KRAS (NM_033360) Human Untagged Clone

CAT#: SC126782

KRAS (untagged)-Human v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS), transcript variant a

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KRAS
• Synonyms: C-K-RAS; c-Ki-ras2; CFC2; K-Ras; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KI-RAS; KRAS1; KRAS2; NS; NS3; RALD; RASK2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_033360 edited
  ATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGCGTAGGCAAGAGTGCCTTGACG
  ATACAGCTAATTCAGAATCATTTTGTGGACGAATATGATCCAACAATAGAGGATTCCTAC
  AGGAAGCAAGTAGTAATTGATGGAGAAACCTGTCTCTTGGATATTCTCGACACAGCAGGT
  CAAGAGGAGTACAGTGCAATGAGGGACCAGTACATGAGGACTGGGGAGGGCTTTCTTTGT
  GTATTTGCCATAAATAATACTAAATCATTTGAAGATATTCACCATTATAGAGAACAAATT
  AAAAGAGTTAAGGACTCTGAAGATGTACCTATGGTCCTAGTAGGAAATAAATGTGATTTG
  CCTTCTAGAACAGTAGACACAAAACAGGCTCAGGACTTAGCAAGAAGTTATGGAATTCCT
  TTTATTGAAACATCAGCAAAGACAAGACAGAGAGTGGAGGATGCTTTTTATACATTGGTG
  AGAGAGATCCGACAATACAGATTGAAAAAAATCAGCAAAGAAGAAAAGACTCCTGGCTGT
  GTGAAAATTAAAAAATGCATTATAATGTAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_033360 unedited
  CGGCCGCGAATTCGGCACCAGGCAGCAGCGGCGGCGGCAGTGGCGGCGGCGAAGGTGGCG
  GCGGCTCGGCCAGTACTCCCGGCCCCCGCCATTTCGGACTGGGAGCGAGCGCGGCGCAGG
  CACTGAAGGCGGCGGCGGGGCCAGAGGCTCAGCGGCTCCCAGGTGCGGGAGAGAGGCCTG
  CTGAAAATGACTGAATATTTCTTGTGGTAGTTGGAGCTGGTGGCGTAGGCAAGAGTGCCT
  TGACGATACAGCTAATTCAGAATCATTTTGTGGACGAATATGATCCAACAATAGAGGATT
  CCTACAGGAAGCAAGTAGTAATTGATGGAGAAACCTGTCTCTTGGATATTCTCGACACAG
  CAGGTCAAGAGGAGTACAGTGCAATGAGGGACCAGTACATGAGGACTGGGGAGGGCTTTC
  TTTGTGTATTTGCCATAAATAATACTAAATCATTTGAAGATATTCACCATTATAGAGAAC
  AAATTAAAAGAGTTAAGGACTCTGAAGATGTACCTATGGTCCTAGTAGGAAATAAATGTG
  ATTTGCCTTCTAGAACAGTAGACACAAAACAGGCTCAGGACTTAGCAAGAAGTTATGGAA
  TTCCTTTTATTGAAACATCAGCAAAGACAAGAACAGGGTGTTGATGATGCCTTCTATACA
  TTAGTTCGAGAAATTCGAAACATTAAGAAAGATGAGCANAGATGGTNAAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_033360 unedited
  GCTATGGACCGCGGCACGCATNCTAGTATCGAGTTTTTTTTTTTTTTTTTTAAAAAAACA
  ATATAATCAAGTTTATTCCTTTAAAACAATGAAGTGATTTACATAAAGTAGCTTGATCGA
  AGAGTTTCAGTGGAATCGTAGCAAAACAATTATAGAGCTGGCACAGAGACCAAACCCCTT
  CTTTGCAAAACTAAAATACGCATCGTGTTATCTCTGGGTCGTATACCAAAGGCCTTAGTA
  AGATATTACAGACCACACTAGCACTACCTAAGGACCGGGATTATGTCTCTTGTTTGGGGA
  TACCATATACCCAGTGCCTTGTGCGGTGACTGGCATCTGGTAGGCACTCAATAAATATTT
  GCTGAATAAATGAGTTCTGCAAAACAGGTTTATGAGGCCAAGGTGGGTGAATCACTTGAG
  GTCAGGAGTTCGAGACCAGCCTGGCCAACAGGGTGAAACCCCGTCTCTCCTAAAAATACA
  AAAATTATTTGAGTGTAGTGGCACACGCCTGTAATCCCAGCTACTCAGGAGGCCGAGGCA
  CGAGAATCGCTTGAACCTGCGAGATGGAGGTTGCATGGAGCTGAGATGGCGCCACTGCAT
  TCCACCCTGGGGGACAAGAGCGAGACTTTTGCCCCAAAACAAAAAAGCAAGCTTCATGAT
  TTAAGAATTTTCCCTTTGATGGAACATCCCCGGTAACCAATCAACGGAAGGTTTAACTGG
  TTCCCGATATCAGCCCGACTTTTGATTCCCTAAGAGACCTTTTACCCGTACCTCTGTTAA
  TCTCCCCTCCACCGCCACGGGCCCGCCCCTTCCTCCCATGTGGGCACCCCCCTCGTCCCC
  TGGTATGTTCATATTGTACCGTGTCGCTCGCCGCTTGCGCCTATCCCTCAGCCACGCGCT
  TCGCCCATGTCGCGCCGGTCCGCACGCCGCCCCACGCCCGAGCCCCCCTACCA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_033360
• Insert Size: 3900 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033360.2, NP_203524.1
• RefSeq Size: 5436 bp
• RefSeq ORF: 570 bp
• Locus ID: 3845
• Cytogenetics: 12p12.1
• Domains: ras, RAN, RAS, RHO, RAB
• Protein Families: Druggable Genome
• Protein Pathways: Acute myeloid leukemia, Axon guidance, B cell receptor signaling pathway, Bladder cancer, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Dorso-ventral axis formation, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Gap junction, Glioma, GnRH signaling pathway, Insulin signaling pathway, Long-term depression, Long-term potentiation, MAPK signaling pathway, Melanogenesis, Melanoma, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, T cell receptor signaling pathway, Thyroid cancer, Tight junction, VEGF signaling pathway
• Gene Summary: 'This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (a) is composed of six exons, including exon 4a, which the shorter transcript variant (b) lacks. This rare variant (a) has a cds that terminates in exon 4a and encodes a unique C-terminus, compared to isoform a. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.