DUT (U90223) Human Untagged Clone

CAT#: SC126787

(untagged)-Human deoxyuridine triphosphate nucleotidohydrolase precursor mRNA, nuclear gene encoding mitochondrial protein, complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DUT
• Synonyms: dUTPase
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC126787 sequence for U90223 edited (data generated by NextGen Sequencing)
  ATGACTCCCCTCTGCCCTCGCCCCGCGCTCTGCTACCATTTCCTTACGTCTCTGCTTCGC
  TCAGCGATGCAAAACGCGCGAGGNACGGCAGAGGGCCGAAGCCGCGGTACTCTCCGGGCC
  AGGCCCGCCCCTCGGCCGCNNGCGGCGCAGCACGGGATTCCCCGGCCGCTGTCCAGCGCT
  GGCCGCCTGAGCCAAGGCTGCCGCGGAGCCAGTACAGTCGGGGCCGCTGGCTGGAAGGGC
  GAGCTTCCTAAGGCGGGGGGAAGCCCGGCGCCGGGGCCGGAGACACCCGCCATTTCACCC
  AGTAAGCGGGCCCGGCCTGCGGAGGTGGGCGGCATGCAGCTCCGCTTTGCCCGGCTCTCC
  GAGCACGCCACGGCCCCCACCCGGGGCTCCGCGCGCGCCGCGGGCTACGACCTGTACAGT
  GCCTATGATTACACAATACCACCTATGGAGAAAGCTGTTGTGAAAACGGACATTCAGATA
  GCGCTCCCTTCTGGGTGTTATGGAAGAGTGGCTCCACGGTCAGGCTTGGCTGCAAAACAC
  TTTATTGATGTAGGAGCTGGTGTCATAGATGAAGATTATAGAGGAAATGTTGGTGTTGTA
  CTGTTTAATTTTGGCAAAGAAAAGTTTGAAGTCAAAAAAGGTGATCGAATTGCACAGCTC
  ATTTGCGAACGGATTTTTTATCCAGAAATAGAAGAAGTTCAAGCCTTGGATGACACCGAA
  AGGGGTTCAGGAGGTTTTGGTTCCACTGGAAAGAATTAA
  
  Clone variation with respect to U90223.1
  84 c=>n;140 c=>n;141 g=>n
  5' Read Nucleotide Sequence

  >OriGene 5' read for U90223 unedited
  TGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGAAGCCTGGCGCACG
  TCCGGAGGTGCCGAGGACCCAACCAGCCCAAACTCTGGGGGAAATGACTCCCCTCTGCCC
  TCGCCCCGCGCTCTGCTACCATTTCCTTACGTCTCTGCTTCGCTCAGCGATGCAAAACGC
  GCGAGGCGCACGGCAGAGGGCCGAAGCCGCGGTACTCTCCGGGCCAGGCCCGCCCCTCGG
  CCGCGCCGCGCAGCACGGGATTCCCCGGCCGCTGTCCAGCGCTGGCCGCCTGAGCCAAGG
  CTGCCGCGGAGCCAGTACAGTCGGGGCCGCTGGCTGGAAGGGCGAGCTTCCTAAGGCGGG
  GGGAAGCCCGGCGCCGGGGCCGGAGACACCCGCCATTTCACCCAGTAAGCGGGCCCGGCC
  TGCGGAGGTGGGCGGCATGCAGCTCCGCTTTGCCCGGCTCTCCGAGCACGCCACGGCCCC
  CACCCGGGGCTCCGCGCGCGCCGCGGGCTACGACCTGTACAGTGCCTATGATTACACAAT
  ACCACCTATGGAGAAAGCTGTTGTGAAAACGGACATTCAGATAGCGCTCCCTTCTGGGGT
  GTNTATGGAAGAGTGGCTCCACGGTCAGGCTTGGCTGCAAAACACTNTATTGATGTANGA
  GCTGGTGTCATAGATGAAGATTATGAGGAAATGGNTGGGTGTTGTACTGGTTAATTTTGG
  CANAAANNAGTTTGAGTCANANAGGTGATCGAATTGACAGCTCATTTGCGAACGATTTTT
  TATCCAGAATANAAGAAGTTCAAGCTTGGATACACCNAAGGGTCCAGGGGAGGTTTTTGG
  TTCACTGGGAAGAATTNAAATTATGCCCAGACAGAAAACAGAGAGTCTACCCTTTTTCTA
  AAAAAAAAAAAAAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for U90223 unedited
  GCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAAGAAAAAGTATGACTTCTT
  GTTTTCTGTTCTTGGCATAAATTTTAATTCTTTCCAGTGGAACCAAAACCTCCTGAACCC
  CTTTCGGTGTCATCCAAGGCTTGAACTTCTTCTATTTCTGGATAAAAAATCCGTTCGCAA
  ATGAGCTGTGCAATTCGATCACCTTTTTTGACTTCAAACTTTTCTTTGCCAAAATTAAAC
  AGTACAACACCAACATTTCCTCTATAATCTTCATCTATGACACCAGCTCCTACATCAATA
  AAGTGTTTTGCAGCCAAGCCTGACCGTGGAGCCACTCTTCCATAACACCCAGAAGGGAGC
  GCTATCTGAATGTCCGTTTTCACAACAGCTTTCTCCATAGGTGGTATTGTGTAATCATAG
  GCACTGTACAGGTCGTAGCCCGCGGCGCGCGCGGAGCCCCGGGTGGGGGCCGTGGCGTGC
  TCGGAGAGCCGGGCAAAGCGGAGCTGCATGCCGCCCACCTCCGCAGGCCGGGCCCGCTTA
  CTGGGTGAAATGGCGGGTGTCTCCCGCCCCGGCGCCGGGCTTCCCCCGCCTTAGGAAGCT
  CGCCCTTCCAGCCAGCGGCCCCGACTGTACTGGCTCCGCGGCAGCCTTTGCTTTGGGCGG
  CCAACCGCTGGACACGCGCCCGGGGAAATCCCCGTGCCTGCCCGGCGCCGCTCTAGGGGC
  GTGCCCGTGTCCCGAAAAGCACCCCGGTCTTGCGGCCTTTGTCCCCCCCCCTTCCCGGTT
  TTCCACCCTCTAATAACCTATACACCCCACCGATCGTCACTACCCCCCCCTCTGACTCCT
  TTCGTAGCACACCAGTTCTTCTCCACTTACACATTACTTTATATTCATCCCCCACCCACC
  CTTCTCCCGTTCCTCGTTATACAAATTACCAATTTCTCCCAAATCCGTCCACTTCT
  
• Restriction Sites: NotI-NotI
• ACCN: U90223
• Insert Size: 940 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: U90223.1, AAB94642.1
• RefSeq Size: 960 bp
• RefSeq ORF: 960 bp
• Locus ID: 1854
• Cytogenetics: 15q21.1
• Domains: dUTPase
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Pyrimidine metabolism
• Gene Summary: 'This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19. [provided by RefSeq, Jul 2008]'