PYCR1 (BC022244) Human Untagged Clone

CAT#: SC126267

PYCR1 (untagged)-Human pyrroline-5-carboxylate reductase 1 (cDNA clone MGC:22061 IMAGE:4420238), complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PYCR1
• Synonyms: P5C; P5CR; P5C reductase; PIG45; PP222; proliferation-inducing protein 45; PYCR; pyrroline-5-carboxylate reductase 1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for BC022244 edited
  GCCCTGGCCAAGGGCTTCACAGCAGCAGGCGTCTTGGCTGCCCACAAGATAATGGCTAGC
  TCCCCAGACATGGACCTGGCCACAGTTTCTGCTCTCAGGAAGATGGGGGTGAAGTTGACA
  CCCCACAACAAGGAGACGGTGCAGCACAGTGATGTGCTCTTCCTGGCTGTGAAGCCACAC
  ATCATCCCCTTCATCCTGGATGAAATAGGCGCCGACATTGAGGACAGACACATTGTGGTG
  TCCTGCGCGGCCGGCGTCACCATCAGCTCCATTGAGAAGAAGCTGTCAGCGTTTCGGCCA
  GCCCCCAGGGTCATCCGCTGCATGACCAACACTCCAGTCGTGGTGCGGGAGGGGGCCACC
  GTGTATGCCACAGGCACGCACGCCCAGGTGGAGGACGGGAGGCTCATGGAGCAGCTGCTG
  AGCAGCGTGGGCTTCTGCACGGAGGTGGAAGAGGACCTGATTGATGCCGTCACGGGGCTC
  AGTGGCAGCGGCCCCGCCTACGCATTCACAGCCCTGGATGCCCTGGCTGATGGGGGTGTG
  AAGATGGGACTTCCAAGGCGCCTGGCAGTCCGCCTCGGGGCCCAGGCCCTCCTGGGGGCT
  GCCAAGATGCTGCTGCACTCAGAACAGCACCCAGGCCAGCTCAAGGACAACGTCAGCTCT
  CCTGGTGGGGCCACCATCCATGCCTTGCATGTGCTGGAGAGTGGGGGCTTCCGCTCCCTG
  CTCATCAACGCTGTGGAGGCCTCCTGCATCCGCACACGGGAGCTGCAGTCCATGGCTGAC
  CAGGAGCAGGTGTCACCAGCCGCCATCAAGAAGACCATCCTGGACAAGGTGAAGCTGGAC
  TCCCCTGCAGGGACCGCTCTGTCGCCTTCTGGCCACACCAAGCTGCTCCCCCGCAGCCTG
  GCCCCAGCGGGCAAGGATTGACACGTCCTGCCTGACCACCATCCTGCCACCACCTTCTCT
  TCTCTTGTCACTAGGGGGACTAGGGGGTCCCCAAAGTGGCCCACTTTCTGTGGCTCTGAT
  CAGCGCAGGGGCCAGCCAGGGACATAGCCAGGGAGGGGCCACATCACTTCCCACTGGAAA
  TCTCTGTGGTCTGCAAGTGCTTCCCAGCCCAGAACAGGGGTGGATTCCCCAACCTCAACC
  TCCTTTCTTCTCTGCTCCCAAACCATGTCAGGACCACCTTCCTCTAGAGCTCGGGAGCCC
  GGAGGGTCTTCACCCACTCCTACTCCAGTATCAGCTGGCACGGGCTCCTTCCTGAGAGCA
  AAGGTCAAGGACCCCCTCTGTGAAGGCTCAGCAGAGGTGGGATCCCACGCCCCCTCCCGG
  CCCCTCCCTGCCCTCCATTCAGGGAGAAACCTCTCCTTCCCGTGTGAGAAGGGCCAGAGG
  GTCCAGGCATCCCAAGTCCAGCGTGAAGGGCCACAGCCCCTCTTGGCTGCCAAGCACGCA
  GATCCCATGGACATTTGGGGAAAGGGCTCCTTGGGCTGCTGGTGAACTTCTGTGGCCACC
  ACCTCCTGCTCCTGACCTCCCTGGGAGGGTGCTATCAGTTCTGTCCTGGCCCTTTCAGTT
  TTATAAGTTGGTTTCCAGCCCCCAGTGTCCTGACTTCTGTCTGCCACATGAGGAGGGAGG
  CCCTGCCTGTGTGGGAGGGTGGTTACTGTGGGTGGAATAGTGGAGGCCTTCAACTGATTA
  GACAAGGCCCGCCCACATCTTGGAGGGCATCTGCCTTACTGATTAAAATGTCAATGTAAT
  CTAAAAAAAAAAAAAAA
  
• Restriction Sites: Please inquire
• ACCN: BC022244
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC022244.1, AAH22244.1
• RefSeq Size: 1757 bp
• Locus ID: 5831
• Cytogenetics: 17q25.3
• Protein Pathways: Arginine and proline metabolism, Metabolic pathways
• Gene Summary: 'This gene encodes an enzyme that catalyzes the NAD(P)H-dependent conversion of pyrroline-5-carboxylate to proline. This enzyme may also play a physiologic role in the generation of NADP(+) in some cell types. The protein forms a homopolymer and localizes to the mitochondrion. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]'