PPM1G (NM_177983) Human Untagged Clone

CAT#: SC126605

PPM1G (untagged)-Human protein phosphatase, Mg2+/Mn2+ dependent, 1G (PPM1G)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PPM1G
• Synonyms: PP2CG; PP2CGAMMA; PPP2CG
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_177983, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGTGCCTACCTCTCCCAGCCCAACACGGTGAAGTGCTCCGGGGACGGGGTCGGCGCCCCGCGCCTGC
  CGCTGCCCTACGGCTTCTCCGCCATGCAAGGCTGGCGCGTCTCCATGGAGGATGCTCACAACTGTATTCC
  TGAGCTGGACAGTGAGACAGCCATGTTTTCTGTCTACGATGGACATGGAGGGGAGGAAGTTGCCTTGTAC
  TGTGCCAAATATCTTCCTGATATCATCAAAGATCAGAAGGCCTACAAGGAAGGCAAGCTACAGAAGGCTT
  TAGAAGATGCCTTCTTGGCTATTGACGCCAAATTGACCACTGAAGAAGTCATTAAAGAGCTGGCACAGAT
  TGCAGGGCGACCCACTGAGGATGAAGATGAAAAAGAAAAAGTAGCTGATGAAGATGATGTGGACAATGAG
  GAGGCTGCACTGCTGCATGAAGAGGCTACCATGACTATTGAAGAGCTGCTGACACGCTACGGGCAGAACT
  GTCACAAGGGCCCTCCCCACAGCAAATCTGGAGGTGGGACAGGCGAGGAACCAGGGTCCCAGGGCCTCAA
  TGGGGAGGCAGGACCTGAGGACTCAACTAGGGAAACTCCTTCACAAGAAAATGGCCCCACAGCCAAGGCC
  TACACAGGCTTTTCCTCCAACTCGGAACGTGGGACTGAGGCAGGCCAAGTTGGTGAGCCTGGCATTCCCA
  CTGGTGAGGCTGGGCCTTCCTGCTCTTCAGCCTCTGACAAGCTGCCTCGAGTTGCTAAGTCCAAGTTCTT
  TGAGGACAGTGAGGATGAGTCAGATGAGGCGGAGGAAGAAGAGGAAGACAGTGAGGAATGCAGCGAGGAA
  GAGGATGGCTACAGCAGTGAGGAGGCAGAGAATGAGGAAGATGAGGATGACACCGAGGAGGCTGAAGAGG
  ACGATGAAGAAGAAGAAGAAGAGATGATGGTGCCAGGGATGGAAGGCAAAGAGGAGCCTGGCTCTGACAG
  TGGTACAACAGCGGTGGTGGCCCTGATACGAGGGAAGCAGTTGATTGTAGCCAACGCAGGAGACTCTCGC
  TGTGTGGTATCTGAGGCTGGCAAAGCTTTAGACATGTCCTATGATCACAAACCAGAGGATGAAGTAGAAC
  TAGCACGCATCAAGAATGCTGGTGGCAAGGTCACCATGGATGGGCGAGTCAACGGGGGCCTCAACCTCTC
  CAGAGCCATTGGGGACCACTTCTATAAGAGAAACAAGAACCTGCCACCTGAGGAACAGATGATTTCAGCC
  CTTCCTGACATCAAGGTGCTGACTCTCACTGACGACCATGAATTCATGGTCATTGCCTGTGATGGCATCT
  GGAATGTGATGAGCAGCCAGGAAGTTGTAGATTTCATTCAATCAAAGATCAGCCAGCGTGATGAAAATGG
  GGAGCTTCGGTTATTGTCATCCATTGTGGAAGAGCTGCTGGATCAGTGCCTGGCACCAGACACTTCTGGG
  GATGGTACAGGGTGTGACAACATGACCTGCATCATCATTTGCTTCAAGCCCCGAAACACAGCAGAGCTCC
  AGCCAGAGAGTGGCAAGCGAAAACTAGAGGAGGTGCTCTCTACTGAGGGGGCTGAAGAAAATGGCAACAG
  CGACAAGAAGAAGAAGGCCAAGCGAGACTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_177983 unedited
  GGATTTGTACACGACTCACTATAGGCGGCCGCGCAATTCGCACGAGGCCTGCACCTGAGG
  CCGCCGGCCAGCCGCCGCCATGGGTGCCTACCTCTCCCAGCCCAACACGGTGAAGTGCTC
  CGGGGACGGGGTCGGCGCCCCGCGCCTGCCGCTGCCCTACGGCTTCTCCGCCATGCAAGG
  CTGGCGCGTCTCCATGGAGGATGCTCACAACTGTATTCCTGAGCTGGACAGTGAGACAGC
  CATGTTTTCTGTCTACGATGGACATGGAGGGGAGGAAGTTGCCTTGTACTGTGCCAAATA
  TCTTCCTGATATCATCAAAGATCAGAAGGCCTACAAGGAAGGCAAGCTACAGAAGGCTTT
  AGAAGATGCCTTCTTGGCTATTGACGCCAAATTGACCACTGAAGAAGTCATTAAAGAGCT
  GGCACAGATTGCAGGGCGACCCACTGAGGATGAAGATGAAAAAGAAAAAGTAGCTGATGA
  AGATGATGTGGACAATGAGGAGGCTGCACTGCTGCATGAAGAGGCTACCATGACTATTGA
  AGAGCTGCTGACACGCTACGGGCAGAACTGTCACAAGGGGCCCTCCCCACAGCAAATCTG
  GAGGTGGGACAGGCGAGGAACCAGGGTCCCAGGGCCTCAATGGGGAGGCAGGACCCTGAG
  ACTCAACTAGGAAACTCCTTCCAAGAAAATGGCCCCACAGCCAAGGCCCTACACAGGCTT
  TTCCTCCAACTCNGAAACGTGGGACTGAGGCANNGCCAAGTGGTGAGCCTGGCATTCCCA
  CTGGTGAGGCTGGGCCTTCCTGCTCTTCAGCCTCTNCAAGCTGCCTCGNAGTGCTAAGTC
  CAGTTCTTTTGAGACAGTGGGGATGAGTCAAATGAGGCGGAGGAAGAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_177983 unedited
  AGCTTGTCCGCGGCCGCATTTTACGTCGAGTTTTTTTTTTTTTTTTTTTTTTAGGCTTTG
  GCCTTTAGTCTGAAAAATGTTGATTGAAAGTGTACAACAGAGAGCGGGTGCAAGCGGCCG
  AGGGCCATGGAGCCGCCAATAAAAAAGAATGTCCTTAAATAAAGTTCACAGAGTAAAAAC
  CAGAACCGCCAGTCCTTCCCTCCAACACAACAGAGCACAGGCACAGAACCGGCGATGAGC
  CCGAGGAGCAGAGGCGGCTGGGAAGGACAGCAGAGGCTCCCGGCTGCAGTGTGGAGGGAG
  AGCCCTCTTTGGAATGGGCGGAGTGAAGCCACCCAGCTCCCCCTGCACACCTCATACCCA
  CTGCTAAGGCTAAAGGAAAAAGACAAAACTCAGTCTCAGGTCCGGAGGGCTCAGAAAACA
  GTCTAGGTGGGCAGGGGTCTGGATGACTGCTAGTCTCGCTTGGCCTTCTTCTTCTTGTCG
  CTGTTGCCATTTTCTTCAGCCCCCTCAGTAGAGAGCACCTCCTCTAGTTTTCGCTTGCCA
  CTCTCTGGCTGGAGCTCTGCTGTGTTTCGGGGCCTTGAAGCACATGATGACGCAGGTCAT
  GTTTGTCCACCCTTGTACATCCCCAGCAGTGTCTGTTGCCAGGGACTGATTCCGCAGCTT
  TTTCCACATGGATGACACTAACCCGAACCTCCCCATTTTCATACCCCTGCTGACCCTTTG
  ATGAAGAAAATTACAATTCCTTGGCTGCTCAACACATTCACAACCCTCACAGGCCCTGAC
  CCTGGAATTCAGGGCCGGCAGTGCAAAGTCACCCCCTTGGTCGCACGAAAAGGCTGAAAA
  CATTTAGTTCCCCCAGGGGCAAGGCTTTTGGTTTTCTTTACCAAAACGGGCCCCCATAGG
  TTCTGGAAAAGGTGGGGCCCCCGGTGATCTCCCCTCCCATGGGGACCCTGCCCCN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_177983
• Insert Size: 2190 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177983.1, NP_817092.1
• RefSeq Size: 2265 bp
• RefSeq ORF: 1641 bp
• Locus ID: 5496
• Cytogenetics: 2p23.3
• Protein Families: Druggable Genome, Phosphatase
• Gene Summary: 'The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase is found to be responsible for the dephosphorylation of Pre-mRNA splicing factors, which is important for the formation of functional spliceosome. Studies of a similar gene in mice suggested a role of this phosphatase in regulating cell cycle progression. [provided by RefSeq, Apr 2010]'