MYOD1 (NM_002478) Human Untagged Clone

CAT#: SC125952

MYOD1 (untagged)-Human myogenic differentiation 1 (MYOD1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MYOD1
• Synonyms: bHLHc1; MYF3; MYOD; PUM
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002478 edited
  CACTTTGCTATCTACAGCTTGGGTTGGGCGAAGCCAGGACCGTGCCGCGCCACCGCCAGG
  ATATGGAGCTACTGTCGCCACCGCTCCGCGACGTAGACCTGACGGCCCCCGACGGCTCTC
  TCTGCTCCTTTGCCACAACGGACGACTTCTATGACGACCCGTGTTTCGACTCCCCGGACC
  TGCGCTTCTTCGAAGACCTGGACCCGCGCCTGATGCACGTGGGCGCGCTCCTGAAACCCG
  AAGAGCACTCGCACTTCCCCGCGGCGGTGCACCCGGCCCCGGGCGCACGTGAGGACGAGC
  ATGTGCGCGCGCCCAGCGGGCACCACCAGGCGGGCCGCTGCCTACTGTGGGCCTGCAAGG
  CGTGCAAGCGCAAGACCACCAACGCCGACCGCCGCAAGGCCGCCACCATGCGCGAGCGGC
  GCCGCCTGAGCAAAGTAAATGAGGCCTTTGAGACACTCAAGCGCTGCACGTCGAGCAATC
  CAAACCAGCGGTTGCCCAAGGTGGAGATCCTGCGCAACGCCATCCGCTATATCGAGGGCC
  TGCAGGCTCTGCTGCGCGACCAGGACGCCGCGCCCCCTGGCGCCGCAGCCGCCTTCTATG
  CGCCGGGCCCGCTGCCCCCGGGCCGCGGCGGCGAGCACTACAGCGGCGACTCCGACGCGT
  CCAGCCCGCGCTCCAACTGCTCCGACGGCATGATGGACTACAGCGGCCCCCCGAGCGGCG
  CCCGGCGGCGGAACTGCTACGAAGGCGCCTACTACAACGAGGCGCCCAGCGAACCCAGGC
  CCGGGAAGAGTGCGGCGGTGTCGAGCCTAGACTGCCTGTCCAGCATCGTGGAGCGCATCT
  CCACCGAGAGCCCTGCGGCGCCCGCCCTCCTGCTGGCGGACGTGCCTTCTGAGTCGCCTC
  CGCGCAGGCAAGAGGCTGCCGCCCCCAGCGAGGGAGAGAGCAGCGGCGACCCCACCCAGT
  CACCGGACGCCGCCCCGCAGTGCCCTGCGGGTGCGAACCCCAACCCGATATACCAGGTGC
  TCTGAGGGGATGGTGGCCGCCCACCCGCCCGAGGGATGGTGCCCCTAGGGTCCCTCGCGC
  CCAAAAGATTGAACTTAAATGCCCCCCTCCCAACAGCGCTTTAAAAGCGACCTCTCTTGA
  GGTAGGAGAGGCGGGAGAACTGAAGTTTCCGCCCCCGCCCCACAGGGCAAGGACACAGCG
  CGGTTTTTTCCACGCAGCACCCTTCTCGGAGACCCATTGCGATGGCCGCTCCGTGTTCCT
  CGGTGGGCCAGAGCTGAACCTTGAGGGGCTAGGTTCAGCTTTCTCGCGCCCTCCCCCATG
  GGGGTGAGACCCTCGCAGACCTAAGCCCTGCCCCGGGATGCACCGGTTATTTGGGGGGGC
  GTGAGACCCAGTGCACTCCGGTCCCAAATGTAGCAGGTGTAACCGTAACCCACCCCCAAC
  CCGTTTCCCGGTTCAGGACCACTTTTTGTAATACTTTTGTAATCTATTCCTGTAAATAAG
  AGTTGCTTTGCCAGAGCAGGAGCCCCTGGGGCTGTATTTATCTCTGAGGCATGGTGTGTG
  GTGCTACAGGGAATTTGTACGTTTATACCGCAGGCGGGCGAGCCGCGGGCGCTCGCTCAG
  GTGATCAAAATAAAGGCGCTAATTTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002478 unedited
  NCCCCGTCGNCATTTGTATACGACTCATATAGGCGGACCGCGAATTCGCACGAGGCACTT
  TGCTATCTACAGCTTGGGTTGGGCGAAGCCAGGACCGTGCCGCGCCACCGCCAGGATATG
  GAGCTACTGTCGCCACCGCTCCGCGACGTAGACCTGACGGCCCCCGACGGCTCTCTCTGC
  TCCTTTGCCACAACGGACGACTTCTATGACGACCCGTGTTTCGACTCCCCGGACCTGCGC
  TTCTTCGAAGACCTGGACCCGCGCCTGATGCACGTGGGCGCGCTCCTGAAACCCGAAGAG
  CACTCGCACTTCCCCGCGGCGGTGCACCCGGCCCCGGGCGCACGTGAGGACGAGCATGTG
  CGCGCGCCCAGCGGGCACCACCAGGCGGGCCGCTGCCTACTGTGGGCCTGCAAGGCGTGC
  AAGCGCAAGACCACCAACGCCGACCGCCGCAAGGCCGCCACCATGCGCGAGCGGCGCCGC
  CTGAGCAAAGTAAATGAGGCCTTTGAGACACTCAAGCGCTGCACGTCGAGCAATCCAAAC
  CAGCGGTTGCCCAAGGTGGAGATCCTGCGCAACGCCATCCGCTATATCGAGGGCCTGCAG
  GCTCTGCTGCGCGACCAGGACGCCGCGCCCCCTGGCGCCGCAGCCGCCTTCTATGCGCCG
  GGCCCGCTGCCCCCGGGCCGCGGCGGCGAGCACTACAGCGGCGACTCCGACGCGTCCAGC
  CCGCGCTCCAACTGCTCCGACGGCATGATGGACTACAGCGGCCCCCCGAGCGGCGCCCGG
  CGGCGGAACTGCTACGAAAGCGCCTACTACAACGAGGCGCCCAGCGAACCCAGGCCCGGG
  AAGAGTGCNGCGGTGTCNAGCCTAGACTGGCTGTCCAGCATCGTGGAACGCATCTCCACC
  GA
  
• Restriction Sites: Please inquire
• ACCN: NM_002478
• Insert Size: 1800 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002478.3, NP_002469.2
• RefSeq Size: 1757 bp
• RefSeq ORF: 963 bp
• Locus ID: 4654
• Cytogenetics: 11p15.1
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: 'This gene encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis. [provided by RefSeq, Jul 2008]'