VIP (NM_194435) Human Untagged Clone

CAT#: SC126753

VIP (untagged)-Human vasoactive intestinal peptide (VIP), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: VIP
• Synonyms: PHM27
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_194435, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGACACCAGAAATAAGGCCCAGCTCCTTGTGCTCCTGACTCTTCTCAGTGTGCTCTTCTCACAGACTT
  CGGCATGGCCTCTTTACAGGGCACCTTCTGCTCTCAGGTTGGGTGACAGAATACCCTTTGAGGGAGCAAA
  TGAACCTGATCAAGTTTCATTAAAAGAAGACATTGACATGTTGCAAAATGCATTAGCTGAAAATGACACA
  CCCTATTATGATGTATCCAGAAATGCCAGGCATGCTGATGGAGTTTTCACCAGTGACTTCAGTAAACTCT
  TGGGTCAACTTTCTGCCAAAAAGTACCTTGAGTCTCTTATGGGAAAACGTGTTAGTAACATCTCAGAAGA
  CCCTGTACCAGTCAAACGTCACTCAGATGCAGTCTTCACTGACAACTATACCCGCCTTAGAAAACAAATG
  GCTGTAAAGAAATATTTGAACTCAATTCTGAATGGAAAGAGGAGCAGTGAGGGAGAATCTCCCGACTTTC
  CAGAAGAGTTAGAAAAATGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_194435 unedited
  ATACGACTACTATAGGCGGCCGCGAATTCGGCACGAGGCCTCGTGCCGAATTCGGCACGA
  GGGCCAACCCTTAGCCATTGCTAAGGGCAGAGAACTGGTGGAGCCTTTCTCTTACTCCCA
  GGACTTCAGCACCTAAGACAGCTCCAAAACAAACCAGAACAGTCAGCTCCGGGGGAGCAC
  GACTGGGCGAGAGGCACAGAAATGGACACCAGAAATAAGGCCCAGCTCCTTGTGCTCCTG
  ACTCTTCTCAGTGTGCTCTTCTCACAGACTTCGGCATGGCCTCTTTACAGGGCACCTTCT
  GCTCTCAGGTTGGGTGACAGAATACCCTTTGAGGGAGCAAATGAACCTGATCAAGTTTCA
  TTAAAAGAAGACATTGACATGTTGCAAAATGCATTAGCTGAAAATGACACACCCTATTAT
  GATGTATCCAGAAATGCCAGGCATGCTGATGGAGTTTTCACCAGTGACTTCAGTAAACTC
  TTGGGTCAACTTTCTGCCAAAAAGTACCTTGAGTCTCTTATGGGAAAACGTNGTAGCAGT
  AACATCTCAGAAGACCCTGTACCAGTCAAACGTCACTCAGATGCAGTCTTCACTGACAAC
  TATACCCGCCTTAGAAAACANATGGCTGTANAGAAATATTTGCACTCATTTCTGAATGGA
  AAGAGGAGCAGTGAGGGGAGAATCTCCCGACTTTCCAGAAGAGTTAGAAGATGATGAAAA
  CGACCTTTGGAGCCGAGCTGATGACACCTTCCAGTGCACTCTTGAGGGACACGATCGCAC
  ATATTAATTTTGAGTCTACCTAAGTATCAAGAGACAACTTCATATCAAACCAATAAAAAT
  TGGGGTGTGAAGTGTGATGTTTTACCTAAGAATACCTGGAGCTACATGTAATAGTTTGGG
  AGTCTAATTTGGCTTACTATAAAAGCGGCATTCAATGCGGTATCCTTTCACACAAATATT
  TCAGAAAGGAAGGCTGGTATTCATTTGAGAAGTCTGAAGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_194435 unedited
  CGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAATTATGGCAAAAATATTTAT
  TATTGAACAACTGAGTGTTTCATTCACTTCACATTTTACACATACTTAACACAAGCAGAA
  AGGGAAGTTTCAGTGTCAGAAGCAGGGGTCGTTCTCCTTAACTGAAAGACTATCAGGAGA
  GAGCCTTTGGTGATTAGCAATCCAAATTTGAGATTTTTTAAAACACCATTAAAAATAAAG
  GTTGCCTATAAAATATGGAAACACATAATATTTTCTGAAAATCTTTTTGGAAGAATGTCT
  TTTTATCTTCATTGTTACTATCTTAGACATTTTGAGGTTTATTTAACACAGCATTCCATC
  TGCTTAACATTCAAATTTAGACACACTGATTATCTGTTCTACTCTCCTGAATAGTTAAGT
  ACACTCTATAAATAAACACATCAATTTTGCTCTATTACTACTCTTCCAAAAACGTCTCAT
  ATAATTGGAATTAACCTAGCATTTACTTATCATTAAATATATTTTTTTGTTAGAAAGGAT
  ATACAGCATATGAAATTGCAGGCTTTTTATGAGTTAAAGACAAAATTTAGAACTCTCAAA
  TACTATTTACAATGTAAACTTCACAGTTATTACATTAGCTAGAATACATCACAACATTCA
  CAACACAATATTTTTATTTGGTTTGGATATTGAAGTTGTTTTCTTGAATTACTTATGTAG
  AACTCAAAATTTAATTATGTTGCGTATCGTTTTCCTTCAAGAATTCACTGGNGAAGTGTC
  ATCAGCTTTGCTCCAAGGTCTTTTTATCATTTTCTAACTCTTCTGGAAGTCGGGAGATCT
  NCCTCACTGCTCTCTTTCATTCAGAAATGAGTCAATATTNCTTAACGCCATTGTTTCTAA
  GCGGGTATAGTGCAGTGAGACTGCTCTGATGACGTTGACTGTACGGGNTTCTGAGAGTAC
  TGCTACCGTTTCCTAAGAAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_194435
• Insert Size: 1470 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_194435.1, NP_919416.1
• RefSeq Size: 1615 bp
• RefSeq ORF: 510 bp
• Locus ID: 7432
• Cytogenetics: 6q25.2
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Gene Summary: 'The protein encoded by this gene belongs to the glucagon family. It stimulates myocardial contractility, causes vasodilation, increases glycogenolysis, lowers arterial blood pressure and relaxes the smooth muscle of trachea, stomach and gall bladder. The protein also acts as an antimicrobial peptide with antibacterial and antifungal activity. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Nov 2014]'
  Transcript Variant: This variant (2) lacks an alternate in-frame segment, compared to variant 1, resulting in a shorter protein (isoform 2), compared to isoform 1.