PRKACA (NM_002730) Human Untagged Clone

CAT#: SC125607

PRKACA (untagged)-Human protein kinase, cAMP-dependent, catalytic, alpha (PRKACA), transcript variant 1


  "NM_002730" in other vectors (6)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

Product Images

Other products for "PRKACA"

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol PRKACA
Synonyms PKACA; PPNAD4
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC125607 sequence for NM_002730 edited (data generated by NextGen Sequencing)
ATGGGCAACGCCGCCGCCGCCAAGAAGGGCAGCGAGCAGGAGAGCGTGAAAGAATTCTTA
GCCAAAGCCAAAGAAGATTTTCTTAAAAAATGGGAAAGTCCCGCTCAGAACACAGCCCAC
TTGGATCAGTTTGAACGAATCAAGACCCTCGGCACGGGCTCCTTCGGGCGGGTGATGCTG
GTGAAACACAAGGAGACCGGGAACCACTATGCCATGAAGATCCTCGACAAACAGAAGGTG
GTGAAACTGAAACAGATCGAACACACCCTGAATGAAAAGCGCATCCTGCAAGCTGTCAAC
TTTCCGTTCCTCGTCAAACTCGAGTTCTCCTTCAAGGACAACTCAAACTTATACATGGTC
ATGGAGTACGTGCCCGGCGGGGAGATGTTCTCACACCTACGGCGGATCGGAAGGTTCAGT
GAGCCCCATGCCCGTTTCTACGCGGCCCAGATCGTCCTGACCTTTGAGTATCTGCACTCG
CTGGATCTCATCTACAGGGACCTGAAGCCGGAGAATCTGCTCATTGACCAGCAGGGCTAC
ATTCAGGTGACAGACTTCGGTTTCGCCAAGCGCGTGAAGGGCCGCACTTGGACCTTGTGC
GGCACCCCTGAGTACCTGGCCCCTGAGATTATCCTGAGCAAAGGCTACAACAAGGCCGTG
GACTGGTGGGCCCTGGGGGTTCTTATCTATGAAATGGCCGCTGGCTACCCGCCCTTCTTC
GCAGACCAGCCCATCCAGATCTATGAGAAGATCGTCTCTGGGAAGGTGCGCTTCCCTTCC
CACTTCAGCTCTGACTTGAAGGACCTGCTGCGGAACCTCCTGCAGGTAGATCTCACCAAG
CGCTTTGGGAACCTCAAGAATGGGGTCAACGATATCAAGAACCACAAGTGGTTTGCCACA
ACTGACTGGATTGCCATCTACCAGAGGAAGGTGGAAGCTCCCTTCATACCAAAGTTTAAA
GGCCCTGGGGATACGAGTAACTTTGACGACTATGAGGAAGAAGAAATCCGGGTCTCCATC
AATGAGAAGTGTGGCAAGGAGTTTTCTGAGTTTTAG

Clone variation with respect to NM_002730.3
>OriGene 5' read for NM_002730 unedited
GGGATTTTGTAATACGACTCACTATAGGGCGGCCGCAATCTAGAGCGGGCGCGGAGAGAC
GCGGGAAGCAGGGGCTGGGCGGGGGTCGCGGCGCCGCAGCTAGCGCAGCCAGCCCGAGGG
CCGCCGCCGCCGCCGCCCAGCGCGCTCCGGGGCCGCCGGCCGCAGCCAGCACCCGCCGCG
CCGCAGCTCCGGGACCGGCCCCGGCCGCCGCCGCCGCGATGGGCAACGCCGCCGCCGCCA
AGAAGGGCAGCGAGCAGGAGAGCGTGAAAGAATTCTTAGCCAAAGCCAAAGAAGATTTTC
TTAAAAAATGGGAAAGTCCCGCTCAGAACACAGCCCACTTGGATCAGTTTGAACGAATCA
AGACCCTCGGCACGGGCTCCTTCGGGCGGGTGATGCTGGTGAAACACAAGGAGACCGGGA
ACCACTATGCCATGAAGATCCTCGACAAACAGAAGGTGGTGAAACTGAAACAGATCGAAC
ACACCCTGAATGAAAAGCGCATCCTGCAAGCTGTCAACTTTCCGTTCCTCGTCAAACTCG
AGTTCTCCTTCAAGGACAACTCANACTTATACATGGTCATGGAGTACGTGCCCGGCGGGG
AGATGTTCTCACACCTACCGCGGATCCGAAAGTTCAGTGAGCCCCATGCCCGTTTCTACC
CGGGCCAGATCGTCCTGGCCTTTGAGTATCTGCCCTCGCTGGATCCTATCTACAGGGACC
TGGAGCCGGAGGATCTGCTCATTGACCAGCAGGGCTACATTCAGGGACAGACTCGGGTTT
GCCAAACGCGGGAAGGGCCCACTGGACCCTGGGCGGGACCCCGGAGACCTGGCCCCTGGA
TTATCCGAGCAAAGGTACACAGGCCCGGGACGGGGGCCCCGGGGGCCTTATTATAGAAAG
GCGCTGGTCACCCCCTTTTCGGAACAGCCCTCCCAATTATGAAAAACGCTT
>OriGene 3' read for NM_002730 unedited
CGGCCGCGAATTCGGCACGAGGTGGAACTTAAATAAGATTTTAAATTGTTGTTTTTTTAA
AAAAATTCTAGCAAGCAACCCACTGAACATGTCACTAAAAATCTCTCCTTCCCAGGCAGG
ATTACTCCGAAAGGAAGGTTGGCGCTTCGTTCATTTGCCCTTAGCAAGTGGGGCCTGTGG
TTGGGTGGGATGGGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
Restriction Sites Please inquire     
ACCN NM_002730
Insert Size 1800 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002730.3, NP_002721.1
RefSeq Size 2689 bp
RefSeq ORF 1056 bp
Locus ID 5566
Cytogenetics 19p13.12
Domains pkinase, S_TK_X, TyrKc, S_TKc
Protein Families Druggable Genome, Protein Kinase
Protein Pathways Apoptosis, Calcium signaling pathway, Chemokine signaling pathway, Dilated cardiomyopathy, Gap junction, GnRH signaling pathway, Hedgehog signaling pathway, Insulin signaling pathway, Long-term potentiation, MAPK signaling pathway, Melanogenesis, Olfactory transduction, Oocyte meiosis, Prion diseases, Progesterone-mediated oocyte maturation, Taste transduction, Vascular smooth muscle contraction, Vibrio cholerae infection, Wnt signaling pathway
Gene Summary 'This gene encodes one of the catalytic subunits of protein kinase A, which exists as a tetrameric holoenzyme with two regulatory subunits and two catalytic subunits, in its inactive form. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. cAMP-dependent phosphorylation of proteins by protein kinase A is important to many cellular processes, including differentiation, proliferation, and apoptosis. Constitutive activation of this gene caused either by somatic mutations, or genomic duplications of regions that include this gene, have been associated with hyperplasias and adenomas of the adrenal cortex and are linked to corticotropin-independent Cushing's syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. Tissue-specific isoforms that differ at the N-terminus have been described, and these isoforms may differ in the post-translational modifications that occur at the N-terminus of some isoforms. [provided by RefSeq, Jan 2015]'
Transcript Variant: This variant (1) encodes Calpha1 (PMID:21812984).

Other Versions

{0} Product Review(s)

0 Product Review(s) Submit review

Be the first one to submit a review

Product Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.