PEPD (BC028295) Human Untagged Clone
CAT#: SC126231
PEPD (untagged)-Human peptidase D, mRNA (cDNA clone MGC:24893 IMAGE:4470066), complete cds
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Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Symbol | PEPD |
Synonyms | imidodipeptidase; MGC10905; peptidase D; PROLIDASE; proline dipeptidase |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene sequence for BC028295 edited
CGAACATGGCGGCGGCCACCGGACCCTCGTTTTGGCTGGGGAATGAAACCCTGAAGGTGC CGCTGGCGCTCTTTGCCTTGAACCGGCAGCGCCTGTGTGAGCGGCTGCGGAAGAACCCTG CTGTGCAGGCCGGCTCCATCGTGGTCCTGCAGGGCGGGGAGGAGACTCAGCGCTACTGCA CCGACACCGGGGTCCTCTTCCGCCAGGAGTCCTTCTTTCACTGGGCGTTCGGTGTCACTG AGCCAGGCTGCTATGGTGTCATCGATGTTGACACTGGGAAGTCGACCCTGTTTGTGCCCA GGCTTCCTGCCAGCCATGCCACCTGGATGGGAAAGATCCATTCCAAGGAGCACTTCAAGG AGAAGTATGCCGTGGACGACGTCCAGTACGTAGATGAGATTGCCAGCGTCCTGACGTCAC AGAAGCCCTCTGTCCTCCTCACTTTGCGTGGCGTCAACACGGACAGCGGCAGTGTCTGCA GGGAGGCCTCCTTTGACGGCATCAGCAAGTTCGAAGTCAACAATACCATTCTTCACCCAG AGATCGTTGAGTGCCGAGTGTTTAAGACGGATATGGAGCTGGAGGTTCTGCGCTATACCA ATAAAATCTCCAGCGAGGCCCACCGTGAGGTAATGAAGGCTGTAAAAGTGGGAATGAAAG AATATGAGTTGGAAAGCCTCTTCGAGCACTACTGCTACTCCCGGGGCGGCATGCGCCACA GCTCCTACACCTGCATCTGCGGCAGTGGTGAGAACTCAGCCGTGCTACACTACGGACACG CCGGAGCTCCCAACGACCGAACGATCCAGAATGGGGATATGTGCCTGTTCGACATGGGCG GTGAGTATTACTGCTTCGCTTCCGACATCACCTGCTCCTTTCCCGCCAACGGCAAGTTCA CTGCAGACCAGAAGGCCGTCTATGAGGCAGTGCTGCGGAGCTCCCGTGCCGTCATGGGTG CCATGAAGCCAGGTGTCTGGTGGCCTGACATGCACCGCCTGGCTGACCGCATCCACCTGG AGGAGCTGGCCCACATGGGCATCCTGAGCGGCAGCGTGGACGCCATGGTCCAGGCTCACC TGGGGGCCGTGTTTATGCCTCACGGGCTTGGCCACTTCCTGGGCATTGACGTGCACGACG TGGGAGGCTACCCAGAGGGCGTGGAGCGCATCGACGAGCCCGGCCTGCGGAGCCTGCGCA CTGCACGGCACCTGCAGCCAGGCATGGTGCTCACCGTGGAGCCGGGCATCTACTTCATCG ACCACCTCCTGGATGAGGCCCTGGCGGACCCGGCCCGCGCCTCCTTCTTTAACCGCGAGG TCCTGCAGCGCTTTCGCGGTTTTGGCGGGGTCCGCATCGAGGAGGACGTCGTGGTGACTG ACAGCGGCATAGAGCTGCTGACCTGCGTGCCCCGCACTGTGGAAGAGATTGAAGCATGCA TGGCAGGCTGTGACAAGGCCTTTACCCCCTTCTCTGGCCCCAAGTAGAGCCAGCCAGAAA TCCCAGCGCACCTGGGGGCCTGGCCTTGCAACCTCTTTCCGTGATGGGCAGCCTGCTGGT CAGCACTCCAGTAGCGAGAGACGGCACCCAGAATCAGATCCCAGCTTCGGCATTTGATCA GACCAAACAGTGCTGTTTCCCGGGGAGGAAACACTTTTTTAATTACCCTTTTGCAGGCTC CCACCTTTAATCTGTTTCATACCTTGCTTATTAAATGAGCGACTTAAAATGATTGAAAAT AATGCTGTTCTTTAGTAGCAACTAAAATGTGTCTTGCTGTCATTTATATTCCTTTTCCCA GGAAAGAAGCATTTCTGATACTTTACTTTCTGTCAAAAATCAATATGCAGAATGGCATTT GCAATAAAAGGTTTCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA >OriGene 5' read for BC028295 unedited
GAGTTTGAATTTGTATACGACTTATATAGGCGGCCGCGNAATTCNATCTGGTACCGGTCC GGAATTCCCGGGATATCGTCGACCCACGCGTCCGCGAACATGGCGGCGGCCACCGGACCC TCGTTTTGGCTGGGGAATGAAACCCTGAAGGTGCCGCTGGCGCTCTTTGCCTTGAACCGG CAGCGCCTGTGTGAGCGGCTGCGGAAGAACCCTGCTGTGCAGGCCGGCTCCATCGTGGTC CTGCAGGGCGGGGAGGAGACTCAGCGCTACTGCACCGACACCGGGGTCCTCTTCCGCCAG GAGTCCTTCTTTCACTGGGCGTTCGGTGTCACTGAGCCAGGCTGCTATGGTGTCATCGAT GTTGACACTGGGAAGTCGACCCTGTTTGTGCCCAGGCTTCCTGCCAGCCATGCCACCTGG ATGGGAAAGATCCATTCCAAGGAGCACTTCAAGGAGAAGTATGCCGTGGACGACGTCCAG TACGTAGATGAGATTGCCAGCGTCCTGACGTCACAGAAGCCCTCTGTCCTCCTCACTTTG CGTGGCGTCAACACGGACAGCGGCAGTGTCTGCAGGGAGGCCTCCTTTGACGGCATCAGC AAGTTCGAAGTCAACAATACCATTCTTCACCCAGAGATCGTTGAGTGCCGAGTGTTTAAG ACGGATATGGAGCTGGAGGTTCTGCGCTATACCAATAAAATCTCCAGCGAGGCCCACCGT GAGGTAATGAAGGCTGTANAAGTGGGAATGAAAGATATGAGTTGGNAAGCCTCTTCGAGC ACTACTGCTACTCCCGGGCGGCATGCGCCACAGCTCCTACACCTGCATCTGCGGCAGTGG TGAGGACTCAGCCGTGCTACACTACCGACACGCCGGGACTNCCNACGACCA |
Restriction Sites | NotI-NotI |
ACCN | BC028295 |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | BC028295.1, AAH28295.1 |
RefSeq Size | 1908 bp |
Locus ID | 5184 |
Cytogenetics | 19q13.11 |
Protein Families | Druggable Genome, Protease |
Gene Summary | 'This gene encodes a member of the peptidase family. The protein forms a homodimer that hydrolyzes dipeptides or tripeptides with C-terminal proline or hydroxyproline residues. The enzyme serves an important role in the recycling of proline, and may be rate limiting for the production of collagen. Mutations in this gene result in prolidase deficiency, which is characterized by the excretion of large amount of di- and tri-peptides containing proline. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009]' |
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