POLR2G (NM_002696) Human Untagged Clone

CAT#: SC126714

POLR2G (untagged)-Human polymerase (RNA) II (DNA directed) polypeptide G (POLR2G)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: POLR2G
• Synonyms: hRPB19; hsRPB7; RPB7; RPB19
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC126714 sequence for NM_002696 edited (data generated by NextGen Sequencing)
  ATGTTCTACCATATCTCCCTAGAGCACGAAATCCTGCTGCACCCGCGCTACTTCGGCCCC
  AACTTGCTCAACACGGTGAAGCAGAAGCTCTTCACCGAGGTGGAGGGGACCTGCACAGGG
  AAGTATGGCTTTGTAATTGCTGTCACCACCATTGACAATATTGGTGCTGGTGTGATCCAG
  CCAGGCCGAGGCTTTGTCCTTTATCCAGTTAAGTACAAGGCCATTGTTTTCCGGCCATTT
  AAAGGGGAGGTCGTGGATGCTGTTGTCACTCAGGTCAACAAGGTTGGACTCTTCACAGAA
  ATTGGGCCCATGTCTTGCTTCATCTCTCGACATTCCATCCCTTCAGAGATGGAGTTTGAT
  CCTAACTCCAACCCACCATGTTACAAGACAATGGATGAGGATATTGTGATTCAGCAGGAC
  GATGAGATCCGCTTAAAGATTGTGGGGACCCGTGTGGACAAGAATGACATTTTTGCTATT
  GGCTCCCTGATGGACGATTACTTGGGGCTTGTAAGCTGA
  
  Clone variation with respect to NM_002696.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002696 unedited
  TCAGATTTTGTAATACGACTCACTATAGGGCGGCACGCGAATTCGCACCAAAGGACTGTC
  GGAGGTGTGGACTCTGCCTGCCTACCTGGTCTGGGAAGATGTTCTACCATATCTCCCTAT
  AGCACGAAATCCTGCTGCACCCGCGCTACTTCGGCCCCAACTTGCTCAACACGGTGAAGC
  AGAAGCTCTTCACCGAGGTGGAGGGGACCTGCACAGGGAAGTATGGCTTTGTAATTGCTG
  TCACCACCATTGACAATATTGGTGCTGGTGTGATCCAGCCAGGCCGAGGCTTTGTCCTTT
  ATCCAGTTAAGTACAAGGCCATTGTTTTCCGGCCATTTAAAGGGGAAGTCGTGGATGCTG
  TTGTCACTCAAGTCAACAAAGTTGGACTCTTCACAGAAATTGGGCCCATGTCTTGCTTCA
  TCTCTCGACATTCCATCCCTTCAGAGATGGAGTTTGATCCTAACTCCAACCCACCATGTT
  ACAAGACAATGGATGATGATATTGTGATTCAGCAGGACGATGATATCCGCTTAAAGATTG
  TGGGGACCCGTGTGGACAAGAATGACATTTTTGCTATTGGCTCCCTGATGGACGATTACT
  TGGGGCTTGTAAGCTGAGCCTGGTGGCCTCCTACCCTTGGTCCTACTCTATGAAGTGTGA
  TTGTCACACTTATCATGTTGTCCATATGTCCAGTCTGGCTGCTGTTGTGGATGCAATGAA
  TGCATCTCATCCCATAATGCATCTGGTGCTTCTTGTAGCTAACTACTGNCTNCTCATTNT
  TCAGTATGAGTTCTAGTATAAAAAGNCCTGGNCTCTCAAAAAAAAAAAAAAAAAAAACCG
  ACTCTAGATGCGGGCGCGTCATAGCTGTTTCTGACAGATCCAGGTAGCATCCTGTGACCC
  TCCCATGCCTCTCTGCCCTGAATTGCACTCATGCCACATCCTGTCTATAAATTATT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002696
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002696.1, NP_002687.1
• RefSeq Size: 828 bp
• RefSeq ORF: 519 bp
• Locus ID: 5436
• Cytogenetics: 11q12.3
• Domains: S1, RNA_pol_Rpb7_N
• Protein Families: Transcription Factors
• Protein Pathways: Huntington's disease, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase
• Gene Summary: 'This gene encodes the seventh largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The protein functions in transcription initiation, and is also thought to help stabilize transcribing polyermase molecules during elongation. [provided by RefSeq, Jan 2009]'