PSMC5 (NM_002805) Human Untagged Clone

CAT#: SC126754

PSMC5 (untagged)-Human proteasome (prosome, macropain) 26S subunit, ATPase, 5 (PSMC5), transcript variant 1


  "NM_002805" in other vectors (6)

Reconstitution Protocol

USD 310.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol PSMC5
Synonyms p45; p45/SUG; S8; SUG-1; SUG1; TBP10; TRIP1
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC126754 sequence for NM_002805 edited (data generated by NextGen Sequencing)
ATGGCGCTTGACGGACCAGAGCAGATGGAGCTGGAGGAGGGGAAGGCAGGCAGCGGACTC
CGCCAATATTATCTGTCCAAGATTGAAGAACTCCAGCTGATTGTGAATGATAAGAGCCAA
AACCTCCGGAGGCTGCAGGCACAGAGGAACGAACTAAATGCTAAAGTTCGCCTATTGCGG
GAGGAGCTACAGCTGCTGCAGGAGCAGGGCTCCTATGTGGGGGAAGTAGTCCGGGCCATG
GATAAGAAGAAAGTGTTGGTCAAGGTACATCCTGAAGGTAAATTTGTTGTAGACGTGGAC
AAAAACATTGACATCAATGATGTGACACCCAATTGCCGGGTGGCTCTAAGGAATGACAGC
TACACTCTGCACAAGATCCTGCCCAACAAGGTAGACCCATTAGTGTCACTGATGATGGTG
GAGAAAGTACCAGATTCAACTTATGAGATGATTGGTGGACTGGACAAACAGATCAAGGAG
ATCAAAGAAGTGATCGAGCTGCCTGTTAAGCATCCTGAGCTCTTCGAAGCACTGGGCATT
GCTCAGCCCAAGGGAGTGCTGCTGTATGGACCTCCAGGCACTGGGAAGACACTGTTGGCC
CGGGCTGTGGCTCATCATACGGACTGTACCTTTATTCGTGTCTCTGGCTCTGAATTGGTA
CAGAAATTCATAGGGGAAGGGGCAAGAATGGTGAGGGAGCTGTTTGTCATGGCACGGGAA
CATGCTCCATCTATCATCTTCATGGACGAAATCGACTCCATCGGCTCCTCGCGGCTGGAG
GGGGGTTCTGGAGGGGACAGTGAAGTGCAGCGCACGATGCTGGAGTTGCTCAACCAGCTC
GACGGCTTTGAGGCCACCAAGAACATCAAGGTTATCATGGCTACTAATAGGATTGATATC
CTGGACTCGGCACTGCTTCGCCCAGGGCGCATTGACAGAAAAATTGAATTCCCACCCCCC
AATGAGGAGGCCCGGCTGGACATTTTGAAGATTCATTCTCGGAAGATGAACCTGACCCGG
GGGATCAACCTGAGAAAAATTGCTGAGCTCATGCCAGGAGCATCAGGGGCTGAAGTGAAG
GGCGTGTGCACAGAAGCTGGCATGTATGCCCTGCGAGAACGGCGAGTCCATGTCACTCAG
GAGGACTTTGAGATGGCAGTAGCCAAGGTCATGCAGAAGGACAGTGAGAAAAACATGTCC
ATCAAGAAATTATGGAAGTGA

Clone variation with respect to NM_002805.5
655 c=>t
>OriGene 5' read for NM_002805 unedited
ATTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGAGAGAAGAGGCGC
TTGACGGACCAGAGCAGATGGAGCTGGAGGAGGGGAAGGCAGGCAGCGGACTCCGCCAAT
ATTATCTGTCCAAGATTGAAGAACTCCAGCTGATTGTGAATGATAAGAGCCAAAACCTCC
GGAGGCTGCAGGCACAGAGGAACGAACTAAATGCTAAAGTTCGCCTATTGCGGGAGGAGC
TACAGCTGCTGCAGGAGCAGGGCTCCTATGTGGGGGAAGTAGTCCGGGCCATGGATAAGA
AGAAAGTGTTGGTCAAGGTACATCCTGAAGGTAAATTTGTTGTAGACGTGGACAAAAACA
TTGACATCAATGATGTGACACCCAATTGCCGGGTGGCTCTAAGGAATGACAGCTACACTC
TGCACAAGATCCTGCCCAACAAGGTAGACCCATTAGTGTCACTGATGATGGTGGAGAAAG
TACCAGATTCAACTTATGAGATGATTGGTGGACTGGACAAACAGATCAAGGAGATCAAAG
AAGTGATCGAGCTGCCTGTTAAGCATCCTGAGCTCTTCGAAGCACTGGGCATTGCTCAGC
CCAAGGGAGTGCTGCTGTATGGACCTCCAGGCACTGGGAAGACACTGTTGGCCCGGGCTG
TGGCTCATATACGGACTGTACCTTTATTCGTGTCTCTGGCTCTGAATTGGTACAGAAATT
CTAGGGGAAGGGGCATGAATGGTGAGGGAGCTNGTTGTCATGCACGGGACATGCTCATCT
ATATCTCATGACAAATCACTCATCGCTCTCCGCTGAGGGGGGTCTGAGGGCCANTGATGC
ACGCACATGCTGATGCTCACACTCGACGCTTGAGCACAGACTCAGGTTATGCTCAA
>OriGene 3' read for NM_002805 unedited
CGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGGACTTGGCCCACAGAGCT
TTATTGGAGAGATACACACAAAGGCTGTCCACTCACTTCCATAATTTCTTGATGGACATG
TTTTTCTCACTGTCCTTCTGCATGACCTTGGCTACTGCCATCTCAAAGTCCTCCTGAGTG
ACATGGACTCGCCGTTCTCGCAGGGCATACATGCCAGCTTCTGTGCACACGCCCTTCACT
TCAGCCCCTGATGCTCCTGGCATGAGCTCAGCAATTTTTCTCAGGTTGATCCCCCGGGTC
AGGTTCATCTTCCGAGAATGAATCTTCAAAATGTCCAGCCGGGCCTCCTCATTGGGGGGT
GGGAATTCAATTTTTCTGTCAATGCGCCCTGGGCGAAGCAGTGCCGAGTCCAGGATATCA
ATCCTATTAGTAGCCATGATAACCTTGATGTTCTTGGTGGCCCTCAAAGCCGTCGAGCTG
GTTGAGCAACTCCAGCATCGTGCGCTGCACTTCACTGTCCCCTCCAGAACCCCCCTCCAG
CCGCGAGGAGCCGATGGAGTCGATTTCGCCCATGAAGATGATAGATGGAGCATGTTCCCG
TGCCATGACAAACAAGCTCCTCACCATTCTTTGCCCCTTCCCCTATGAATTTCTGTACCA
ATTCAGAGCCCGAGACACGAATAAATGTACAGTCCGTATGATGAGCCCCAACCCCGGCGC
AACCAGTGTCTTCCCAGTGCCTGAAAGGCCATACAGCAACACTCCCCTGGGCCTGACCCA
AGCCCATGGCTTTAAAAAGCCCCAGAAGCTTAACAGCCAGCTCGACACTTTCTTGACCCC
CCTGACCGGTTGTCCAGTCCCACCAAACTTCCAAAGTTGGAACTGGGCACTTCTCCCCCA
TTATCATGAAACTAAAGGGCCCACCTT
Restriction Sites NotI-NotI     
ACCN NM_002805
Insert Size 1430 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002805.4, NP_002796.4
RefSeq Size 1332 bp
RefSeq ORF 1221 bp
Locus ID 5705
Cytogenetics 17q23.3
Domains AAA, AAA
Protein Families Druggable Genome
Protein Pathways Proteasome
Gene Summary 'The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases which have a chaperone-like activity. In addition to participation in proteasome functions, this subunit may participate in transcriptional regulation since it has been shown to interact with the thyroid hormone receptor and retinoid X receptor-alpha. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]'
Transcript Variant: This variant (1) encodes the longer isoform (1).

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