SNF5 (SMARCB1) (NM_003073) Human Untagged Clone

CAT#: SC126727

SMARCB1 (untagged)-Human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1 (SMARCB1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SMARCB1
• Synonyms: BAF47; CSS3; hSNFS; INI1; MRD15; PPP1R144; RDT; RTPS1; Sfh1p; SNF5; SNF5L1; Snr1; SWNTS1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC126727 sequence for NM_003073 edited (data generated by NextGen Sequencing)
  ATGATGATGATGGCGCTGAGCAAGACCTTCGGGCAGAAGCCCGTGAAGTTCCAGCTGGAG
  GACGACGGCGAGTTCTACATGATCGGCTCCGAGGTGGGAAACTACCTCCGTATGTTCCGA
  GGTTCTCTGTACAAGAGATACCCCTCACTCTGGAGGCGACTAGCCACTGTGGAAGAGAGG
  AAGAAAATAGTTGCATCGTCACATGGTAAAAAAACAAAACCTAACACTAAGGATCACGGA
  TACACGACTCTAGCCACCAGTGTGACCCTGTTAAAAGCCTCGGAAGTGGAAGAGATTCTG
  GATGGCAACGATGAGAAGTACAAGGCTGTGTCCATCAGCACAGAGCCCCCCACCTACCTC
  AGGGAACAGAAGGCCAAGAGGAACAGCCAGTGGGTACCCACCCTGCCCAACAGCTCCCAC
  CACTTAGATGCCGTGCCATGCTCCACAACCATCAACAGGAACCGCATGGGCCGAGACAAG
  AAGAGAACCTTCCCCCTTTGCTTTGATGACCATGACCCAGCTGTGATCCATGAGAACGCA
  TCTCAGCCCGAGGTGCTGGTCCCCATCCGGCTGGACATGGAGATCGATGGGCAGAAGCTG
  CGAGACGCCTTCACCTGGAACATGAATGAGAAGTTGATGACGCCTGAGATGTTTTCAGAA
  ATCCTCTGTGACGATCTGGATTTGAACCCGCTGACGTTTGTGCCAGCCATCGCCTCTGCC
  ATCAGACAGCAGATCGAGTCCTACCCCACGGACAGCATCCTGGAGGACCAGTCAGACCAG
  CGCGTCATCATCAAGCTGAACATCCATGTGGGAAACATTTCCCTGGTGGACCAGTTTGAG
  TGGGACATGTCAGAGAAGGAGAACTCACCAGAGAAGTTTGCCCTGAAGCTGTGCTCGGAG
  CTGGGGTTGGGCGGGGAGTTTGTCACCACCATCGCATACAGCATCCGGGGACAGCTGAGC
  TGGCATCAGAAGACCTACGCCTTCAGCGAGAACCCTCTGCCCACAGTGGAGATTGCCATC
  CGGAACACGGGCGATGCGGACCAGTGGTGCCCACTGCTGGAGACTCTGACAGACGCTGAG
  ATGGAGAAGAAGATCCGCGACCAGGACAGGAACACGAGGCGGATGAGGCGTCTTGCCAAC
  ACGGCCCCGGCCTGGTAA
  
  Clone variation with respect to NM_003073.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003073 unedited
  AATTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTGAGGCGCCAG
  TACCCGGCCCGGTCCGCATTTCGCCTTCCGGCTTCGGTTTCCCTCGGCCCAGCACGCCCC
  GGCCCCGCCCCAGCCCTCCTGATCCCTCGCAGCCCGGCTCCGGCCGCCCGCCTCTGCCGC
  CGCAATGATGATGATGGCGCTGAGCAAGACCTTCGGGCAGAAGCCCGTGAAGTTCCAGCT
  GGAGGACGACGGCGAGTTCTACATGATCGGCTCCGAGGTGGGAAACTACCTCCGTATGTT
  CCGAGGTTCTCTGTACAAGAGATACCCCTCACTCTGGAGGCGACTAGCCACTGTGGAAGA
  GAGGAAGAAAATAGTTGCATCGTCACATGGTAAAAAAACAAAACCTAACACTAAGGATCA
  CGGATACACGACTCTAGCCACCAGTGTGACCCTGTTAAAAGCCTCGGAAGTGGAAGAGAT
  TCTGGATGGCAACGATGAGAAGTACAAGGCTGTGTCCATCAGCACAGAGCCCCCCACCTA
  CCTCAGGGAACAGAAGGCCNNNAGGAACAGCCAGTGGGTACCCACCCTGCCCAACAGCTC
  CCACCACTTAGATGCCGTGCCATGCTCCACAACCATCAACAGGAACCGCATGGGCCGAGA
  CAAGAGAGAACCTTCCCCCTTTGCTNTGATGACCATGACCCAGCTGTGATCCATGAGAAC
  GCATCTCAGCCCCGAGTGCTGGTCCCCCATCCGCTGGACATGGAGATCGATGGGCAGAAG
  CTGCGAGACGCCTTCACCTGGACATGAATGAGAAGTTGATGACGCCTGAGATGTTTTCAG
  AATCCTCTGTGACGATCTGGATTGAACCCGCTGACGTTTGTGCN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003073 unedited
  GCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTGTTGAAGAAAATTGAACATGA
  CCTGTTGCCTTTTATTTAAAAACTGTTACTAGCCCTGCCTGGGGCTCCTATACAAAAACA
  AAACACAACCTAAAATAAGGTTTCTTCCTGACCCCAAAAACTGGGGAGGGGTAGGGAGGG
  TGGGNGTCCCCCCCCCCCCCTCCCCCCCCCCCCCCCCCTTCCCTCCCCCCCCCCCCCCCT
  CCCCCTCCCCCCCCCTTCCCCCTTCTTCCCCCTCTCTTCCCCTTCCCTTCCCCCCCCCCC
  CCCCCCCCTCTCTTCCCCTCCTCCCCCCCCCCTCCCCCTTCCCTCCTTTCCTCCCCTCCT
  CCCCCTCTCCCCTCCCCTCCTCTCCCCCCCCCTCCCCCCCCTCCCCCCCTTTCCTCCCCT
  TCCCCCCCCCCCCCCCCCCCCCTCCCCCCCCCCCTTCTCCCCCCCTCCTCCTCTTCCTCC
  CCTCCCTCCCCCTCTCCCCCCCCCCTTCCTCTCCCCCTCCCCCTCCCCCCTCCCCCCCTT
  CCCCTCCGCCCTTCCCCCCCTCCCCTTTTTCCCCCCTTCCCCCCCCCCCCTTCCCCCCCC
  TCCCCCCTCCCCCCGTTCCCTACCCCATTCCCCCCCCCCCCCCCCCGCTTCCCTTTCCCC
  CTCCTCCCCCTCCCTTCCCTCTTCCCCCCCCTCCGGTCTTCCCCCCCCTCCATCCCTTCC
  CCTTTCCCCCCCCCGCCCCCCTCCGTCCTCCCCTCCTCCTCCTCCCCCTCCCCTCCCTTC
  CCTTCTCCTCTCCCCCCTCCTCTTTTCCCCCCCTCTTCCCCCCGTCCTTCCCCCCGTCCT
  TCCCCCCGTCCCCCCTTCCCGTCCCCTCCCCCCCCCGTCTTCCCCTCTCCCCCCTTTCCC
  CCCCTTTCTCCCCCCCCCTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003073
• Insert Size: 1710 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003073.3, NP_003064.2
• RefSeq Size: 1717 bp
• RefSeq ORF: 1158 bp
• Locus ID: 6598
• Cytogenetics: 22q11
• Domains: SNF5
• Protein Families: Transcription Factors
• Gene Summary: 'The protein encoded by this gene is part of a complex that relieves repressive chromatin structures, allowing the transcriptional machinery to access its targets more effectively. The encoded nuclear protein may also bind to and enhance the DNA joining activity of HIV-1 integrase. This gene has been found to be a tumor suppressor, and mutations in it have been associated with malignant rhabdoid tumors. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2015]'
  Transcript Variant: This variant (1) encodes isoform a.