COPA (NM_004371) Human Untagged Clone

CAT#: SC126655

COPA (untagged)-Human coatomer protein complex, subunit alpha (COPA), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: COPA
• Synonyms: AILJK; alpha-COP; HEP-COP
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC126655 sequence for NM_004371 edited (data generated by NextGen Sequencing)
  ATGTTAACCAAATTCGAGACCAAGAGCGCGCGGGTCAAAGGGCTCAGCTTTCACCCCAAA
  AGACCTTGGATCCTGACTAGTTTACATAATGGGGTCATCCAGTTATGGGACTATCGGATG
  TGCACTCTCATTGACAAGTTTGATGAACATGATGGTCCAGTGCGAGGCATTGACTTCCAT
  AAGCAGCAGCCACTGTTCGTCTCTGGAGGAGATGACTATAAGATTAAGGTTTGGAATTAC
  AAGCTTCGGCGCTGTCTTTTCACATTGCTTGGGCACTTAGATTATATTCGCACCACGTTT
  TTTCATCATGAATATCCCTGGATTCTGAGTGCCTCCGATGATCAGACCATCCGAGTGTGG
  AATTGGCAATCTAGAACCTGTGTTTGTGTGTTAACAGGGCACAACCATTATGTGATGTGT
  GCTCAGTTCCACCCCACAGAAGACTTGGTAGTATCAGCCAGCCTGGACCAGACTGTGCGC
  GTTTGGGATATTTCTGGTCTGAGGAAAAAAAACCTGTCCCCTGGTGCGGTGGAATCGGAT
  GTGAGAGGAATAACTGGGGTTGATCTATTTGGAACTACAGATGCAGTGGTGAAGCATGTA
  CTAGAGGGTCACGATCGTGGAGTAAACTGGGCTGCCTTCCACCCCACTATGCCCCTTATT
  GTATCTGGGGCAGATGATCGTCAAGTGAAGATCTGGCGCATGAATGAATCAAAGGCATGG
  GAGGTTGATACCTGCCGGGGCCATTACAACAATGTATCTTGTGCCGTCTTCCACCCTCGC
  CAAGAGTTGATCCTCAGCAATTCTGAGGACAAGAGTATTCGAGTCTGGGATATGTCTAAG
  CGGACTGGGGTTCAGACTTTCCGCAGAGACCATGATCGTTTCTGGGTCCTAGCTGCTCAC
  CCTAACCTTAACCTCTTTGCAGCAGGCCATGATGGTGGTATGATTGTGTTTAAGCTGGAA
  CGGGAACGGCCAGCCTATGCTGTTCATGGCAATATGCTACACTATGTCAAGGACCGATTC
  TTACGACAGCTGGATTTCAACAGCTCCAAAGATGTAGCTGTGATGCAGTTGCGGAGTGGT
  TCCAAGTTTCCAGTATTCAATATGTCATACAATCCAGCAGAAAATGCAGTCCTGCTTTGT
  ACAAGAGCTAGCAATCTAGAGAATAGTACCTATGACCTGTACACCATCCCTAAAGATGCT
  GACTCCCAGAATCCTGATGCGCCTGAAGGGAAACGATCCTCAGGCCTGACAGCCGTTTGG
  GTCGCTCGAAATCGGTTTGCTGTCCTAGATCGGATGCATTCGCTTCTGATCAAGAATCTG
  AAGAATGAGATCACCAAAAAGGTACAGGTGCCCAACTGTGATGAGATCTTCTATGCTGGC
  ACAGGCAATCTCCTGCTTCGAGATGCGGACTCTATCACACTCTTTGACGTACAGCAGAAG
  CGGACTCTGGCATCTGTGAAGATTTCTAAAGTGAAATACGTTATCTGGTCAGCAGACATG
  TCACATGTAGCACTACTAGCCAAACACGCCATTGTGATCTGTAACCGCAAACTGGATGCT
  TTATGTAACATTCATGAGAACATTCGTGTCAAGAGTGGGGCCTGGGATGAGAGTGGGGTA
  TTTATCTATACCACAAGCAACCACATCAAATATGCTGTCACCACTGGGGACCACGGGATC
  ATTCGAACTCTGGATTTACCCATCTATGTCACACGGGTGAAGGGCAACAATGTATACTGC
  CTAGACAGGGAGTGTCGTCCCCGGGTACTCACCATTGATCCCACTGAGTTCAAATTCAAG
  CTGGCCCTGATCAACAGAAAATATGATGAGGTACTGCACATGGTGAGGAATGCCAAACTA
  GTTGGCCAGTCTATTATTGCTTATCTCCAGAAGAAGGGCTATCCTGAAGTGGCACTGCAT
  TTTGTCAAGGATGAGAAAACTCGCTTTAGTCTGGCACTGGAGTGTGGAAACATTGAGATT
  GCTCTGGAAGCAGCCAAAGCACTGGATGACAAGAACTGCTGGGAAAAGCTGGGAGAAGTG
  GCCCTGCTGCAGGGGAACCACCAGATTGTGGAAATGTGCTATCAGCGTACCAAAAACTTT
  GACAAACTTTCCTTCCTGTATCTTATCACTGGCAACTTAGAAAAACTTCGCAAGATGATG
  AAGATTGCTGAGATCAGAAAGGACATGAGTGGCCACTATCAGAATGCCCTATACCTGGGT
  GATGTGTCAGAGCGTGTGCGGATCCTGAAGAACTGTGGACAGAAGTCCCTGGCCTATCTC
  ACAGCTGCTACCCATGGCTTAGATGAAGAAGCTGAGAGCCTAAAGGAGACATTTGACCCA
  GAGAAGGAGACAATCCCAGACATTGACCCTAATGCCAAGCTGCTCCAGCCACCTGCACCT
  ATCATGCCATTGGATACCAATTGGCCTTTATTGACTGTATCCAAAGGATTTTTTGAAGGC
  ACCATTGCCAGCAAAGGGAAGGGAGGAGCACTGGCTGCTGACATTGACATTGACACTGTT
  GGTACAGAGGGCTGGGGAGAGGATGCAGAGCTGCAGTTGGATGAAGATGGGTTTGTGGAG
  GCTACAGAAGGTTTGGGGGATGATGCTCTTGGCAAGGGACAGGAAGAAGGAGGTGGCTGG
  GATGTAGAAGAAGATCTGGAGCTCCCTCCTGAGCTGGATATATCCCCTGGGGCAGCTGGT
  GGGGCTGAAGATGGTTTCTTTGTGCCCCCAACCAAGGGAACAAGTCCAACTCAGATCTGG
  TGTAATAACTCTCAGCTTCCAGTTGATCACATCCTGGCAGGCTCTTTCGAAACAGCCATG
  CGGCTCCTTCATGACCAAGTAGGGGTAATCCAGTTTGGCCCCTACAAGCAACTGTTCCTA
  CAGACATACGCCCGAGGCCGCACAACCTATCAGGCTCTGCCCTGCCTACCCTCCATGTAT
  GGCTATCCTAATCGCAACTGGAAGGATGCAGGGCTGAAGAATGGTGTACCAGCTGTGGGC
  CTGAAGCTTAATGACCTCATCCAACGGTTGCAGCTGTGCTACCAGCTCACCACAGTTGGC
  AAATTTGAGGAGGCTGTGGAAAAATTCCGTTCCATCCTTCTCAGTGTGCCACTTCTTGTT
  GTGGACAATAAACAAGAGATTGCAGAGGCCCAGCAGCTCATCACCATTTGCCGTGAGTAC
  ATTGTGGGTTTGTCCGTGGAGACAGAAAGGAAGAAGCTGCCCAAAGAGACTCTAGAACAG
  CAGAAGCGCATCTGTGAGATGGCAGCCTATTTCACCCACTCAAACCTGCAGCCTGTGCAC
  ATGATCCTGGTGCTGCGTACAGCCCTCAATCTGTTCTTCAAGCTCAAGAACTTCAAGACA
  GCTGCCACCTTTGCTCGGCGCCTACTAGAACTCGGGCCCAAGCCTGAGGTGGCCCAACAG
  ACCCGAAAAATCCTGTCTGCCTGTGAGAAGAATCCCACAGATGCCTACCAGCTCAATTAT
  GACATGCACAACCCCTTTGACATTTGTGCTGCATCATATCGGCCCATCTACCGTGGAAAG
  CCAGTAGAAAAGTGTCCACTCAGTGGGGCCTGCTATTCCCCTGAGTTCAAAGGTCAAATC
  TGCAGGGTCACCACAGTGACAGAGATTGGCAAAGATGTGATTGGTTTAAGGATCAGTCCT
  CTGCAGTTTCGCTAA
  
  Clone variation with respect to NM_004371.3
  363 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004371 unedited
  GCCGCGAATTCGGCACGAGCTGACGTGGAGGCGTCCGAAGGGCAGCAGGGTGTGTCGGGG
  CTCGGATTAAGACATCGGAGTCGGAGACCTGAGAGATGTTAACCAAATTCGAGACCAAGA
  GCGCGCGGGTCAAAGGGCTCAGCTTTCACCCCAAAAGACCTTGGATCCTGACTAGTTTAC
  ATAATGGGGTCATCCAGTTATGGGACTATCGGATGTGCACTCTCATTGACAAGTTTGATG
  AACATGATGGTCCAGTGCGAGGCATTGACTTCCATAAGCAGCAGCCACTGTTCGTCTCTG
  GAGGAGATGACTATAAGATTAAGGTTTGGAATTACAAGCTTCGGCGCTGTCTTTTCACAT
  TGCTTGGGCACTTAGATTATATTCGCACCACGTTTTTTCATCATGAATATCCCTGGATTC
  TGAGTGCCTCCGATGATCAGACCATCCGAGTGTGGAATTGGCAATCTAGAACCTGTGTTT
  GTGTGTTAACAGGGCACAACCATTATGTGATGTGTGCTCAGTTCCACCCCACAGAAGACT
  TGGTAGTATCAGCCAGCCTGGACCAGACTGTGCGCGTTTGGGATATTTCTGGTCTGAGGA
  AAAAAAACCTGTCCCCTGGTGCGGTGGAATCGGATGTGAGAGGAATAACTGGGGTTGATC
  TATNTGGAACTACAGATGCAGTGGTGAAGCATGTACTAGAGGGTCACGATCGTGGAGTAA
  ACTGGGN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004371
• Insert Size: 4310 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004371.2, NP_004362.1
• RefSeq Size: 5064 bp
• RefSeq ORF: 3675 bp
• Locus ID: 1314
• Cytogenetics: 1q23.2
• Domains: WD40, Coatomer_WDAD
• Gene Summary: 'In eukaryotic cells, protein transport between the endoplasmic reticulum and Golgi compartments is mediated in part by non-clathrin-coated vesicular coat proteins (COPs). Seven coat proteins have been identified, and they represent subunits of a complex known as coatomer. The subunits are designated alpha-COP, beta-COP, beta-prime-COP, gamma-COP, delta-COP, epsilon-COP, and zeta-COP. The alpha-COP, encoded by COPA, shares high sequence similarity with RET1P, the alpha subunit of the coatomer complex in yeast. Also, the N-terminal 25 amino acids of alpha-COP encode the bioactive peptide, xenin, which stimulates exocrine pancreatic secretion and may act as a gastrointestinal hormone. Alternative splicing results in multiple splice forms encoding distinct isoforms. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1, resulting in a shorter protein (isoform 2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.