Granzyme H (GZMH) (BC027974) Human Untagged Clone

CAT#: SC126062

GZMH (untagged)-Human granzyme H (cathepsin G-like 2, protein h-CCPX), mRNA (cDNA clone MGC:34849 IMAGE:5229314), complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GZMH
• Synonyms: CCP-X; CGL-2; CSP-C; CTLA1; CTSGL2; cytotoxic T-lymphocyte-associated serine esterase 1; cytotoxin serine protease-C; granzyme H; granzyme H (cathepsin G-like 2, protein h-CCPX)
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for BC027974 edited
  GGAGTCAACACCAACAGCTCTGACCTGGGCAGCCTTCCTGAGAAAATGCAGCCATTCCTC
  CTCCTGTTGGCCTTTCTTCTGACCCCTGGGGCTGGGACAGAGGAGATCATCGGGGGCCAT
  GAGGCCAAGCCCCACTCCCGCCCCTACATGGCCTTTGTTCAGTTTCTGCAAGAGAAGAGT
  CGGAAGAGGTGTGGCGGCATCCTAGTGAGAAAGGACTTTGTGCTGACAGCTGCTCACTGC
  CAGGGAAGCTCCATAAATGTCACCTTGGGGGCCCACAATATCAAGGAACAGGAGCGGACC
  CAGCAGTTTATCCCTGTGAAAAGACCCATCCCCCATCCAGCCTATAATCCTAAGAACTTC
  TCCAACGACATCATGCTACTGCAGCTGGAGAGAAAGGCCAAGTGGACCACAGCTGTGCGG
  CCTCTCAGGCTACCTAGCAGCAAGGCCCAGGTGAAGCCAGGGCAGCTGTGCAGTGTGGCT
  GGCTGGGGTTATGTCTCAATGAGCACTTTAGCAACCACACTGCAGGAAGTGTTGCTGACA
  GTGCAGAAGGACTGCCAGTGTGAACGTCTCTTCCATGGCAATTACAGCAGAGCCACTGAG
  ATTTGTGTGGGGGATCCAAAGAAGACACAGACCGGTTTCAAGGGGGACTCCGGGGGGCCC
  CTCGTGTGTAAGGACGTAGCCCAAGGTATTCTCTCCTATGGAAACAAAAAAGGGACACCT
  CCAGGAGTCTACATCAAGGTCTCACACTTCCTGCCCTGGATAAAGAGAACAATGAAGCGC
  CTCTAACAGCAGGCATGAGACTAACCTTCCTCTGGGCCTGACCATCTCTGGGACAGAGGC
  AAGAATCCCCAAGGGGTGGGCAGTCAGGGTTGCAGGACTGTAATAAATGGATCTCTGGTG
  TAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC027974 unedited
  NGGGTCGATTTTTGTATACGACTCATATAGGCGGCCGCGNAATTCNATCTGGTACCGGTC
  CGGAATTCCCGGGATGGAGTCAACACCAACAGCTCTGACCTGGGCAGCCTTCCTGAGAAA
  ATGCAGCCATTCCTCCTCCTGTTGGCCTTTCTTCTGACCCCTGGGGCTGGGACAGAGGAG
  ATCATCGGGGGCCATGAGGCCAAGCCCCACTCCCGCCCCTACATGGCCTTTGTTCAGTTT
  CTGCAAGAGAAGAGTCGGAAGAGGTGTGGCGGCATCCTAGTGAGAAAGGACTTTGTGCTG
  ACAGCTGCTCACTGCCAGGGAAGCTCCATAAATGTCACCTTGGGGGCCCACAATATCAAG
  GAACAGGAGCGGACCCAGCAGTTTATCCCTGTGAAAAGACCCATCCCCCATCCAGCCTAT
  AATCCTAAGAACTTCTCCAACGACATCATGCTACTGCAGCTGGAGAGAAAGGCCAAGTGG
  ACCACAGCTGTGCGGCCTCTCAGGCTACCTAGCAGCAAGGCCCAGGTGAAGCCAGGGCAG
  CTGTGCAGTGTGGCTGGCTGGGGTTATGTCTCAATGAGCACTTTAGCAACCACACTGCAG
  GAAGTGTTGCTGACAGTGCAGAAGGACTGCCAGTGTGAACGTCTCTTCCATGGCAATTAC
  AGCAGAGCCACTGAGATTTGTGTGGGGGATCCAAAGAAGACACAGACCGGTTTCAAGGGG
  GACTCCGGGGGGCCCCTCGTGTGTAAGGACGTAGCCCAAGGTATTCTCTCCTATGGAAAC
  AAAAAAGGGACACCTCCAGGAGTCTACATCAAGGTCTCACACTTCCTGCCCCTGGATAAG
  AGAACAATGAAGCGCCTCTAACAGCAGGCATGAGACTAACCTTCCTCTGGGCCTGACCAT
  CTCTGGA
  
• Restriction Sites: NotI-NotI
• ACCN: BC027974
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC027974.1, AAH27974.1
• RefSeq Size: 1047 bp
• Locus ID: 2999
• Cytogenetics: 14q12
• Protein Families: Druggable Genome, Protease
• Gene Summary: 'This gene encodes a member of the peptidase S1 family of serine proteases. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate a chymotrypsin-like protease. This protein is reported to be constitutively expressed in the NK (natural killer) cells of the immune system and may play a role in the cytotoxic arm of the innate immune response by inducing target cell death and by directly cleaving substrates in pathogen-infected cells. This gene is present in a gene cluster with another member of the granzyme subfamily on chromosome 14. [provided by RefSeq, Nov 2015]'