TLE3 (BC041831) Human Untagged Clone

CAT#: SC126383

TLE3 (untagged)-Human transducin-like enhancer of split 3 (E(sp1) homolog, Drosophila), mRNA (cDNA clone MGC:43497 IMAGE:5268249), complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TLE3
• Synonyms: enhancer of split groucho 3; ESG; ESG3; FLJ39460; GRG3; HsT18976; KIAA1547; transducin-like enhancer of split 3 (E(sp1) homolog, Drosophila); transducin-like enhancer of split 3, homolog of Drosophila E(sp1); transducin-like enhancer protein 3
• Vector: PCMV6-Neo
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for BC041831 edited
  AATTCCGCGCGAGTGGCGCGGGGGGCTGCTTGTGTTCCAGGGGGAGCTGTTCACGCGGTC
  CCCGGCCCCGCTCTGAAGTCTCCCAACTCACCCGGCTTGTCTCCCGCCCCCGCCTTACTG
  GGGAGAGGGAGGCCCTTGGTGAAAGGCATGGGAGCCCCGCTGCCGACGGGGAGAGATGGG
  GCGCCCCCCCTCCGCCCCGTCGGAGAGCTGAGAAACTTGCTGGGAAGGTTCTGGGCTGAG
  GGTGGCCTCTTTTGGTTGGGCGCCGTTGGGGAGGGGCCGGGGCCGCTCTGAGCGCGGTCT
  CATGCCCCCGCCCCCTCCCCTGTCTTGTCTTCGCGGGCTCCAGGCTCCCCATCAACCCGG
  GCAGCCGGGATTTAAATTCACGGTGGCTGAGTCTTGTGACAGGATCAAAGACGAATTCCA
  GTTCCTGCAAGCTCAGTATCACAGCCTCAAAGTGGAGTACGACAAGCTGGCAAACGAGAA
  GACGGAGATGCAGCGCCATTATGTGATGTACTATGAGATGTCCTATGGCTTGAACATTGA
  AATGCACAAGCAGACAGAGATTGCGAAGAGACTGAACACAATTTTAGCACAGATCATGCC
  TTTCCTGTCACAAGAGCACCAGCAGCAGGTGGCGCAGGCAGTGGAGCGCGCCAAGCAGGT
  CACCATGACGGAGCTGAACGCCATCATCGGGCAGCAGCTCCAGGCGCAGCACCTCTCCCA
  TGCCACACACGGCCCCCCGGTCCAGTTGCCACCCCACCCGTCAGGTCTCCAGCCTCCAGG
  AATCCCCCCAGTGACAGGGAGCAGCTCCGGGCTGCTGGCACTGGGCGCCCTGGGCAGCCA
  GGCCCATCTGACGGTGAAGGATGAGAAGAACCACCATGAACTCGATCACAGAGAGAGAGA
  ATCCAGTGCGAATAACTCTGTGTCACCCTCGGAAAGCCTCCGGGCCAGTGAGAAGCACCG
  GGGCTCTGCGGACTACAGCATGGAAGCCAAGAAGCGGAAGGCGGAAGAGAAGGACAGCTT
  GAGCCGATACGACAGTGATGGGGACAAGAGTGATGATCTGGTGGTGGATGTTTCCAATGA
  GGACCCCGCAACGCCCCGGGTCAGCCCGGCACACTCCCCTCCTGAAAATGGGCTGGACAA
  GGCCCGTAGCCTGAAAAAAGATGCCCCCACCAGCCCTGCCTCGGTGGCCTCTTCCAGTAG
  CACACCTTCCTCCAAGACCAAAGACCTTGGTCATAACGACAAATCCTCCACCCCTGGGCT
  CAAGTCCAACACACCAACCCCAAGGAACGACGCCCCAACTCCAGGCACCAGCACGACCCC
  AGGGCTCAGGTCGATGCCGGGTAAACCTCCGGGCATGGACCCGATAGGTATAATGGCCTC
  GGCTCTGCGCACGCCCATCTCCATCACCAGCTCCTATGCGGCGCCCTTCGCCATGATGAG
  CCACCATGAGATGAACGGCTCCCTCACCAGTCCTGGCGCCTACGCCGGCCTCCACAACAT
  CCCACCCCAGATGAGCGCCGCCGCCGCTGCTGCAGCCGCTACCTATGGCCGATCGCCAAT
  GGTTGGTTTTGACCCTCACCCCCCGATGCGGGCCACAGGCCTCCCCTCAAGCCTGGCCTC
  CATTCCTGGAGGAAAACCAGCGTACTCATTCCATGTGAGTGCTGATGGGCAGATGCAGCC
  CGTGCCCTTCCCCCACGACGCCCTGGCAGGCCCCGGCATCCCGAGGCACGCCCGGCAGAT
  CAACACACTCAGCCACGGGGAGGTGGTGTGTGCCGTGACCATCAGCAACCCCACGAGGCA
  CGTCTACACAGGTGGCAAGGGCTGCGTGAAGATCTGGGACATCAGCCAGCCAGGCAGCAA
  GAGCCCCATCTCCCAGCTGGACTGCCTGAACAGGGACAATTACATCCGCTCCTGCAAGCT
  GCTCCCTGATGGGCGCACGCTCATCGTGGGCGGCGAGGCCAGCACGCTCACCATCTGGGA
  CCTGGCCTCACCCACGCCCCGCATCAAGGCCGAGCTGACGTCCTCGGCTCCCGCCTGTTA
  TGCCCTGGCCATTAGCCCTGACGCCAAAGTCTGCTTCTCCTGCTGCAGCGATGGGAACAT
  TGCTGTCTGGGACCTGCACAACCAGACCCTGGTCAGGCAGTTCCAGGGCCACACAGATGG
  GGCCAGCTGCATAGACATCTCCCATGATGGCACCAAACTGTGGACAGGGGGCCTGGACAA
  CACAGTGCGCTCCTGGGACCTGCGGGAGGGCCGACAGCTACAGCAGCATGACTTCACTTC
  CCAGATCTTCTCGCTGGGCTACTGCCCCACTGGGGAGTGGCTGGCTGTGGGCATGGAGAG
  CAGCAACGTGGAGGTGCTGCACCACACCAAGCCTGACAAGTACCAGCTGCACCTGCACGA
  GAGCTGCGTGCTCTCCCTCAAGTTCGCCTACTGCGGCAAGTGGTTCGTGAGCACTGGGAA
  AGATAACCTTCTCAACGCCTGGAGGACGCCTTATGGAGCCAGCATATTCCAGTCTAAAGA
  ATCCTCGTCTGTCTTGAGTTGTGACATTTCAGCGGATGACAAATACATTGTAACAGGCTC
  TGGTGACAAGAAGGCCACAGTTTATGAGGTCATCTACTAAACAAGAACTCCAGCAGGGCT
  GTCAAACTCTGGGAGAAACCGACTCGGCTCTGACAGGGAGACCCCCAGGCGAGGGGCCCC
  GAGGATGGCGGAGGATGGGCCGCAGGCAGCCGAGCGTTCAGGGCTGCGCTCCGGCCGGCT
  GAGAGGGCACGTGCCCCGTCACAGTCTGGACTCCTGGGCCTGGATTGATGTGTCTCACAG
  ACTCGGAAGGGTTCTGCTCCTCCTCCTCCCCCTGAACAATGCTGGCAGTTGCTACAAATA
  GATTTATTGGAGGCTTATGGCTCCGGTTCCCCCACAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC041831 unedited
  GAGTGCAGGTCAGGATTTTGTAATACGACTCACTATAGGCGGCCGCATAACTTCGTATAG
  CATACATTATACGAAGTTATGGATCAGGCCAAATCGGCCGAGCTCGAATTCGTCGAGAGC
  GGAATTCCGCGCGAGTGGCGCGGGGGGCTGCTTGTGTTCCAGGGGGAGCTGTTCACGCGG
  TCCCCGGCCCCGCTCTGAAGTCTCCCAACTCACCCGGCTTGTCTCCCGCCCCCGCCTTAC
  TGGGGAGAGGGAGGCCCTTGGTGAAAGGCATGGGAGCCCCGCTGCCGACGGGGAGAGATG
  GGGCGCCCCCCCTCCGCCCCGTCGGAGAGCTGAGAAACTTGCTGGGAAGGTTCTGGGCTG
  AGGGTGGCCTCTTTTGGTTGGGCCCGTTGGGGGAGATTATAATAAAAAGAGGAATAGAAC
  TGAGCCGGGGAGATGTGGACCCACAGTGTACAGTTAAAGAGAAGTATATCCACGTTGGCC
  AGGTTTGTGGGGTTGTTGAGCGTGGGAGACGGCACGGGCCCACCCGTTGTGTTTCGCTAC
  TTCGAACAGCCGACCACCGTGTGTCCGGTTGGCTCCCTATTTGCTTGAGTTTAGCCTCCT
  ATTTCCATTTGACCGGCACCCTTATCATTGTGATGGGTAGTGCCGCGGTGAACCCAATAC
  AACCAATGGGTTGTCAACAGTTTAGAAATGCGGAACGATTTGCCCTAAAACCCAACAAAC
  CATCTCAAAGAGCATTTGAGCCATGTAATGAAATAGTAGGAGGTGCAGGGTCAGTTATAC
  GAAGGGGCGACAAGATGATACACGCCCGAGTCTACCGACGTCACCACCCGGTAGCCACTC
  GGCGCTGTGTTGTTTATAGGGTGGATTAGCGCGCAAAAGCAGACTAATGT
  
• Restriction Sites: NotI-NotI
• ACCN: BC041831
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC041831.1, AAH41831.1
• RefSeq Size: 2931 bp
• Locus ID: 7090
• Cytogenetics: 15q23
• Protein Families: Transcription Factors
• Gene Summary: 'This gene encodes a transcriptional co-repressor protein that belongs to the transducin-like enhancer family of proteins. The members of this family function in the Notch signaling pathway that regulates determination of cell fate during development. Expression of this gene has been associated with a favorable outcome to chemotherapy with taxanes for ovarian carcinoma. Alternate splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Sep 2013]'