ATP12A (NM_001676) Human Untagged Clone

CAT#: SC125805

ATP12A (untagged)-Human ATPase, H+/K+ transporting, nongastric, alpha polypeptide (ATP12A), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ATP12A
• Synonyms: ATP1AL1; HK
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001676 edited
  TGGCCGCGGAGGTGCGTGCAGGGCCCGCGCCGCCGCCGGTATCTCCACCGCCAACACCTC
  AGCCACTGCCACTGCCACAGCCACACGAGGCCCCCCACCGTGCGCTCCGCCGCTGCGGTC
  CCGGATCCGCGCTCCACGCCCGCAGCCCGCGGCGCCACCAGCCCAGCATGCACCAGAAAA
  CCCCAGAAATTTACTCCGTGGAGCTCAGCGGAACTAAGGACATCGTGAAAACAGACAAGG
  GGGATGGCAAGGAGAAGTATAGGGGTCTGAAGAACAACTGCCTGGAACTCAAAAAGAAAA
  ATCACAAAGAGGAGTTTCAGAAAGAACTCCATCTGGATGACCACAAACTCAGCAATAGGG
  AATTGGAAGAGAAATATGGCACAGACATCATTATGGGTCTCTCCAGCACCAGAGCTGCCG
  AGCTCCTGGCCCGGGATGGGCCCAACTCCCTCACCCCTCCCAAGCAGACGCCTGAGATCG
  TCAAGTTCCTCAAGCAGATGGTGGGGGGGTTCTCTATCCTCCTGTGGGTGGGCGCCTTTC
  TCTGTTGGATTGCATATGGGATTCAGTACTCCAGCGACAAGTCTGCATCCCTGAACAACG
  TGTACTTGGGCTGTGTGCTTGGTCTGGTGGTCATTTTAACGGGGATCTTTGCTTATTACC
  AAGAGGCAAAAAGCACCAACATCATGTCCAGCTTCAATAAGATGATCCCTCAGCAAGCTC
  TCGTCATCCGAGATTCCGAGAAGAAGACCATCCCTTCAGAGCAGCTGGTGGTGGGGGACA
  TTGTGGAGGTCAAAGGAGGAGACCAGATCCCTGCAGACATCAGGGTGCTGTCTTCTCAGG
  GGTGTCGGGTGGATAACTCATCTCTCACGGGGGAGTCTGAGCCCCAGCCCCGCTCCTCTG
  AGTTTACCCATGAAAACCCCCTGGAAACAAAGAACATCTGCTTCTATTCCACAACGTGTC
  TGGAAGCATCTACTTCCCCTGTAGGCACTGTCACCGGCATGGTTATCAACACGGGTGACC
  GCACCATCATTGGCCATATTGCCTCATTGGCCTCAGGAGTTGGAAATGAGAAGACGCCCA
  TTGCCATTGAGATCGAGCACTTTGTTCACATTGTGGCAGGAGTGGCTGTCTCCATCGGCA
  TCCTTTTCTTCATCATCGCTGTGTCCCTGAAGTATCAAGTCCTGGACTCCATCATCTTCC
  TCATTGGCATCATTGTGGCCAATGTGCCCGAGGGCCTCCTGGCCACTGTCACTGTGACCC
  TGTCGCTGACAGCAAAACGGATGGCCAAGAAGAACTGCCTGGTGAAGAACCTGGAGGCTG
  TGGAGACCCTCGGCTCCACCTCCATCATCTGCTCGGACAAGACTGGGACACTGACCCAGA
  ACAGGATGACAGTGGCCCATCTGTGGTTCGACAATCAGATCTTTGTGGCTGACACCAGTG
  AGGACCATTCAAACCAAGTCTTTGACCAAAGCTCTAGGACTTGGGCCTCCTTATCCAAGA
  TAATAACATTGTGTAACCGAGCAGAGTTCAAGCCAGGACAGGAAAATGTCCCCATCATGA
  AGAAAGCTGTGATTGGAGATGCCTCAGAAACTGCTCTTTTAAAATTCTCAGAGGTCATTT
  TGGGTGATGTGATGGAAATTAGAAAAAGAAACCGCAAAGTAGCTGAAATCCCTTTTAACT
  CTACTAATAAATTTCAGCTCTCCATCCACGAGATGGATGACCCCCACGGCAAGCGCTTCC
  TCATGGTGATGAAGGGGGCCCCTGAGCGCATTCTAGAGAAATGCAGCACCATCATGATCA
  ACGGCGAGGAGCACCCACTGGACAAGAGCACTGCCAAGACCTTCCACACAGCCTACATGG
  AGCTGGGCGGGTTGGGCGAGCGTGTGCTGGGTTTCTGTCATCTCTACCTGCCAGCAGACG
  AGTTTCCAGAAACCTACTCATTTGACATAGACGCTATGAACTTTCCGACCTCCAACCTCT
  GTTTTGTGGGACTCTTGTCAATGATCGATCCCCCTCGGTCCACCGTGCCAGATGCAGTCA
  CCAAATGCCGGAGTGCAGGGATCAAGGTTATTATGGTTACTGGTGATCATCCCATCACAG
  CCAAAGCTATTGCCAAGAGTGTGGGGATCATTTCAGCCAACAGTGAAACAGTGGAAGACA
  TTGCACATCGCCTCAACATTGCTGTGGAGCAAGTTAACAAACGGGATGCCAAGGCCGCTG
  TGGTGACTGGCATGGAGCTGAAGGACATGAGCTCAGAACAGCTGGATGAGATCTTAGCCA
  ACTACCAGGAGATTGTCTTTGCCCGGACATCCCCCCAGCAGAAGCTGATCATTGTGGAGG
  GCTGTCAGAGGCAGGATGCTGTTGTTGCTGTGACCGGGGATGGAGTTAATGACTCTCCGG
  CTCTAAAGAAGGCAGACATTGGGATTGCCATGGGGATAGCAGGTTCTGATGCAGCCAAAA
  ATGCAGCCGACATGGTCTTGCTGGACGACAACTTCGCATCCATCGTCACAGGGGTGGAGG
  AAGGTCGCCTGATCTTTGACAACCTCAAGAAGACTATTGCTTATTCCCTGACCAAGAACA
  TTGCCGAGCTGTGCCCCTTTCTGATCTACATCATTGTCGGGCTCCCCCTGCCCATTGGCA
  CCATCACCATTCTGTTCATTGACTTGGGGACAGACATTATCCCCTCCATTGCCTTGGCGT
  ACGAGAAAGCTGAAAGTGACATCATGAACAGGAAGCCTCGCCACAAGAATAAGGACAGGC
  TGGTGAACCAGCCGCTCGCTGTGTACTCATACCTGCACATTGGCCTCATGCAAGCCCTGG
  GAGCTTTCCTTGTGTATTTCACCGTCTATGCACAAGAGGGCTTTCTGCCCCGCACTCTCA
  TTAACCTGCGGGTAGAATGGGAGAAGGACTACGTGAATGACTTGAAAGACAGCTATGGGC
  AGGAATGGACAAGGTACCAGAGGGAATACCTAGAATGGACGGGCTACACGGCTTTCTTTG
  TTGGCATCCTAGTCCAGCAAATAGCAGATCTGATCATCAGGAAAACCCGGAGGAATTCCA
  TCTTCCAGCAGGGTCTCTTCAGAAATAAAGTCATCTGGGTGGGGATCACCTCACAGATCA
  TCATTGGTCTGATCCTCTCCTATGGCCTCGGAAGTGTCACAGCCTTGAGTTTCACCATGC
  TTAGGGCTCAGTACTGGTTTGTGGCTGTGCCGCACGCCATCCTGATCTGGGTGTATGATG
  AGGTGCGGAAGCTCTTCATCAGGCTCTACCCTGGAAGCTGGTGGGATAAGAACATGTATT
  ATTAAGACCACCTCCCTTCCTATGTCTCTCAGCAGCACGTTGGGGCACACTTGTTCATCT
  TCTGACCGTTTGCTGGGCTATTCCCCTGCAGTGCAGACATCGTCAAAATTCATACAAGAG
  GAAATTTTCATGCAGAAAGCTGTATGCAGGATGCTCACTGATGTTTTGCACTTTAAAACT
  GAAATTCAACTCTTTATATAGGATTTTCTTTTCTATCTCCATCTCCTCATTAAAAAATAC
  GTACATTTCGAGGTAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001676 unedited
  CTGGCTACCGGCTCCGGTAATTCCCGGGATCGGCCGCGAGAGTGCGTGCAGGGCCCGCGC
  CGCCGCCGGTATCTCCACCGCCAACACCTCAGCCACTGCCACTGCCACAGCCACACGAGG
  CCCCCCACCGTGCGCTCCGCCGCTGCGGTCCCGGATCCGCGCTCCACGCCCGCAGCCCGC
  GGCGCCACCAGCCCAGCATGCACCAGAAAACCCCAGAAATTTACTCCGTGGAGCTCAGCG
  GAACTAAGGACATCGTGAAAACAGACAAGGGGGATGGCAAGGAGAAGTATAGGGGTCTGA
  AGAACAACTGCCTGGAACTCAAAAAGAAAAATCACAAAGAGGAGTTTCAGAAAGAACTCC
  ATCTGGATGACCACAAACTCAGCAATAGGGAATTGGAAGAGAAATATGGCACAGACATCA
  TTATGGGTCTCTCCAGCACCAGAGCTGCCGAGCTCCTGGCCCGGGATGGGCCCAACTCCC
  TCACCCCTCCCAAGCAGACGCCTGAGATCGTCAAGTTCCTCAAGCAGATGGTGGGGGGGG
  TTCTCTATCCTCCTGTGGGTGGGCGCCTTTCTCTGTTGGATTGCATATGGGATTCAGTAC
  TCCAGCGACAAGTCTGCATCCCTGAACAACGTGTACTTGGGCTGTGTGCTTGGTCTGGTG
  GTCATTTTAACGGGGATCTTTGCTTATTACCAAGAGGCANAAAGCACCAACATCATGTCC
  AGCTTCAAAAGAAGATCCCTCAGCAGCTCTCGTCATCCGAGATCCGAGAAGAGGACATCC
  CTTCAAANCAACTGTGGTGGGGGACATGTGGAGGCAAAGGAGAAACAAATCCTGCAGACT
  CAGGGTGCTGCCTCCAGGGTGTCGGTGGATACTC
  
• Restriction Sites: Please inquire
• ACCN: NM_001676
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001676.3, NP_001667.3
• RefSeq Size: 3594 bp
• RefSeq ORF: 3138 bp
• Locus ID: 479
• Cytogenetics: 13q12.1-q12.3
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Oxidative phosphorylation
• Gene Summary: 'The protein encoded by this gene belongs to the family of P-type cation transport ATPases. This gene encodes a catalytic subunit of the ouabain-sensitive H+/K+ -ATPase that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for potassium absorption in various tissues. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]'
  Transcript Variant: This variant (2) uses an alternate in-frame splice junction at the 5' end of an exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1.