PEPD (BC028295) Human Untagged Clone

CAT#: SC126231

PEPD (untagged)-Human peptidase D, mRNA (cDNA clone MGC:24893 IMAGE:4470066), complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PEPD
• Synonyms: imidodipeptidase; MGC10905; peptidase D; PROLIDASE; proline dipeptidase
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for BC028295 edited
  CGAACATGGCGGCGGCCACCGGACCCTCGTTTTGGCTGGGGAATGAAACCCTGAAGGTGC
  CGCTGGCGCTCTTTGCCTTGAACCGGCAGCGCCTGTGTGAGCGGCTGCGGAAGAACCCTG
  CTGTGCAGGCCGGCTCCATCGTGGTCCTGCAGGGCGGGGAGGAGACTCAGCGCTACTGCA
  CCGACACCGGGGTCCTCTTCCGCCAGGAGTCCTTCTTTCACTGGGCGTTCGGTGTCACTG
  AGCCAGGCTGCTATGGTGTCATCGATGTTGACACTGGGAAGTCGACCCTGTTTGTGCCCA
  GGCTTCCTGCCAGCCATGCCACCTGGATGGGAAAGATCCATTCCAAGGAGCACTTCAAGG
  AGAAGTATGCCGTGGACGACGTCCAGTACGTAGATGAGATTGCCAGCGTCCTGACGTCAC
  AGAAGCCCTCTGTCCTCCTCACTTTGCGTGGCGTCAACACGGACAGCGGCAGTGTCTGCA
  GGGAGGCCTCCTTTGACGGCATCAGCAAGTTCGAAGTCAACAATACCATTCTTCACCCAG
  AGATCGTTGAGTGCCGAGTGTTTAAGACGGATATGGAGCTGGAGGTTCTGCGCTATACCA
  ATAAAATCTCCAGCGAGGCCCACCGTGAGGTAATGAAGGCTGTAAAAGTGGGAATGAAAG
  AATATGAGTTGGAAAGCCTCTTCGAGCACTACTGCTACTCCCGGGGCGGCATGCGCCACA
  GCTCCTACACCTGCATCTGCGGCAGTGGTGAGAACTCAGCCGTGCTACACTACGGACACG
  CCGGAGCTCCCAACGACCGAACGATCCAGAATGGGGATATGTGCCTGTTCGACATGGGCG
  GTGAGTATTACTGCTTCGCTTCCGACATCACCTGCTCCTTTCCCGCCAACGGCAAGTTCA
  CTGCAGACCAGAAGGCCGTCTATGAGGCAGTGCTGCGGAGCTCCCGTGCCGTCATGGGTG
  CCATGAAGCCAGGTGTCTGGTGGCCTGACATGCACCGCCTGGCTGACCGCATCCACCTGG
  AGGAGCTGGCCCACATGGGCATCCTGAGCGGCAGCGTGGACGCCATGGTCCAGGCTCACC
  TGGGGGCCGTGTTTATGCCTCACGGGCTTGGCCACTTCCTGGGCATTGACGTGCACGACG
  TGGGAGGCTACCCAGAGGGCGTGGAGCGCATCGACGAGCCCGGCCTGCGGAGCCTGCGCA
  CTGCACGGCACCTGCAGCCAGGCATGGTGCTCACCGTGGAGCCGGGCATCTACTTCATCG
  ACCACCTCCTGGATGAGGCCCTGGCGGACCCGGCCCGCGCCTCCTTCTTTAACCGCGAGG
  TCCTGCAGCGCTTTCGCGGTTTTGGCGGGGTCCGCATCGAGGAGGACGTCGTGGTGACTG
  ACAGCGGCATAGAGCTGCTGACCTGCGTGCCCCGCACTGTGGAAGAGATTGAAGCATGCA
  TGGCAGGCTGTGACAAGGCCTTTACCCCCTTCTCTGGCCCCAAGTAGAGCCAGCCAGAAA
  TCCCAGCGCACCTGGGGGCCTGGCCTTGCAACCTCTTTCCGTGATGGGCAGCCTGCTGGT
  CAGCACTCCAGTAGCGAGAGACGGCACCCAGAATCAGATCCCAGCTTCGGCATTTGATCA
  GACCAAACAGTGCTGTTTCCCGGGGAGGAAACACTTTTTTAATTACCCTTTTGCAGGCTC
  CCACCTTTAATCTGTTTCATACCTTGCTTATTAAATGAGCGACTTAAAATGATTGAAAAT
  AATGCTGTTCTTTAGTAGCAACTAAAATGTGTCTTGCTGTCATTTATATTCCTTTTCCCA
  GGAAAGAAGCATTTCTGATACTTTACTTTCTGTCAAAAATCAATATGCAGAATGGCATTT
  GCAATAAAAGGTTTCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC028295 unedited
  GAGTTTGAATTTGTATACGACTTATATAGGCGGCCGCGNAATTCNATCTGGTACCGGTCC
  GGAATTCCCGGGATATCGTCGACCCACGCGTCCGCGAACATGGCGGCGGCCACCGGACCC
  TCGTTTTGGCTGGGGAATGAAACCCTGAAGGTGCCGCTGGCGCTCTTTGCCTTGAACCGG
  CAGCGCCTGTGTGAGCGGCTGCGGAAGAACCCTGCTGTGCAGGCCGGCTCCATCGTGGTC
  CTGCAGGGCGGGGAGGAGACTCAGCGCTACTGCACCGACACCGGGGTCCTCTTCCGCCAG
  GAGTCCTTCTTTCACTGGGCGTTCGGTGTCACTGAGCCAGGCTGCTATGGTGTCATCGAT
  GTTGACACTGGGAAGTCGACCCTGTTTGTGCCCAGGCTTCCTGCCAGCCATGCCACCTGG
  ATGGGAAAGATCCATTCCAAGGAGCACTTCAAGGAGAAGTATGCCGTGGACGACGTCCAG
  TACGTAGATGAGATTGCCAGCGTCCTGACGTCACAGAAGCCCTCTGTCCTCCTCACTTTG
  CGTGGCGTCAACACGGACAGCGGCAGTGTCTGCAGGGAGGCCTCCTTTGACGGCATCAGC
  AAGTTCGAAGTCAACAATACCATTCTTCACCCAGAGATCGTTGAGTGCCGAGTGTTTAAG
  ACGGATATGGAGCTGGAGGTTCTGCGCTATACCAATAAAATCTCCAGCGAGGCCCACCGT
  GAGGTAATGAAGGCTGTANAAGTGGGAATGAAAGATATGAGTTGGNAAGCCTCTTCGAGC
  ACTACTGCTACTCCCGGGCGGCATGCGCCACAGCTCCTACACCTGCATCTGCGGCAGTGG
  TGAGGACTCAGCCGTGCTACACTACCGACACGCCGGGACTNCCNACGACCA
  
• Restriction Sites: NotI-NotI
• ACCN: BC028295
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC028295.1, AAH28295.1
• RefSeq Size: 1908 bp
• Locus ID: 5184
• Cytogenetics: 19q13.11
• Protein Families: Druggable Genome, Protease
• Gene Summary: 'This gene encodes a member of the peptidase family. The protein forms a homodimer that hydrolyzes dipeptides or tripeptides with C-terminal proline or hydroxyproline residues. The enzyme serves an important role in the recycling of proline, and may be rate limiting for the production of collagen. Mutations in this gene result in prolidase deficiency, which is characterized by the excretion of large amount of di- and tri-peptides containing proline. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009]'