PPM1D (BC042418) Human Untagged Clone

CAT#: SC126023

PPM1D (untagged)-Human protein phosphatase 1D magnesium-dependent, delta isoform, mRNA (cDNA clone MGC:35090 IMAGE:5167004), complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PPM1D
• Synonyms: p53-induced protein phosphatase 1; PP2C-DELTA; protein phosphatase 1D; protein phosphatase 1D magnesium-dependent, delta isoform; protein phosphatase 2C delta isoform; protein phosphatase Wip1; WIP1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for BC042418 edited
  CGCGCCCCCCCTTCTCCGGGTCCGCCCCCTCCCCCTTCTCGGCGTCGTCGAAGATAAACA
  ATAGTTGGCCGGCGAGCGCCTAGTGTGTCTCCCGCCGCCGGATTCGGCGGGCTGCGTGGG
  ACCGGCGGGATCCCGGCCAGCCGGCCATGGCGGGGCTGTACTCGCTGGGAGTGAGCGTCT
  TCTCCGACCAGGGCGGGAGGAAGTACATGGAGGACGTTACTCAAATCGTTGTGGAGCCCG
  AACCGACGGCTGAAGAAAAGCCCTCGCCGCGGCGGTCGCTGTCTCAGCCGTTGCCTCCGC
  GGCCGTCGCCGGCCGCCCTTCCCGGCGGCGAAGTCTCGGGGAAAGGCCCAGCGGTGGCAG
  CCCGAGAGGCTCGCGACCCTCTCCCGGACGCCGGGGCCTCGCCGGCACCTAGCCGCTGCT
  GCCGCCGCCGTTCCTCCGTGGCCTTTTTCGCCGTGTGCGACGGGCACGGCGGGCGGGAGG
  CGGCACAGTTTGCCCGGGAGCACTTGTGGGGTTTCATCAAGAAGCAGAAGGGTTTCACCT
  CGTCCGAGCCGGCTAAGGTTTGCGCTGCCATCCGCAAAGGCTTTCTCGCTTGTCACCTTG
  CCATGTGGAAGAAACTGGCGGAATGGCCAAAGACTATGACGGGTCTTCCTAGCACATCAG
  GGACAACTGCCAGTGTGGTCATCATTCGGGGCATGAAGATGTATGTAGCTCACGTAGGTG
  ACTCAGGGGTGGTTCTTGGAATTCAGGATGACCCGAAGGATGACTTTGTCAGAGCTGTGG
  AGGTGACACAGGACCATAAGCCAGAACTTCCCAAGGAAAGAGAACGAATCGAAGGACTTG
  GTGGGAGTGTAATGAACAAGTCTGGGGTGAATCGTGTAGTTTGGAAACGACCTCGACTCA
  CTCACAATGGACCTGTTAGAAGGAGCACAGTTATTGACCAGATTCCTTTTCTGGCAGTAG
  CAAGAGCACTTGGTGATTTGTGGAGCTATGATTTCTTCAGTGGTGAATTTGTGGTGTCAC
  CTGAACCAGACACAAGTGTCCACACTCTTGACCCTCAGAAGCACAAGTATATTATATTGG
  GGAGTGATGGACTTTGGAATATGATTCCACCACAAGATGCCATCTCAATGTGCCAGGACC
  AAGAGGAGAAAAAATACCTGATGGGTGAGCATGGACAATCTTGTGCCAAAATGCTTGTGA
  ATCGAGCATTGGGCCGCTGGAGGCAGCGTATGCTCCGAGCAGATAACACTAGTGCCATAG
  TAATCTGCATCTCTCCAGAAGTGGACAATCAGGGAAACTTTACCAATGAAGATGAGTTAT
  ACCTGAACCTGACTGACAGCCCTTCCTATAATAGTCAAGAAACCTGTGTGATGACTCCTT
  CCCCATGTTCTACACCACCAGTCAAGGATTTTGGATTTGAACTCGATTCAAGAAAGTGAT
  GTAACTTATTATCAGAGAGCCATCTTTACATCAATACTAATCTGAATGCCAGCCATGTGT
  ACAGCACTAATAAAAAGTCACTGGAGGAGGATCCATGGCCAAGGGTGAATTCTAAGGACC
  ATATACCTGCCCTGGTTCGTAGCAATGCCTTCTCAGAGAATTTTTTAGAGGTTTCAGCTG
  AGATAGCTCGAGAGAATGTCCAAGGTGTAGTCATACCCTCAAAAGATCCAGAACCACTTG
  AAGAAAATTGCGCTAAAGCCCTGACTTTAAGGATACATGATTCTTTGAATAATAGCCTTC
  CAATTGGCCTTGTGCCTACTAATTCAACAAACACTGTCATGGACCAAAAAAATTTGAAGA
  TGTCAACTCCTGGCCAAATGAAAGCCCAAGAAATTGAAAGAACCCCTCCAACAAACTTTA
  AAAGGACATTAGAAGAGTCCAATTCTGGCCCCCTGATGAAGAAGCATAGACGAAATGGCT
  TAAGTCGAAGTAGTGGTGCTCAGCCTGCAAGTCTCCCCACAACCTCACAGCGAAAGAACT
  CTGTTAAACTCACCATGCGACGCAGACTTAGGGGCCAGAAGAAAATTGGAAATCCTTTAC
  TTCATCAACACAGGAAAACTGTTTGTGTTTGCTGAAATGCATCTGGGAAATGAGGTTTTT
  CCAAACTTAGGATATAAGAGGGCTTTTTAAATTTGGTGCCGATGTTGAACTTTTTTTAAG
  GGGAGAAAATTAAAAGAAATATACAGTTTGACTTTTTGGAATTCAGCAGTTTTATCCTGG
  CCTTGTACTTGCTTGTATTGTAAATGTGGATTTTGTAGATGTTAGGGTATAAGTTGCTGT
  AAAATTTGTGTAAATTTGTATCCACACAAATTCAGTCTCTGAATACACAGTATTCAGAGT
  CTCTGATACACAGTAATTGTGACAATAGGGCTAAATGTTTAAAGAAATCAAAAGAATCTA
  TTAGATTTTAGAAAAACATTTAAACTTTTTAAAATACTTATTAAAAAATTTGTATAAGCC
  ACTTGTCTTGAAAACTGTGCAACTTTTTAAAGTAAATTATTAAGCAGACTGGAAAAGTGA
  TGTATTTTCATAGTGACCTGTGTTTCACTTAATGTTTCTTAGAGCCAAGTGTCTTTTAAA
  CATTATTTTTTATTTCTGATTTCATAATTCAGAACTAAATTTTTCATAGAAGTGTTGAGC
  CATGCTACAGTTAGTCTTGTCCCAATTAAAATACTATGCAGTATCTCTTACATCAGTAGC
  ATTTTTCTAAAACCTTAGTCATCAGATATGCTTACTAAATCTTCAGCATAGAAGGAAGTG
  TGTTTGCCTAAAACAATCTAAAACAATTCCCTTCTTTTTCATCCCAGACCAATGGCATTA
  TTAGGTCTTAAAGTAGTTACTCCCTTCTCGTGTTTGCTTAAAATATGTGAAGTTTTCCTT
  GCTATTTCAATAACAGATGGTGCTGCTAATTCCCAACATTTCTTAAATTATTTTATATCA
  TACAGTTTTCATTGATTATATGGGTATATATTCATCTAATAAATCAGTGAACTGTTAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC042418 unedited
  ATTTGTATACGACTTTTATAGGCGGCCGCGAATTCAGATCTGGTACCGGTCCGGAATTCC
  CGGGATCGCGCCCCCCCTTCTCCGGGTCCGCCCCCTCCCCCTTCTCGGCGTCGTCGAAGA
  TAAACAATAGTTGGCCGGCGAGCGCCTAGTGTGTCTCCCGCCGCCGGATTCGGCGGGCTG
  CGTGGGACCGGCGGGATCCCGGCCAGCCGGCCATGGCGGGGCTGTACTCGCTGGGAGTGA
  GCGTCTTCTCCGACCAGGGCGGGAGGAAGTACATGGAGGACGTTACTCAAATCGTTGTGG
  AGCCCGAACCGACGGCTGAAGAAAAGCCCTCGCCGCGGCGGTCGCTGTCTCAGCCGTTGC
  CTCCGCGGCCGTCGCCGGCCGCCCTTCCCGGCGGCGAAGTCTCGGGGAAAGGCCCAGCGG
  TGGCAGCCCGAGAGGCTCGCGACCCTCTCCCGGACGCCGGGGCCTCGCCGGCACCTAGCC
  GCTGCTGCCGCCGCCGTTCCTCCGTGGCCTTTTTCGCCGTGTGCGACGGGCACGGCGGGC
  GGGAGGCGGCACAGTTTGCCCGGGAGCACTTGTGGGGTTTCATCAAGAAGCAGAAGGGTT
  TCACCTCGTCCGAGCCGGGCTAAGGTTGCGCTGCCATCCCGCAAGGGCTTTCTCGCTTGT
  CACCTTGCCCTGGTGAAGAAAACTGGGCGATGGCCAAAGACTATGAACGGGTCTCCTTAC
  ACATCAGGGGACAACTGCCAGTGTGGTCATCATTCGGGCCTGAAAATGTATGTAACTCAC
  TTAGTGACTCAGGGTGGGTCTTGGAATCCAGATGAACCGAGGAGAATTTGTCAAAACTGG
  GGGGGGGCCCAGGACCTTAACCCAACCTCCCAGGAAGAAAACAAATCAAGGCTTGGTGGG
  GGTAATGAACAATTGGGGGAACCGGTATTTGGAACCACTCCCTCCT
  
• Restriction Sites: NotI-NotI
• ACCN: BC042418
• Insert Size: 3100
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: BC042418.1, AAH42418.1
• RefSeq Size: 3070
• Locus ID: 8493
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: p53 signaling pathway
• Gene Summary: The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. The expression of this gene is induced in a p53-dependent manner in response to various environmental stresses. While being induced by tumor suppressor protein TP53/p53, this phosphatase negatively regulates the activity of p38 MAP kinase, MAPK/p38, through which it reduces the phosphorylation of p53, and in turn suppresses p53-mediated transcription and apoptosis. This phosphatase thus mediates a feedback regulation of p38-p53 signaling that contributes to growth inhibition and the suppression of stress induced apoptosis. This gene is located in a chromosomal region known to be amplified in breast cancer. The amplification of this gene has been detected in both breast cancer cell line and primary breast tumors, which suggests a role of this gene in cancer development. [provided by RefSeq, Jul 2008]