Kv1.2 (KCNA2) (BC043564) Human Untagged Clone

CAT#: SC125885

KCNA2 (untagged)-Human potassium voltage-gated channel, shaker-related subfamily, member 2, mRNA (cDNA clone MGC:50217 IMAGE:5212949), complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KCNA2
• Synonyms: HBK5; HK4; HUKIV; KV1.2; MGC50217; MK2; NGK1; potassium channel; potassium voltage-gated channel, shaker-related subfamily, member 2; RBK2; voltage-gated potassium channel protein Kv1.2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for BC043564 edited
  GGCACATAGAAGAGTGAGCCATCATCTGGTTTCCAGCGCCAAGACTTCTGCAGGAGAGCC
  TCCGCTGGGCTTTCAAGTTGGCAACGTCACACCTCCTGAGGACAGCCAGGACTCCAGCTT
  TTGCTGAGCTTTGCATCTTGCCTCCTTCCTTCAAAAGGCTCCAGGCCCATGCTCGGCTGT
  CTTCAAGTCCCGCATGCCCCTGGCCAGGCACCTCACCGCCCCTCCAGCCCAGCCCAATCC
  TAGAGCTCACCTCCAGGACCCAGAGTCTGCCCTTCTGCCCAGAATGACTCACCATTATTT
  CTAGCTCGAGTGAGAAGACGTGATGAGGAGTTGGGCTGCCTGTTGGTGCATCTCAGATTC
  CTGCAGCATCTGATCAGCTCTGCAAGGAAGAGAGCTTCACGTCTCTGGTCAGCCAAGCGA
  GGCTGTCTCTCCAGCTCTCAGAGAGCTCTCGGGGCTCTCCTGCAGGAGACCAGGCCAATG
  CTCCTGTGCTTCCTGGGGCCAGTAGCAGCACCCTGAGCTCCCTGCCACCAGGCAGCTGAA
  AGGCATAGCGTGAGGTGCTTCTCTCAGTCCCAATTATGACAGTGGCCACCGGAGACCCAG
  CAGACGAGGCTGCTGCCCTCCCTGGGCACCCACAGGACACCTATGACCCAGAGGCAGACC
  ACGAGTGCTGTGAGAGGGTGGTGATCAACATCTCAGGGCTGCGGTTTGAGACCCAGCTAA
  AGACCTTAGCCCAGTTTCCAGAGACCCTCTTAGGGGACCCAAAGAAACGAATGAGGTACT
  TTGACCCCCTCCGGAATGAGTACTTTTTCGATCGGAACCGCCCTAGCTTTGATGCCATTT
  TGTACTACTACCAGTCAGGGGGCCGATTGAGGCGACCTGTGAATGTGCCCTTAGATATAT
  TCTCTGAAGAAATTCGGTTTTATGAGCTGGGAGAAGAAGCGATGGAGATGTTTCGGGAAG
  ATGAAGGCTACATCAAGGAGGAAGAGCGTCCTCTGCCTGAAAATGAGTTTCAGAGACAAG
  TGTGGCTTCTCTTTGAATACCCAGAGAGCTCAGGGCCTGCCAGGATTATAGCTATTGTGT
  CTGTCATGGTGATTCTGATCTCAATTGTCAGCTTCTGTCTGGAAACATTGCCCATCTTCC
  GGGATGAGAATGAAGACATGCATGGTAGTGGGGTGACCTTCCACACCTATTCCAACAGCA
  CCATCGGGTACCAGCAGTCCACTTCCTTCACAGACCCTTTCTTCATTGTAGAGACACTCT
  GCATCATCTGGTTCTCCTTTGAATTCTTGGTGAGGTTCTTTGCCTGTCCCAGCAAAGCCG
  GCTTCTTCACCAACATCATGAACATCATTGACATTGTGGCCATCATCCCCTACTTCATCA
  CCCTGGGGACAGAGTTGGCTGAGAAGCCAGAGGACGCTCAGCAAGGCCAGCAGGCCATGT
  CACTGGCCATCCTCCGTGTCATCCGGTTGGAACGCAGACCTCTGCAAAGCCAGAAGAGTA
  AGCGGGGAAGGCAGCATCTGAACACCTCACATGACTGCACCTTAGGAATTAACCTAGTCG
  CGGGCATGACTGTACAGTGGACCAGGGCATCTGGTCCTGATGACAGGCAGACACCAGCTG
  TAACTACATTGCACAGGATGTATTGATATTATCATTCTTGCTGACATATTCTGGAAGCTT
  TCAGCCCCACCAACACTGCCCCACCCAGGACTTCTGCAGCTGAGAGTTCCTGTGTCAGTA
  GGGTCTGATATTTGTACATAGGTTATACATATATGTGTACATATGTGCCTCAATGAACAT
  TGCCTGTCCACCTGATCATGGGTGTACATAGACTTCATAGAGCTCCACAGTCTTTTGTAA
  ATATTGACACAAGTAAATAAGTATATAAATATATATTACTGGGGATTTATTTTAACATCA
  TTCTGTGTGATATGAAAAAAATAAAATTTTTGACAGACAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC043564 unedited
  NGGCGTTCAAATTTGTATACGACTCACTATGGGCGGCCGCGAATCAAATCTGGTACCGGT
  CCGGAATTCCCGGGATGGCACATAGAAGAGTGAGCCATCATCTGGTTTCCAGCGCCAAGA
  CTTCTGCAGGAGAGCCTCCGCTGGGCTTTCAAGTTGGCAACGTCACACCTCCTGAGGACA
  GCCAGGACTCCAGCTTTTGCTGAGCTTTGCATCTTGCCTCCTTCCTTCAAAAGGCTCCAG
  GCCCATGCTCGGCTGTCTTCAAGTCCCGCATGCCCCTGGCCAGGCACCTCACCGCCCCTC
  CAGCCCAGCCCAATCCTAGAGCTCACCTCCAGGACCCAGAGTCTGCCCTTCTGCCCAGAA
  TGACTCACCATTATTTCTAGCTCGAGTGAGAAGACGTGATGAGGAGTTGGGCTGCCTGTT
  GGTGCATCTCAGATTCCTGCAGCATCTGATCAGCTCTGCAAGGAAGAGAGCTTCACGTCT
  CTGGTCAGCCAAGCGAGGCTGTCTCTCCAGCTCTCAGAGAGCTCTCGGGGCTCTCCTGCA
  GGAGACCAGGCCAATGCTCCTGTGCTTCCTGGGGCCAGTAGCAGCACCCTGAGCTCCCTG
  CCACCAGGCAGCTGAAAGGCATAGCGTGAGGTGCTTCTCTCAGTCCCAATTATGACAGTG
  GCCACCGGAGACCCAGCAGACGAGGCTGCTGCCCTCCCTGGGCACCCACAGGACACCTAT
  GACCCAGAGGCAGACCACGAGTGCTGTGAGAGGGTGGTGATCAACATCTCAGGGCTGCNG
  TTTGAGACCCAGCTAAAGACTTAGNCCCATTTCCAGAGACCCTCTTTAGGGACCCAAGAA
  ACGAATGGAGTACTTTGACCCCCTCCGAATGA
  
• Restriction Sites: NotI-NotI
• ACCN: BC043564
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC043564.1, AAH43564.1
• RefSeq Size: 2022 bp
• RefSeq ORF: 1068 bp
• Locus ID: 3737
• Cytogenetics: 1p13.3
• Protein Families: Druggable Genome, Ion Channels: Potassium, Transmembrane
• Gene Summary: 'Potassium channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. Four sequence-related potassium channel genes - shaker, shaw, shab, and shal - have been identified in Drosophila, and each has been shown to have human homolog(s). This gene encodes a member of the potassium channel, voltage-gated, shaker-related subfamily. This member contains six membrane-spanning domains with a shaker-type repeat in the fourth segment. It belongs to the delayed rectifier class, members of which allow nerve cells to efficiently repolarize following an action potential. The coding region of this gene is intronless, and the gene is clustered with genes KCNA3 and KCNA10 on chromosome 1. [provided by RefSeq, Jul 2008]'