NuMA (NUMA1) (BC013023) Human Untagged Clone

CAT#: SC126437

NUMA1 (untagged)-Human nuclear mitotic apparatus protein 1, mRNA (cDNA clone MGC:4620 IMAGE:3506457), complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NUMA1
• Synonyms: centrophilin stabilizes mitotic spindle in mitotic cells; nuclear mitotic apparatus protein 1; NUMA; structural nuclear protein
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for BC013023 edited
  GAAAAGATGGCTGCCACCAGCAAAGAGGTGGCCCGCTTGGAGACCTTGGTGCGCAAGGCA
  GGTGAGCAGCAGGAAACAGCCTCCCGGGAGTTAGTCAAGGAGCCTGCGAGGGCAGGAGAC
  AGACAGCCCGAGTGGCTGGAAGAGCAACAGGGACGCCAGTTCTGCAGCACACAGGCAGCG
  CTGCAGGCTATGGAGCGGGAGGCAGAGCAGATGGGCAATGAGCTGGAACGGCTGCGGGCC
  GCGCTGATGGAGAGCCAGGGGCAGCAGCAGGAGGAGCGTGGGCAGCAGGAAAGGGAGGTG
  GCGCGGCTGACCCAGGAGCGGGGCCGTGCCCAGGCTGACCTTGCCCTGGAGAAGGCGGCC
  AGAGCAGAGCTTGAGATGCGGCTGCAGAACGCCCTCAACGAGCAGCGTGTGGAGTTCGCT
  ACCCTGCAAGAGGCACTGGCTCATGCCCTGACGGAAAAGGAAGGCAAGGACCAGGAGTTG
  GCCAAGCTTCGTGGTCTGGAGGCAGCCCAGATAAAAGAGCTGGAGGAACTTCGGCAAACC
  GTGAAGCAACTGAAGGAACAGCTGGCTAAGAAAGAAAAGGAGCACGCATCTGGCTCAGGA
  GCCCAATCTGAGGCTGCTGGCAGGACAGAGCCAACAGGCCCCAAGCTGGAGGCACTGCGG
  GCAGAGGTGAGCAAGCTGGAACAGCAATGCCAGAAGCAGCAGGAGCAGGCTGACAGCCTG
  GAACGCAGCCTCGAGGCTGAGCGGGCCTCCCGGGCTGAGCGGGACAGTGCTCTGGAGACT
  CTGCAGGGCCAGTTAGAGGAGAAGGCCCAGGAGCTAGGGCACAGTCAGAGTGCCTTAGCC
  TCGGCCCAACGGGAGTTGGCTGCCTTCCGCACCAAGGTACAAGACCACAGCAAGGCTGAA
  GATGAGTGGAAGGCCCAGGTGGCCCGGGGCCGGCAAGAGGCTGAGAGGAAAAATAGCCTC
  ATCAGCAGCTTGGAGGAGGAGGTGTCCATCCTGAATCGCCAGGTCCTGGAGAAGGAGGGG
  GAGAGCAAGGAGTTGAAGCGGCTGGTGATGGCCGAGTCAGAGAAGAGCCAGAAGCTGGAG
  GAGAGGCTGCGCCTGCTGCAGGCAGAGACAGCCAGCAACAGTGCCAGAGCTGCAGAACGC
  AGCTCTGCTCTGCGGGAGGAGGTGCAGAGCCTCCGGGAGGAGGCTGAGAAACAGCGGGTG
  GCTTCAGAGAACCTGCGGCAGGAGCTGACCTCACAGGCTGAGCGTGCGGAGGAGCTGGGC
  CAAGAATTGAAGGCGTGGCAGGAGAAGTTCTTCCAGAAAGAGCAGGCCCTCTCCACCCTG
  CAGCTCGAGCACACCAGCACACAGGCCCTGGTGAGTGAGCTGCTGCCAGCTAAGCACCTC
  TGCCAGCAGCTGCAGGCCGAGCAGGCCGCTGCCGAGAAACGCCACCGTGAGGAGCTGGAG
  CAGAGCAAGCAGGCCGCTGGGGCACTGCGGGCAGAGCTGCTGCGGGCCCAGCGGGAGCTT
  GGGGAGCTGATTCCTCTGCGGCAGAAGGTGGCAGAGCAGGAGCGAACAGCTCAGCAGCTG
  CGGGCAGAGAAGGCCAGCTATGCAGAGCAGCTGAGCATGCTGAAGAAGGCGCATGGCCTG
  CTGGCAGAGGAGAACCGGGGGCTGGGTGAGCGGGCCAACCTTGGCCGGCAGTTTCTGGAA
  GTGGAGTTGGACCAGGCCCGGGAGAAGTATGTCCAAGAGTTGGCAGCCGTACGTGCTGAT
  GCTGAGACCCGTCTGGCTGAGGTGCAGCGAGAAGCACAGAGCACTGCCCGGGAGCTGGAG
  GTGATGACTGCCAAGTATGAGGGTGCCAAGGTCAAGGTCCTGGAGGAGAGGCAGCGGTTC
  CAGGAAGAGAGGCAGAAACTCACTGCCCAGGTGGAAGAACTGAGTAAGAAACTGGCTGAC
  TCTGACCAAGCCAGCAAGGTGCAGCAGCAGAAGCTGAAGGCTGTCCAGGCTCAGGGAGGC
  GAGAGCCAGCAGGAGGCCCAGCGCCTCCAGGCCCAGCTGAATGAACTGCAAGCCCAGTTG
  AGCCAGAAGGAGCAGGCAGCTGAGCACTATAAGCTGCAGATGGAGAAAGCCAAAACACAT
  TATGATGCCAAGAAGCAGCAGAACCAAGAGCTGCAGGAGCAGCTGCGGAGCCTGGAGCAG
  CTGCAGAAGGAAAACAAAGAGCTGCGAGCTGAAGCTGAACGGCTGGGCCATGAGCTACAG
  CAGGCTGGGCTGAAGACCAAGGAGGCTGAACAGACCTGCCGCCACCTTACTGCCCAGGTG
  CGCAGCCTGGAGGCACAGGTTGCCCATGCAGACCAGCAGCTTCGAGACCTGGGCAAATTC
  CAGGTGGCAACTGATGCTTTAAAGAGCCGTGAGCCCCAGGCTAAGCCCCAGCTGGACTTG
  AGTATTGACAGCCTGGATCTGAGCTGCGAGGAGGGGACCCCACTCAGTATCACCAGCAAG
  CTGCCTCGTACCCAGCCAGACGGCACCAGCGTCCCTGGAGAACCAGCCTCACCTATCTCC
  CAGCGCCTGCCCCCCAAGGTAGAATCCCTGGAGAGTCTCTACTTCACTCCCATCCCTGCT
  CGGAGTCAGGCCCCCCTGGAGAGCAGCCTGGACTCCCTGGGAGACGTCTTCCTGGACTCG
  GGTCGTAAGACCCGCTCCGCTCGTCGGCGCACCACGCAGATCATCAACATCACCATGACC
  AAGAAGCTAGATGTGGAAGAGCCAGACAGCGCCAACTCATCGTTCTACAGCACGCGGTCT
  GCTCCTGCTTCCCAGGCTAGCCTGCGAGCCACCTCCTCTACTCAGTCTCTAGCTCGCCTG
  GGTTCTCCCGATTATGGCAACTCAGCCCTGCTCAGCTTGCCTGGCTACCGCCCCACCACT
  CGCAGTTCTGCTCGTCGTTCCCAGGCCGGGGTGTCCAGTGGGGCCCCTCCAGGAAGGAAC
  AGCTTCTACATGGGCACTTGCCAGGATGAGCCTGAGCAGCTGGATGACTGGAACCGCATT
  GCAGAGCTGCAGCAGCGCAATCGAGTGTGCCCCCCACATCTGAAGACCTGCTATCCCCTG
  GAGTCCAGGCCTTCCCTGAGCCTGGGCACCATCACAGATGAGGAGATGAAAACTGGAGAC
  CCCCAAGAGACCCTGCGCCGAGCCAGCATGCAGCCAATCCAGATAGCCGAGGGCACTGGC
  ATCACCACCCGGCAGCAGCGCAAACGGGTCTCCCTAGAGCCCCACCAGGGCCCTGGAACT
  CCTGAGTCTAAGAAGGCCACCAGCTGTTTCCCACGCCCCATGACTCCCCGAGACCGACAT
  GAAGGGCGCAAACAGAGCACTACTGAGGCCCAGAAGAAAGCAGCTCCAGCTTCTACTAAA
  CAGGCTGACCGGCGCCAGTCGATGGCCTTCAGCATCCTCAACACACCCAAGAAGCTAGGG
  AACAGCCTTCTGCGGCGGGGAGCCTCAAAGAAGGCCCTGTCCAAGGCTTCCCCCAACACT
  CGCAGTGGAACCCGCCGTTCTCCGCGCATTGCCACCACCACAGCCAGCGCCGCCACTGCT
  GCCGCCATTGGTGCCACCCCTCGAGCCAAGGGCAAGGCAAAGCACTAAAGGGCCAGTACC
  AGTGAGTGGCCCCACCTGTGTCCCCGATGCTGCCGTCACCTGGTCCTCCGCCTACTGTCC
  CTCTCAGTGCCTTCTCTCAGCTCCCAGGCCAACAGTAGCCAAACCCCTAGAGACAGTGAT
  GCCTGCCCGCACCCTGGCCTGGTCCCTGGTCCTTCACTGGCGCCTTCTCGGAGCTGGCCC
  AGGGGGCCTGGAGCATGGACAGTGTGGGCGCTCTCCCTACCTTGCCTCCTTTTTTCTTAA
  AGCAAAGTCACTTCTCCATCACAACCAGATTTGAGGCTGGTTTTGATGGCTGGGTCCTTG
  GGCCTGGCCAGTCTTCCTCTTAGCCTCTGGATCTAGAAGGGACCATAAGAGGAGTAGGCC
  CTGGTTCCTGCTGTCCTGGTGGCTGGGCCCAGCAGGGGCCCTCACTCTTGAAGTCCAGGA
  CTGGGTCTGACCTGGTGGGAGCACCTGCCAGAGGATGCTCTTTCCCAGGACGGATGGGCC
  CTGTGTCTCAGGAGTGGGGTTGGGGGACAGCCTTCAGCAGCAGCTCACACCCTACCTTCC
  CCAGACTTGCACTGGGGTGGGATTTGGAGTGATGGGAAGGTTTTTAAGGGCCGGGGATGG
  ATCTTTTCTAAATGTTATTACTTGTAAATAAAGTCTATTTTTCTCCCGTGAAAAAAAAAA
  AAAAAACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC013023 unedited
  GGTTCATAATTTGTATACGACNACTATAGGCGGCCGCGTAATTCGCACGAGGGAAAAAGA
  GGCTGCCACCAGCAAAGAGTGGCCCGCTTGGAGACCTTGGTGCGCAAGGCAGGTGAGCAG
  CAGGAAACAGCCTCCCGGGAGTTAGTCAAGGAGCCTGCGAGGGCAGGAGACAGACAGCCC
  GAGTGGCTGGAAGAGCAACAGGGACGCCAGTTCTGCAGCACACAGGCAGCGCTGCAGGCT
  ATGGAGCGGGAGGCAGAGCAGATGGGCAATGAGCTGGAACGGCTGCGGGCCGCGCTGATG
  GAGAGCCAGGGGCAGCAGCAGGAGGAGCGTGGGCAGCAGGAAAGGGAGGTGGCGCGGCTG
  ACCCAGGAGCGGGGCCGTGCCCAGGCTGACCTTGCCCTGGAGAAGGCGGCCAGAGCAGAG
  CTTGAGATGCGGCTGCAGAACGCCCTCAACGAGCAGCGTGTGGAGTTCGCTACCCTGCAA
  GAGGCACTGGCTCATGCCCTGACGGAAAAGGAAGGCAAGGACCAGGAGTTGGCCAAGCTT
  CGTGGTCTGGAGGCAGCCCAGATAAAAGAGCTGGAGGAACTTCGGCAAACCGTGAAGCAA
  CTGAAGGAACAGCTGGCTAAGAAAGAAAAGGAGCACGCATCTGGCTCAGGAGCCCAATCT
  GAGGCTGCTGGCAGGACAGAGCCAACAGGCCCCAAGCTGGAGGCACTGCGGGCAGAGGTG
  AGCAAGCTGGAAACAGCATGCCAGAAGCAGCAGGAGCAGGCTGACAGCCTGGAACGCAGC
  CTCGACTCTAGATTGCGGCCGCGGTCATAGCTGTTTCCCTGACAGATCCCGGGTGGCATC
  CCTGTGACCCCTCCCCAGTGCCTCTCCCTGCCCTGGAAGTTTGCANNCTCAGTGCCACCA
  GCCTTGTCCTAATAAAATAAGTGCATCATTTTGTCTGACTAGGTGTT
  
• Restriction Sites: NotI-NotI
• ACCN: BC013023
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC013023.1, AAH13023.1
• RefSeq Size: 4322 bp
• Locus ID: 4926
• Cytogenetics: 11q13.4
• Gene Summary: 'This gene encodes a large protein that forms a structural component of the nuclear matrix. The encoded protein interacts with microtubules and plays a role in the formation and organization of the mitotic spindle during cell division. Chromosomal translocation of this gene with the RARA (retinoic acid receptor, alpha) gene on chromosome 17 have been detected in patients with acute promyelocytic leukemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2013]'