CPSF30 (CPSF4) (BC050738) Human Untagged Clone

CAT#: SC125853

CPSF4 (untagged)-Human cleavage and polyadenylation specific factor 4, 30kDa, mRNA (cDNA clone MGC:60384 IMAGE:6199123), complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CPSF4
• Synonyms: cleavage-polyadenylation specificity factor, 30kD; cleavage and polyadenylation specific factor 4, 30kDa; CPSF30; NAR; NEB1; no arches-like zinc finger protein
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for BC050738 edited
  GGCGAAGCGAAGGAGGAGTGTGTGCGGCGGGGCCGGCGGCGGGTAAAGGCGAGAAGGCTG
  CAGGAGACCGAGGGGGAGCCGGGCCGGTGGGGCCGCCGCCGCCGCCATGCAGGAAATCAT
  CGCCAGCGTGGACCACATCAAGTTTGACTTGGAGATCGCGGTGGAGCAGCAGCTGGGGGC
  GCAGCCGCTGCCCTTCCCCGGCATGGACAAGTCGGGCGCTGCTGTCTGTGAATTCTTTTT
  GAAAGCTGCCTGCGGCAAAGGGGGCATGTGTCCGTTTCGCCACATCAGTGGTGAGAAGAC
  AGTTGTGTGCAAACACTGGCTGCGTGGCCTATGCAAGAAAGGGGACCAGTGTGAGTTCCT
  GCATGAGTATGACATGACCAAGATGCCCGAGTGCTACTTCTACTCCAAGTTCGGGGAGTG
  CAGCAACAAGGAATGTCCCTTCCTGCACATCGACCCCGAGTCCAAGATCAAGGACTGTCC
  TTGGTATGACCGCGGCTTCTGCAAGCACGGTCCCCTCTGCAGGCACCGGCACACACGGAG
  AGTCATCTGTGTGAATTACCTCGTGGGATTCTGCCCGGAGGGGCCCTCGTGTAAATTCAT
  GCACCCTCGATTTGAACTGCCCATGGGAACCACCGAGCAGCCCCCACTGCCACAGCAGAC
  ACAGCCTCCAGCAAAGAGAACCCCGCAGGTCATCGGGGTCATGCAGAGTCAAAACAGCAG
  CGCGGGCAACCGGGGACCCCGGCCACTGGAGCAGGTCACCTGTTACAAGTGTGGCGAGAA
  AGGACACTACGCCAACAGATGCACCAAAGGGCACTTGGCCTTTCTCAGTGGACAGTGACA
  GCAGCTGGAGCCAGCTCCGAGCAGCCCGGGGGCCCCGCTGTTGGGAGTGTGCATTTAACT
  GTTTCATGCGCTTGTTGGCGCGACTGTGGCTCGAGCTGGCCCGCAGACACGTGGGTTTCA
  TCACTCTGAGGGGCCACGTCTGTTAGTTTCCTATCATTTTGCCGTAGTATTTTTTGAAAA
  AGGGACATGTGTCCTGTGGGTCCCTGCAGTCGACATCATGTTTGGCTGGGCATCGATGCC
  TCCTTTCTGGGACTCCCGGCACAACTCCCTGCCCTGCTGAATCCTAAAGCTGTGCCTATA
  TCTGTGATTTGAATGAGGGAGCCCTTTGGGGCAAATTCAGGTGCCCCCATTGCCTCAGGC
  TGGCCCTGGTCCCAGGTGGCAGCGGTTGAGGAGGGGTACAGGGCTCTCAAGCCTGAGGTT
  TTCTTCTCTGGGCTTAATTTTCTCTTGGGGTACGTGCCTGACAGTGTTTAAGGTGTCCGT
  TGAACTGGAGTTGCAGACTTTTAAATAGATGACCCCTTCAGATCATCTGTGCCTACCTCC
  TGCCCATCAGGCGTCTACACTGTCACTCAGACACCTGTGGCATGTGGAGGAGACTGCCCT
  GTCCTGAGCCTGGAAAATGTGAAACTGTCTCCTGCAACCTGCTGGGCATGTGGGCCTGGC
  TGTGTTCAATTGCAAGAACAATTTTTATGAAATGGATTAAAGCTTGTTTTTTAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC050738 unedited
  NGGAGTTTGAAATTTGTATACGACTCATATAGGCGGCCGCGNATTCAGATCTGGTACCGG
  TCCGGAATTCCCGGGATATCGTCGACCCACGCGTCCGGGCGAAGCGAAGGAGGAGTGTGT
  GCGGCGGGGCCGGCGGCGGGTAAAGGCGAGAAGGCTGCAGGAGACCGAGGGGGAGCCGGG
  CCGGTGGGGCCGCCGCCGCCGCCATGCAGGAAATCATCGCCAGCGTGGACCACATCAAGT
  TTGACTTGGAGATCGCGGTGGAGCAGCAGGTGGGGGCGCAGCCGCTGCCCTTCCCCGGCA
  TGGACAAGTCGGGCGCTGCTGTCTGTGAATTCTTTTTGAAAGCTGCCTGCGGCAAAGGGG
  GCATGTGTCCGTTTCGCCACATCAGTGGTGAGAAGACAGTTGTGTGCAAACACTGGCTGC
  GTGGCCTATGCAAGAAAGGGGACCAGTGTGAGTTCCTGCATGAGTATGACATGACCAAGA
  TGCCCGAGTGCTACTTCTACTCCAAGTTCGGGGAGTGCAGCAACAAGGAATGTCCCTTCC
  TGCACATCGACCCCGAGTCCAAGATCAAGGACTGTCCTTGGTATGACCGCGGCTTCTGCA
  AGCACGGTCCCCTCTGCAGGCACCGGCACACACGGAGAGTCATCTGTGTGAATTACCTCG
  TGGGATTCTGCCCGGAGGGGCCCTCGTGTAAATTCATGCACCCTCGATTTGAACTGCCCA
  TGGGAACCACCGAGCAGCCCCCACTGCCACAGCAGACACAGCCTCCAGCAAAGAGAACCC
  CGCCAGTCATCGGGGTCATGCAGAGTCAAACAGCAGCGCGGGCAACCGGGGACCCCGGCC
  ACTGGAGCAGGTCACCTGTTACAAGTGTGGC
  
• Restriction Sites: NotI-NotI
• ACCN: BC050738
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info

Reference Data

• RefSeq: BC050738.1, AAH50738.1
• RefSeq Size: 1640
• Locus ID: 10898
• Gene Summary: Inhibition of the nuclear export of poly(A)-containing mRNAs caused by the influenza A virus NS1 protein requires its effector domain. The NS1 effector domain functionally interacts with the cellular 30 kDa subunit of cleavage and polyadenylation specific factor 4, an essential component of the 3' end processing machinery of cellular pre-mRNAs. In influenza virus-infected cells, the NS1 protein is physically associated with cleavage and polyadenylation specific factor 4, 30kD subunit. Binding of the NS1 protein to the 30 kDa protein in vitro prevents CPSF binding to the RNA substrate and inhibits 3' end cleavage and polyadenylation of host pre-mRNAs. Thus the NS1 protein selectively inhibits the nuclear export of cellular, and not viral, mRNAs. Multiple alternatively spliced transcript variants that encode different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]