PFKL (NM_001002021) Human Untagged Clone

CAT#: SC125729

PFKL (untagged)-Human phosphofructokinase, liver (PFKL), transcript variant 1


  "NM_001002021" in other vectors (4)

Reconstitution Protocol

USD 1,920.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol PFKL
Synonyms ATP-PFK; PFK-B; PFK-L
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for NM_001002021 edited
CGTGTTTCGGCCGCCGCCATGGCCGCGGTGGACCTGGAGAAGCTGCGGGCGTCGGGCGCG
GGCAAGGCCATCGGCGTCCTGACCAGCGGCGGCGACGCGCAAGGTCCCCTGACAAGCCCA
CCAGGCCCCCTGCTGAGATGGCTGTGACCCTGGGCTGACCCGCCCAGTGGCACATTGACT
CCGCCTGGAGCTGGGGAGACCAGAGAGGCCCTGTGGTTGGACGGTGGCCTGGGTGCGCTG
CTCCTGCCCTCTCCTTGCCCTGCCTCAGCTGCTGCCTGCCAGAGGCGTGGCACCTCACCT
CACACCTGCTCCCTGCTGCTGAGCCCCACGCCAAGCTGGAGAGCGGATGAGAAGCATGTG
TAACCAGGGTAGAGGTCGAGAGTCCTCTCGTGGGGGTCTCCATGTTCAAGGGAGCTGCCG
AGGCTTGAGCAGGAGCCCCCAGCAGGAAACTGGCTTTGCCAAGGCCCCCGCTGGGACAGA
CTGTTTCTTTCACTGCAGTCCTGGGAGCCGAGGGCAAGGGGACAGGAAAGAGGAAGTGAC
CTCAGAGCCTGGTGGCACCAGCATCATGTCCAGGCTGGGGGGCATGAACGCTGCTGTCCG
GGCTGTGACGCGCATGGGCATTTATGTGGGTGCCAAAGTCTTCCTCATCTACGAGGGCTA
TGAGGGCCTCGTGGAGGGAGGTGAGAACATCAAGCAGGCCAACTGGCTGAGCGTCTCCAA
CATCATCCAGCTGGGCGGCACTATCATTGGCAGCGCTCGCTGCAAGGCCTTTACCACCAG
GGAGGGGCGCCGGGCAGCGGCCTACAACCTGGTCCAGCACGGCATCACCAACCTGTGCGT
CATCGGCGGGGATGGCAGCCTTACAGGTGCCAACATCTTCCGCAGCGAGTGGGGCAGCCT
GCTGGAGGAGCTGGTGGCGGAAGGTAAGATCTCAGAGACTACAGCCCGGACCTACTCGCA
CCTGAACATCGCGGGCCTAGTGGGCTCCATCGATAACGACTTCTGCGGCACCGACATGAC
CATCGGCACGGACTCGGCCCTCCACCGCATCATGGAGGTCATCGATGCCATCACCACCAC
TGCCCAGAGCCACCAGAGGACCTTCGTGCTGGAAGTGATGGGCCGGCACTGCGGGTACCT
GGCGCTGGTATCTGCACTGGCCTCAGGGGCCGACTGGCTGTTCATCCCCGAGGCTCCACC
CGAGGACGGCTGGGAGAACTTCATGTGTGAGAGGCTGGGTGAGACTCGGAGCCGTGGGTC
CCGACTGAACATCATCATCATCGCTGAGGGTGCCATTGACCGCAACGGGAAGCCCATCTC
GTCCAGCTACGTGAAGGACCTGGTGGTTCAGAGGCTGGGCTTCGACACCCGTGTAACTGT
GCTGGGCCACGTGCAGCGGGGAGGGACGCCCTCTGCCTTCGACCGGATCCTGAGCAGCAA
GATGGGCATGGAGGCGGTGATGGCGCTGCTGGAAGCCACGCCTGACACGCCGGCCTGCGT
GGTCACCCTCTCGGGGAACCAGTCAGTGCGGCTGCCCCTCATGGAGTGCGTGCAGATGAC
CAAGGAAGTGCAGAAAGCCATGGATGACAAGAGGTTTGACGAGGCCACCCAGCTCCGTGG
TGGGAGCTTCGAGAACAACTGGAACATTTACAAGCTCCTCGCCCACCAGAAGCCCCCCAA
GGAGAAGTCTAACTTCTCCCTGGCCATCCTGAATGTGGGGGCCCCGGCGGCTGGCATGAA
TGCGGCCGTGCGCTCGGCGGTGCGGACCGGCATCTCCCATGGACACACAGTATACGTGGT
GCACGATGGCTTCGAAGGCCTAGCCAAGGGTCAGGTGCAAGAAGTAGGCTGGCACGACGT
GGCCGGCTGGTTGGGGCGTGGTGGCTCCATGCTGGGGACCAAGAGGACCCTGCCCAAGGG
CCAGCTGGAGTCCATTGTGGAGAACATCCGCATCTATGGTATTCACGCCCTGCTGGTGGT
CGGTGGGTTTGAGGCCTATGAAGGGGTGCTGCAGCTGGTGGAGGCTCGCGGGCGCTACGA
GGAGCTCTGCATCGTCATGTGTGTCATCCCAGCCACCATCAGCAACAACGTCCCTGGCAC
CGACTTCAGCCTGGGCTCCGACACTGCTGTAAATGCCGCCATGGAGAGCTGTGACCGCAT
CAAACAGTCTGCCTCGGGGACCAAGCGCCGTGTGTTCATCGTGGAGACCATGGGGGGTTA
CTGTGGCTACCTGGCCACCGTGACTGGCATTGCTGTGGGGGCCGACGCCGCCTACGTCTT
CGAGGACCCTTTCAACATCCACGACTTAAAGGTCAACGTGGAGCACATGACGGAGAAGAT
GAAGACAGACATTCAGAGGGGCCTGGTGCTGCGGAACGAGAAGTGCCATGACTACTACAC
CACGGAGTTCCTGTACAACCTGTACTCATCAGAGGGCAAGGGCGTCTTCGACTGCAGGAC
CAATGTCCTGGGCCACCTGCAGCAGGGTGGCGCTCCAACCCCCTTTGACCGGAACTATGG
GACCAAGCTGGGGGTGAAGGCCATGCTGTGGTTGTCGGAGAAGCTGCGCGAGGTTTACCG
CAAGGGACGGGTGTTCGCCAATGCCCCAGACTCGGCCTGCGTGATCGGCCTGAAGAAGAA
GGCGGTGGCCTTCAGCCCCGTCACTGAGCTCAAGAAAGACACTGATTTCGAGCACCGCAT
GCCACGGGAGCAGTGGTGGCTGAGCCTGCGGCTCATGCTGAAGATGCTGGCACAATACCG
CATCAGTATGGCCGCCTACGTGTCAGGGGAGCTGGAGCACGTGACCCGCCGCACCCTGAG
CATGGACAAGGGCTTCTGAGGCCAGCCATGCCCACGCCCCTCCCCAGCCCCCACCCATGC
CAGCGCAGCGCCAGGGCTCAGATGGGGCCTGGGCTGTTGTGTCTGGAGCCTGCAGGCAGG
TGGGGGCTGCGTCCCTGCTCAGCCCATCCCCTGCCTCTATCCCTGGCCACCTGCCAGGCC
TCCCTCCGGCTGGTGTCTTGAGACCAGCCTGCCAGGCCCTCCAGCAGGAGGACAGAGTGC
CCTGGGGCATCCACCTTCCTGCCCAGGGGACGTGGCGCTGTCGGTGTTTGGAGGCTGCTG
CCCCCTGGCTTTGGCGCCCCATGGGCCCTCAGCGTCTCCCCATGCTGGGCTCACTACATG
GGCCAGCCCTTGCTCTACCTGGCCGGTAGGCTGCTGGCGCCTAGGTTGTGTTGAGAGGGG
GATGCCCCTGGCCCTGCCTCACTGTGACCTGCTCCTGCCCACGTGCAGCACCTGTCACCT
TTTCTAGAAATAAAATCACCCTGACTGTGGGGTGCATCGGTCTCCGGAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAA
>OriGene 5' read for NM_001002021 unedited
AGGTCGGATTTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGCGTGTTTC
GGCCGCCGCCATGGCCGCGGTGGACCTGGAGAAGCTGCGGGCGTCGGGCGCGGGCAAGGC
CATCGGCGTCCTGACCAGCGGCGGCGACGCGCAAGGTCCCCTGACAAGCCCACCAGGCCC
CCTGCTGAGATGGCTGTGACCCTGGGCTGACCCGCCCAGTGGCACATTGACTCCGCCTGG
AGCTGGGGAGACCAGAGAGGCCCTGTGGTTGGACGGTGGCCTGGGTGCGCTGCTCCTGCC
CTCTCCTTGCCCTGCCTCAGCTGCTGCCTGCCAGAGGCGTGGCACCTCACCTCACACCTG
CTCCCTGCTGCTGAGCCCCACGCCAAGCTGGAGAGCGGATGAGAAGCATGTGTAACCAGG
GTAGAGGTCGAGAGTCCTCTCGTGGGGGTCTCCATGTTCAAGGGAGCTGCCGAGGCTTGA
GCAGGAGCCCCCAGCAGGAAACTGGCTTTGCCAAGGCCCCCGCTGGGACAGACTGTTTCT
TTCACTGCAGTCCTGGGAGCCGAGGGCAAGGGGACAGGAAAGAGGAAGTGACCTCAGAGC
CTGGTGGCACCAGCATCATGTCCAGGCTGGGGGGCATGAACGCTGCTGTCCGGGCTGTGA
CGCGCATGGGCATTTATGTGGGTGCCAAAGTCTTCCTCATCTACGAGGGCTATGAGGGCC
TCGTGGAGGGAGGTGAGAACATCAAGCAGGCCAACTGGCTGAGCGTCTCCAACATCATCC
AGCTGGGCGGCACTATCATTGGCAGCGCTCGCTGCAGGCCTTTACCACCAGGAGGGGCGC
CGGGCACGGCCTACACCTGGTCAGCACGCATCACCACCTGTGCGTCATCGGGGGGATGGC
AGCTACAGTGCCACATCT
Restriction Sites Please inquire     
ACCN NM_001002021
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001002021.1, NP_001002021.1
RefSeq Size 3402 bp
RefSeq ORF 2484 bp
Locus ID 5211
Cytogenetics 21q22.3
Protein Families Druggable Genome
Protein Pathways Fructose and mannose metabolism, Galactose metabolism, Glycolysis / Gluconeogenesis, Metabolic pathways, Pentose phosphate pathway
Gene Summary 'This gene encodes the liver (L) subunit of an enzyme that catalyzes the conversion of D-fructose 6-phosphate to D-fructose 1,6-bisphosphate, which is a key step in glucose metabolism (glycolysis). This enzyme is a tetramer that may be composed of different subunits encoded by distinct genes in different tissues. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]'
Transcript Variant: This variant (1) represents the longer transcript and encodes the shorter isoform (a).

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