ST14 (NM_021978) Human Untagged Clone

CAT#: SC125523

ST14 (untagged)-Human suppression of tumorigenicity 14 (colon carcinoma) (ST14)


  "NM_021978" in other vectors (7)

Reconstitution Protocol

USD 310.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol ST14
Synonyms ARCI11; HAI; MT-SP1; MTSP1; PRSS14; SNC19; TADG15; TMPRSS14
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC125523 sequence for NM_021978 edited (data generated by NextGen Sequencing)
ATGGGGAGCGATCGGGCCCGCAAGGGCGGAGGGGGCCCGAAGGACTTCGGCGCGGGACTC
AAGTACAACTCCCGGCACGAGAAAGTGAATGGCTTGGAGGAAGGCGTGGAGTTCCTGCCA
GTCAACAACGTCAAGAAGGTGGAAAAGCATGGCCCGGGGCGCTGGGTGGTGCTGGCAGCC
GTGCTGATCGGCCTCCTCTTGGTCTTGCTGGGGATCGGCTTCCTGGTGTGGCATTTGCAG
TACCGGGACGTGCGTGTCCAGAAGGTCTTCAATGGCTACATGAGGATCACAAATGAGAAT
TTTGTGGATGCCTACGAGAACTCCAACTCCACTGAGTTTGTAAGCCTGGCCAGCAAGGTG
AAGGACGCGCTGAAGCTGCTGTACAGCGGAGTCCCATTCCTGGGCCCCTACCACAAGGAG
TCGGCTGTGACGGCCTTCAGCGAGGGCAGCGTCATCGCCTACTACTGGTCTGAGTTCAGC
ATCCCGCAGCACCTGGTGGAGGAGGCCGAGCGCGTCATGGCCGAGGAGCGCGTAGTCATG
CTGCCCCCGCGGGCGCGCTCCCTGAAGTCCTTTGTGGTCACCTCAGTGGTGGCTTTCCCC
ACGGACTCCAAAACAGTACAGAGGACCCAGGACAACAGCTGCAGCTTTGGCCTGCACGCC
CGCGGTGTGGAGCTGATGCGCTTCACCACGCCCGGCTTCCCTGACAGCCCCTACCCCGCT
CATGCCCGCTGCCAGTGGGCCCTGCGGGGGGACGCCGACTCAGTGCTGAGCCTCACCTTC
CGCAGCTTTGACCTTGCGTCCTGCGACGAGCGCGGCAGCGACCTGGTGACGGTGTACAAC
ACCCTGAGCCCCATGGAGCCCCACGCCCTGGTGCAGTTGTGTGGCACCTACCCTCCCTCC
TACAACCTGACCTTCCACTCCTCCCAGAACGTCCTGCTCATCACACTGATAACCAACACT
GAGCGGCGGCATCCCGGCTTTGAGGCCACCTTCTTCCAGCTGCCTAGGATGAGCAGCTGT
GGAGGCCGCTTACGTAAAGCCCAGGGGACATTCAACAGCCCCTACTACCCAGGCCACTAC
CCACCCAACATTGACTGCACATGGAACATTGAGGTGCCCAACAACCAGCATGTGAAGGTG
CGCTTCAAATTCTTCTACCTGCTGGAGCCCGGCGTGCCTGCGGGCACCTGCCCCAAGGAC
TACGTGGAGATCAATGGGGAGAAATACTGCGGAGAGAGGTCCCAGTTCGTCGTCACCAGC
AACAGCAACAAGATCACAGTTCGCTTCCACTCAGATCAGTCCTACACCGACACCGGCTTC
TTAGCTGAATACCTCTCCTACGACTCCAGTGACCCATGCCCGGGGCAGTTCACGTGCCGC
ACGGGGCGGTGTATCCGGAAGGAGCTGCGCTGTGATGGCTGGGCCGACTGCACCGACCAC
AGCGATGAGCTCAACTGCAGTTGCGACGCCGGCCACCAGTTCACGTGCAAGAACAAGTTC
TGCAAGCCCCTCTTCTGGGTCTGCGACAGTGTGAACGACTGCGGAGACAACAGCGACGAG
CAGGGGTGCAGTTGTCCGGCCCAGACCTTCAGGTGTTCCAATGGGAAGTGCCTCTCGAAA
AGCCAGCAGTGCAATGGGAAGGACGACTGTGGGGACGGGTCCGACGAGGCCTCCTGCCCC
AAGGTGAACGTCGTCACTTGTACCAAACACACCTACCGCTGCCTCAATGGGCTCTGCTTG
AGCAAGGGCAACCCTGAGTGTGACGGGAAGGAGGACTGTAGCGACGGCTCAGATGAGAAG
GACTGCGACTGTGGGCTGCGGTCATTCACGAGACAGGCTCGTGTTGTTGGGGGCACGGAT
GCGGATGAGGGCGAGTGGCCCTGGCAGGTAAGCCTGCATGCTCTGGGCCAGGGCCACATC
TGCGGTGCTTCCCTCATCTCTCCCAACTGGCTGGTCTCTGCCGCACACTGCTACATCGAT
GACAGAGGATTCAGGTACTCAGACCCCACGCAGTGGACGGCCTTCCTGGGCTTGCACGAC
CAGAGCCAGCGCAGCGCCCCTGGGGTGCAGGAGCGCAGGCTCAAGCGCATCATCTCCCAC
CCCTTCTTCAATGACTTCACCTTCGACTATGACATCGCGCTGCTGGAGCTGGAGAAACCG
GCAGAGTACAGCTCCATGGTGCGGCCCATCTGCCTGCCGGACGCCTCCCATGTCTTCCCT
GCCGGCAAGGCCATCTGGGTCACGGGCTGGGGACACACCCAGTATGGAGGCACTGGCGCG
CTGATCCTGCAAAAGGGTGAGATCCGCGTCATCAACCAGACCACCTGCGAGAACCTCCTG
CCGCAGCAGATCACGCCGCGCATGATGTGCGTGGGCTTCCTCAGCGGCGGCGTGGACTCC
TGCCAGGGTGATTCCGGGGGACCCCTGTCCAGCGTGGAGGCGGATGGGCGGATCTTCCAG
GCCGGTGTGGTGAGCTGGGGAGACGGCTGCGCTCAGAGGAACAAGCCAGGCGTGTACACA
AGGCTCCCTCTGTTTCGGGACTGGATCAAAGAGAACACTGGGGTATAG

Clone variation with respect to NM_021978.3
1215 c=>t
>OriGene 5' read for NM_021978 unedited
GGTGTTCGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGGAATTCGGACGAGCAGG
GTACGACGCCTGTGAGACCCGCCAGCGGGCTCTGCGTCCATGGGGAGCGATCGGGCCCTC
CTGGGCGGAGGGGGCCCGAATGACTTCCGCGCGGGACTCAAGCTCAACTACCGGCACGAG
AAATTGAATGGCTTGTAGGAAGGCGTGTATTTCCTGCCAGTCAACAACGTCAAGAAGTGG
AAAAGCATGGCCCGGGGCGCTAGGTGGTGCTGGCACCCGTGCTGATCGGCCTACTCTTGG
TCTTGCTGGGGATCGGCTTTCTGGTGTGGCATTTGCAGTACCGGTACGTGCGTGTCCAGA
AGGTCTTTAATGGCTACATGATGATCACAAATGAGAATTTTGTGGATGCCTACGAGAACT
CCAACTCCACTGAGTTTGAAAGCCTGGCCAGCATGGTGAAGGACGCGCTGAAGCTGCTGT
ACAGCGGAGTCCCATTCCTGGGCCCCTACTCACAAAGAGTCTGCTGTGACGGCCTTCAGC
GAGTGCAGCGTCATCGCCTACTACTGGTCTGAGTTCAGCATCCCGCAGCACCTGGTGCAG
GAGGACGATCGCGTCATGGCCCATGAGCGCGTAGTCATGCTGCCCCCGCGGTCGCGCTCC
CTGAAGTCCTTTGTGGTCACCTCACTGGTGGCTATCCCCACTGACTTCAAAACAGTACAC
AGGACCCATGACAACAGCTGCAGCTTTTGCCTGCACGCACGCGGTGTGCAGCTGATGCGC
TTCACCACGCCAGGCTTGCCTGACAGNTCCTACCCGCTCATGCGCGCTAACANTGGGCCC
TTGCGGGGGACGCCGACTCACTGCTGAGCCTCACCTTTCGCAGCTTTGACCTTGCGTCCT
GCTACGAGCGCGGCTACGACCTGGAGACCGTGTACAACACCCTGATCCCCT
>OriGene 3' read for NM_021978 unedited
ATCCGTAAGGCCATTGGGCGATGGTCAACTTGCCAGGNCACAGGGAAGAGCACTGGGGGN
AGGGAGTCACAGGGCATGCCACCCGGGTTCTGTTCAGGAAACAGCTATGACCGCGGCCGC
AATCTAGAGTCGAGTTTTTTTGTTTTTTTTTTAAAAATAATAAAATGTTTTACTCATTTA
CACAAATACACACACTGAAGTCCACCCTGGGATCTGGTAAAACAATTTCAGTCTCACACC
CGTCTGTTTTCCAGGGTCCTCCGAGCCTGGGCTTCCTCAAGAGCGTGGCCCAAGGGCCCC
ATAGCTCATATCCAGACTCCCCAACACCTTCACTGAAGAGGCCCCTAAGCTCCGTTCCCG
GTGCTCCTTAAAGACAGGGGAGGCAGATATGCACAAACGCGCCTCGGCCCAGCTTGGGGC
TGGCGGAGAGAGGCTGTGTCTTCAAACCTTTGCCCCCAGTTGGGTCAGTAAAACCACCAG
TGTCCTCCCCTTTTACCTCCCAACTTCACTTTGGATGCTGATGAAGCGATAGGTTTTCTA
GGCATATTTGGAGCCCTGGAGATTGATTTCACAGTGTATGTTCTGGGGGCGCTGGTGCAA
TCAGCAGTCCAGTCTCCATCCTGCAAGCTGTGCACACTGGTGGTGGACGATGTGGTGGCA
CCCGCAAGTGTACACATTTTGGGTAGGCCCCGGCCCCTATACCCCAGTGTTCTCTTTGAT
CCAGTCCCGAAACAGAGGAGCCTTGTGTACACGCCTGGCTTGTTGCTCTGATCGCAGCCG
TCTTCCCATCTGACCACACCGGACTGGAATATCCGGCCATCCGTCTCCACTCTGGACAGG
GGTCCCCCCGAATCACGCTGGGCGGATACTAGCCCTGACGTGAGTCCCCT
Restriction Sites ECoRI-NOT     
ACCN NM_021978
Insert Size 3100 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation TrueClone.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_021978.2, NP_068813.1
RefSeq Size 3269 bp
RefSeq ORF 2568 bp
Locus ID 6768
Cytogenetics 11q24.3
Domains CUB, Tryp_SPc, ldl_recept_a
Protein Families Druggable Genome, Protease, Transmembrane
Gene Summary 'The protein encoded by this gene is an epithelial-derived, integral membrane serine protease. This protease forms a complex with the Kunitz-type serine protease inhibitor, HAI-1, and is found to be activated by sphingosine 1-phosphate. This protease has been shown to cleave and activate hepatocyte growth factor/scattering factor, and urokinase plasminogen activator, which suggest the function of this protease as an epithelial membrane activator for other proteases and latent growth factors. The expression of this protease has been associated with breast, colon, prostate, and ovarian tumors, which implicates its role in cancer invasion, and metastasis. [provided by RefSeq, Jul 2008]'

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