Caspase 8 (CASP8) (NM_033355) Human Untagged Clone

CAT#: SC125549

CASP8 (untagged)-Human caspase 8, apoptosis-related cysteine peptidase (CASP8), transcript variant B

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CASP8
• Synonyms: ALPS2B; CAP4; Casp-8; FLICE; MACH; MCH5
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_033355, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGACTTCAGCAGAAATCTTTATGATATTGGGGAACAACTGGACAGTGAAGATCTGGCCTCCCTCAAGT
  TCCTGAGCCTGGACTACATTCCGCAAAGGAAGCAAGAACCCATCAAGGATGCCTTGATGTTATTCCAGAG
  ACTCCAGGAAAAGAGAATGTTGGAGGAAAGCAATCTGTCCTTCCTGAAGGAGCTGCTCTTCCGAATTAAT
  AGACTGGATTTGCTGATTACCTACCTAAACACTAGAAAGGAGGAGATGGAAAGGGAACTTCAGACACCAG
  GCAGGGCTCAAATTTCTGCCTACAGGGTCATGCTCTATCAGATTTCAGAAGAAGTGAGCAGATCAGAATT
  GAGGTCTTTTAAGTTTCTTTTGCAAGAGGAAATCTCCAAATGCAAACTGGATGATGACATGAACCTGCTG
  GATATTTTCATAGAGATGGAGAAGAGGGTCATCCTGGGAGAAGGAAAGTTGGACATCCTGAAAAGAGTCT
  GTGCCCAAATCAACAAGAGCCTGCTGAAGATAATCAACGACTATGAAGAATTCAGCAAAGAGAGAAGCAG
  CAGCCTTGAAGGAAGTCCTGATGAATTTTCAAATGGGGAGGAGTTGTGTGGGGTAATGACAATCTCGGAC
  TCTCCAAGAGAACAGGATAGTGAATCACAGACTTTGGACAAAGTTTACCAAATGAAAAGCAAACCTCGGG
  GATACTGTCTGATCATCAACAATCACAATTTTGCAAAAGCACGGGAGAAAGTGCCCAAACTTCACAGCAT
  TAGGGACAGGAATGGAACACACTTGGATGCAGGGGCTTTGACCACGACCTTTGAAGAGCTTCATTTTGAG
  ATCAAGCCCCACGATGACTGCACAGTAGAGCAAATCTATGAGATTTTGAAAATCTACCAACTCATGGACC
  ACAGTAACATGGACTGCTTCATCTGCTGTATCCTCTCCCATGGAGACAAGGGCATCATCTATGGCACTGA
  TGGACAGGAGGCCCCCATCTATGAGCTGACATCTCAGTTCACTGGTTTGAAGTGCCCTTCCCTTGCTGGA
  AAACCCAAAGTGTTTTTTATTCAGGCTTGTCAGGGGGATAACTACCAGAAAGGTATACCTGTTGAGACTG
  ATTCAGAGGAGCAACCCTATTTAGAAATGGATTTATCATCACCTCAAACGAGATATATCCCGGATGAGGC
  TGACTTTCTGCTGGGGATGGCCACTGTGAATAACTGTGTTTCCTACCGAAACCCTGCAGAGGGAACCTGG
  TACATCCAGTCACTTTGCCAGAGCCTGAGAGAGCGATGTCCTCGAGGCGATGATATTCTCACCATCCTGA
  CTGAAGTGAACTATGAAGTAAGCAACAAGGATGACAAGAAAAACATGGGGAAACAGATGCCTCAGCCTAC
  TTTCACACTAAGAAAAAAACTTGTCTTCCCTTCTGATTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_033355 unedited
  AGATTTGGAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTGGTTGTTCA
  GACTGAGCTTCCTGCCTGCCTGTACCCCGCCAACAGCTTCAGAAGAAGGTGACTGGTGGC
  TGCCTGAGGAATACCAGTGGGCAAGAGAATTAGCATTTCTGGAGCATCTGCTGTCTGAGC
  AGCCCCTGGGTGCGTCCACTTTCTGGGCACGTGAGGTTGGGCCTTGGCCGCCTGAGCCCT
  TGAGTTGGTCACTTGAACCTTGGGAATATTGAGATTATATTCTCCTGCCTTTTAAAAAGA
  TGGACTTCAGCAGAAATCTTTATGATATTGGGGAACAACTGGACAGTGAAGATCTGGCCT
  CCCTCAAGTTCCTGAGCCTGGACTACATTCCGCAAAGGAAGCAAGAACCCATCAAGGATG
  CCTTGATGTTATTCCAGAGACTCCAGGAAAAGAGAATGTTGGAGGAAAGCAATCTGTCCT
  TCCTGAAGGAGCTGCTCTTCCGAATTAATAGACTGGATTTGCTGATTACCTACCTAAACA
  CTAGAAAGGAGGAGATGGAAAGGGAACTTCAGACACCAGGCAGGGCTCANATTTCTGCCT
  ACAGGGTCATGCTCTATCAGATTTCAGAAGAAGTGAGCAGATCAGAAATGAGGTCCTTTA
  NGTTTCTTTTGCAAGAGGAAATCTCCAAATGCAAACTGGATGATGACATGAACCCTGCCT
  GGAATTTTTCATAGAGATTGAGAAAAAGGTCATTCTGGGGAAAAAGAAGTGGAACAACCT
  TAGAAAGATCTGCGCCCAATCAACAGAAGCCGGTGAATATACCCCCCCTTAAAATTTCCC
  AGGGGGGGGATTGGTGGGGAGCAACCCGACTTCCCAAACAGGAAGTATCCAACTTGTGAG
  GTTCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_033355 unedited
  GGAGTGCACTTCCAGGCCAGGAGAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGA
  TCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTGGGT
  TTTTTTAAGATCAAATTATATTTTATTCTATTAAAAAATGTTTTTGAAAAAAGATACTTA
  AATTTTAAAGATAACTTAATTCCTAATGATTTAAAATAATCCAAGCAGAGATGAAAGAGC
  AAATGCAAATGCATAAAAAGACCCCAGAGCATTGTTAGCAAAAAGCAAATATAGTTAGCC
  AAGCATATATATATCATAAAAGCAATAAGAAGGCATAAAGCAAGTTTGGGGAGAGCTTAT
  TTAAAACTTGTAAAAATCATTTGAATTTTTAAAAGTTTTCAAACAAATTTTGTTTTATTA
  AAAAAAAAATTTTTTTTTGAGACGGGGTCTCGTTCTGTCACCCAAGCTTGAGTGCAGCGG
  TGTGAACATGGCTCACCGCAGCCTTGACCTCTTGGGTTCAAGCAATCCTCCCACCTCAAC
  CTCCCAAAGTGCTGGGACCATAGACATGAGCCACCATACGTGGCTACTTTCTAATTTTTT
  TAATGTGGTAGTGTGGAATTTAGCTTTGAAAGAGAATTAAAGTTAATAGAATATTAACAG
  TGGGCTTATAGTTGATAGATAACAATCTGAATCATAAGCCCTCTATATTCATAGCTGGAT
  TTTGTACGTGTCTGGTATTTATTGCCACCAGCTAAAAAACATCCCAAGAGCCTTAGCTTA
  ANAAAAAGGAAACGGGGTATTGTGGTAAATCCCATTAACCTTGCCTTATAACAGGCAAAC
  ACTTATTAGTGAAATCTTNAATATAATAAACTAGGTATTCAAAAAGCTCTGCCGGCGTGC
  TTCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_033355
• Insert Size: 4400 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033355.2, NP_203519.1
• RefSeq Size: 2908 bp
• RefSeq ORF: 1440 bp
• Locus ID: 841
• Cytogenetics: 2q33.1
• Domains: DED, CASc, ICE_p10, ICE_p20
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Alzheimer's disease, Apoptosis, Huntington's disease, NOD-like receptor signaling pathway, p53 signaling pathway, Pathways in cancer, RIG-I-like receptor signaling pathway, Toll-like receptor signaling pathway, Viral myocarditis
• Gene Summary: 'This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (B) includes different segments in its 5' UTR and lacks a 5' coding region segment, compared to variant G. This results in translation at a downstream start codon, and the encoded protein (isoform B) has a shorter N-terminus when it is compared to isoform G. CCDS Note: This CCDS representation is based on AF422928.1 and U58143.1, and on data in PMID:11917123. This 479 aa isoform is also supported by homologous proteins, including pig NP_001026949.1, cow NP_001039435.1, mouse NP_033942.1, rat NP_071613.1 and chicken NP_989923.1. In addition, macaque AB172864.1 supports the full-length splice pattern.