TPSAB1 (BC028059) Human Untagged Clone

CAT#: SC126411

TPSAB1 (untagged)-Human tryptase alpha/beta 1, mRNA (cDNA clone MGC:39869 IMAGE:5181815), complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TPSAB1
• Synonyms: lung tryptase; mast cell protease II; mast cell tryptase; MCP7; pituitary tryptase; skin tryptase; TPS1; TPS2; TPSB1; tryptase, alpha; tryptase-I; tryptase-III; tryptase 1; tryptase alpha/b; tryptase alpha II; tryptase beta 1; tryptase beta I; tryptase II
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for BC028059 edited
  GGAGTGGCCAGGATGCTGAGCCTGCTGCTGCTGGCGCTGCCCGTCCTGGCGAGCCCGGCC
  TACGCGGCCCCTGCCCCAGTCCAGGCCCTGCAGCAAGCGGGTATCGTCGGGGGTCAGGAG
  GCCCCCAGGAGCAAGTGGCCCTGGCAGGTGAGCCTGAGAGTCCGCGACCGATACTGGATG
  CACTTCTGTGGGGGCTCCCTCATCCACCCCCAGTGGGTGCTGACCGCGGCGCACTGCCTG
  GGACCGGACGTCAAGGATCTGGCCACCCTCAGGGTGCAACTGCGGGAGCAGCACCTCTAC
  TACCAGGACCAGCTGCTGCCGGTCAGCAGGATCATCGTGCACCCACAGTTCTACATCATC
  CAGACTGGAGCGGATATCGCCCTGCTGGAGCTGGAGGAGCCCGTGAACATCTCCAGCCGC
  GTCCACACGGTCATGCTGCCCCCTGCCTCGGAGACCTTCCCCCCGGGGATGCCGTGCTGG
  GTCACTGGCTGGGGCGATGTGGACAATGATGAGCCCCTCCCACCGCCATTTCCCCTGAAG
  CAGGTGAAGGTCCCCATAATGGAAAACCACATTTGTGACGCAAAATACCACCTTGGCGCC
  TACACGGGAGACGACGTCCGCATCATCCGTGACGACATGCTGTGTGCCGGGAACACCCGG
  AGGGACTCATGCCAGGGCGACTCTGGAGGGCCCCTGGTGTGCAAGGTGAATGGCACCTGG
  CTACAGGCGGGCGTGGTCAGCTGGGACGAGGGCTGTGCCCAGCCCAACCGGCCTGGCATC
  TACACCCGTGTCACCTACTACTTGGACTGGATCCACCACTATGTCCCCAAAAAGCCGTGA
  GTCAGGCCTGGGGTGTCCACCTGGGTCACTGGAGAGCCAGCCCCTCCTGTCCAAAACACC
  ACTGCTTCCTACCCAGGTGGCGACTGCCCCCCACACCTTCCCTGCCCCGTCCTGAGTGCC
  CCTTCCTGTCCTAAGCCCCCTGCTCTCTTCTGAGCCCCTTCCCCTGTCCTGAGGACCCTT
  CCCCATCCTGAGCCCCCTTCCCTGTCCTAAGCCTGACGCCTGCACTGGGCCCTCCGGCCC
  TCCCCTGCCCAGGCAGCTGGTGGTGGGCGCTAATCCTCCTGAGTGCTGGACCTCATTAAA
  GTGCATGGAAATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC028059 unedited
  CCCCGATCGGGATTTGTAATACGACTTTATATAGGCGGCCGCGNATTCANATCTGGTACC
  GGTCCGGAATTCCCGGGATGGAGTGGCCAGGATGCTGAGCCTGCTGCTGCTGGCGCTGCC
  CGTCCTGGCGAGCCCGGCCTACGCGGCCCCTGCCCCAGTCCAGGCCCTGCAGCAAGCGGG
  TATCGTCGGGGGTCAGGAGGCCCCCAGGAGCAAGTGGCCCTGGCAGGTGAGCCTGAGAGT
  CCGCGACCGATACTGGATGCACTTCTGTGGGGGCTCCCTCATCCACCCCCAGTGGGTGCT
  GACCGCGGCGCACTGCCTGGGACCGGACGTCAAGGATCTGGCCACCCTCAGGGTGCAACT
  GCGGGAGCAGCACCTCTACTACCAGGACCAGCTGCTGCCGGTCAGCAGGATCATCGTGCA
  CCCACAGTTCTACATCATCCAGACTGGAGCGGATATCGCCCTGCTGGAGCTGGAGGAGCC
  CGTGAACATCTCCAGCCGCGTCCACACGGTCATGCTGCCCCCTGCCTCGGAGACCTTCCC
  CCCGGGGATGCCGTGCTGGGTCACTGGCTGGGGCGATGTGGACAATGATGAGCCCCTCCC
  ACCGCCATTTCCCCTGAAGCAGGTGAAGGTCCCCATAATGGAAAACCACATTTGTGACGC
  AAAATACCACCTTGGCGCCTACACGGGAGACGACGTCCGCATCATCCGTGACGACATGCT
  GTGTGCCGGGAACACCCGGAGGGACTCATGCCAGGGCGACTCTGGAGGGCCCCTGGTGTG
  CAAGTGAATGGGACCTGGCTACAGGCGGGCGTGGTCAGCTGGGACGAGGCTGTGCCCAAC
  CCAACCGGCCTGGCATCTACACCCGGGTCACCTACTACCTGGGACTGTATCCCACCCT
  
• Restriction Sites: Please inquire
• ACCN: BC028059
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC028059.1, AAH28059.1
• RefSeq Size: 1206 bp
• Locus ID: 7177
• Cytogenetics: 16p13.3
• Protein Families: Druggable Genome, Protease, Secreted Protein
• Gene Summary: 'Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. These genes are characterized by several distinct features. They have a highly conserved 3' UTR and contain tandem repeat sequences at the 5' flank and 3' UTR which are thought to play a role in regulation of the mRNA stability. These genes have an intron immediately upstream of the initiator Met codon, which separates the site of transcription initiation from protein coding sequence. This feature is characteristic of tryptases but is unusual in other genes. The alleles of this gene exhibit an unusual amount of sequence variation, such that the alleles were once thought to represent two separate genes, alpha and beta 1. Beta tryptases appear to be the main isoenzymes expressed in mast cells; whereas in basophils, alpha tryptases predominate. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders. [provided by RefSeq, Jul 2008]'