HLAC (HLA-C) (BC008457) Human Untagged Clone

CAT#: SC126265

HLA (untagged)-Human major histocompatibility complex, class I, C, mRNA (cDNA clone MGC:14707 IMAGE:4249847), complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HLA
• Synonyms: HLA-JY3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for BC008457 edited
  AGACGCCGAGATGCGGGTCATGGCGCCCCGAACCCTCATCCTGCTGCTCTCGGGAGCCCT
  GGCCCTGACCGAGACCTGGGCCGGCTCCCACTCCATGAGGTATTTCTACACCGCTGTGTC
  CCGGCCCGGCCGCGGGGAGCCCCACTTCATCGCAGTGGGCTACGTGGACGACACGCAGTT
  CGTGCGGTTCGACAGCGACGCCGCGAGTCCGAGAGGGGAGCCGCGGGCGCCGTGGGTGGA
  GCAGGAGGGGCCGGAGTATTGGGACCGGGAGACACAGAAGTACAAGCGCCAGGCACAGAC
  TGACCGAGTGAGCCTGCGGAACCTGCGCGGCTACTACAACCAGAGCGAGGCCAGGTCTCA
  CATCATCCAGAGGATGTATGGCTGCGACGTGGGGCCCGACGGGCGCCTCCTCCGCGGGTA
  TGACCAGTACGCCTACGACGGCAAGGATTACATCGCCCTGAACGAGGATCTGCGCTCCTG
  GACCGCCGCGGACACGGCGGCTCAGATCACCCAGCGCAAGTGGGAGGCGGCCCGTGAGGC
  GGAGCAGCTGAGAGCCTACCTGGAGGGCCTGTGCGTGGAGTGGCTCCGCAGATACCTGAA
  GAATGGGAAGGAGACGCTGCAGCGCGCGGAACACCCAAAGACACACGTGACCCACCATCC
  CGTCTCTGACCATGAGGCCACCCTGAGGTGCTGGGCCCTGGGCTTCTACCCTGCGGAGAT
  CACACTGACCTGGCAGTGGGATGGGGAGGACCAAACTCAGGACACTGAGCTTGTGGAGAC
  CAGGCCAGCAGGAGATGGAACCTTCCAGAAGTGGGCAGCTGTGGTGGTGCCTTCTGGAGA
  AGAGCAGAGATACACGTGCCATGTGCAGCACGAGGGGCTGCCGGAGCCCCTCACCCTGAG
  ATGGGAGCCGTCTTCCCAGCCCACCATCCCCATCGTGGGCATCGTTGCTGGCCTGGCTGT
  CCTGGCTGTCCTAGCTGTCCTAGGAGCTGTGGTGGCTGTTGTGATGTGTAGGAGGAAGAG
  CTCAGGGCATTTTCTTCCCACAGGTGGAAAAGGAGGGAGCTGCTCTCAGGCTGCGTCCAG
  CAACAGTGCCCAGGGCTCTGATGAGTCTCTCATCGCTTGTAAAGCCTGAGACAGCTGCCT
  GTGTGGGACTGAGATGCAGGATTTCTTCACACCTCTCCTTTGTGACTTCAAGAGCCTCTG
  GCATCTCTTTCTGCAAAGGCATCTGAATGTGTCTGCGTTCCTGTTAGCATAATGTGAGGA
  GGTGGACAGACAGCCCACCCCCGTGTCCACCGTGACCCCTGTCCCCACACTGACCTGTGT
  TCCCTCCCCGATCATCTTTCCTGTTCCAGAGAAGTGGGCTGGATGTCTCCATCTCTGTCT
  CAACTTCATGGTGCGCTGAGCTGCAACTTCTTACTTCCCTAATGAAGTTAGGAACCTGAA
  TATAAATTTGTTTTCTCAAATATTTGCTATGAAGGGTTGATGGATTAATTAAATAAGTCA
  ATTCCTGGAAGTTGAGAGAGCAAATAAAGACCTGAGAACTTTCCAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC008457 unedited
  TCACGAATTTGTAATACGACTCACTATAGGCGGCGCGCGAATTCGCCATTACGGCCGGGG
  AGACGCCCAGATGCGGGTCATGGGGCCCCGAACCCTCATCCTGCTGCTCTCGGGAGCCCT
  GGCCCTGACCGAGACCTGGGCCGGCTCCCACTCCATGAGGTATTTCTACACCGCTGTGTC
  CCGGCCCGGCCGCGGGGAGCCCCACTTCATCGCAGTGGGCTACGTGGACGACACGCAGTT
  CGTGCGGTTCGACAGCGACGCCGCGAGTCCGAGAGGGGAGCCGCGGGCGCCGTGGGTGGA
  GCANGAGGGGCCGGAGTATTGGGACCGGGAGACACAGAAGTACAAGCGCCAGGCACAGAC
  TGACCGAGTGAGCCTGCGGAACCTGCGCGGCTACTACAACCAGAGCGAGGCCAGGTCTCA
  CATCATCCAAAGGATGTATGGCTGCGACGTGGGGCCCGACGGGCGCCTCCTCCGCGGGTA
  TGACCAGTACGCCTACCACGGCCAGGATTACATCGCCCTGAACGAGGATCTGCGCTCCTG
  GACCGGCGCGGACACGGCGGCTCAGATCACCCAGCGCAAGTGGGAGGCGGCCCCTGAGGC
  GGAGCANCTGAGAGCCTACCTGGAGGGCCTGTGCGTGGAGTGGCTCCCCANATACCTGAA
  GAATGGGAAGGGGACCCTGCANCGCGCGGAGACACCCAAGGACACCCGTGACCCACCATC
  CCGTCTTTGGACCATGAGGCCCCCCTGAGGGGGCTGGGCCTGGGGTTCTACCCGGGGGAG
  ATCACCCTTAACTGGGAGAGGGGGTGGGGAGGACCAAACTCAGGGACGTGGCTTTTGGGG
  AACCCCGCCCCCCGGGGGAGTGGGACCTTTCCAAAGTGGGGGACGTGGGGGGGTGCCCTT
  CTGGGGAGGAGCAAAATAACCCTGGCCATGGTGGAGACAGGGGGGCGCCGCGGACCCCTT
  CACCTGGAAGAGGGGCCGCCT
  
• Restriction Sites: Please inquire
• ACCN: BC008457
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC008457.1, AAH08457.1
• RefSeq Size: 1574 bp
• Locus ID: 3107
• Cytogenetics: 6p21.33
• Protein Families: Secreted Protein, Transmembrane
• Protein Pathways: Allograft rejection, Antigen processing and presentation, Autoimmune thyroid disease, Cell adhesion molecules (CAMs), Endocytosis, Graft-versus-host disease, Natural killer cell mediated cytotoxicity, Type I diabetes mellitus, Viral myocarditis
• Gene Summary: 'HLA-C belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. About 6000 HLA-C alleles have been described. The HLA system plays an important role in the occurrence and outcome of infectious diseases, including those caused by the malaria parasite, the human immunodeficiency virus (HIV), and the severe acute respiratory syndrome coronavirus (SARS-CoV). The structural spike and the nucleocapsid proteins of the novel coronavirus SARS-CoV-2, which causes coronavirus disease 2019 (COVID-19), are reported to contain multiple Class I epitopes with predicted HLA restrictions. Individual HLA genetic variation may help explain different immune responses to a virus across a population.[provided by RefSeq, Aug 2020]'