CHRNA3 (BC006114) Human Untagged Clone

CAT#: SC126406

CHRNA3 (untagged)-Human cholinergic receptor, nicotinic, alpha polypeptide 3, mRNA (cDNA clone MGC:12991 IMAGE:3503197), complete cds


Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol CHRNA3
Synonyms Cholinergic receptor, neuronal nicotinic, alpha polypeptide-3; cholinergic receptor, nicotinic, alpha 3; cholinergic receptor, nicotinic, alpha polypeptide 3; LNCR2; MGC104879; neuronal nicotinic acetylcholine receptor, alpha3 subunit; PAOD2
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for BC006114 edited
GTCCTTCCCAGGGTGTGTTAGCGTCTCTCCTACGGCGCCCGCCTAGCCCGGTGGTCCCAA
CCCCCTGCGGGAGCTGCACACGCCCCGGAAACCTGGGACAGAAACTGAGTCCCTCTCCTT
CCTGGTGGTGGTGACAGCACCTGCTCAGATCTGGTCGGACGCCGCCGGCCGGAGCACCCA
GCCCGGCGGAGAAGGAGCTCGCCCGGCGCTGGGGACTGGGACCTGGAGCCCCTTCCCCTA
CCGCACGTACGCCCCGCCCCGCGCACGCCCGCCCGCCCGCCCGCGCCTGGCGCAGCTTCA
CTCCGGATGGTTCCTGTCCTCCCGCGGGTCCGAGGGCGCTGGAAACCCAGCGGCGGCGAA
GCGGAGAGGAGCCCCGCGCGTCTCCGCCCGCACGGCTCCAGGTCTGGGGTCTGCGCTGGA
GCCGCGCGGGGAGAGGCCGTCTCTGCGACCGCCGCGCCCGCTCCCGACCGTCCGGGTCCG
CGGCCAGCCCGGCCACCAGCCATGGGCTCTGGCCCGCTCTCGCTGCCCCTGGCGCTGTCG
CCGCCGCGGCTGCTGCTGCTGCTGCTGCTGTCTCTGCTGCCAGTGGCCAGGGCCTCAGAG
GCTGAGCACCGTCTATTTGAGCGGCTGTTTGAAGATTACAATGAGATCATCCGGCCTGTA
GCCAACGTGTCTGACCCAGTCATCATCCATTTCGAGGTGTCCATGTCTCAGCTGGTGAAG
GTGGATGAAGTAAACCAGATCATGGAGACCAACCTGTGGCTCAAGCAAATCTGGAATGAC
TACAAGCTGAAATGGAACCCCTCTGACTATGGTGGGGCAGAGTTCATGCGTGTCCCTGCA
CAGAAGATCTGGAAGCCAGACATTGTGCTGTATAACAATGCTGTTGGGGATTTCCAGGTG
GACGACAAGACCAAAGCCTTACTCAAGTACACTGGGGAGGTGACTTGGATACCTCCGGCC
ATCTTTAAGAGCTCCTGTAAAATCGACGTGACCTACTTCCCGTTTGATTACCAAAACTGT
ACCATGAAGTTCGGTTCCTGGTCCTACGATAAGGCGAAAATCGATCTGGTCCTGATCGGC
TCTTCCATGAACCTCAAGGACTATTGGGAGAGCGGCGAGTGGGCCATCATCAAAGCCCCA
GGCTACAAACACGACATCAAGTACAACTGCTGCGAGGAGATCTACCCCGACATCACATAC
TCGCTGTACATCCGGCGCCTGCCCTTGTTCTACACCATCAACCTCATCATCCCCTGCCTG
CTCATCTCCTTCCTCACTGTGCTCGTCTTCTACCTGCCCTCCGACTGCGGTGAGAAGGTG
ACCCTGTGCATTTCTGTCCTCCTCTCCCTGACGGTGTTTCTCCTGGTGATCACTGAGACC
ATCCCTTCCACCTCGCTGGTCATCCCCCTGATTGGAGAGTACCTCCTGTTCACCATGATT
TTTGTAACCTTGTCCATCGTCATCACCGTCTTCGTGCTCAACGTGCACTACAGAACCCCG
ACGACACACACAATGCCCTCATGGGTGAAGACTGTATTCTTGAACCTGCTCCCCAGGGTC
ATGTTCATGACCAGGCCAACAAGCAACGAGGGCAACGCTCAGAAGCCGAGGCCCCTCTAC
GGTGCCGAGCTCTCAAATCTGAATTGCTTCAGCCGCGCAGAGTCCAAAGGCTGCAAGGAG
GGCTACCCCTGCCAGGACGGGATGTGTGGTTACTGCCACCACCGCAGGATAAAAATCTCC
AATTTCAGTGCTAACCTCACGAGAAGCTCTAGTTCTGAATCTGTTGATGCTGTGCTGTCC
CTCTCTGCTTTGTCACCAGAAATCAAAGAAGCCATCCAAAGTGTCAAGTATATTGCTGAA
AATATGAAAGCACAAAATGAAGCCAAAGAGGAACAAAAAGCCCAAGAGATCCAACAATTG
AAACGAAAAGAAAAGTCCACAGAAACATCCGATCAAGAACCTGGGCTATGAATTTCCAAT
CTTCAACAACCTGTTAGGAAAAAAATAAAAATGCATTAAATAATTGTAAAAAAAAAAAAA
AAAAAA
>OriGene 5' read for BC006114 unedited
NAAGGTCGNCATTTGTATACGACTCATATAGGCGGACCGCGAATTCGCACGAGGTCCTTC
CCAGGTGTGTTAGCGTCTCTCCTACGGCGCCCGCCTAGCCCGGTGGTCCCAACCCCCTGC
GGGAGCTGCACACGCCCCGGAAACCTGGGACAGAAACTGAGTCCCTCTCCTTCCTGGTGG
TGGTGACAGCACCTGCTCAGATCTGGTCGGACGCCGCCGGCCGGAGCACCCAGCCCGGCG
GAGAAGGAGCTCGCCCGGCGCTGGGGACTGGGACCTGGAGCCCCTTCCCCTACCGCACGT
ACGCCCCGCCCCGCGCACGCCCGCCCGCCCGCCCGCGCCTGGCGCAGCTTCACTCCGGAT
GGTTCCTGTCCTCCCGCGGGTCCGAGGGCGCTGGAAACCCAGCGGCGGCGAAGCGGAGAG
GAGCCCCGCGCGTCTCCGCCCGCACGGCTCCAGGTCTGGGGTCTGCGCTGGAGCCGCGCG
GGGAGAGGCCGTCTCTGCGACCGCCGCGCCCGCTCCCGACCGTCCGGGTCCGCGGCCAGC
CCGGCCACCAGCCATGGGCTCTGGCCCGCTCTCGCTGCCCCTGGCGCTGTCGCCGCCGCG
GCTGCTGCTGCTGCTGCTGCTGTCTCTGCTGCCAGTGGCCAGGGCCTCAGAGGCTGAGCA
CCGTCTATTTGAGCGGCTGTTTGAAGATTACAATGAGATCATCCGGCCTGTAGCCAACGT
GTCTGACCCAGTCATCATCCATTTCGAGGTGTCCATGTCTCAGCTGGTGAAGGTGGATGA
AGTAAACCAGATCATGGAGACCAACCTGTGGCTCAAGCAAATCTGGNAATGACTACAGCT
GAAATGGAACCCCTCTGACTATGGTGGGGCAGAGTTCATGCGTGTCCCTGCACAGAAGAT
CTGGAAGCCAGA
Restriction Sites Please inquire     
ACCN BC006114
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq BC006114.1, AAH06114.1
RefSeq Size 2046 bp
RefSeq ORF 1467 bp
Locus ID 1136
Cytogenetics 15q25.1
Protein Families Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane
Gene Summary 'This locus encodes a member of the nicotinic acetylcholine receptor family of proteins. Members of this family of proteins form pentameric complexes comprised of both alpha and beta subunits. This locus encodes an alpha-type subunit, as it contains characteristic adjacent cysteine residues. The encoded protein is a ligand-gated ion channel that likely plays a role in neurotransmission. Polymorphisms in this gene have been associated with an increased risk of smoking initiation and an increased susceptibility to lung cancer. Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov 2009]'

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