SF3B3 (NM_012426) Human Untagged Clone

CAT#: SC323872

SF3B3 (untagged)-Human splicing factor 3b, subunit 3, 130kDa (SF3B3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SF3B3
• Synonyms: RSE1; SAP130; SF3b130; STAF130
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_012426.3 TGTTGGAGTTGGTGGTGGCTTAAGTTTTGAAGGGAGGTAGCATCCGTTGGATATCCACAC
  CATCCTTCTCGCTGCAGGCTTTCTTGGACTCCGTACTGTTGGTGTAACCAAGGCCTGGAG
  GTCTGGGTGGCTCAGGTTTCCTGCAGCCATGTTTCTGTACAACTTAACCTTGCAGAGAGC
  CACTGGCATCAGCTTTGCCATTCATGGAAACTTTTCTGGAACCAAACAACAAGAAATTGT
  TGTTTCCCGTGGGAAGATCTTGGAGCTGCTTCGCCCAGACCCCAACACTGGCAAAGTACA
  TACCCTACTCACTGTGGAAGTATTCGGTGTTATCCGGTCACTCATGGCCTTTAGGCTGAC
  AGGTGGCACCAAAGACTACATTGTAGTTGGCAGTGACTCTGGTCGAATTGTTATTTTGGA
  ATACCAGCCATCTAAGAATATGTTTGAGAAGATTCACCAAGAAACCTTTGGCAAGAGTGG
  ATGCCGTCGCATCGTTCCTGGCCAGTTCTTAGCTGTGGATCCCAAAGGGCGAGCCGTTAT
  GATTAGTGCCATTGAGAAACAGAAATTGGTGTATATTTTGAACAGAGATGCTGCAGCCCG
  ACTTACCATTTCATCTCCCCTGGAAGCCCACAAAGCAAACACTTTAGTGTATCATGTAGT
  TGGAGTAGATGTCGGATTTGAAAATCCAATGTTTGCTTGTCTGGAAATGGATTATGAGGA
  AGCAGACAATGATCCAACAGGGGAAGCAGCAGCTAATACCCAGCAGACACTTACTTTCTA
  TGAGCTAGACCTTGGTTTAAATCATGTGGTCCGAAAATACAGTGAACCTTTGGAGGAACA
  CGGCAACTTCCTTATTACAGTTCCAGGAGGGTCAGATGGTCCAAGTGGAGTACTGATCTG
  CTCTGAAAACTATATTACTTACAAGAACTTTGGTGACCAGCCAGATATCCGCTGTCCAAT
  TCCCAGGAGGCGGAATGACCTGGATGACCCTGAAAGAGGAATGATTTTTGTCTGCTCTGC
  AACCCATAAAACCAAATCGATGTTCTTCTCTTTGGCTCAAACTGAGCAGGGAGATATCTT
  TAAGATCACTTTGGAGACAGATGAAGATATGGTTACTGAGATCCGGCTCAAATATTTTGA
  TACTGTACCCGTTGCTGCTGCCATGTGTGTGCTTAAAACAGGGTTCCTTTTTGTAGCATC
  AGAATTTGGAAACCATTACTTATATCAAATTGCACATCTTGGAGATGATGATGAAGAACC
  TGAGTTTTCATCAGCCATGCCTCTGGAAGAAGGAGACACATTCTTTTTTCAGCCAAGACC
  ACTTAAAAACCTTGTGCTGGTTGATGAGTTGGACAGCCTCTCTCCCATTCTGTTTTGCCA
  GATAGCTGATCTGGCCAATGAAGATACTCCACAGTTGTATGTGGCCTGTGGTAGGGGACC
  CCGATCATCTCTGAGAGTCCTAAGACATGGACTTGAGGTGTCAGAAATGGCTGTTTCTGA
  GCTACCTGGTAACCCCAACGCTGTCTGGACAGTGCGTCGACACATTGAAGATGAGTTTGA
  TGCCTACATTATTGTGTCTTTCGTGAATGCCACCCTAGTGTTGTCCATTGGAGAAACTGT
  AGAAGAAGTGACTGACTCTGGGTTCCTGGGGACCACCCCGACCTTGTCCTGCTCCTTATT
  AGGAGATGATGCCTTGGTGCAGGTCTATCCAGATGGCATTCGGCACATACGAGCAGACAA
  GAGAGTCAATGAGTGGAAGACCCCTGGAAAGAAAACAATTGTGAAGTGTGCAGTGAACCA
  GCGACAAGTGGTGATTGCCCTGACAGGAGGAGAGCTGGTCTATTTCGAGATGGATCCTTC
  AGGACAGCTGAATGAGTACACAGAACGGAAGGAGATGTCAGCAGATGTGGTGTGCATGAG
  TCTGGCCAATGTACCCCCTGGAGAGCAGCGGTCTCGCTTCCTGGCTGTGGGGCTTGTGGA
  CAACACTGTCAGAATCATCTCCCTGGATCCCTCAGACTGTTTGCAACCTCTAAGCATGCA
  GGCTCTCCCAGCCCAGCCTGAGTCCTTGTGTATCGTGGAAATGGGTGGGACTGAGAAGCA
  GGATGAGCTGGGTGAGAGGGGCTCGATTGGCTTCCTATACCTGAATATTGGGCTACAGAA
  CGGTGTGCTGCTGAGGACTGTCTTGGACCCTGTCACTGGGGATTTGTCTGATACTCGCAC
  TCGGTACCTGGGGTCCCGTCCTGTGAAGCTCTTCCGAGTCCGAATGCAAGGCCAGGAGGC
  AGTATTGGCCATGTCAAGCCGCTCATGGTTGAGCTATTCTTACCAATCTCGCTTCCATCT
  CACCCCACTGTCTTACGAGACACTGGAATTTGCATCGGGTTTTGCCTCGGAACAGTGTCC
  CGAGGGCATTGTGGCCATCTCCACCAACACCCTACGGATTTTGGCATTAGAGAAGCTCGG
  TGCTGTCTTCAATCAAGTAGCCTTCCCACTGCAGTACACACCCAGGAAATTTGTCATCCA
  CCCTGAGAGTAACAACCTTATTATCATTGAAACGGACCACAATGCCTACACTGAGGCCAC
  GAAAGCTCAGAGAAAGCAGCAGATGGCAGAGGAAATGGTGGAAGCAGCAGGGGAGGATGA
  GCGGGAGCTGGCCGCAGAGATGGCAGCAGCATTCCTCAATGAAAACCTCCCTGAATCCAT
  CTTTGGAGCTCCCAAGGCTGGCAATGGGCAGTGGGCCTCTGTGATCCGAGTGATGAATCC
  CATTCAAGGGAACACACTGGACCTTGTCCAGCTGGAACAGAATGAGGCAGCTTTTAGTGT
  GGCTGTGTGCAGGTTTTCCAACACTGGTGAAGACTGGTATGTGCTGGTGGGTGTGGCCAA
  GGACCTGATACTAAACCCCCGATCTGTGGCAGGGGGCTTCGTCTATACTTACAAGCTTGT
  GAACAATGGGGAAAAACTGGAGTTTTTGCACAAGACTCCTGTGGAAGAGGTCCCTGCTGC
  TATTGCCCCATTCCAGGGGAGGGTGTTGATTGGTGTGGGGAAGCTGTTGCGTGTCTATGA
  CCTGGGAAAGAAGAAGTTACTCCGAAAATGTGAGAATAAGCATATTGCCAATTATATCTC
  TGGGATCCAGACTATCGGACATAGGGTAATTGTATCTGATGTCCAAGAAAGTTTCATCTG
  GGTTCGCTACAAGCGTAATGAAAACCAGCTTATCATCTTTGCTGATGATACCTACCCCCG
  ATGGGTCACTACAGCCAGCCTCCTGGACTATGACACTGTGGCTGGGGCAGACAAGTTTGG
  CAACATATGTGTGGTGAGGCTCCCACCTAACACCAATGATGAAGTAGATGAGGATCCTAC
  AGGAAACAAAGCCCTGTGGGACCGTGGCTTGCTCAATGGGGCCTCCCAGAAGGCAGAGGT
  GATCATGAACTACCATGTCGGGGAGACGGTGCTGTCCTTGCAGAAGACCACGCTGATCCC
  TGGAGGCTCAGAATCACTTGTCTATACCACCTTGTCTGGAGGAATTGGCATCCTTGTGCC
  ATTCACGTCCCATGAGGACCATGACTTCTTCCAGCATGTGGAAATGCACCTGCGGTCTGA
  ACATCCCCCTCTCTGTGGGCGGGACCACCTCAGCTTTCGCTCCTACTACTTCCCTGTGAA
  GAATGTGATTGATGGAGACCTCTGTGAGCAGTTCAATTCCATGGAACCCAACAAACAAAA
  GAACGTCTCTGAAGAACTGGACCGAACCCCACCCGAAGTGTCCAAGAAACTCGAGGATAT
  CCGGACCCGCTACGCCTTCTGAGCCCTCCTTTCCCGGTGGGGCTTGCCAGAGACTGTGTG
  TTTTGTTTCCCCCACCACCATCACTGCCACCTGGCTTCTGCCATGTGGCAGGAGGGTGAC
  TGGATAATTAAGACTGCATTATGAAAGTCAACAGCTCTTTCCCCTCAGCTCTTCTCCTGG
  AATGACTGGCTTCCCCTCAAATTGGCACTGAGATTTGCTACACTTCTCCCCACCTGGTAC
  ATGATACATGACCCCAGGTTCCAGTGTAGAACCTGAGTCCCCCATTCCCCAAAGCCATCC
  CTGCATTGATATGTCTTGACTCTCCTGTCTACTTTTGCACACACCCTTAATTTTTAATTG
  GTTTTCTTGTAAAAAAAAAAAAAAAAAA
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_012426.3 unedited
  GACACCGCTGTAATACGACTCCCATAGGGCGGCCGGCGACTCGCGGCGCGCCGGTACCGG
  TCCGGAATTCCCGGGATATCGTCGACCCACGCGTCCGTGTTGGAGTTGGTGGTGGCTTAA
  GTTTTGAAGGGAGGTAGCATCCGTTGGATATCCACACCATCCTTCTCGCTGCAGGCTTTC
  TTGGACTCCGTACTGTTGGTGTAACCAAGGCCTGGAGGTCTGGGTGGCTCAGGTTTCCTG
  CAGCCATGTTTCTGTACAACTTAACCTTGCAGAGAGCCACTGGCATCAGCTTTGCCATTC
  ATGGAAACTTTTCTGGAACCAAACAACAAGAAATTGTTGTTTCCCGTGGGAAGATCTTGG
  AGCTGCTTCGCCCAGACCCCAACACTGGCAAAGTACATACCCTACTCACTGTGGAAGTAT
  TCGGTGTTATCCGGTCACTCATGGCCTTTAGGCTGACAGGTGGCACCAAAGACTACATTG
  TAGTTGGCAGTGACTCTGGTCGAATTGTTATTTTGGAATACCAGCCATCTAAGAATATGT
  TTGAGAAGATTCACCAAGAAACCTTTGGCAAGAGTGGATGCCGTCGCATCGTTCCTGGCC
  AGTTCTTAGCTGTGGATCCCAAAGGGCGAGCCGTTATGATTAGTGCCATTGAGAAACAGA
  AATTGGTGTATATTTTGAACAGAGATGCTGCAGCCCGACTTACCATTTCATCTCCCCTGA
  AGCCCACAAAGCAACACTTTAGTGTATCATGTAGTTGGAGTAGATGTCGGATTTGAAAAT
  CCAATGTTTGCTTGTCTGGAAATGGATTATGAGGAGCAGACATGATCCAACAGGGGAGCA
  GCAGCTAATACCCAGCAGAACCTACTTTCTATGACTAGACCTTGTTTAATTCATTGTCCG
  AAATACAGTGACTTTGGAGAAACACGCACTTCTTATACGTTCCAGGAAGGTCAATGGTCC
  AATG
• Restriction Sites: ECoRI-NOT
• ACCN: NM_012426
• ORF Size: 3654 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_012426.3, NP_036558.3
• RefSeq Size: 4357
• RefSeq ORF: 3654
• Locus ID: 23450
• Domains: CPSF_A
• Protein Pathways: Spliceosome
• Gene Summary: This gene encodes subunit 3 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 3 has also been identified as a component of the STAGA (SPT3-TAF(II)31-GCN5L acetylase) transcription coactivator-HAT (histone acetyltransferase) complex, and the TFTC (TATA-binding-protein-free TAF(II)-containing complex). These complexes may function in chromatin modification, transcription, splicing, and DNA repair. [provided by RefSeq, Jul 2008]