Calcium independent Phospholipase A2 (PLA2G6) (NM_001004426) Human Untagged Clone

CAT#: SC125777

PLA2G6 (untagged)-Human phospholipase A2, group VI (cytosolic, calcium-independent) (PLA2G6), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PLA2G6
• Synonyms: CaI-PLA2; GVI; INAD1; iPLA2; IPLA2-VIA; iPLA2beta; NBIA2; NBIA2A; NBIA2B; PARK14; PLA2; PNPLA9
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001004426 edited
  GGAAGTAGAAGTGCTGAGTAAGCCGAGACAGAGGGGGAAGACGGTGGGGCCTCCCCACCT
  GCCCCGCAGAAGATGCAGTTCTTTGGCCGCCTGGTCAATACCTTCAGTGGCGTCACCAAC
  TTGTTCTCTAACCCATTCCGGGTGAAGGAGGTGGCTGTAGCCGACTACACCTCGAGTGAC
  CGAGTTCGGGAGGAAGGGCAGCTGATTCTGTTCCAGAACACTCCCAACCGCACCTGGGAC
  TGCGTCCTGGTCAACCCCAGGAACTCACAGAGTGGATTCCGACTCTTCCAGCTGGAGTTG
  GAGGCTGACGCCCTAGTGAATTTCCATCAGTATTCTTCCCAGCTGCTACCCTTCTATGAG
  AGCTCCCCTCAGGTCCTGCACACTGAGGTCCTGCAGCACCTGACCGACCTCATCCGTAAC
  CACCCCAGCTGGTCAGTGGCCCACCTGGCTGTGGAGCTAGGGATCCGCGAGTGCTTCCAT
  CACAGCCGTATCATCAGCTGTGCCAATTGCGCGGAGAACGAGGAGGGCTGCACACCCCTG
  CACCTGGCCTGCCGCAAGGGTGATGGGGAGATCCTGGTGGAGCTGGTGCAGTACTGCCAC
  ACTCAGATGGATGTCACCGACTACAAGGGAGAGACCGTCTTCCATTATGCTGTCCAGGGT
  GACAATTCTCAGGTGCTGCAGCTCCTTGGAAGGAACGCAGTGGCTGGCCTGAACCAGGTG
  AATAACCAAGGGCTGACCCCGCTGCACCTGGCCTGCCAGCTGGGGAAGCAGGAGATGGTC
  CGCGTGCTGCTGCTGTGCAATGCTCGGTGCAACATCATGGGCCCCAACGGCTACCCCATC
  CACTCGGCCATGAAGTTCTCTCAGAAGGGGTGTGCGGAGATGATCATCAGCATGGACAGC
  AGCCAGATCCACAGCAAAGACCCCCGTTACGGAGCCAGCCCCCTCCACTGGGCCAAGAAC
  GCAGAGATGGCCCGCATGCTGCTGAAACGGGGCTGCAACGTGAACAGCACCAGCTCCGCG
  GGGAACACGGCCCTGCACGTGGCGGTGATGCGCAACCGCTTCGACTGTGCCATAGTGCTG
  CTGACCCACGGGGCCAACGCGGATGCCCGCGGAGAGCACGGCAACACCCCGCTGCACCTG
  GCCATGTCGAAAGACAACGTGGAGATGATCAAGGCCCTCATCGTGTTCGGAGCAGAAGTG
  GACACCCCGAATGACTTTGGGGAGACTCCTACATTCCTAGCCTCCAAAATCGGCAGACAA
  CTACAGGATCTCATGCACATCTCACGGGCCCGGAAGCCAGCGTTCATCCTGGGCTCCATG
  AGGGACGAGAAGCGGACCCACGACCACCTGCTGTGCCTGGATGGAGGAGGAGTGAAAGGC
  CTCATCATCATCCAGCTCCTCATCGCCATCGAGAAGGCCTCGGGTGTGGCCACCAAGGAC
  CTGTTTGACTGGGTGGCGGGCACCAGCACTGGAGGCATCCTGGCCCTGGCCATTCTGCAC
  AGTAAGTCCATGGCCTACATGCGCGGCATGTACTTTCGCATGAAGGATGAGGTGTTCCGG
  GGCTCCAGGCCCTACGAGTCGGGGCCCCTGGAGGAGTTCCTGAAGCGGGAGTTTGGGGAG
  CACACCAAGATGACGGACGTCAGGAAACCCAAGGTGATGCTGACAGGGACACTGTCTGAC
  CGGCAGCCGGCTGAACTCCACCTCTTCCGGAACTACGATGCTCCAGAAACTGTCCGGGAG
  CCTCGTTTCAACCAGAACGTTAACCTCAGGCCTCCAGCTCAGCCCTCAGACCAGCTGGTG
  TGGCGGGCGGCCCGAAGCAGCGGGGCAGCTCCTACTTACTTCCGACCCAATGGGCGCTTC
  CTGGACGGTGGGCTGCTGGCCAACAACCCCACGCTGGATGCCATGACCGAGATCCATGAG
  TACAATCAGGACCTGATCCGCAAGGGTCAGGCCAACAAGGTGAAGAAACTCTCCATCGTT
  GTCTCCCTGGGGACAGGGAGGTCCCCACAAGTGCCTGTGACCTGTGTGGATGTCTTCCGT
  CCCAGCAACCCCTGGGAGCTGGCCAAGACTGTTTTTGGGGCCAAGGAACTGGGCAAGATG
  GTGGTGGACTGTTGCACGGATCCAGACGGGCGGGCTGTGGACCGGGCACGGGCCTGGTGC
  GAGATGGTCGGCATCCAGTACTTCAGATTGAACCCCCAGCTGGGGACGGACATCATGCTG
  GATGAGGTCAGTGACACAGTGCTGGTCAACGCCCTCTGGGAGACCGAGGTCTACATCTAT
  GAGCACCGCGAGGAGTTCCAGAAGCTCATCCAGCTGCTGCTCTCACCCTGAGGGTCCCCA
  GCCTCTCACCGGCCCCAGCTGACCTCGTCCATTCAGCCCCTGCCAGGCCAAGCCCAGCCA
  CTGCCCTCCCGGGCAGATCTGGGCCCAGGCACCTCTGAGTCCATAGACCAGGCCTGGGAG
  AATGCCAAGCTGCCTGCCCGAGGCTGGTCCTGAAGGCCTGTCTCCCACTAACCCCGCCTT
  CCAGCACTTTCTGTCATTCCAGGCTGGGAAAGTCTAGAGCCCCCTTTGGCCCCTTTCCCT
  GACTGTCAAGGACAACTGACTCCCCCATCAGCTCAAACATTAAGGGTACCCGGGCACAAC
  CGTACCCCTGCCCCCAGCCCCAGCCTCCCTGAGGGCCTGCCGGGCTGCCTCTGCCCCAGC
  CCCCAGCAAGGGCACTCCCAGGCTTCCTGGTGGGTGCAGCCCACTCCCTCTGCCCTCTGC
  TCCGTTCCCTGGGGGCTGGGACTAAAGAAATGGGTGTCCCCCACCCCATCAGCTGGGAAA
  GCCCAGGCCGCAGGAGTGGGATGCCCGTTGGACTTTGCCCCTCACACTGGCCCAGCCCCT
  CACACTGCCCCACCCCGAGAACCCTCAGCTCTCAAAGGTCACTCCTGGGAGTTTCTTCTT
  CCCAATGGAAGTGGCTTAAGAGCCAAAACTGAAATAAATCATTTGGATTCAAAAAAAAAA
  AAAAAAAAAAAAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001004426 unedited
  NGGGGGTTTAGAATTTGTAATACGACTTACTATAGNNGGCGGCCGCGNAATTCCCGGGAT
  GGAAGTAGAAGTGCTGAGTAAGCCGAGACAGAGGGGAAGACGGTGGGGCCTCCCCACCTG
  CCCCGCAGAAGATGCAGTTCTTTGGCCGCCTGGTCAATACCTTCAGTGGCGTCACCAACT
  TGTTCTCTAACCCATTCCGGGTGAAGGAGGTGGCTGTAGCCGACTACACCTCGAGTGACC
  GAGTTCGGGAGGAAGGGCAGCTGATTCTGTTCCAGAACACTCCCAACCGCACCTGGGACT
  GCGTCCTGGTCAACCCCAGGAACTCACAGAGTGGATTCCGACTCTTCCAGCTGGAGTTGG
  AGGCTGACGCCCTAGTGAATTTCCATCAGTATTCTTCCCAGCTGCTACCCTTCTATGAGA
  GCTCCCCTCAGGTCCTGCACACTGAGGTCCTGCAGCACCTGACCGACCTCATCCGTAACC
  ACCCCAGCTGGTCAGTGGCCCACCTGGCTGTGGAGCTAGGGATCCGCGAGTGCTTCCATC
  ACAGCCGTATCATCAGCTGTGCCAATTGCGCGGAGAACGAGGAGGGCTGCACACCCCTGC
  ACCTGGCCTGCCGCAAGGGTGATGGGGAGATCCTGGTGGAGCTGGTGCAGTACTGCCACA
  CTCAGATGGATGTCACCGACTACAAGGGAGAGACCGTCTTCCATTATGCTGTCCAGGGTG
  ACAATTCTCANGTGCTGCAGCNTCTTGGAAGGAACGCAGTGGCTGGCCTGAACCANGTGA
  ATAACCAAGGGCTGACCCCGCTGCACCTGGCCTGCCAGCTGGGGAAGCAAGAGAATGTCC
  GCGTGCTGCTGCTGTGCCATGCTTCGTGCAACATCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001004426
• Insert Size: 3100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001004426.1, NP_001004426.1
• RefSeq Size: 3077 bp
• RefSeq ORF: 2259 bp
• Locus ID: 8398
• Cytogenetics: 22q13.1
• Protein Pathways: alpha-Linolenic acid metabolism, Arachidonic acid metabolism, Ether lipid metabolism, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Glycerophospholipid metabolism, GnRH signaling pathway, Linoleic acid metabolism, Long-term depression, MAPK signaling pathway, Metabolic pathways, Vascular smooth muscle contraction, VEGF signaling pathway
• Gene Summary: The protein encoded by this gene is an A2 phospholipase, a class of enzyme that catalyzes the release of fatty acids from phospholipids. The encoded protein may play a role in phospholipid remodelling, arachidonic acid release, leukotriene and prostaglandin synthesis, fas-mediated apoptosis, and transmembrane ion flux in glucose-stimulated B-cells. Several transcript variants encoding multiple isoforms have been described, but the full-length nature of only three of them have been determined to date. [provided by RefSeq, Dec 2010]
  Transcript Variant: This variant (2) lacks an in-frame exon compared to variant 1. The resulting isoform (b) has the same N- and C-termini but lacks an internal segment compared to isoform a. Variants 2, 3, 5 and 6 encode the same isoform (b). Isoform b is found in the cytoplasm while isoform a is membrane-bound.