SF3B3 (NM_012426) Human Untagged Clone

CAT#: SC126731

SF3B3 (untagged)-Human splicing factor 3b, subunit 3, 130kDa (SF3B3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SF3B3
• Synonyms: RSE1; SAP130; SF3b130; STAF130
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC126731 sequence for NM_012426 edited (data generated by NextGen Sequencing)
  ATGTTTCTGTACAACTTAACCTTGCAGAGAGCCACTGGCATCAGCTTTGCCATTCATGGA
  AACTTTTCTGGAACCAAACAACAAGAAATTGTTGTTTCCCGTGGGAAGATCTTGGAGCTG
  CTTCGCCCAGACCCCAACACTGGCAAAGTACATACCCTACTCACTGTGGAAGTATTCGGT
  GTTATCCGGTCACTCATGGCCTTTAGGCTGACAGGTGGCACCAAAGACTACATTGTAGTT
  GGCAGTGACTCTGGTCGAATTGTTATTTTGGAATACCAGCCATCTAAGAATATGTTTGAG
  AAGATTCACCAAGAAACCTTTGGCAAGAGTGGATGCCGTCGCATCGTTCCTGGCCAGTTC
  TTAGCTGTGGATCCCAAAGGGCGAGCCGTTATGATTAGTGCCATTGAGAAACAGAAATTG
  GTGTATATTTTGAACAGAGATGCTGCAGCCCGACTTACCATTTCATCTCCCCTGGAAGCC
  CACAAAGCAAACACTTTAGTGTATCATGTAGTTGGAGTAGATGTCGGATTTGAAAATCCA
  ATGTTTGCTTGTCTGGAAATGGATTATGAGGAAGCAGACAATGATCCAACAGGGGAAGCA
  GCAGCTAATACCCAGCAGACACTTACTTTCTATGAGCTAGACCTTGGTTTAAATCATGTG
  GTCCGAAAATACAGTGAACCTTTGGAGGAACACGGCAACTTCCTTATTACAGTTCCTGGA
  GGGTCAGATGGTCCAAGTGGAGTACTGATCTGCTCTGAAAACTATATTACTTACAAGAAC
  TTTGGTGACCAGCCAGATATCCGCTGTCCAATTCCCAGGAGGCGGAATGACCTGGATGAC
  CCTGAAAGAGGAATGATTTTTGTCTGCTCTGCAACCCATAAAACCAAATCGATGTTCTTC
  TTTTTGGCTCAAACTGAGCAGGGAGATATCTTTAAGATCACTTTGGAGACAGATGAAGAT
  ATGGTTACTGAGATCCGGCTCAAATATTTTGATACTGTACCCGTTGCTGCTGCCATGTGT
  GTGCTTAAAACAGGGTTCCTTTTTGTAGCATCAGAATTTGGAAACCATTACTTATATCAA
  ATTGCACATCTTGGAGATGATGATGAAGAACCTGAGTTTTCATCAGCCATGCCTCTGGAA
  GAAGGAGACACATTCTTTTTTCAGCCAAGACCACTTAAAAACCTTGTGCTGGTTGATGAG
  TTGGACAGCCTCTCTCCCATTCTGTTTTGCCAGATAGCTGATCTGGCCAATGAAGATACT
  CCACAGTTGTATGTGGCCTGTGGTAGGGGACCCCGATCATCTCTGAGAGTCCTAAGACAT
  GGACTTGAGGTGTCAGAAATGGCTGTTTCTGAGCTACCTGGTAACCCCAACGCTGTCTGG
  ACAGTGCGTCGACACATTGAAGATGAGTTTGATGCCTACATCATTGTGTCTTTCGTGAAT
  GCCACCCTAGTGTTGTCCATTGGAGAAACTGTAGAAGAAGTGACTGACTCTGGGTTCCTG
  GGGACCACCCCGACCTTGTCCTGCTCCTTATTAGGAGATGATGCCTTGGTGCAGGTCTAT
  CCAGATGGCATTCGGCACATACGAGCAGACAAGAGAGTCAATGAGTGGAAGACCCCTGGA
  AAGAAAACAATTGTGAAGTGTGCAGTGAACCAGCGACAAGTGGTGATTGCCCTGACAGGA
  GGAGAGCTGGTCTATTTCGAGATGGATCCTTCAGGACAGCTGAATGAGTACACAGAACGG
  AAGGAGATGTCAGCAGATGTGGTGTGCATGAGTCTGGCCAATGTACCCCCTGGAGAGCAG
  CGGTCTCGCTTCCTGGCTGTGGGGCTTGTGGACAACACTGTCAGAATCATCTCCCTGGAT
  CCCTCAGACTGTTTGCAACCTCTAAGCATGCAGGCTCTCCCAGCCCAGCCTGAGTCCTTG
  TGTATCGTGGAAATGGGTGGGACTGAGAAGCAGGATGAGCTGGGTGAGAGGGGCTCGATT
  GGCTTCCTATACCTGAATATTGGGCTACAGAACGGTGTGCTGCTGAGGACTGTCTTGGAC
  CCTGTCACTGGGGATTTGTCTGATACTCGCACTCGGTACCTGGGGTCCCGTCCTGTGAAG
  CTCTTCCGAGTCCGAATGCAAGGCCAGGAGGCAGTATTGGCCATGTCAAGCCGCTCATGG
  TTGAGCTATTCTTACCAATCTCGCTTCCATCTCACCCCACTGTCTTACGAGACACTGGAA
  TTTGCATCGGGTTTTGCCTCGGAACAGTGTCCCGAGGGCATTGTGGCCATCTCCACCAAC
  ACCCTACGGATTTTGGCATTAGAGAAGCTCGGTGCTGTCTTCAATCAAGTAGCCTTCCCA
  CTGCAGTACACACCCAGGAAATTTGTCATCCACCCTGAGAGTAACAACCTTATTATCATT
  GAAACGGACCACAATGCCTACACTGAGGCCACGAAAGCTCAGAGAAAGCAGCAGATGGCA
  GAGGAAATGGTGGAAGCAGCAGGGGAGGATGAGCGGGAGCTGGCCGCAGAGATGGCAGCA
  GCATTCCTCAATGAAAACCTCCCTGAATCCATCTTTGGAGCTCCCAAGGCTGGCAATGGG
  CAGTGGGCCTCTGTGATCCGAGTGATGAATCCCATTCAAGGGAACACACTGGACCTTGTC
  CAGCTGGAACAGAATGAGGCAGCTTTTAGTGTGGCTGTGTGCAGGTTTTCCAACACTGGT
  GAAGACTGGTATGTGCTGGTGGGTGTGGCCAAGGACCTGATACTAAACCCCCGATCTGTG
  GCAGGGGGCTTCGTCTATACTTACAAGCTTGTGAACAATGGGGAAAAACTGGAGTTTTTG
  CACAAGACTCCTGTGGAAGAGGTCCCTGCTGCTATTGCCCCATTCCAGGGGAGGGTGTTG
  ATTGGTGTGGGGAAGCTGTTGCGTGTCTATGACCTGGGAAAGAAGAAGTTACTCCGAAAA
  TGTGAGAATAAGCATATTGCCAATTATATCTCTGGGATCCAGACTATTGGACATAGGGTA
  ATTGTATCTGATGTCCAAGAAAGTTTCATCTGGGTTCGCTACAAGCGTAATGAAAACCAG
  CTTATCATCTTTGCTGATGATACCTACCCCCGATGGGTCACTACAGCCAGCCTCCTGGAC
  TATGACACTGTGGCTGGGGCAGACAAGTTTGGCAACATATGTGTGGTGAGGCTCCCACCT
  AACACCAATGATGAAGTAGATGAGGATCCTACAGGAAACAAAGCCCTGTGGGACCGTGGC
  TTGCTCAATGGGGCCTCCCAGAAGGCAGAGGTGATCATGAACTACCATGTCGGGGAGACG
  GTGCTGTCCTTGCAGAAGACCACGCTGATCCCTGGAGGCTCAGAATCACTTGTCTATACC
  ACCTTGTCTGGAGGAATTGGCATCCTTGTGCCATTCACGTCCCATGAGGACCATGACTTC
  TTCCAGCATGTGGAAATGCACCTGCGGTCTGAACATCCCCCTCTCTGTGGGCGGGACCAC
  CTCAGCTTTCGCTCCTACTACTTCCCTGTGAAGAATGTGATTGATGGAGACCTCTGTGAG
  CAGTTCAATTCCATGGAACCCAACAAACAAAAGAACGTCTCTGAAGAACTGGACCGAACC
  CCACCCGAAGTGTCCAAGAAACTCGAGGATATCCGGACCCGCTACGCCTTCTGA
  
  Clone variation with respect to NM_012426.4
  717 a=>t;2988 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_012426 unedited
  GCGAATTCGGCACGAGGTCTAATGGCGGACGCCAGTATGTTGGAGTTGGTGGTGGCTTAA
  GTTTTGAAGGGAGGTAGCATCCGTTGGATATCCACACCATCCTTCTCGCTGCAGGCTTTC
  TTGGACTCCGTACTGTTGGTGTAACCAAGGCCTGGAGGTCTGGGTGGCTCAGGTTTCCTG
  CAGCCATGTTTCTGTACAACTTAACCTTGCAGAGAGCCACTGGCATCAGCTTTGCCATTC
  ATGGAAACTTTTCTGGAACCAAACAACAAGAAATTGTTGTTTCCCGTGGGAAGATCTTGG
  AGCTGCTTCGCCCAGACCCCAACACTGGCAAAGTACATACCCTACTCACTGTGGAAGTAT
  TCGGTGTTATCCGGTCACTCATGGCCTTTAGGCTGACAGGTGGCACCAAAGACTACATTG
  TAGTTGGCAGTGACTCTGGTCGAATTGTTATTTTGGAATACCAGCCATCTAAGAATATGT
  TTGAGAAGATTCACCAAGAAACCTTTGGCAAGAGTGGATGCCGTCGCATCGTTCCTGGCC
  AGTTCTTAGCTGTGGATCCCAAAGGGCGAGCCGTTATGATTAGTGCCATTGAGAAACAGA
  AATTGGTGTATATTTTGAACAGAGATGCTGCAGCCCGACTTACCATTTCATCTCCCCTGG
  AAGCCCACAAAGCANACACTTTAGTGTATCATGTAGTTGGAGTAGATGTCGGATTTGAAA
  TCCAATGTTTGCTTGTCTGGA
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_012426
• ORF Size: 3654 bp
• Insert Size: 4430
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_012426.3, NP_036558.3
• RefSeq Size: 4357
• RefSeq ORF: 3654
• Locus ID: 23450
• Domains: CPSF_A
• Protein Pathways: Spliceosome
• Gene Summary: This gene encodes subunit 3 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 3 has also been identified as a component of the STAGA (SPT3-TAF(II)31-GCN5L acetylase) transcription coactivator-HAT (histone acetyltransferase) complex, and the TFTC (TATA-binding-protein-free TAF(II)-containing complex). These complexes may function in chromatin modification, transcription, splicing, and DNA repair. [provided by RefSeq, Jul 2008]