PKC alpha (PRKCA) (NM_002737) Human Untagged Clone

CAT#: SC126553

PRKCA (untagged)-Human protein kinase C, alpha (PRKCA)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PRKCA
• Synonyms: AAG6; PKC-alpha; PKCA; PKCalpha; PKCI+/-; PRKACA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_002737, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCTGACGTTTTCCCGGGCAACGACTCCACGGCGTCTCAGGACGTGGCCAACCGCTTCGCCCGCAAAG
  GGGCGCTGAGGCAGAAGAACGTGCACGAGGTGAAGGACCACAAATTCATCGCGCGCTTCTTCAAGCAGCC
  CACCTTCTGCAGCCACTGCACCGACTTCATCTGGGGGTTTGGGAAACAAGGCTTCCAGTGCCAAGTTTGC
  TGTTTTGTGGTCCACAAGAGGTGCCATGAATTTGTTACTTTTTCTTGTCCGGGTGCGGATAAGGGACCCG
  ACACTGATGACCCCAGGAGCAAGCACAAGTTCAAAATCCACACTTACGGAAGCCCCACCTTCTGCGATCA
  CTGTGGGTCACTGCTCTATGGACTTATCCATCAAGGGATGAAATGTGACACCTGCGATATGAACGTTCAC
  AAGCAATGCGTCATCAATGTCCCCAGCCTCTGCGGAATGGATCACACTGAGAAGAGGGGGCGGATTTACC
  TAAAGGCTGAGGTTGCTGATGAAAAGCTCCATGTCACAGTACGAGATGCAAAAAATCTAATCCCTATGGA
  TCCAAACGGGCTTTCAGATCCTTATGTGAAGCTGAAACTTATTCCTGATCCCAAGAATGAAAGCAAGCAA
  AAAACCAAAACCATCCGCTCCACACTAAATCCGCAGTGGAATGAGTCCTTTACATTCAAATTGAAACCTT
  CAGACAAAGACCGACGACTGTCTGTAGAAATCTGGGACTGGGATCGAACAACAAGGAATGACTTCATGGG
  ATCCCTTTCCTTTGGAGTTTCGGAGCTGATGAAGATGCCGGCCAGTGGATGGTACAAGTTGCTTAACCAA
  GAAGAAGGTGAGTACTACAACGTACCCATTCCGGAAGGGGACGAGGAAGGAAACATGGAACTCAGGCAGA
  AATTCGAGAAAGCCAAACTTGGCCCTGCTGGCAACAAAGTCATCAGTCCCTCTGAAGACAGGAAACAACC
  TTCCAACAACCTTGACCGAGTGAAACTCACGGACTTCAATTTCCTCATGGTGTTGGGAAAGGGGAGTTTT
  GGAAAGGTGATGCTTGCCGACAGGAAGGGCACAGAAGAACTGTATGCAATCAAAATCCTGAAGAAGGATG
  TGGTGATTCAGGATGATGACGTGGAGTGCACCATGGTAGAAAAGCGAGTCTTGGCCCTGCTTGACAAACC
  CCCGTTCTTGACGCAGCTGCACTCCTGCTTCCAGACAGTGGATCGGCTGTACTTCGTCATGGAATATGTC
  AACGGTGGGGACCTCATGTACCACATTCAGCAAGTAGGAAAATTTAAGGAACCACAAGCAGTATTCTATG
  CGGCAGAGATTTCCATCGGATTGTTCTTTCTTCATAAAAGAGGAATCATTTATAGGGATCTGAAGTTAGA
  TAACGTCATGTTGGATTCAGAAGGACATATCAAAATTGCTGACTTTGGGATGTGCAAGGAACACATGATG
  GATGGAGTCACGACCAGGACCTTCTGTGGGACTCCAGATTATATCGCCCCAGAGATAATCGCTTATCAGC
  CGTATGGAAAATCTGTGGACTGGTGGGCCTATGGCGTCCTGTTGTATGAAATGCTTGCCGGGCAGCCTCC
  ATTTGATGGTGAAGATGAAGACGAGCTATTTCAGTCTATCATGGAGCACAACGTTTCCTATCCAAAATCC
  TTGTCCAAGGAGGCTGTTTCTATCTGCAAAGGACTGATGACCAAACACCCAGCCAAGCGGCTGGGCTGTG
  GGCCTGAGGGGGAGAGGGACGTGAGAGAGCATGCCTTCTTCCGGAGGATCGACTGGGAAAAACTGGAGAA
  CAGGGAGATCCAGCCACCATTCAAGCCCAAAGTGTGTGGCAAAGGAGCAGAGAACTTTGACAAGTTCTTC
  ACACGAGGACAGCCCGTCTTAACACCACCTGATCAGCTGGTTATTGCTAACATAGACCAGTCTGATTTTG
  AAGGGTTCTCGTATGTCAACCCCCAGTTTGTGCACCCCATCTTACAGAGTGCAGTATGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002737 unedited
  TTACGCGCCCGTTGCCGCTTAGGGCGGTAGGCGTGTACGGTGGGNAGGTCTATATAAGCA
  GAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGA
  ATTCGGCACGAGGCGGCTCCGGCTCCCGCTCCCGCTCCGCGCAGCACCAGCCCGACTCTC
  CCCGGCCCCCGCCGCGCCCCCTCGCCGCGACCTCGGCCACCGGCCCGCGCCCCGCGCCCG
  GGGTCGCCCCGAGCCCGCACCTCTCCCCCGCCGCCCCCGCCCACCCGGCCCTCCGCGGCC
  GCAGCTCCCCGGCGGAGGCAAGAGGTGGTTGGGGGGGACCATGGCTGACGTTTTCCCGGG
  CAACGACTCCACGGCGTCTCAGGACGTGGCCAACCGCTTCGCCCGCAAAGGGGCGCTGAG
  GCAGAAGAACGTGCACGAGGTGAAGGACCACAAATTCATCGCGCGCTTCTTCAAGCAGCC
  CACCTTCTGCAGCCACTGCACCGACTTCATCTGGGGGTTTGGGAAACAAGGCTTCCAGTG
  CCAAGTTTGCTGTTTTGTGGTCCACAAGAGGTGCCATGAATTTGTTACTTTTTCTTGTCC
  GGGTGCGGATAATGGACCCGACACTGATGACCCCAGGAGCAAGCACAAGTTCAAAATCCA
  CACTTACGGAAGCCCCACCTTCTGCGATCACTGTGGGTCACTGCTCTATGGACTTATCCA
  TCAAGGGATGAAATGTGACACCTGCGATATGAACGTTCACAAGCAATGCGTCATCAATGT
  CCCCAGCCTCTGCGGAATGGATCACACTGAGAAGAAGGGGCGGGATTTACCTAAGGCTGA
  AGTTGCTGATGAAAGCTCCATGTCACAGTACGAGATGCAAAAAATCTATCCTATGATCCC
  CTTNCTTGAAGTTTCGGGCTGATAANAAGCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002737 unedited
  TGCACTTCCAGGCCAGGAGAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGATCTG
  TTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTGTTTTTT
  GTTATTAAGGTTTATTATAAACATGCAAAATTCATTGCATGATGCATTTGCAAAACGTAT
  TAAAATCCTTCTATTAACAAAACATCTTTGAGCTGTTAGGCATCCGGGTTTCCTGATTCT
  ACAGCTCCAGGCCTGTCATTCAACGTGAACTAGAAATGGGAACCAAAACCATATTCTTTT
  TATCCCTAAGTGGACATCCAGGGCTAGAGAAGGGAGGAAAATCAATCGGTAGAGGCTGAT
  GCTAGAGTCAGCACTTCCATTAAGTTACTGTACATCGTACTTTCATAAGCGACACTGCAG
  CCTATTAAATAAATAAGCACAAAAGGGGAGAGGGCGTACACTTCAGCTTCTTGAGAAGGA
  TAACAAGGTAGGCAGCAAAATGTTGGTGTGTGTGGGGGTTGGTGTTNGCNAACAACACTG
  GTGAGTAGTGGCATGATTACTGATGGCACCCTGGATGGAAAAATAATCTCCCAGAGATTA
  AAATGAACATATGTACACACTGGCTTATCCTTGATCTGAAATGATTTCACACGCTTGTTC
  AACAACGTCCTTAGACGATTTTGGCCTCTTACAAAGAATGAAAAGTATCAAGACGGTTAT
  CAACCCAGGAAGGTACCAGATGACAGATTCAC
  
• Restriction Sites: Please inquire
• ACCN: NM_002737
• Insert Size: 3200 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002737.2, NP_002728.1
• RefSeq Size: 8787 bp
• RefSeq ORF: 2019 bp
• Locus ID: 5578
• Cytogenetics: 17q24.2
• Domains: C2, pkinase, S_TK_X, TyrKc, DAG_PE-bind, S_TKc
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase
• Protein Pathways: Calcium signaling pathway, ErbB signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Gap junction, Glioma, GnRH signaling pathway, Leukocyte transendothelial migration, Long-term depression, Long-term potentiation, MAPK signaling pathway, Melanogenesis, Natural killer cell mediated cytotoxicity, Non-small cell lung cancer, Pathogenic Escherichia coli infection, Pathways in cancer, Phosphatidylinositol signaling system, Tight junction, Vascular smooth muscle contraction, VEGF signaling pathway, Vibrio cholerae infection, Wnt signaling pathway
• Gene Summary: 'Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes. [provided by RefSeq, Jul 2008]'