Myosin Light Chain 2 (MYL2) (BC015821) Human Untagged Clone

CAT#: SC125614

MYL2 (untagged)-Human myosin, light polypeptide 2, regulatory, cardiac, slow, mRNA (cDNA clone MGC:13576 IMAGE:4245364), complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MYL2
• Synonyms: cardiac ventricular myosin light chain 2; CMH10; DKFZp779C0562; MLC2; myosin, light polypeptide 2, regulatory, cardiac, slow; myosin light chain 2; myosin regulatory light chain 2, ventricular/cardiac muscle isoform; regulatory light chain; RLC of myosin
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for BC015821 edited
  GGGGGGCGGAGTGTGGAATTCTTCTCGGGAGGCAGTGCTGGGTCCTTTCCACCATGGCAC
  CTAAGAAAGCAAAGAAGAGAGCCGGGGGCGCCAACTCCAACGTGTTCTCCATGTTCGAAC
  AGACCCAAATCCAGGAATTTAAGGAGGCCTTCACTATCATGGACCAGAACAGGGATGGCT
  TCATTGACAAGAACGATCTGAGAGACACCTTTGCTGCCCTTGGGCGAGTGAACGTGAAAA
  ATGAAGAAATTGATGAAATGATCAAGGAGGCTCCGGGTCCAATTAACTTTACTGTGTTCC
  TCACAATGTTTGGGGAGAAACTTAAGGGAGCGGACCCTGAGGAAACCATTCTCAACGCAT
  TCAAAGTGTTTGACCCTGAAGGCAAAGGGGTGCTGAAGGCTGATTACGTTCGGGAAATGC
  TGACCACGCAGGCGGAGAGGTTTTCCAAGGAGGAGGTTGACCAGATGTTCGCCGCCTTCC
  CCCCTGACGTGACTGGCAACTTGGACTACAAGAACCTGGTGCACATCATCACCCACGGAG
  AAGAGAAGGACTAGGAGGGGGCTCGCTGCTGCGCCCTGGGCTCGTCTTTGCAGAGTGGTC
  CCTGCCCTCATCTCTCTCCCCCGAGTACCGCCTCTGTCCCTACCTTGTCTGTTAGCCATG
  TGGCTGCCCCATTTATCCACCTCCATCTTCTTTGCAGCCTGGGTGGCTATGGGTACTTCG
  TGGCCGCACATCCTACAGTTGGAAATCCATCCAGAGGCCATGTTCCAATAAACAGGAGGT
  CGTGTATTTGGTCACGACATTTCTCTGATAAAAAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC015821 unedited
  NCCCGTCAGAATTTGTATACGACTCATATAGGGCGGCCGCGAATCTTCTCGGGNAGCAGT
  GCTGGGTCCTTTCCACCATGGCACCTAAGAAAGCAAAGAAGAGAGCCGGGGGCGCCAACT
  CCAACGTGTTCTCCATGTTCGAACAGACCCAAATCCAGGAATTTAAGGAGGCCTTCACTA
  TCATGGACCAGAACAGGGATGGCTTCATTGACAAGAACGATCTGAGAGACACCTTTGCTG
  CCCTTGGGCGAGTGAACGTGAAAAATGAAGAAATTGATGAAATGATCAAGGAGGCTCCGG
  GTCCAATTAACTTTACTGTGTTCCTCACAATGTTTGGGGAGAAACTTAAGGGAGCGGACC
  CTGAGGAAACCATTCTCAACGCATTCAAAGTGTTTGACCCTGAAGGCAAAGGGGTGCTGA
  AGGCTGATTACGTTCGGGAAATGCTGACCACGCAGGCGGAGAGGTTTTCCAAGGAGGAGG
  TTGACCAGATGTTCGCCGCCTTCCCCCCTGACGTGACTGGCAACTTGGACTACAAGAACC
  TGGTGCACATCATCACCCACGGAGAAGAGAAGGACTAGGAGGGGGCTCGCTGCTGCGCCC
  TGNGCTCGTCTTTGCAGAGTGGTCCCTGCCCTCATCTCTCTCCCCCGAGTACCGCCTCTG
  TCCCTACCTTGTCTGTTAGCCATGTGGCTGCCCCATTTATCCACCTCCATCTTCTTTGCA
  GCCTGGGTGGCTATGGGTACTTCGTGGCCGCACATCCTACAGTTGGAAATCCATCCAGAG
  GCCATGTTCCANATAACAGGGAGTCGTGTATTTGGTCACGACATTTCTCTGATNNNNAAA
  AANNNNNNNNNNNAAAACATGTCGGCCCGCTCGGCCCTCGAGAAGCTTTCTAGATTGCGG
  CCGCGG
  
• Restriction Sites: Please inquire
• ACCN: BC015821
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC015821.1, AAH15821.1
• RefSeq Size: 834 bp
• Locus ID: 4633
• Cytogenetics: 12q24.11
• Protein Pathways: Cardiac muscle contraction, Dilated cardiomyopathy, Focal adhesion, Hypertrophic cardiomyopathy (HCM), Leukocyte transendothelial migration, Regulation of actin cytoskeleton, Tight junction
• Gene Summary: 'Thus gene encodes the regulatory light chain associated with cardiac myosin beta (or slow) heavy chain. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in this gene are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy. [provided by RefSeq, Jul 2008]'