EHHADH (BC027486) Human Untagged Clone

CAT#: SC125798

(untagged)-Human cDNA clone MGC:34907 IMAGE:5104096, complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EHHADH
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for BC027486 edited
  GTGGCAGCCCTCGGAATCACGGGGAGGTCACTGCCACAACCTGGCTCTTTGTAGGGGGGT
  CCAACTCCAGGCAGGGGATCACTGCCTGAGGTACCTCTTTAGAGTCCTGAGTGTGGCTCC
  TGGACCATCCCCTTCCCACTGCTTGTCCACGCGCTTGCACCGCTATGAGCGATGACGGCA
  AAGGAAAAGAAAAGCGTCCTCCCTACGAGGTGGTCTGTTTCCCAGTAGCAGGGGGAGCTA
  CGGTACACAAGCACAGGTTCACATTTATGAAGCAGCCAATAAGAATTCCCAGCCTGAGAG
  GACCTTGGAACCAGAAGGAATTGGAGGAGATTTAAACAAGCTGACCCAAGGAAAGCACAA
  GATCTTTCTATGCATTGAAGATCACCAAATCAAAGATAAAAGTGCTCGACCATTATGGCC
  TATGGCAAACTCGGGCTGGCTGAAGCATCCTTCTCTTCCATTTAGAACCCTGGTGAGGCC
  CCTTAGACCACCACTGGGCTGTCCTCCTCCCTAGTCAGAGACAGAGTTCTGGGCTGGGCC
  ATGGAGGTGTCTGTCAACACGGCAGTAGTCCTCGTTCTATGGAAAACCAGCTGTTCCCTG
  TGACACGACTCCATTCCCGCAGCTGCCTGCTATTCAGCTGGGCTGACGGACAGCAGAGCA
  CAGGGTTGGAAGACCACCCTCTCAGACAGGTTTACCAAGAAGCTCACTGAGAGGCGACAC
  AAACGGCCAAGAACATGAAAAGACGGTCAGTGCCCTTGCAGATGGAAGCAGGCAATCATT
  TTTCACCTGCAAGGCTGATAAAGATGAAATGTTCTGATAATACAGGAAGAGTATAAGGAA
  ACTGTCACTCGATGGACTCCAGGTGAAATATAACTGATCCAGCTGCCTGGGAGGGAAAAG
  ATATCTTTCAAAATTGAAATGACAGGAATGGCAGCATTATGGGTGGGAGATAAAGAATCT
  GGGTTTCTTTCAGGGGTGATGAAAATGTTCTAAAACGGATTGTGGTGACAGTTTTCCACA
  ACTCGGAATATACTGAAAACCACCACATTGTATACTTTAAATGGGTGGGTTGTATGGTAT
  GTGAATTATATCTCAATAAAATTTTTACCAAAAGAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC027486 unedited
  NNGGGTCAAAAATTGTATACGACTCACTATAGGCGGCCGCGAATCCCGGGATATCGTCGA
  CCCACGCGTCCGGTGGCAGCCCTCGGAANACGGGGAGGTCACTGCCACAACCTGGCTCTT
  TGTAGGGGGGTCCAACTCCAGGCAGGGGATCACTGCCTGAGGTACCTCTTTAGAGTCCTG
  AGTGTGGCTCCTGGACCATCCCCTTCCCACTGCTTGTCCACGCGCTTGCACCGCTATGAG
  CGATGACGGCAAAGGAAAAGAAAAGCGTCCTCCCTACGAGGTGGTCTGTTTCCCAGTGGC
  AGGGGGAGCTACGGTACACAAGCACAGGTTCACATTTATGAAGCAGCCAATAAGAATTCC
  CAGCCTGAGAGGACCTTGGAACCAGAAGGAATTGGAGGAGATTTAAACAAGCTGACCCAA
  GGAAAGCACAAGATCTTTCTATGCATTGAAGATCACCAAATCAAAGATAAAAGTGCTCGA
  CCATTATGGCCTATGGCAAACTCGGGCTGGCTGAAGCATCCTTCTCTTCCATTTAGAACC
  CTGGTGAGGCCCCTTAGACCACCACTGGGCTGTCCTCCTCCCTCCTCAGAGACAGAGTTC
  TGGGCTGGGCCATGGAGGTGTCTGTCAACACGGCAGTAGTCCTCGTTCTATGGAAAACCA
  GCTGTTCCCTGTGACACGACTCCATTCCCGCAGCTGCCTGCTATTCAGCTGGGCTGACGG
  ACAGCAGAGCACAGGGTTGGAAGACCACCCTCTCAGACAGGTTTACCAAGAAGCTCACTG
  AGAGGCGACACANACGGCCAAGAACATGAAAAGACNGTCAGTGCCCTTGCAGATGGAAGC
  AGGCAATCATTTTTCACCCTGCAGGCTGATAAAGATGAAATGTTCTGATAATACAGGAAG
  AGTATTAGGAAACTGT
  
• Restriction Sites: NotI-NotI
• ACCN: BC027486
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC027486.1
• RefSeq Size: 1130 bp
• Locus ID: 1962
• Cytogenetics: 3q27.2
• Protein Pathways: beta-Alanine metabolism, Butanoate metabolism, Fatty acid metabolism, Limonene and pinene degradation, Lysine degradation, Metabolic pathways, PPAR signaling pathway, Propanoate metabolism, Tryptophan metabolism, Valine, leucine and isoleucine degradation
• Gene Summary: 'The protein encoded by this gene is a bifunctional enzyme and is one of the four enzymes of the peroxisomal beta-oxidation pathway. The N-terminal region of the encoded protein contains enoyl-CoA hydratase activity while the C-terminal region contains 3-hydroxyacyl-CoA dehydrogenase activity. Defects in this gene are a cause of peroxisomal disorders such as Zellweger syndrome. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]'