SPG7 (NM_003119) Human Untagged Clone

CAT#: SC126532

SPG7 (untagged)-Human spastic paraplegia 7 (pure and complicated autosomal recessive) (SPG7), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SPG7
• Synonyms: CAR; CMAR; PGN; SPG5C
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_003119, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCCGTGCTGCTGCTGCTGCTCCGTGCCCTCCGCCGGGGTCCAGGCCCGGGTCCTCGGCCGCTGTGGG
  GCCCAGGCCCGGCCTGGAGTCCAGGGTTCCCCGCCAGGCCCGGGAGGGGGCGGCCGTACATGGCCAGCAG
  GCCTCCGGGGGACCTCGCCGAGGCTGGAGGCCGAGCTCTGCAGAGCTTACAATTGAGACTGCTAACCCCT
  ACCTTTGAAGGGATCAACGGATTGTTGTTGAAACAACATTTAGTTCAGAATCCAGTCAGACTCTGGCAAC
  TTTTAGGTGGTACTTTCTATTTTAACACCTCAAGGTTGAAGCAGAAGAATAAGGAGAAGGATAAGTCGAA
  GGGGAAGGCGCCTGAAGAGGACGAAGAGGAGAGGAGACGCCGTGAGCGGGACGACCAGATGTACCGAGAG
  CGGCTGCGCACCTTGCTGGTCATCGCGGTTGTCATGAGCCTCCTGAATGCTCTCAGCACCAGCGGAGGCA
  GCATTTCCTGGAACGACTTTGTCCACGAGATGCTGGCCAAGGGCGAGGTGCAGCGCGTCCAGGTGGTGCC
  TGAGAGCGACGTGGTGGAAGTCTACCTGCACCCTGGAGCCGTGGTGTTTGGGCGGCCTCGGCTAGCCTTG
  ATGTACCGAATGCAGGTTGCAAATATTGACAAGTTTGAAGAGAAGCTTCGAGCAGCTGAAGATGAGCTGA
  ATATCGAGGCCAAGGACAGGATCCCAGTTTCCTACAAGCGAACAGGATTCTTTGGAAATGCCCTGTACTC
  TGTGGGGATGACGGCAGTGGGCCTGGCCATCCTGTGGTATGTTTTCCGTCTGGCCGGGATGACTGGAAGG
  GAAGGTGGATTCAGTGCTTTTAATCAGCTTAAAATGGCTCGTTTCACCATTGTGGATGGGAAGATGGGGA
  AAGGAGTCAGCTTCAAAGACGTGGCAGGAATGCACGAAGCCAAACTGGAAGTCCGCGAGTTTGTGGATTA
  TCTGAAGAGCCCAGAACGCTTCCTCCAGCTTGGCGCCAAGGTCCCAAAGGGCGCACTGCTGCTCGGCCCC
  CCCGGCTGTGGGAAGACGCTGCTGGCCAAGGCGGTGGCCACGGAGGCTCAGGTGCCCTTCCTGGCGATGG
  CCGGCCCAGAGTTCGTGGAGGTCATTGGAGGCCTCGGCGCTGCCCGTGTGCGGAGCCTCTTTAAGGAAGC
  CCGAGCCCGGGCCCCCTGCATCGTCTACATCGATGAGATCGACGCGGTGGGCAAGAAGCGCTCCACCACC
  ATGTCCGGCTTCTCCAACACGGAGGAGGAGCAGACGCTCAACCAGCTTCTGGTAGAAATGGATGGAATGG
  GTACCACAGACCATGTCATCGTCCTGGCGTCCACGAACCGAGCTGACATTTTGGACGGTGCTCTGATGAG
  GCCAGGCCGACTGGACCGGCACGTCTTCATTGATCTCCCCACGCTGCAGGAGAGGCGGGAGATTTTTGAG
  CAGCACCTGAAGAGCCTGAAGCTGACCCAGTCCAGCACCTTTTACTCCCAGCGTCTGGCAGAGCTGACAC
  CAGGATTCAGTGGGGCTGACATCGCCAACATCTGCAATGAGGCTGCGCTGCACGCGGCGCGGGAGGGACA
  CACTTCCGTGCACACTCTCAACTTCGAGTACGCCGTGGAGCGCGTCCTCGCAGGGACTGCCAAAAAGAGC
  AAGATCCTGTCCAAGGAAGAACAGAAAGTGGTTGCGTTTCATGAGTCGGGCCACGCCTTGGTGGGCTGGA
  TGCTGGAGCACACGGAGGCCGTGATGAAGGTCTCCATAACCCCTCGGACAAACGCCGCCCTGGGCTTTGC
  TCAGATGCTCCCCAGAGACCAGCACCTCTTCACCAAGGAGCAGCTGTTTGAGCGGATGTGCATGGCCCTG
  GGAGGACGGGCCTCGGAAGCACTGTCCTTCAACGAGGTCACTTCTGGGGCACAGGACGACCTGAGGAAGG
  TCACCCGCATCGCCTACTCCATGGTGAAGCAGTTTGGGATGGCACCTGGCATCGGGCCCATCTCCTTCCC
  TGAGGCGCAGGAGGGCCTCATGGGCATCGGGCGGCGCCCCTTCAGCCAAGGCCTGCAGCAGATGATGGAC
  CATGAAGCAAGACTGCTGGTGGCCAAGGCCTACAGACACACCGAGAAGGTGCTGCAGGACAACCTGGACA
  AGTTGCAGGCGCTGGCAAACGCCCTTCTGGAAAAGGAAGTGATAAACTATGAGGACATTGAGGCTCTCAT
  TGGCCCGCCGCCCCATGGGCCGAAGAAAATGATCGCACCGCAGAGGTGGATCGACGCCCAGAGGGAGAAA
  CAGGACTTGGGCGAGGAGGAGACCGAAGAGACCCAGCAGCCTCCACTTGGAGGCGAAGAGCCGACTTGGC
  CCAAGTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003119 unedited
  GCATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGTGGCCGTGCTGCTG
  CTGCTGCTCCGTGCCCTCCGCCGGGGTCCAGGCCCGGGTCCTCGGCCGCTGTGGGGCCCA
  GGCCCGGCCTGGAGTCCAGGGTTCCCCGCCAGGCCCGGGAGGGGGCGGCCGTACATGGCC
  AGCAGGCCTCCGGGGGACCTCGCCGAGGCTGGAGGCCGAGCTCTGCAGAGCTTACAATTG
  AGACTGCTAACCCCTACCTTTGAAGGGATCAACGGATTGTTGTTGAAACAACATTTAGTT
  CAGAATCCAGTCAGACTCTGGCAACTTTTAGGTGGTACTTTCTATTTTAACACCTCAAGG
  TTGAAGCAGAAGAATAAGGAGAAGGATAAGTCGAAGGGGAAGGCGCCTGAAGAGGACGAA
  GAGGAGAGGAGACGCCGTGAGCGGGACGACCAGATGTACCGAGAGCGGCTGCGCACCTTG
  CTGGTCATCGCGGTTGTCATGAGCCTCCTGAATGCTCTCAGCACCAGCGGAGGCAGCATT
  TCCTGGAACGACTTTGTCCACGAGATGCTGGCCAAGGGCGAGGTGCAGCGCGTCCAGGTG
  GTGCCTGAGAGCGACGTGGTGGAAGTCTACCTGCACCCTGNAGCCGTGGTGTTTGGGCGG
  CCTCGGCTAGCCTTGATGTACCGAATGCANGTTGCAAATATTGACAAGTTTGAAGAGAAG
  CTTCGAGCAGCTGAAGATGAGCTGAATATCGAGGCCAAGGACAGGATCCCCAGTTTCCTA
  CAAGCGAACAGGATTCTTTGGNAATGCCCTGTACTCTGTGGGGGATGACNGGCAGTGGGC
  CTGGCCATNCTGTGGGTATGGTTTTTCGTCTGGCCCGGATAACTGGAAAGGGAAGGGGGG
  AATCAATTGCCTTTAATCAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003119
• Insert Size: 3000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003119.2, NP_003110.1
• RefSeq Size: 3096 bp
• RefSeq ORF: 2388 bp
• Locus ID: 6687
• Cytogenetics: 16q24.3
• Domains: Peptidase_M41, AAA, AAA
• Protein Families: Protease, Transmembrane
• Gene Summary: 'This gene encodes a mitochondrial metalloprotease protein that is a member of the AAA family. Members of this protein family share an ATPase domain and have roles in diverse cellular processes including membrane trafficking, intracellular motility, organelle biogenesis, protein folding, and proteolysis. Mutations in this gene cause autosomal recessive spastic paraplegia 7. Two transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Mar 2014]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).