCGGATGAGGGCGAGTGGCCCTGGCAGGTAAGCCTGCATGCTCTGGGCCAGGGCCACATC
  TGCGGTGCTTCCCTCATCTCTCCCAACTGGCTGGTCTCTGCCGCACACTGCTACATCGAT
  GACAGAGGATTCAGGTACTCAGACCCCACGCAGTGGACGGCCTTCCTGGGCTTGCACGAC
  CAGAGCCAGCGCAGCGCCCCTGGGGTGCAGGAGCGCAGGCTCAAGCGCATCATCTCCCAC
  CCCTTCTTCAATGACTTCACCTTCGACTATGACATCGCGCTGCTGGAGCTGGAGAAACCG
  GCAGAGTACAGCTCCATGGTGCGGCCCATCTGCCTGCCGGACGCCTCCCATGTCTTCCCT
  GCCGGCAAGGCCATCTGGGTCACGGGCTGGGGACACACCCAGTATGGAGGCACTGGCGCG
  CTGATCCTGCAAAAGGGTGAGATCCGCGTCATCAACCAGACCACCTGCGAGAACCTCCTG
  CCGCAGCAGATCACGCCGCGCATGATGTGCGTGGGCTTCCTCAGCGGCGGCGTGGACTCC
  TGCCAGGGTGATTCCGGGGGACCCCTGTCCAGCGTGGAGGCGGATGGGCGGATCTTCCAG
  GCCGGTGTGGTGAGCTGGGGAGACGGCTGCGCTCAGAGGAACAAGCCAGGCGTGTACACA
  AGGCTCCCTCTGTTTCGGGACTGGATCAAAGAGAACACTGGGGTATAG
  
  Clone variation with respect to NM_021978.3
  1215 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_021978 unedited
  GGTGTTCGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGGAATTCGGACGAGCAGG
  GTACGACGCCTGTGAGACCCGCCAGCGGGCTCTGCGTCCATGGGGAGCGATCGGGCCCTC
  CTGGGCGGAGGGGGCCCGAATGACTTCCGCGCGGGACTCAAGCTCAACTACCGGCACGAG
  AAATTGAATGGCTTGTAGGAAGGCGTGTATTTCCTGCCAGTCAACAACGTCAAGAAGTGG
  AAAAGCATGGCCCGGGGCGCTAGGTGGTGCTGGCACCCGTGCTGATCGGCCTACTCTTGG
  TCTTGCTGGGGATCGGCTTTCTGGTGTGGCATTTGCAGTACCGGTACGTGCGTGTCCAGA
  AGGTCTTTAATGGCTACATGATGATCACAAATGAGAATTTTGTGGATGCCTACGAGAACT
  CCAACTCCACTGAGTTTGAAAGCCTGGCCAGCATGGTGAAGGACGCGCTGAAGCTGCTGT
  ACAGCGGAGTCCCATTCCTGGGCCCCTACTCACAAAGAGTCTGCTGTGACGGCCTTCAGC
  GAGTGCAGCGTCATCGCCTACTACTGGTCTGAGTTCAGCATCCCGCAGCACCTGGTGCAG
  GAGGACGATCGCGTCATGGCCCATGAGCGCGTAGTCATGCTGCCCCCGCGGTCGCGCTCC
  CTGAAGTCCTTTGTGGTCACCTCACTGGTGGCTATCCCCACTGACTTCAAAACAGTACAC
  AGGACCCATGACAACAGCTGCAGCTTTTGCCTGCACGCACGCGGTGTGCAGCTGATGCGC
  TTCACCACGCCAGGCTTGCCTGACAGNTCCTACCCGCTCATGCGCGCTAACANTGGGCCC
  TTGCGGGGGACGCCGACTCACTGCTGAGCCTCACCTTTCGCAGCTTTGACCTTGCGTCCT
  GCTACGAGCGCGGCTACGACCTGGAGACCGTGTACAACACCCTGATCCCCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_021978 unedited
  ATCCGTAAGGCCATTGGGCGATGGTCAACTTGCCAGGNCACAGGGAAGAGCACTGGGGGN
  AGGGAGTCACAGGGCATGCCACCCGGGTTCTGTTCAGGAAACAGCTATGACCGCGGCCGC
  AATCTAGAGTCGAGTTTTTTTGTTTTTTTTTTAAAAATAATAAAATGTTTTACTCATTTA
  CACAAATACACACACTGAAGTCCACCCTGGGATCTGGTAAAACAATTTCAGTCTCACACC
  CGTCTGTTTTCCAGGGTCCTCCGAGCCTGGGCTTCCTCAAGAGCGTGGCCCAAGGGCCCC
  ATAGCTCATATCCAGACTCCCCAACACCTTCACTGAAGAGGCCCCTAAGCTCCGTTCCCG
  GTGCTCCTTAAAGACAGGGGAGGCAGATATGCACAAACGCGCCTCGGCCCAGCTTGGGGC
  TGGCGGAGAGAGGCTGTGTCTTCAAACCTTTGCCCCCAGTTGGGTCAGTAAAACCACCAG
  TGTCCTCCCCTTTTACCTCCCAACTTCACTTTGGATGCTGATGAAGCGATAGGTTTTCTA
  GGCATATTTGGAGCCCTGGAGATTGATTTCACAGTGTATGTTCTGGGGGCGCTGGTGCAA
  TCAGCAGTCCAGTCTCCATCCTGCAAGCTGTGCACACTGGTGGTGGACGATGTGGTGGCA
  CCCGCAAGTGTACACATTTTGGGTAGGCCCCGGCCCCTATACCCCAGTGTTCTCTTTGAT
  CCAGTCCCGAAACAGAGGAGCCTTGTGTACACGCCTGGCTTGTTGCTCTGATCGCAGCCG
  TCTTCCCATCTGACCACACCGGACTGGAATATCCGGCCATCCGTCTCCACTCTGGACAGG
  GGTCCCCCCGAATCACGCTGGGCGGATACTAGCCCTGACGTGAGTCCCCT
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_021978
• Insert Size: 3100 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: TrueClone.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021978.2, NP_068813.1
• RefSeq Size: 3269 bp
• RefSeq ORF: 2568 bp
• Locus ID: 6768
• Cytogenetics: 11q24.3
• Domains: CUB, Tryp_SPc, ldl_recept_a
• Protein Families: Druggable Genome, Protease, Transmembrane
• Gene Summary: 'The protein encoded by this gene is an epithelial-derived, integral membrane serine protease. This protease forms a complex with the Kunitz-type serine protease inhibitor, HAI-1, and is found to be activated by sphingosine 1-phosphate. This protease has been shown to cleave and activate hepatocyte growth factor/scattering factor, and urokinase plasminogen activator, which suggest the function of this protease as an epithelial membrane activator for other proteases and latent growth factors. The expression of this protease has been associated with breast, colon, prostate, and ovarian tumors, which implicates its role in cancer invasion, and metastasis. [provided by RefSeq, Jul 2008]'