CEACAM5 (NM_004363) Human Untagged Clone

CAT#: SC126003

CEACAM5 (untagged)-Human carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CEACAM5
• Synonyms: CD66e; CEA
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004363 edited
  GGACAGCAGACCAGACAGTCACAGCAGCCTTGACAAAACGTTCCTGGAACTCAAGCTCTT
  CTCCACAGAGGAGGACAGAGCAGACAGCAGAGACCATGGAGTCTCCCTCGGCCCCTCCCC
  ACAGATGGTGCATCCCCTGGCAGAGGCTCCTGCTCACAGCCTCACTTCTAACCTTCTGGA
  ACCCGCCCACCACTGCCAAGCTCACTATTGAATCCACGCCGTTCAATGTCGCAGAGGGGA
  AGGAGGTGCTTCTACTTGTCCACAATCTGCCCCAGCATCTTTTTGGCTACAGCTGGTACA
  AAGGTGAAAGAGTGGATGGCAACCGTCAAATTATAGGATATGTAATAGGAACTCAACAAG
  CTACCCCAGGGCCCGCATACAGTGGTCGAGAGATAATATACCCCAATGCATCCCTGCTGA
  TCCAGAACATCATCCAGAATGACACAGGATTCTACACCCTACACGTCATAAAGTCAGATC
  TTGTGAATGAAGAAGCAACTGGCCAGTTCCGGGTATACCCGGAGCTGCCCAAGCCCTCCA
  TCTCCAGCAACAACTCCAAACCCGTGGAGGACAAGGATGCTGTGGCCTTCACCTGTGAAC
  CTGAGACTCAGGACGCAACCTACCTGTGGTGGGTAAACAATCAGAGCCTCCCGGTCAGTC
  CCAGGCTGCAGCTGTCCAATGGCAACAGGACCCTCACTCTATTCAATGTCACAAGAAATG
  ACTCAGCAAGCTACAAATGTGAAACCCAGAACCCAGTGAGTGCCAGGCGCAGTGATTCAG
  TCATCCTGAATGTCCTCTATGGCCCGGATGCCCCCACCATTTCCCCTCTAAACACATCTT
  ACAGATCAGGGGAAAATCTGAACCTCTCCTGCCACGCAGCCTCTAACCCACCTGCACAGT
  ACTCTTGGTTTGTCAATGGGACTTTCCAGCAATCCACCCAAGAGCTCTTTATCCCCAACA
  TCACTGTGAATAATAGTGGATCCTATACGTGCCAAGCCCATAACTCAGACACTGGCCTCA
  ATAGGACCACAGTCACGACGATCACAGTCTATGCAGAGCCACCCAAACCCTTCATCACCA
  GCAACAACTCCAACCCCGTGGAGGATGAGGATGCTGTAGCCTTAACCTGTGAACCTGAGA
  TTCAGAACACAACCTACCTGTGGTGGGTAAATAATCAGAGCCTCCCGGTCAGTCCCAGGC
  TGCAGCTGTCCAATGACAACAGGACCCTCACTCTACTCAGTGTCACAAGGAATGATGTAG
  GACCCTATGAGTGTGGAATCCAGAACGAATTAAGTGTTGACCACAGCGACCCAGTCATCC
  TGAATGTCCTCTATGGCCCAGACGACCCCACCATTTCCCCCTCATACACCTATTACCGTC
  CAGGGGTGAACCTCAGCCTCTCCTGCCATGCAGCCTCTAACCCACCTGCACAGTATTCTT
  GGCTGATTGATGGGAACATCCAGCAACACACACAAGAGCTCTTTATCTCCAACATCACTG
  AGAAGAACAGCGGACTCTATACCTGCCAGGCCAATAACTCAGCCAGTGGCCACAGCAGGA
  CTACAGTCAAGACAATCACAGTCTCTGCGGAGCTGCCCAAGCCCTCCATCTCCAGCAACA
  ACTCCAAACCCGTGGAGGACAAGGATGCTGTGGCCTTCACCTGTGAACCTGAGGCTCAGA
  ACACAACCTACCTGTGGTGGGTAAATGGTCAGAGCCTCCCAGTCAGTCCCAGGCTGCAGC
  TGTCCAATGGCAACAGGACCCTCACTCTATTCAATGTCACAAGAAATGACGCAAGAGCCT
  ATGTATGTGGAATCCAGAACTCAGTGAGTGCAAACCGCAGTGACCCAGTCACCCTGGATG
  TCCTCTATGGGCCGGACACCCCCATCATTTCCCCCCCAGACTCGTCTTACCTTTCGGGAG
  CGAACCTCAACCTCTCCTGCCACTCGGCCTCTAACCCATCCCCGCAGTATTCTTGGCGTA
  TCAATGGGATACCGCAGCAACACACACAAGTTCTCTTTATCGCCAAAATCACGCCAAATA
  ATAACGGGACCTATGCCTGTTTTGTCTCTAACTTGGCTACTGGCCGCAATAATTCCATAG
  TCAAGAGCATCACAGTCTCTGCATCTGGAACTTCTCCTGGTCTCTCAGCTGGGGCCACTG
  TCGGCATCATGATTGGAGTGCTGGTTGGGGTTGCTCTGATATAGCAGCCCTGGTGTAGTT
  TCTTCATTTCAGGAAGACTGACAGTTGTTTTGCTTCTTCCTTAAAGCATTTGCAACAGCT
  ACAGTCTAAAATTGCTTCTTTACCAAGGATATTTACAGAAAAGACTCTGACCAGAGATCG
  AGACCATCCTAGCCAACATCGTGAAACCCCATCTCTACTAAAAATACAAAAATGAGCTGG
  GCTTGGTGGCGCGCACCTGTAGTCCCAGTTACTCGGGAGGCTGAGGCAGGAGAATCGCTT
  GAACCCGGGAGGTGGAGATTGCAGTGAGCCCAGATCGCACCACTGCACTCCAGTCTGGCA
  ACAGAGCAAGACTCCATCTCAAAAAGAAAAGAAAAGAAGACTCTGACCTGTACTCTTGAA
  TACAAGTTTCTGATACCACTGCACTGTCTGAGAATTTCCAAAACTTTAATGAACTAACTG
  ACAGCTTCATGAAACTGTCCCCCAAGATCAAGCAGAGAAAATAATTAATTTCATGGGACT
  AAATGAACTAATGAGGATAATATTTTCATAATTTTTTATTTGAAATTTTGCTGATTCTTT
  AAATGTCTTGTTTCCCAGATTTCAGGAAACTTTTTTTCTTTTAAGCTATCCACAGCTTAC
  AGCAATTTGATAAAATATACTTTTGTGAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004363 unedited
  GTTTTGATTTGTATACGACTCACTATAGGCGGACCGCGAATCANATCTGGTACCGGTCCG
  GAATTCCCGGGATGGACAGCAGACCAGACAGTCACAGCAGCCTTGACAAAACGTTCCTGG
  AACTCAAGCTCTTCTCCACAGAGGAGGACAGAGCAGACAGCAGAGACCATGGAGTCTCCC
  TCGGCCCCTCCCCACAGATGGTGCATCCCCTGGCAGAGGCTCCTGCTCACAGCCTCACTT
  CTAACCTTCTGGAACCCGCCCACCACTGCCAAGCTCACTATTGAATCCACGCCGTTCAAT
  GTCGCAGAGGGGAAGGAGGTGCTTCTACTTGTCCACAATCTGCCCCAGCATCTTTTTGGC
  TACAGCTGGTACAAAGGTGAAAGAGTGGATGGCAACCGTCAAATTATAGGATATGTAATA
  GGAACTCAACAAGCTACCCCAGGGCCCGCATACAGTGGTCGAGAGATAATATACCCCAAT
  GCATCCCTGCTGATCCAGAACATCATCCAGAATGACACAGGATTCTACACCCTACACGTC
  ATAAAGTCAGATCTTGTGAATGAAGAAGCAACTGGCCAGTTCCGGGTATACCCGGAGCTG
  CCCAAGCCCTCCATCTCCAGCAACAACTCCAAACCCGTGGAGGACAAGGATGCTGTGGCC
  TTCACCTGTGAACCTGAGACTCAGGACGCAACCTACCTGTGGTGGGTAAAACAATCAGAG
  CCTCCGGTCAGTCCCAGGCTGCAGCTGTCCAATGGCAACAGGACCCTCACTCTATNCAAT
  GTNCACAGAAATGACTCANNGCAGCTACAATGTGAAAACCCNAGACCCAGTGAGTGCCCA
  GCGCAGTGATTCAGTCATCCTGGGGTCCTCTATGGCCCNGGATGCCNCACCATTTCCCCT
  TCTAACACATCTTAAGATCAGGGGAAAATCTGAGCCTCT
  
• Restriction Sites: Please inquire
• ACCN: NM_004363
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004363.1, NP_004354.1
• RefSeq Size: 2974 bp
• RefSeq ORF: 2109 bp
• Locus ID: 1048
• Cytogenetics: 19q13.2
• Gene Summary: 'This gene encodes a cell surface glycoprotein that represents the founding member of the carcinoembryonic antigen (CEA) family of proteins. The encoded protein is used as a clinical biomarker for gastrointestinal cancers and may promote tumor development through its role as a cell adhesion molecule. Additionally, the encoded protein may regulate differentiation, apoptosis, and cell polarity. This gene is present in a CEA family gene cluster on chromosome 19. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Both variants 1 and 2 encode the same isoform. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.