PSMC5 (NM_002805) Human Untagged Clone

CAT#: SC126754

PSMC5 (untagged)-Human proteasome (prosome, macropain) 26S subunit, ATPase, 5 (PSMC5), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PSMC5
• Synonyms: p45; p45/SUG; S8; SUG-1; SUG1; TBP10; TRIP1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC126754 sequence for NM_002805 edited (data generated by NextGen Sequencing)
  ATGGCGCTTGACGGACCAGAGCAGATGGAGCTGGAGGAGGGGAAGGCAGGCAGCGGACTC
  CGCCAATATTATCTGTCCAAGATTGAAGAACTCCAGCTGATTGTGAATGATAAGAGCCAA
  AACCTCCGGAGGCTGCAGGCACAGAGGAACGAACTAAATGCTAAAGTTCGCCTATTGCGG
  GAGGAGCTACAGCTGCTGCAGGAGCAGGGCTCCTATGTGGGGGAAGTAGTCCGGGCCATG
  GATAAGAAGAAAGTGTTGGTCAAGGTACATCCTGAAGGTAAATTTGTTGTAGACGTGGAC
  AAAAACATTGACATCAATGATGTGACACCCAATTGCCGGGTGGCTCTAAGGAATGACAGC
  TACACTCTGCACAAGATCCTGCCCAACAAGGTAGACCCATTAGTGTCACTGATGATGGTG
  GAGAAAGTACCAGATTCAACTTATGAGATGATTGGTGGACTGGACAAACAGATCAAGGAG
  ATCAAAGAAGTGATCGAGCTGCCTGTTAAGCATCCTGAGCTCTTCGAAGCACTGGGCATT
  GCTCAGCCCAAGGGAGTGCTGCTGTATGGACCTCCAGGCACTGGGAAGACACTGTTGGCC
  CGGGCTGTGGCTCATCATACGGACTGTACCTTTATTCGTGTCTCTGGCTCTGAATTGGTA
  CAGAAATTCATAGGGGAAGGGGCAAGAATGGTGAGGGAGCTGTTTGTCATGGCACGGGAA
  CATGCTCCATCTATCATCTTCATGGACGAAATCGACTCCATCGGCTCCTCGCGGCTGGAG
  GGGGGTTCTGGAGGGGACAGTGAAGTGCAGCGCACGATGCTGGAGTTGCTCAACCAGCTC
  GACGGCTTTGAGGCCACCAAGAACATCAAGGTTATCATGGCTACTAATAGGATTGATATC
  CTGGACTCGGCACTGCTTCGCCCAGGGCGCATTGACAGAAAAATTGAATTCCCACCCCCC
  AATGAGGAGGCCCGGCTGGACATTTTGAAGATTCATTCTCGGAAGATGAACCTGACCCGG
  GGGATCAACCTGAGAAAAATTGCTGAGCTCATGCCAGGAGCATCAGGGGCTGAAGTGAAG
  GGCGTGTGCACAGAAGCTGGCATGTATGCCCTGCGAGAACGGCGAGTCCATGTCACTCAG
  GAGGACTTTGAGATGGCAGTAGCCAAGGTCATGCAGAAGGACAGTGAGAAAAACATGTCC
  ATCAAGAAATTATGGAAGTGA
  
  Clone variation with respect to NM_002805.5
  655 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002805 unedited
  ATTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGAGAGAAGAGGCGC
  TTGACGGACCAGAGCAGATGGAGCTGGAGGAGGGGAAGGCAGGCAGCGGACTCCGCCAAT
  ATTATCTGTCCAAGATTGAAGAACTCCAGCTGATTGTGAATGATAAGAGCCAAAACCTCC
  GGAGGCTGCAGGCACAGAGGAACGAACTAAATGCTAAAGTTCGCCTATTGCGGGAGGAGC
  TACAGCTGCTGCAGGAGCAGGGCTCCTATGTGGGGGAAGTAGTCCGGGCCATGGATAAGA
  AGAAAGTGTTGGTCAAGGTACATCCTGAAGGTAAATTTGTTGTAGACGTGGACAAAAACA
  TTGACATCAATGATGTGACACCCAATTGCCGGGTGGCTCTAAGGAATGACAGCTACACTC
  TGCACAAGATCCTGCCCAACAAGGTAGACCCATTAGTGTCACTGATGATGGTGGAGAAAG
  TACCAGATTCAACTTATGAGATGATTGGTGGACTGGACAAACAGATCAAGGAGATCAAAG
  AAGTGATCGAGCTGCCTGTTAAGCATCCTGAGCTCTTCGAAGCACTGGGCATTGCTCAGC
  CCAAGGGAGTGCTGCTGTATGGACCTCCAGGCACTGGGAAGACACTGTTGGCCCGGGCTG
  TGGCTCATATACGGACTGTACCTTTATTCGTGTCTCTGGCTCTGAATTGGTACAGAAATT
  CTAGGGGAAGGGGCATGAATGGTGAGGGAGCTNGTTGTCATGCACGGGACATGCTCATCT
  ATATCTCATGACAAATCACTCATCGCTCTCCGCTGAGGGGGGTCTGAGGGCCANTGATGC
  ACGCACATGCTGATGCTCACACTCGACGCTTGAGCACAGACTCAGGTTATGCTCAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002805 unedited
  CGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGGACTTGGCCCACAGAGCT
  TTATTGGAGAGATACACACAAAGGCTGTCCACTCACTTCCATAATTTCTTGATGGACATG
  TTTTTCTCACTGTCCTTCTGCATGACCTTGGCTACTGCCATCTCAAAGTCCTCCTGAGTG
  ACATGGACTCGCCGTTCTCGCAGGGCATACATGCCAGCTTCTGTGCACACGCCCTTCACT
  TCAGCCCCTGATGCTCCTGGCATGAGCTCAGCAATTTTTCTCAGGTTGATCCCCCGGGTC
  AGGTTCATCTTCCGAGAATGAATCTTCAAAATGTCCAGCCGGGCCTCCTCATTGGGGGGT
  GGGAATTCAATTTTTCTGTCAATGCGCCCTGGGCGAAGCAGTGCCGAGTCCAGGATATCA
  ATCCTATTAGTAGCCATGATAACCTTGATGTTCTTGGTGGCCCTCAAAGCCGTCGAGCTG
  GTTGAGCAACTCCAGCATCGTGCGCTGCACTTCACTGTCCCCTCCAGAACCCCCCTCCAG
  CCGCGAGGAGCCGATGGAGTCGATTTCGCCCATGAAGATGATAGATGGAGCATGTTCCCG
  TGCCATGACAAACAAGCTCCTCACCATTCTTTGCCCCTTCCCCTATGAATTTCTGTACCA
  ATTCAGAGCCCGAGACACGAATAAATGTACAGTCCGTATGATGAGCCCCAACCCCGGCGC
  AACCAGTGTCTTCCCAGTGCCTGAAAGGCCATACAGCAACACTCCCCTGGGCCTGACCCA
  AGCCCATGGCTTTAAAAAGCCCCAGAAGCTTAACAGCCAGCTCGACACTTTCTTGACCCC
  CCTGACCGGTTGTCCAGTCCCACCAAACTTCCAAAGTTGGAACTGGGCACTTCTCCCCCA
  TTATCATGAAACTAAAGGGCCCACCTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002805
• Insert Size: 1430 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002805.4, NP_002796.4
• RefSeq Size: 1332 bp
• RefSeq ORF: 1221 bp
• Locus ID: 5705
• Cytogenetics: 17q23.3
• Domains: AAA, AAA
• Protein Families: Druggable Genome
• Protein Pathways: Proteasome
• Gene Summary: 'The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases which have a chaperone-like activity. In addition to participation in proteasome functions, this subunit may participate in transcriptional regulation since it has been shown to interact with the thyroid hormone receptor and retinoid X receptor-alpha. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]'
  Transcript Variant: This variant (1) encodes the longer isoform (1).