Caspase 8 (CASP8) (NM_033355) Human Untagged Clone

CAT#: SC125549

CASP8 (untagged)-Human caspase 8, apoptosis-related cysteine peptidase (CASP8), transcript variant B


  "NM_033355" in other vectors (4)

Reconstitution Protocol

USD 810.00

In Stock*

Size
    • 10 ug

Product Images

Other products for "CASP8"

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol CASP8
Synonyms ALPS2B; CAP4; Casp-8; FLICE; MACH; MCH5
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_033355, the custom clone sequence may differ by one or more nucleotides


ATGGACTTCAGCAGAAATCTTTATGATATTGGGGAACAACTGGACAGTGAAGATCTGGCCTCCCTCAAGT
TCCTGAGCCTGGACTACATTCCGCAAAGGAAGCAAGAACCCATCAAGGATGCCTTGATGTTATTCCAGAG
ACTCCAGGAAAAGAGAATGTTGGAGGAAAGCAATCTGTCCTTCCTGAAGGAGCTGCTCTTCCGAATTAAT
AGACTGGATTTGCTGATTACCTACCTAAACACTAGAAAGGAGGAGATGGAAAGGGAACTTCAGACACCAG
GCAGGGCTCAAATTTCTGCCTACAGGGTCATGCTCTATCAGATTTCAGAAGAAGTGAGCAGATCAGAATT
GAGGTCTTTTAAGTTTCTTTTGCAAGAGGAAATCTCCAAATGCAAACTGGATGATGACATGAACCTGCTG
GATATTTTCATAGAGATGGAGAAGAGGGTCATCCTGGGAGAAGGAAAGTTGGACATCCTGAAAAGAGTCT
GTGCCCAAATCAACAAGAGCCTGCTGAAGATAATCAACGACTATGAAGAATTCAGCAAAGAGAGAAGCAG
CAGCCTTGAAGGAAGTCCTGATGAATTTTCAAATGGGGAGGAGTTGTGTGGGGTAATGACAATCTCGGAC
TCTCCAAGAGAACAGGATAGTGAATCACAGACTTTGGACAAAGTTTACCAAATGAAAAGCAAACCTCGGG
GATACTGTCTGATCATCAACAATCACAATTTTGCAAAAGCACGGGAGAAAGTGCCCAAACTTCACAGCAT
TAGGGACAGGAATGGAACACACTTGGATGCAGGGGCTTTGACCACGACCTTTGAAGAGCTTCATTTTGAG
ATCAAGCCCCACGATGACTGCACAGTAGAGCAAATCTATGAGATTTTGAAAATCTACCAACTCATGGACC
ACAGTAACATGGACTGCTTCATCTGCTGTATCCTCTCCCATGGAGACAAGGGCATCATCTATGGCACTGA
TGGACAGGAGGCCCCCATCTATGAGCTGACATCTCAGTTCACTGGTTTGAAGTGCCCTTCCCTTGCTGGA
AAACCCAAAGTGTTTTTTATTCAGGCTTGTCAGGGGGATAACTACCAGAAAGGTATACCTGTTGAGACTG
ATTCAGAGGAGCAACCCTATTTAGAAATGGATTTATCATCACCTCAAACGAGATATATCCCGGATGAGGC
TGACTTTCTGCTGGGGATGGCCACTGTGAATAACTGTGTTTCCTACCGAAACCCTGCAGAGGGAACCTGG
TACATCCAGTCACTTTGCCAGAGCCTGAGAGAGCGATGTCCTCGAGGCGATGATATTCTCACCATCCTGA
CTGAAGTGAACTATGAAGTAAGCAACAAGGATGACAAGAAAAACATGGGGAAACAGATGCCTCAGCCTAC
TTTCACACTAAGAAAAAAACTTGTCTTCCCTTCTGATTGA


>OriGene 5' read for NM_033355 unedited
AGATTTGGAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTGGTTGTTCA
GACTGAGCTTCCTGCCTGCCTGTACCCCGCCAACAGCTTCAGAAGAAGGTGACTGGTGGC
TGCCTGAGGAATACCAGTGGGCAAGAGAATTAGCATTTCTGGAGCATCTGCTGTCTGAGC
AGCCCCTGGGTGCGTCCACTTTCTGGGCACGTGAGGTTGGGCCTTGGCCGCCTGAGCCCT
TGAGTTGGTCACTTGAACCTTGGGAATATTGAGATTATATTCTCCTGCCTTTTAAAAAGA
TGGACTTCAGCAGAAATCTTTATGATATTGGGGAACAACTGGACAGTGAAGATCTGGCCT
CCCTCAAGTTCCTGAGCCTGGACTACATTCCGCAAAGGAAGCAAGAACCCATCAAGGATG
CCTTGATGTTATTCCAGAGACTCCAGGAAAAGAGAATGTTGGAGGAAAGCAATCTGTCCT
TCCTGAAGGAGCTGCTCTTCCGAATTAATAGACTGGATTTGCTGATTACCTACCTAAACA
CTAGAAAGGAGGAGATGGAAAGGGAACTTCAGACACCAGGCAGGGCTCANATTTCTGCCT
ACAGGGTCATGCTCTATCAGATTTCAGAAGAAGTGAGCAGATCAGAAATGAGGTCCTTTA
NGTTTCTTTTGCAAGAGGAAATCTCCAAATGCAAACTGGATGATGACATGAACCCTGCCT
GGAATTTTTCATAGAGATTGAGAAAAAGGTCATTCTGGGGAAAAAGAAGTGGAACAACCT
TAGAAAGATCTGCGCCCAATCAACAGAAGCCGGTGAATATACCCCCCCTTAAAATTTCCC
AGGGGGGGGATTGGTGGGGAGCAACCCGACTTCCCAAACAGGAAGTATCCAACTTGTGAG
GTTCA
>OriGene 3' read for NM_033355 unedited
GGAGTGCACTTCCAGGCCAGGAGAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGA
TCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTGGGT
TTTTTTAAGATCAAATTATATTTTATTCTATTAAAAAATGTTTTTGAAAAAAGATACTTA
AATTTTAAAGATAACTTAATTCCTAATGATTTAAAATAATCCAAGCAGAGATGAAAGAGC
AAATGCAAATGCATAAAAAGACCCCAGAGCATTGTTAGCAAAAAGCAAATATAGTTAGCC
AAGCATATATATATCATAAAAGCAATAAGAAGGCATAAAGCAAGTTTGGGGAGAGCTTAT
TTAAAACTTGTAAAAATCATTTGAATTTTTAAAAGTTTTCAAACAAATTTTGTTTTATTA
AAAAAAAAATTTTTTTTTGAGACGGGGTCTCGTTCTGTCACCCAAGCTTGAGTGCAGCGG
TGTGAACATGGCTCACCGCAGCCTTGACCTCTTGGGTTCAAGCAATCCTCCCACCTCAAC
CTCCCAAAGTGCTGGGACCATAGACATGAGCCACCATACGTGGCTACTTTCTAATTTTTT
TAATGTGGTAGTGTGGAATTTAGCTTTGAAAGAGAATTAAAGTTAATAGAATATTAACAG
TGGGCTTATAGTTGATAGATAACAATCTGAATCATAAGCCCTCTATATTCATAGCTGGAT
TTTGTACGTGTCTGGTATTTATTGCCACCAGCTAAAAAACATCCCAAGAGCCTTAGCTTA
ANAAAAAGGAAACGGGGTATTGTGGTAAATCCCATTAACCTTGCCTTATAACAGGCAAAC
ACTTATTAGTGAAATCTTNAATATAATAAACTAGGTATTCAAAAAGCTCTGCCGGCGTGC
TTCG
Restriction Sites NotI-NotI     
ACCN NM_033355
Insert Size 4400 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_033355.2, NP_203519.1
RefSeq Size 2908 bp
RefSeq ORF 1440 bp
Locus ID 841
Cytogenetics 2q33.1
Domains DED, CASc, ICE_p10, ICE_p20
Protein Families Druggable Genome, Protease
Protein Pathways Alzheimer's disease, Apoptosis, Huntington's disease, NOD-like receptor signaling pathway, p53 signaling pathway, Pathways in cancer, RIG-I-like receptor signaling pathway, Toll-like receptor signaling pathway, Viral myocarditis
Gene Summary 'This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined. [provided by RefSeq, Jul 2008]'
Transcript Variant: This variant (B) includes different segments in its 5' UTR and lacks a 5' coding region segment, compared to variant G. This results in translation at a downstream start codon, and the encoded protein (isoform B) has a shorter N-terminus when it is compared to isoform G. CCDS Note: This CCDS representation is based on AF422928.1 and U58143.1, and on data in PMID:11917123. This 479 aa isoform is also supported by homologous proteins, including pig NP_001026949.1, cow NP_001039435.1, mouse NP_033942.1, rat NP_071613.1 and chicken NP_989923.1. In addition, macaque AB172864.1 supports the full-length splice pattern.

Other Versions

{0} Product Review(s)

0 Product Review(s) Submit review

Be the first one to submit a review

Product Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.