TAK1 (MAP3K7) (NM_145333) Human Untagged Clone

CAT#: SC126713

MAP3K7 (untagged)-Human mitogen-activated protein kinase kinase kinase 7 (MAP3K7), transcript variant D

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MAP3K7
• Synonyms: CSCF; FMD2; MEKK7; TAK1; TGF1a
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC126713 sequence for NM_145333 edited (data generated by NextGen Sequencing)
  ATGTCTACAGCCTCTGCCGCCTCCTCCTCCTCCTCGTCTTCGGCCGGTGAGATGATCGAA
  GCCCCTTCCCAGGTCCTCAACTTTGAAGAGATCGACTACAAGGAGATCGAGGTGGAAGAG
  GTTGTTGGAAGAGGAGCCTTTGGAGTTGTTTGCAAAGCTAAGTGGAGAGCAAAAGATGTT
  GCTATTAAACAAATAGAAAGTGAATCTGAGAGGAAAGCGTTTATTGTAGAGCTTCGGCAG
  TTATCCCGTGTGAACCATCCTAATATTGTAAAGCTTTATGGAGCCTGCTTGAATCCAGTG
  TGTCTTGTGATGGAATATGCTGAAGGGGGCTCTTTATATAATGTGCTGCATGGTGCTGAA
  CCATTGCCATATTATACTGCTGCCCACGCAATGAGTTGGTGTTTACAGTGTTCCCAAGGA
  GTGGCTTATCTTCACAGCATGCAACCCAAAGCGCTAATTCACAGGGACCTGAAACCACCA
  AACTTACTGCTGGTTGCAGGGGGGACAGTTCTAAAAATTTGTGATTTTGGTACAGCCTGT
  GACATTCAGACACACATGACCAATAACAAGGGGAGTGCTGCTTGGATGGCACCTGAAGTT
  TTTGAAGGTAGTAATTACAGTGAAAAATGTGACGTCTTCAGCTGGGGTATTATTCTTTGG
  GAAGTGATAACGCGTCGGAAACCCTTTGATGAGATTGGTGGCCCAGCTTTCCGAATCATG
  TGGGCTGTTCATAATGGTACTCGACCACCACTGATAAAAAATTTACCTAAGCCCATTGAG
  AGCCTGATGACTCGTTGTTGGTCTAAAGATCCTTCCCAGCGCCCTTCAATGGAGGAAATT
  GTGAAAATAATGACTCACTTGATGCGGTACTTTCCAGGAGCAGATGAGCCATTACAGTAT
  CCTTGTCAGTATTCAGATGAAGGACAGAGCAACTCTGCCACCAGTACAGGCTCATTCATG
  GACATTGCTTCTACAAATACGAGTAACAAAAGTGACACTAATATGGAGCAAGTTCCTGCC
  ACAAATGATACTATTAAGCGCTTAGAATCAAAATTGTTGAAAAATCAGGCAAAGCAACAG
  AGTGAATCTGGACGTTTAAGCTTGGGAGCCTCCCGTGGGAGCAGTGTGGAGAGCTTGCCC
  CCAACCTCTGAGGGCAAGAGGATGAGTGCTGACATGTCTGAAATAGAAGCTAGGATCGCC
  GCAACCACAGGCAACGGACAGCCAAGACGTAGATCCATCCAAGACTTGACTGTAACTGGA
  ACAGAACCTGGTCAGGTGAGCAGTAGGTCATCCAGTCCCAGTGTCAGAATGATTACTACC
  TCAGGACCAACCTCAGAAAAGCCAACTCGAAGTCATCCATGGACCCCTGATGATTCCACA
  GATACCAATGGATCAGATAACTCCATCCCAATGGCTTATCTTACACTGGATCACCAACTA
  CAGGCAAGAACTAGTTGCAGAACTGGACCAGGATGA
  
  Clone variation with respect to NM_145333.1
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_145333 unedited
  CGCGCGCGAATTCGGCACGAGCGGACACGGCTGTGGCCGCTGCCTCTACCCCCGCCACGG
  ATCGCCGGGTAGTAGGACTGCGCGGCTCCAGGCTGAGGGTCGGTCCGGAGGCGGGTGGGC
  GCGGGTCTCACCCGGATTGTCCGGGTGGCACCGTTCCCGGCCCCACCGGGCGCCGCGAGG
  GATCATGTCTACAGCCTCTGCCGCCTCCTCCTCCTCCTCGTCTTCGGCCGGTGAGATGAT
  CGAAGCCCCTTCCCAGGTCCTCAACTTTGAAGAGATCGACTACAAGGAGATCGAGGTGGA
  AGAGGTTGTTGGAAGAGGAGCCTTTGGAGTTGTTTGCAAAGCTAAGTGGAGAGCAAAAGA
  TGTTGCTATTAAACAAATAGAAAGTGAATCTGAGAGGAAAGCGTTTATTGTAGAGCTTTG
  GCAGTTATCCCGGGTGAACCATCCTAATATTGTACAGCCCTTGCAGCCTGTTACCCCCCC
  CGCGCCCTGTGATCGGATACGCCGACCGCGCGCTCTTTAATAATGCGCTGAATGAAGCTC
  CCCTTCTCCTTATTCACCGACCTTCACTTTTGTCCTCGCCTCCATTATCGTTCCTAACTC
  TGTCTCCACCCCCCCCCTCCCTCCCAACCACCAACTACAACCGCCCCCCTCCCCGCCTCC
  CTTTTTTCCCTTCTCCGCCCCTCCCCCATCACTCCTACTATTTTCATCCTCTTATCACCC
  CCCCTCTTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_145333
• Insert Size: 1920 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145333.1, NP_663306.1
• RefSeq Size: 2796 bp
• RefSeq ORF: 1476 bp
• Locus ID: 6885
• Cytogenetics: 6q15
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Adherens junction, MAPK signaling pathway, NOD-like receptor signaling pathway, RIG-I-like receptor signaling pathway, T cell receptor signaling pathway, Toll-like receptor signaling pathway, Wnt signaling pathway
• Gene Summary: 'The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase mediates the signaling transduction induced by TGF beta and morphogenetic protein (BMP), and controls a variety of cell functions including transcription regulation and apoptosis. In response to IL-1, this protein forms a kinase complex including TRAF6, MAP3K7P1/TAB1 and MAP3K7P2/TAB2; this complex is required for the activation of nuclear factor kappa B. This kinase can also activate MAPK8/JNK, MAP2K4/MKK4, and thus plays a role in the cell response to environmental stresses. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (D) lacks two segments, one of which leads to a frameshift, compared to variant C. The resulting isoform (C) lacks an internal region, and contains a distinct and shorter C-terminus, as compared to isoform B. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.