ATP12A (NM_001676) Human Untagged Clone

CAT#: SC125805

ATP12A (untagged)-Human ATPase, H+/K+ transporting, nongastric, alpha polypeptide (ATP12A), transcript variant 2


  "NM_001676" in other vectors (6)

Reconstitution Protocol

USD 2,110.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol ATP12A
Synonyms ATP1AL1; HK
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for NM_001676 edited
TGGCCGCGGAGGTGCGTGCAGGGCCCGCGCCGCCGCCGGTATCTCCACCGCCAACACCTC
AGCCACTGCCACTGCCACAGCCACACGAGGCCCCCCACCGTGCGCTCCGCCGCTGCGGTC
CCGGATCCGCGCTCCACGCCCGCAGCCCGCGGCGCCACCAGCCCAGCATGCACCAGAAAA
CCCCAGAAATTTACTCCGTGGAGCTCAGCGGAACTAAGGACATCGTGAAAACAGACAAGG
GGGATGGCAAGGAGAAGTATAGGGGTCTGAAGAACAACTGCCTGGAACTCAAAAAGAAAA
ATCACAAAGAGGAGTTTCAGAAAGAACTCCATCTGGATGACCACAAACTCAGCAATAGGG
AATTGGAAGAGAAATATGGCACAGACATCATTATGGGTCTCTCCAGCACCAGAGCTGCCG
AGCTCCTGGCCCGGGATGGGCCCAACTCCCTCACCCCTCCCAAGCAGACGCCTGAGATCG
TCAAGTTCCTCAAGCAGATGGTGGGGGGGTTCTCTATCCTCCTGTGGGTGGGCGCCTTTC
TCTGTTGGATTGCATATGGGATTCAGTACTCCAGCGACAAGTCTGCATCCCTGAACAACG
TGTACTTGGGCTGTGTGCTTGGTCTGGTGGTCATTTTAACGGGGATCTTTGCTTATTACC
AAGAGGCAAAAAGCACCAACATCATGTCCAGCTTCAATAAGATGATCCCTCAGCAAGCTC
TCGTCATCCGAGATTCCGAGAAGAAGACCATCCCTTCAGAGCAGCTGGTGGTGGGGGACA
TTGTGGAGGTCAAAGGAGGAGACCAGATCCCTGCAGACATCAGGGTGCTGTCTTCTCAGG
GGTGTCGGGTGGATAACTCATCTCTCACGGGGGAGTCTGAGCCCCAGCCCCGCTCCTCTG
AGTTTACCCATGAAAACCCCCTGGAAACAAAGAACATCTGCTTCTATTCCACAACGTGTC
TGGAAGCATCTACTTCCCCTGTAGGCACTGTCACCGGCATGGTTATCAACACGGGTGACC
GCACCATCATTGGCCATATTGCCTCATTGGCCTCAGGAGTTGGAAATGAGAAGACGCCCA
TTGCCATTGAGATCGAGCACTTTGTTCACATTGTGGCAGGAGTGGCTGTCTCCATCGGCA
TCCTTTTCTTCATCATCGCTGTGTCCCTGAAGTATCAAGTCCTGGACTCCATCATCTTCC
TCATTGGCATCATTGTGGCCAATGTGCCCGAGGGCCTCCTGGCCACTGTCACTGTGACCC
TGTCGCTGACAGCAAAACGGATGGCCAAGAAGAACTGCCTGGTGAAGAACCTGGAGGCTG
TGGAGACCCTCGGCTCCACCTCCATCATCTGCTCGGACAAGACTGGGACACTGACCCAGA
ACAGGATGACAGTGGCCCATCTGTGGTTCGACAATCAGATCTTTGTGGCTGACACCAGTG
AGGACCATTCAAACCAAGTCTTTGACCAAAGCTCTAGGACTTGGGCCTCCTTATCCAAGA
TAATAACATTGTGTAACCGAGCAGAGTTCAAGCCAGGACAGGAAAATGTCCCCATCATGA
AGAAAGCTGTGATTGGAGATGCCTCAGAAACTGCTCTTTTAAAATTCTCAGAGGTCATTT
TGGGTGATGTGATGGAAATTAGAAAAAGAAACCGCAAAGTAGCTGAAATCCCTTTTAACT
CTACTAATAAATTTCAGCTCTCCATCCACGAGATGGATGACCCCCACGGCAAGCGCTTCC
TCATGGTGATGAAGGGGGCCCCTGAGCGCATTCTAGAGAAATGCAGCACCATCATGATCA
ACGGCGAGGAGCACCCACTGGACAAGAGCACTGCCAAGACCTTCCACACAGCCTACATGG
AGCTGGGCGGGTTGGGCGAGCGTGTGCTGGGTTTCTGTCATCTCTACCTGCCAGCAGACG
AGTTTCCAGAAACCTACTCATTTGACATAGACGCTATGAACTTTCCGACCTCCAACCTCT
GTTTTGTGGGACTCTTGTCAATGATCGATCCCCCTCGGTCCACCGTGCCAGATGCAGTCA
CCAAATGCCGGAGTGCAGGGATCAAGGTTATTATGGTTACTGGTGATCATCCCATCACAG
CCAAAGCTATTGCCAAGAGTGTGGGGATCATTTCAGCCAACAGTGAAACAGTGGAAGACA
TTGCACATCGCCTCAACATTGCTGTGGAGCAAGTTAACAAACGGGATGCCAAGGCCGCTG
TGGTGACTGGCATGGAGCTGAAGGACATGAGCTCAGAACAGCTGGATGAGATCTTAGCCA
ACTACCAGGAGATTGTCTTTGCCCGGACATCCCCCCAGCAGAAGCTGATCATTGTGGAGG
GCTGTCAGAGGCAGGATGCTGTTGTTGCTGTGACCGGGGATGGAGTTAATGACTCTCCGG
CTCTAAAGAAGGCAGACATTGGGATTGCCATGGGGATAGCAGGTTCTGATGCAGCCAAAA
ATGCAGCCGACATGGTCTTGCTGGACGACAACTTCGCATCCATCGTCACAGGGGTGGAGG
AAGGTCGCCTGATCTTTGACAACCTCAAGAAGACTATTGCTTATTCCCTGACCAAGAACA
TTGCCGAGCTGTGCCCCTTTCTGATCTACATCATTGTCGGGCTCCCCCTGCCCATTGGCA
CCATCACCATTCTGTTCATTGACTTGGGGACAGACATTATCCCCTCCATTGCCTTGGCGT
ACGAGAAAGCTGAAAGTGACATCATGAACAGGAAGCCTCGCCACAAGAATAAGGACAGGC
TGGTGAACCAGCCGCTCGCTGTGTACTCATACCTGCACATTGGCCTCATGCAAGCCCTGG
GAGCTTTCCTTGTGTATTTCACCGTCTATGCACAAGAGGGCTTTCTGCCCCGCACTCTCA
TTAACCTGCGGGTAGAATGGGAGAAGGACTACGTGAATGACTTGAAAGACAGCTATGGGC
AGGAATGGACAAGGTACCAGAGGGAATACCTAGAATGGACGGGCTACACGGCTTTCTTTG
TTGGCATCCTAGTCCAGCAAATAGCAGATCTGATCATCAGGAAAACCCGGAGGAATTCCA
TCTTCCAGCAGGGTCTCTTCAGAAATAAAGTCATCTGGGTGGGGATCACCTCACAGATCA
TCATTGGTCTGATCCTCTCCTATGGCCTCGGAAGTGTCACAGCCTTGAGTTTCACCATGC
TTAGGGCTCAGTACTGGTTTGTGGCTGTGCCGCACGCCATCCTGATCTGGGTGTATGATG
AGGTGCGGAAGCTCTTCATCAGGCTCTACCCTGGAAGCTGGTGGGATAAGAACATGTATT
ATTAAGACCACCTCCCTTCCTATGTCTCTCAGCAGCACGTTGGGGCACACTTGTTCATCT
TCTGACCGTTTGCTGGGCTATTCCCCTGCAGTGCAGACATCGTCAAAATTCATACAAGAG
GAAATTTTCATGCAGAAAGCTGTATGCAGGATGCTCACTGATGTTTTGCACTTTAAAACT
GAAATTCAACTCTTTATATAGGATTTTCTTTTCTATCTCCATCTCCTCATTAAAAAATAC
GTACATTTCGAGGTAAAAAAAAAAA
>OriGene 5' read for NM_001676 unedited
CTGGCTACCGGCTCCGGTAATTCCCGGGATCGGCCGCGAGAGTGCGTGCAGGGCCCGCGC
CGCCGCCGGTATCTCCACCGCCAACACCTCAGCCACTGCCACTGCCACAGCCACACGAGG
CCCCCCACCGTGCGCTCCGCCGCTGCGGTCCCGGATCCGCGCTCCACGCCCGCAGCCCGC
GGCGCCACCAGCCCAGCATGCACCAGAAAACCCCAGAAATTTACTCCGTGGAGCTCAGCG
GAACTAAGGACATCGTGAAAACAGACAAGGGGGATGGCAAGGAGAAGTATAGGGGTCTGA
AGAACAACTGCCTGGAACTCAAAAAGAAAAATCACAAAGAGGAGTTTCAGAAAGAACTCC
ATCTGGATGACCACAAACTCAGCAATAGGGAATTGGAAGAGAAATATGGCACAGACATCA
TTATGGGTCTCTCCAGCACCAGAGCTGCCGAGCTCCTGGCCCGGGATGGGCCCAACTCCC
TCACCCCTCCCAAGCAGACGCCTGAGATCGTCAAGTTCCTCAAGCAGATGGTGGGGGGGG
TTCTCTATCCTCCTGTGGGTGGGCGCCTTTCTCTGTTGGATTGCATATGGGATTCAGTAC
TCCAGCGACAAGTCTGCATCCCTGAACAACGTGTACTTGGGCTGTGTGCTTGGTCTGGTG
GTCATTTTAACGGGGATCTTTGCTTATTACCAAGAGGCANAAAGCACCAACATCATGTCC
AGCTTCAAAAGAAGATCCCTCAGCAGCTCTCGTCATCCGAGATCCGAGAAGAGGACATCC
CTTCAAANCAACTGTGGTGGGGGACATGTGGAGGCAAAGGAGAAACAAATCCTGCAGACT
CAGGGTGCTGCCTCCAGGGTGTCGGTGGATACTC
Restriction Sites Please inquire     
ACCN NM_001676
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001676.3, NP_001667.3
RefSeq Size 3594 bp
RefSeq ORF 3138 bp
Locus ID 479
Cytogenetics 13q12.1-q12.3
Protein Families Druggable Genome, Transmembrane
Protein Pathways Oxidative phosphorylation
Gene Summary 'The protein encoded by this gene belongs to the family of P-type cation transport ATPases. This gene encodes a catalytic subunit of the ouabain-sensitive H+/K+ -ATPase that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for potassium absorption in various tissues. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]'
Transcript Variant: This variant (2) uses an alternate in-frame splice junction at the 5' end of an exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1.

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