Metallothionein (MT1A) (BC029475) Human Untagged Clone

CAT#: SC125765

MT1A (untagged)-Human metallothionein 1A (functional), mRNA (cDNA clone MGC:32848 IMAGE:4723818), complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MT1A
• Synonyms: metallothionein 1A; metallothionein 1A (functional); metallothionein 1S; MGC32848; MT1; MT1S; MTC
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for BC029475 edited
  AAGCCTTCCACGTGCGCCTTATAGCCTCTCAACTTCTTGCTTGGGATCTCCAACCTCACC
  GCGGCTCGAAATGGACCCCAACTGCTCCTGCGCCACTGGTGGCTCCTGCACCTGCACTGG
  CTCCTGCAAATGCAAAGAGTGCAAATGCAACTCCTGCAAGAAGAGCTGCTGCTCCTGCTG
  CCCCATGAGCTGTGCCAAGTGTGCCCAGGGCTGCATCTGCAAAGGGGCATCAGAGAAGTG
  CAGCTGCTGTGCCTGATGTCCGGACAGCCCTGCTCGAAGATATAGAAAGAGTGACCTGCA
  CAAACTTGGAATTTTTTTTCCATACAACCCTGACCCATTTACTGTATTTTTTTTAATGAA
  ATATGTGAATGATAATAAAAGTTGCTGACTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC029475 unedited
  CCCCGGTCAGAATTTGTATACCACTGCACTATAGGCGGCCGCGCAATTCGCCATTACGGC
  CGGGGAAGCCTTCCACGTGCGCCTTATAGCCTCTCAACTTCTTGCTTGGGATCTCCAACC
  TCACCGCGGCTCGAAATGGACCCCAACTGCTCCTGCGCCACTGGTGGCTCCTGCACCTGC
  ACTGGCTCCTGCAAATGCAAAGAGTGCAAATGCAACTCCTGCAAGAAGAGCTGCTGCTCC
  TGCTGCCCCATGAGCTGTGCCAAGTGTGCCCAGGGCTGCATCTGCAAAGGGGCATCAGAG
  AAGTGCAGCTGCTGTGCCTGATGTCCGGACAGCCCTGCTCTAAGATATAGAAAGAGTGAC
  CTGCACAAACTTGGAATTTTTTTTCCATACAACCCTGACCCATTTACTGTATTTTTTTTA
  ATGAAATATGTGAATGATAATAAAAGTTGCTGACTTNNNNAAAAAAAAGAAAAACAAAAA
  CACGAAAAACCCCAACCTCCNAGCCCATAAAAGTAAAAAAAAAACCTGTGGGCCGGTTCC
  GCCCTTAACTCTAATTTCCGCCCCCCGGCATAGTTGTTCCCTTAACACATCCCGGGGGGG
  TTCCCTGGAACCTTTCCCCAGGGGTTTTTTTGGCCCCGGAATTTCCCATTCTTTTTCCCC
  CCCCTCTTTCGCCCCACCCAATTTACATTCCCCCCCCTCGTGTTGACCAGGGGGTCCCTC
  TATAAAATTCCGCGCTGGACGGGGGCCCCATTGGCCCCCAGGCGCAATTTCCCAAAAACC
  ACCTTTTTTTTTTTTTCCCCCTTTTTCCCCCCCCATTCTTTTTTCCCCACCCCCTCCCTT
  TTTCCCCCCCACCTCTTTCCCCCTACGTTCCACCCCCCCCCCTTTTTCACCCCCCCCTAN
  
• Restriction Sites: Please inquire
• ACCN: BC029475
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC029475.1, AAH29475.1
• RefSeq Size: 465 bp
• Locus ID: 4489
• Cytogenetics: 16q13
• Gene Summary: 'This gene is a member of the metallothionein family of genes. Proteins encoded by this gene family are low in molecular weight, are cysteine-rich, lack aromatic residues, and bind divalent heavy metal ions. The conserved cysteine residues co-ordinate metal ions using mercaptide linkages. These proteins act as anti-oxidants, protect against hydroxyl free radicals, are important in homeostatic control of metal in the cell, and play a role in detoxification of heavy metals. Disruption of two metallothionein genes in mouse resulted in defects in protection against heavy metals, oxidative stress, immune reactions, carcinogens, and displayed obesity. [provided by RefSeq, Sep 2017]'