CK1 epsilon (CSNK1E) (NM_001894) Human Untagged Clone

CAT#: SC126810

CSNK1E (untagged)-Human casein kinase 1, epsilon (CSNK1E), transcript variant 2


  "NM_001894" in other vectors (6)

Reconstitution Protocol

USD 310.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol CSNK1E
Synonyms CKIe; CKIepsilon; HCKIE
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC126810 sequence for NM_001894 edited (data generated by NextGen Sequencing)
ATGGAGCTACGTGTGGGGAACAAGTACCGCCTGGGACGGAAGATCGGGAGCGGGTCCTTC
GGAGATATCTACCTGGGTGCCAACATCGCCTCTGGTGAGGAAGTCGCCATCAAGCTGGAG
TGTGTGAAGACAAAGCACCCCCAGCTGCACATCGAGAGCAAGTTCTACAAGATGATGCAG
GGTGGCGTGGGGATCCCGTCCATCAAGTGGTGCGGAGCTGAGGGCGACTACAACGTGATG
GTCATGGAGCTGCTGGGGCCTAGCCTCGAGGACCTGTTCAACTTCTGTTCCCGCAAATTC
AGCCTCAAGACGGTGCTGCTCTTGGCCGACCAGATGATCAGCCGCATCGAGTATATCCAC
TCCAAGAACTTCATCCACCGGGACGTCAAGCCCGACAACTTCCTCATGGGGCTGGGGAAG
AAGGGCAACCTGGTCTACATCATCGACTTCGGCCTGGCCAAGAAGTACCGGGACGCCCGC
ACCCACCAGCACATTCCCTACCGGGAAAACAAGAACCTGACCGGCACGGCCCGCTACGCT
TCCATCAACACGCACCTGGGCATTGAGCAAAGCCGTCGAGATGACCTGGAGAGCCTGGGC
TACGTGCTCATGTACTTCAACCTGGGCTCCCTGCCCTGGCAGGGGCTCAAAGCAGCCACC
AAGCGCCAGAAGTATGAACGGATCAGCGAGAAGAAGATGTCAACGCCCATCGAGGTCCTC
TGCAAAGGCTATCCCTCCGAATTCTCAACATACCTCAACTTCTGCCGCTCCCTGCGGTTT
GACGACAAGCCCGACTACTCTTACCTACGTCAGCTCTTCCGCAACCTCTTCCACCGGCAG
GGCTTCTCCTATGACTACGTCTTTGACTGGAACATGCTGAAATTCGGTGCAGCCCGGAAT
CCCGAGGATGTGGACCGGGAGCGGCGAGAACACGAACGCGAGGAGAGGATGGGGCAGCTA
CGGGGGTCCGCGACCCGAGCCCTGCCCCCTGGCCCACCCACGGGGGCCACTGCCAACCGG
CTCCGCAGTGCCGCCGAGCCCGTGGCTTCCACGCCAGCCTCCCGCATCCAGCCGGCTGGC
AATACTTCTCCCAGAGCGATCTCGCGGGTCGACCGGGAGAGGAAGGTGAGTATGAGGCTG
CACAGGGGTGCGCCCGCCAACGTCTCCTCCTCAGACCTCACTGGGCGGCAAGAGGTCTCC
CGGATCCCAGCCTCACAGACAAGTGTGCCATTTGACCATCTCGGGAAGTGA

Clone variation with respect to NM_001894.4
>OriGene 5' read for NM_001894 unedited
NGGTCGCAAATTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCGGGCAGG
AGGCCGGGAGGAGGGAGGCGGCGGCGGCGGCGGCGGCGGCGGCGAGAGCCCAGAGCCAGA
GCCCGGCCGGGGCCGAGCGGAGCGCGGCGGCGGCGGCGGCGGCGGCGGCTGGGCCGGGAG
AGGCTGGCGCGCCGGGCGGCTCCGCGAATCCTCCGGCATCCGCCCCGGCGGGCCGCCCCC
GCCCGCGGCAGCCCCCCGAGCAGTGGCCCGGCATCGGCGCCTTCCCGGCGGGCAAGAGTG
AGCCATGGAGCTACGTGTGGGGAACAAGTACCGCCTGGGACGGAAGATCGGGAGCGGGTC
CTTCGGAGATATCTACCTGGGTGCCAACATCGCCTCTGGTGAGGAAGTCGCCATCAAGCT
GGAGTGTGTGAAGACAAAGCACCCCCAGCTGCACATCGAGAGCAAGTTCTACAAGATGAT
GCAGGGTGGCGTGGGGATCCCGTCCATCAAGTGGTGCGGAGCTGAGGGCGACTACAACGT
GATGGTCATGGAGCTGCTGGGGCCTAGCCTCGAGGACCTGTTCAACTTCTGTTCCCGCAA
ATTCAGCCTCAAGACGGTGCTGCTCTTGGCCGACCAGATGATCAGCCGCATCGAGTATAT
CCACTCCAAGAACTTCATCCACCGGGACGTCAAGCCCCGACACTTTCCTCATGGGGCTGG
GGAAGAANGGGCACCTGGTCTACATCATCGACTTCGGCCTGGCCAAGAAGTACCGGGACG
CCCGCACCACCAGCACATTCCCTACCGGGAAAACAAGAACCCTGACCGGCACGGCCCGCT
ACGCTTCCCATCACACGCACCTGGGCATTGAGCANAGCCGTCCAGATGACCTGGAGAGCC
TNGGCTACGTGCTCATGTACTTCAACCTGGGCTCCCTGCCCTGGCAGGNGCTCAN
>OriGene 3' read for NM_001894 unedited
GTCATTGGGCGATGTGCAACTTCCCAGGNCCAGGNAAAGCACTGGGNGAGGGTCACAGGG
CATGCCACCCGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAG
TTTTTTTTTTTTTTTTTTTTTTAAAGAAAATACAGTNGAAGACACTAAGCAAACACTGGT
CCAATGGGGGCTCTCCTCACTTCCCGAGATGGTCAAATGGCACACTTGTCTGTGAGGCTG
GGATCCGGGAGACCTCTTGCCGCCCAGTGAGGTCTGAGGAGGAGACGTTGGCGGGCGCAC
CCCTGTGCAGCCTCATACTCACCTTCCTCTCCCGGTCGACCCGCGAGATCGCTCTGGGAG
AAGTATTGCCAGCCGGCTGGATGCGGGAGGCTGGCGTGGAAGCCACGGGCTCGGCGGCAC
TGCGGAGCCGGTTGGCAGTGGCCCCCGTGGGTGGGCCAGGGGGCAGGGCTCGGGTCGCGG
ACCCCCGTAGCTGCCCCATCCTCTCCTCGCGTTCGTGTTCTCGCCGCTCCCGGTCCAAAT
CCTCGGGATTCCGGGCTGCACCCTAATTCATCCTGGGGCCCGTCACAGGGAAGGGGCAGG
GGGCTGGCAAGAAAGGGCCCCCATGAATTTCACCATGTTCCAATCAAAAACGTAGTCATA
GAAAAACCCCTGCCGGTGGAATAGGGTGCGGAAGAACTCTACTTACGTAAAAGTATCCGG
CTTTGGTCTCCAAACCCCAGGAGGCGGCAAAAATTGGAGTATGTTTAAAATTCCGGAGGA
AAACCTTTGCAAAAGAACCTCAATGGGCGTTGAAATCTTTCTTCCCCCGCATCCCCTTCA
ATATTTTGGCTCCTTGGCGGCCTGTGTTGCACCCCCCGCCACAGGCAGCGAAGCCCAATT
TTAGAATACATAGAAACCCTGTACCCCACCGCTCTTTGGCACCACACGAAGGCATCTGGC
Restriction Sites NotI-NotI     
ACCN NM_001894
Insert Size 1500 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001894.4, NP_001885.1
RefSeq Size 2670 bp
RefSeq ORF 1251 bp
Locus ID 1454
Cytogenetics 22q13.1
Domains pkinase, TyrKc, S_TKc
Protein Families Druggable Genome, Protein Kinase
Protein Pathways Circadian rhythm - mammal, Hedgehog signaling pathway, Wnt signaling pathway
Gene Summary 'The protein encoded by this gene is a serine/threonine protein kinase and a member of the casein kinase I protein family, whose members have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. The encoded protein is found in the cytoplasm as a monomer and can phosphorylate a variety of proteins, including itself. This protein has been shown to phosphorylate period, a circadian rhythm protein. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Feb 2014]'
Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein.

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