IDO1 (NM_002164) Human Untagged Clone

CAT#: SC126221

IDO1 (untagged)-Human indoleamine 2,3-dioxygenase 1 (IDO1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: IDO1
• Synonyms: IDO; IDO-1; INDO
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002164 edited
  AAACTCTTCAGACACTGAGGGGCACCAGAGGAGCAGACTACAAGAATGGCACACGCTATG
  GAAAACTCCTGGACAATCAGTAAAGAGTACCATATTGATGAAGAAGTGGGCTTTGCTCTG
  CCAAATCCACAGGAAAATCTACCTGATTTTTATAATGACTGGATGTTCATTGCTAAACAT
  CTGCCTGATCTCATAGAGTCTGGCCAGCTTCGAGAAAGAGTTGAGAAGTTAAACATGCTC
  AGCATTGATCATCTCACAGACCACAAGTCACAGCGCCTTGCACGTCTAGTTCTGGGATGC
  ATCACCATGGCATATGTGTGGGGCAAAGGTCATGGAGATGTCCGTAAGGTCTTGCCAAGA
  AATATTGCTGTTCCTTACTGCCAACTCTCCAAGAAACTGGAACTGCCTCCTATTTTGGTT
  TATGCAGACTGTGTCTTGGCAAACTGGAAGAAAAAGGATCCTAATAAGCCCCTGACTTAT
  GAGAACATGGACGTTTTGTTCTCATTTCGTGATGGAGACTGCAGTAAAGGATTCTTCCTG
  GTCTCTCTATTGGTGGAAATAGCAGCTGCTTCTGCAATCAAAGTAATTCCTACTGTATTC
  AAGGCAATGCAAATGCAAGAACGGGACACTTTGCTAAAGGCGCTGTTGGAAATAGCTTCT
  TGCTTGGAGAAAGCCCTTCAAGTGTTTCACCAAATCCACGATCATGTGAACCCAAAAGCA
  TTTTTCAGTGTTCTTCGCATATATTTGTCTGGCTGGAAAGGCAACCCCCAGCTATCAGAC
  GGTCTGGTGTATGAAGGGTTCTGGGAAGACCCAAAGGAGTTTGCAGGGGGCAGTGCAGGC
  CAAAGCAGCGTCTTTCAGTGCTTTGACGTCCTGCTGGGCATCCAGCAGACTGCTGGTGGA
  GGACATGCTGCTCAGTTCCTCCAGGACATGAGAAGATATATGCCACCAGCTCACAGGAAC
  TTCCTGTGCTCATTAGAGTCAAATCCCTCAGTCCGTGAGTTTGTCCTTTCAAAAGGTGAT
  GCTGGCCTGCGGGAAGCTTATGACGCCTGTGTGAAAGCTCTGGTCTCCCTGAGGAGCTAC
  CATCTGCAAATCGTGACTAAGTACATCCTGATTCCTGCAAGCCAGCAGCCAAAGGAGAAT
  AAGACCTCTGAAGACCCTTCAAAACTGGAAGCCAAAGGAACTGGAGGCACTGATTTAATG
  AATTTCCTGAAGACTGTAAGAAGTACAACTGAGAAATCCCTTTTGAAGGAAGGTTAATGT
  AACCCAACAAGAGCACATTTTATCATAGCAGAGACATCTGTATGCATTCCTGTCATTACC
  CATTGTAACAGAGCCACAAACTAATACTATGCAATGTTTTACCAATAATGCAATACAAAA
  GACCTCAAAATACCTGTGCATTTCTTGTAGGAAAACAACAAAAGGTAATTATGTGTAATT
  ATACTAGAAGTTTTGTAATCTGTATCTTATCATTGGAATAAAATGACATTCAATAAATAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002164 unedited
  ATGATTTGTATACCACTCTTATAGGCGGACCGCGATTCAGATCTGGTACCGGTCCGGAAT
  TCCCGGGATAAAACTCTTCAGACACTGAGGGGCACCAGAGGAGCAGACTACAAGAATGGC
  ACACGCTATGGAAAACTCCTGGACAATCAGTAAAGAGTACCATATTGATGAAGAAGTGGG
  CTTTGCTCTGCCAAATCCACAGGAAAATCTACCTGATTTTTATAATGACTGGATGTTCAT
  TGCTAAACATCTGCCTGATCTCATAGAGTCTGGCCAGCTTCGAGAAAGAGTTGAGAAGTT
  AAACATGCTCAGCATTGATCATCTCACAGACCACAAGTCACAGCGCCTTGCACGTCTAGT
  TCTGGGATGCATCACCATGGCATATGTGTGGGGCAAAGGTCATGGAGATGTCCGTAAGGT
  CTTGCCAAGAAATATTGCTGTTCCTTACTGCCAACTCTCCAAGAAACTGGAACTGCCTCC
  TATTTTGGTTTATGCAGACTGTGTCTTGGCAAACTGGAAGAAAAAGGATCCTAATAAGCC
  CCTGACTTATGAGAACATGGACGTTTTGTTCTCATTTCGTGATGGAGACTGCAGTAAAGG
  ATTCTTCCTGGTCTCTCTATTGGTGGAATAGCAGCTGCTTCTGCAATCAAAGTAATTCCT
  ACTGTATTCAAGCAATGCAAATGCAAGAACGGGACACTTTGCTAAAGGCGCTGNTGGAAA
  TAGCCTCTTGCTTGGAGAAAGCCCTTCAGTGTTTCACCAATCCACGATCATGTGACCCAA
  AGCTTTTTTCAGGGTCCTCGCTATATTTGGCTGGCTGGAAGGGAACCCCACTTTCAAACG
  TCTGGTGTATAAAGGGTTTTGGAAACCCAAGGAATTGCAGGGGAATGAAGGCCAACAACG
  TCTTCAAGGTTTGACGCCCGGTGGGCATCCCAGACGGT
  
• Restriction Sites: Please inquire
• ACCN: NM_002164
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002164.3, NP_002155.1
• RefSeq Size: 1655 bp
• RefSeq ORF: 1212 bp
• Locus ID: 3620
• Cytogenetics: 8p11.21
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Tryptophan metabolism
• Gene Summary: 'This gene encodes indoleamine 2,3-dioxygenase (IDO) - a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. This enzyme acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin. This enzyme is thought to play a role in a variety of pathophysiological processes such as antimicrobial and antitumor defense, neuropathology, immunoregulation, and antioxidant activity. Through its expression in dendritic cells, monocytes, and macrophages this enzyme modulates T-cell behavior by its peri-cellular catabolization of the essential amino acid tryptophan.[provided by RefSeq, Feb 2011]'