BCL2 (NM_000633) Human Untagged Clone

CAT#: SC125546

BCL2 (untagged)-Human B-cell CLL/lymphoma 2 (BCL2), nuclear gene encoding mitochondrial protein, transcript variant alpha

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: BCL2
• Synonyms: Bcl-2; PPP1R50
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_000633, the custom clone sequence may differ by one or more nucleotides
  
  
  GAATTCCCGGGATATCGTCGACCCACGCGTCCGCTGGAGAGTGCTGAAGATTGATGGGATCGTTGCCTTA
  TGCATTTGTTTTGGTTTTACAAAAAGGAAACTTGACAGAGGATCATGCTGTACTTAAAAAATACAACATC
  ACAGAGGAAGTAGACTGATATTAACAATACTTACTAATAATAACGTGCCTCATGAAATAAAGATCCGAAA
  GGAATTGGAATAAAAATTTCCTGCATCTCATGCCAAGGGGGAAACACCAGAATCAAGTGTTCCGCGTGAT
  TGAAGACACCCCCTCGTCCAAGAATGCAAAGCACATCCAATAAAATAGCTGGATTATAACTCCTCTTCTT
  TCTCTGGGGGCCGTGGGGTGGGAGCTGGGGCGAGAGGTGCCGTTGGCCCCCGTTGCTTTTCCTCTGGGAA
  GGATGGCGCACGCTGGGAGAACGGGGTACGATAACCGGGAGATAGTGATGAAGTACATCCATTATAAGCT
  GTCGCAGAGGGGCTACGAGTGGGATGCGGGAGATGTGGGCGCCGCGCCCCCGGGGGCCGCCCCCGCACCG
  GGCATCTTCTCCTCCCAGCCCGGGCACACGCCCCATCCAGCCGCATCCCGGGACCCGGTCGCCAGGACCT
  CGCCGCTGCAGACCCCGGCTGCCCCCGGCGCCGCCGCGGGGCCTGCGCTCAGCCCGGTGCCACCTGTGGT
  CCACCTGACCCTCCGCCAGGCCGGCGACGACTTCTCCCGCCGCTACCGCCGCGACTTCGCCGAGATGTCC
  AGCCAGCTGCACCTGACGCCCTTCACCGCGCGGGGACGCTTTGCCACGGTGGTGGAGGAGCTCTTCAGGG
  ACGGGGTGAACTGGGGGAGGATTGTGGCCTTCTTTGAGTTCGGTGGGGTCATGTGTGTGGAGAGCGTCAA
  CCGGGAGATGTCGCCCCTGGTGGACAACATCGCCCTGTGGATGACTGAGTACCTGAACCGGCACCTGCAC
  ACCTGGATCCAGGATAACGGAGGCTGGGATGCCTTTGTGGAACTGTACGGCCCCAGCATGCGGCCTCTGT
  TTGATTTCTCCTGGCTGTCTCTGAAGACTCTGCTCAGTTTGGCCCTGGTGGGAGCTTGCATCACCCTGGG
  TGCCTATCTGGGCCACAAGTGAAGTCAACATGCCTGCCCCAAACAAATATGCAAAAGGTTCACTAAAGCA
  GTAGAAATAATATGCATTGTCAGTGATGTACCATGAAACAAAGCTGCAGGCTGTTTAAGAAAAAATAACA
  CACATATAAACATCACACACACAGACAGACACACACACACACAACAATTAACAGTCTTCAGGCAAAACGT
  CGAATCAGCTATTTACTGCCAAAGGGAAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000633 unedited
  NGTTCACGTTAAAATTTGTAATAGACTCACTATAGGCGGCCGCGNAATCAGATCTGGTAC
  CGGTCCGGAATTCCCGGGATATCGTCGACCCACGCGTCCGCTGGAGAGTGCTGAAGATTG
  ATGGGATCGTTGCCTTATGCATTTGTTTTGGTTTTACAAAAAGGAAACTTGACAGAGGAT
  TCATGCTGTACTTAAAAAATACAACATCACAGAGGAAGTAGACTGATATTAACAATACTT
  ACTAATAATAACGTGCCTCATGAAATAAAGATCCGAAAGGAATTGGAATAAAAATTTCCT
  GCATCTCATGCCAAGGGGGAAACACCAGAATCAAGTGTTCCGCGTGATTGAAGACACCCC
  CTCGTCCAAGAATGCAAAGCACATCCAATAAAATAGCTGGATTATAACTCCTCTTCTTTC
  TCTGGGGGCCGTGGGGTGGGAGCTGGGGCGAGAGGTGCCGTTGGCCCCCGTTGCTTTTCC
  TCTGGGAAGGATGGCGCACGCTGGGAGAACGGGGTACGATAACCGGGAGATAGTGATGAA
  GTACATCCATTATAAGCTGTCGCAGAGGGGCTACGAGTGGGATGCCGGAGAATGTTGGCG
  CCGCGCCCCCGGGGGCCGCCCCCGCACCGGGCATCTTCTCCTCCCAGCCCGGGCACACGC
  CCCATCCAGCCGCATCCCGGGACCCGGTCGCCAGGACCTCGCCGCTGCAGACCCCCGCTG
  CCCNCGGCGCGGCCGCGGGGGCCTGCGCTCAGCCCGGTGCCACCCTGTGTCCACCTGACC
  CTCGCCAGCCNGCGACGACTTCTCCGCCGCTACCGCCGCGACTTCGCCGAGATGTTCAGC
  CAGCTGCACCTGACGCCTTTACCGCGCCGGGGACGCTTTGCACCGTGGGGTGGAAGAGCT
  CTTCAGGNACGGGGTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000633 unedited
  CTGGGCCATTGGNGATGGCAACTTCCAGGNCCAGNAAAGCACTGGGGNAGGGTCACAGGN
  ATGCCACCCGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCCCTTTTTTTTTTTTT
  TTAATGCCCCAGGATGTACAGATAACCCCCATATTCCACACCTGGAACTTTTTTTTTGTC
  AGGTTTTCAAATAAAACCAAACTACAGTGACAAGATAATGTTTTACATGTAATTCCATAG
  ACAGGGGTCAATTAATCCATGACACCTCACTTCAATGCTTCTTTTAGGATTTGTGACCCT
  CTCCAAAGTCATTTAAAGCCTTGCTTTAAACTCACAGGTGGGCCAAGGCCACACAGCCAA
  CGTGCCATGTGCTACAGCCAAAATGGGCCGTGGCCATTGCCTCTCCTCACGTTCCCAGCC
  TTCACCATGTCCTTCTGATAGGAAGGGCCCAGGGCCTCTGTTCCTTCCCTCTACAGTGAT
  ACATGTCTTAAGAAGGGTCGTGGCTCCCATGCTCCACGTGAAAACGGGCCTACCTGGAGG
  GCCCCAGGGTGACAGGCCCAGCCACACCCCTCTACTGCTCTTTCTTGACAGTGGAATTCT
  GAGCTCCATCAGCTTCCAGACATTCGGAGACCACACTGCCCTGTTGATCATCCCTGGAGG
  AGGCCAGTGAGGGCCCCGGCTCAGTTCCAGGACCAGGCCTCCAAGCTGGGACACAGGCAG
  GTTCTGCGGACTTCGGTCTCCTAAAACAGGCACTTGTGGCGGCCTGATGCTCTGGGTAAC
  TCTAGCCTTCCTGATGCGGAAGTCACCGAAATGTTCACTTCCTCAAGTTCCAGAGGATTC
  TGTTTCTTACTCAGACAGAGCCAGTATTGGGAGTTGGGGGGGGTGCGTATCCAAAATATA
  TGAATATACACAATCG
  
• Restriction Sites: Please inquire
• ACCN: NM_000633
• Insert Size: 3050 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000633.2, NP_000624.2
• RefSeq Size: 6492 bp
• RefSeq ORF: 720 bp
• Locus ID: 596
• Cytogenetics: 18q21.33
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Stem cell - Pluripotency, Transmembrane
• Protein Pathways: Amyotrophic lateral sclerosis (ALS), Apoptosis, Colorectal cancer, Focal adhesion, Neurotrophin signaling pathway, Pathways in cancer, Prostate cancer, Small cell lung cancer
• Gene Summary: 'This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]'
  Transcript Variant: This variant (alpha) represents the longer transcript and encodes the longer isoform (alpha).