Neurofilament (NEFL) (BC066952) Human Untagged Clone

CAT#: SC126222

NEFL (untagged)-Human cDNA clone MGC:87388 IMAGE:5264373, complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NEFL
• Synonyms: CMT1F; CMT2E; FLJ53642; light molecular weight neurofilament protein; neurofilam; neurofilament, light polypeptide 68kDa; neurofilament-light; neurofilament protein, light chain; neurofilament subunit NF-L; neurofilament triplet L protein; NF-L; NF68; NFL
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for BC066952 edited
  GCACACAGCCATCCATCCTCCCCCTTCCCTCTCTCCCCTGTCCTCTCTCTCAGGGCTCCC
  ACCGCCGCCGCGGGCCGGGGAGCCACCGGCCGCCACCATGAGTTCCTTCAGCTACGAGCC
  GTACTACTCGACCTCCTACAAGCGGCGCTACGTGGAGACGCCCCGGGTGCACATCTCCAG
  CGTGCGCAGCGGCTACAGCACCGCACGCTCAGCTTACTCCAGCTACTCGGCGCCGGTGTC
  TTCCTCGCTGTCCGTGCGCCGCAGCTACTCCTCCAGCTCTGGATCGTTGATGCCCAGTCT
  GGAGAACCTCGACCTGAGCCAGGTAGCCGCCATCAGCAACGACCTCAAGTCCATCCGCAC
  GCAGGAGAAGGCGCAGCTCCAGGACCTCAATGACCGCTTCGCCAGCTTCATCGAGCGCGT
  GCACGAGCTGGAGCAGCAGAACAAGGTCCTGGAAGCCGAGCTGCTGGTGCTGCGCCAGAA
  GCACTCCGAGCCATCCCGCTTCCGGGCGCTGTACGAGCAGGAGATCCGCGACCTGCGCCT
  GGCGGCGGAAGATGCCACCAACGAGAAGCAGGCGCTCCAGGGCGAGCGCGAAGGGCTGGA
  GGAGACCCTGCGCAACCTGCAGGCGCGCTATGAAGAGGAGGTGCTGAGCCGCGAGGACGC
  CGAGGGCCGGCTGATGGAAGCGCGCAAAGGCGCCAAGAACACCGACGCCGTGCGCGCCGC
  CAAGGACGAGGTGTCCGAGAGCCGTCGTCTGCTCAAGGCCAAGACCCTGGAAATCGAAGC
  ATGCCGGGGCATGAATGAAGCGCTGGAGAAGCAGCTGCAGGAGCTGGAGGACAAGCAGAA
  CGCCGACATCAGCGTTATGCAGGACACGATCAACAAATTAGAAAATGAATTGAGGACCAC
  AAAGAGTGAAATGGCACGATACCTAAAAGAATACCAAGACCTCCTAACGTGAAGATGGCT
  TTGGATATTGAGATTGCAGCTTACAGGAAACTCTTGGAAGGCGAGGAGACCCGACTCAGT
  TTCACCAGCGTGGGAAGCATAACCAGTGGCTACTCCCAGAGCTCCCAGGTCTTTGGCCGA
  TCTGCCTACGGCGGTTTACAGACCAGCTCCTATCTGATGTCCACCCGCTCCTTCCCGTCC
  TACTACACCAGCCATGTCCAAGAGGAGCAGATCGAAGTGGAGGAAACCATTGAGGCTGCC
  AAGGCTGAGGAAGCCAAGGATGAGCCCCCCTCTGAAGGAGAAGCCGAGGAGGAGGAGAAG
  GACAAGGAAGAGGCCGAGGAAGAGGAGGCAGCTGAAGAGGAAGAAGCTGCCAAGGAAGAG
  TCTGAAGAAGCAAAAGAAGAAGAAGAAGGAGGTGAAGGTGAAGAAGGAGAGGAAACCAAA
  GAAGCTGAAGAGGAGGAGAAGAAAGTTGAAGGTGCTGGGGAGGAACAAGCAGCTAAGAAG
  AAAGATTGAACCCCCATTTCCTTAATTATTTCAGGAATAATTCTCCCGAAATCAGGTCAA
  CCCCATCACCAACCAACCAACCAGTTGAGTTCCAGATTCTATGTGAATTAAAAAGTCAAT
  ATATGTATAATTCTGAGATGACTTAGGTTGGACATTCAATGTTGTGCTATGAATTTCCTC
  TTTATGCAGAGTATCTGTTTGCTTGCAGAGTGGCTTTCTGGCTTGCTGCCAGCCTGTGCA
  TGGTCCACGCTTATGAGTTCAGGATCTACGGCAATGTGAATCATTCAGATGTTTACAATA
  AAAAACACCACATGAGTAAATGAATTCACTAATGTTAATGTTAAACTTCATGGAAAAATA
  GTCCTTTGAACCTTCGGTGGTTAGCAATTAAAGACCCTGAGTTATGTGCAATAAATAGTA
  AATAAAGTTATACCGAACGAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC066952 unedited
  GAATTGGGGGTCGAATTTGTAAACGACTCATATAGGCGGCCGCATAACTTCGTATAGCAT
  ACATTATACGAAGTTATGGATCAGGCCAAATCGGCCGAGCTCGAATTCGTCGAGAGCGGG
  CACACAGCCATCCATCCTCCCCCTTCCCTCTCTCCCCTGTCCTCTCTCTCAGGGCTCCCA
  CCGCCGCCGCGGGCCGGGGAGCCACCGGCCGCCACCATGAGTTCCTTCAGCTACGAGCCG
  TACTACTCGACCTCCTACAAGCGGCGCTACGTGGAGACGCCCCGGGTGCACATCTCCAGC
  GTGCGCAGCGGCTACAGCACCGCACGCTCAGCTTACTCCAGCTACTCGGCGCCGGTGTCT
  TCCTCGCTGTCCGTGCGCCGCAGCTACTCCTCCAGCTCTGGATCGTTGATGCCCAGTCTG
  GAGAACCTCGACCTGAGCCAGGTAGCCGCCATCAGCAACGACCTCAAGTCCATCCGCACG
  CAGGAGAAGGCGCAGCTCCAGGACCTCAATGACCGCTTCGCCAGCTTCATCGAGCGCGTG
  CACGAGCTGGAGCAGCAGAACAAGGTCCTGGAAGCCGAGCTGCTGGTGCTGCGCCAGAAG
  CACTCCGAGCCATCCCGCTTCCGGGCGCTGTACGAGCAGGAGATCCGCGACCTGCGCCTG
  GCGGCGGAAGATGCCACCAACGAGAAGCANGCGCTCCAGGGCGAGCGCGAAGGGCTGGAG
  GAGACCCTGCGCAACCTGCNAGCGCGCTATGAAGAGGAGGTGCTGAGCCGCGAGGACGCC
  CGAGGCCGGCTGATGGAAGCGCGCAAGGCGCCAAAACACCGACGCCGTGCGCGCCGCCNA
  GGACGAGGTGTCCC
  
• Restriction Sites: NotI-NotI
• ACCN: BC066952
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC066952.1, AAH66952.1
• RefSeq Size: 1895 bp
• Locus ID: 4747
• Cytogenetics: 8p21.2
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS
• Protein Pathways: Amyotrophic lateral sclerosis (ALS)
• Gene Summary: 'Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq, Oct 2008]'