FE65 (APBB1) (NM_145689) Human Untagged Clone

CAT#: SC126863

APBB1 (untagged)-Human amyloid beta (A4) precursor protein-binding, family B, member 1 (Fe65) (APBB1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: APBB1
• Synonyms: FE65; MGC:9072; RIR
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_145689, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTCTGTTCCATCATCACTGAGCCAGTCGGCCATTAATGCCAACAGCCACGGAGGCCCCGCACTGAGCC
  TACCCCTGCCTCTGCACGCTGCCCACAACCAGCTGCTCAACGCCAAGCTGCAGGCCACAGCTGTGGGACC
  CAAGGACCTGCGCAGCGCCATGGGGGAGGGTGGTGGGCCTGAGCCAGGCCCTGCCAATGCCAAGTGGCTA
  AAAGAGGGCCAGAACCAGCTCCGGCGGGCCGCCACGGCCCACCGTGACCAGAATCGCAATGTGACCTTGA
  CCTTGGCGGAGGAGGCCAGCCAGGAGCCTGAGATGGCACCCTTGGGCCCCAAAGGCCTGATACACCTGTA
  CTCTGAGCTGGAGCTCTCAGCTCACAACGCAGCCAACCGAGGCCTACGAGGACCTGGCCTGATCATCAGC
  ACTCAAGAGCAGGGGCCAGATGAGGGAGAGGAGAAGGCGGCCGGGGAGGCCGAGGAGGAGGAGGAGGATG
  ATGATGATGAAGAGGAGGAGGAGGACTTATCTTCTCCCCCAGGGCTGCCTGAGCCCCTGGAGAGTGTGGA
  GGCCCCTCCCAGGCCCCAAGCCCTTACAGATGGCCCCCGGGAACACAGCAAGAGTGCCAGCCTCCTGTTT
  GGCATGCGGAACAGTGCAGCCAGTGATGAGGACTCAAGCTGGGCTACCTTATCCCAGGGCAGCCCCTCCT
  ATGGCTCCCCAGAGGACACAGATTCCTTCTGGAACCCCAACGCCTTCGAGACGGATTCCGACCTGCCGGC
  TGGATGGATGAGGGTCCAGGACACCTCAGGGACCTATTACTGGCACATCCCAACAGGGACCACCCAGTGG
  GAACCCCCCGGCCGGGCCTCCCCCTCACAGGGGAGCAGCCCCCAAGAGGAGTCCCAGCTCACCTGGACAG
  GTTTTGCTCATGGAGAAGGCTTTGAGGATGGAGAATTTTGGAAGGATGAACCCAGTGATGAGGCCCCAAT
  GGAGCTGGGACTGAAGGAACCTGAGGAGGGGACGTTGACCTTCCCAGCTCAGAGCCTCAGCCCAGAGCCG
  TTGCCCCAAGAGGAGGAGAAGCTTCCCCCACGGAATACCAACCCAGGGATCAAGTGTTTCGCCGTGCGCT
  CCCTAGGCTGGGTAGAGATGACCGAGGAGGAGCTGGCCCCTGGACGCAGCAGTGTGGCAGTCAACAATTG
  CATCCGTCAGCTCTCTTACCACAAAAACAACCTGCATGACCCCATGTCTGGGGGCTGGGGGGAAGGAAAG
  GATCTGCTACTGCAGCTGGAGGATGAGACACTAAAGCTAGTGGAGCCACAGAGCCAGGCACTGCTGCACG
  CCCAACCCATCATCAGCATCCGCGTGTGGGGCGTCGGGCGGGACAGTGGAAGGGACTTTGCCTACGTAGC
  TCGTGATAAGCTGACCCAGATGCTCAAGTGCCACGTGTTTCGCTGTGAGGCACCTGCCAAGAACATCGCC
  ACCAGCCTGCATGAGATCTGCTCTAAGATCATGGCCGAACGGCGTAATGCCCGCTGCTTGGTAAATGGAC
  TCTCCCTGGACCACTCTAAACTTGTGGATGTCCCTTTCCAAGTGGAATTCCCAGCGCCTAAGAATGAGTT
  GGTCCAGAAGTTCCAAGTCTATTACCTGGGGAATGTACCTGTTGCTAAACCTGTTGGGGTAGATGTGATT
  AATGGGGCCCTCGAGTCAGTCCTGTCCTCCAGCAGCCGTGAACAATGGACCCCAAGTCATGTCAGTGTGG
  CCCCTGCTACCCTCACCATCTTGCACCAGCAGACAGAGGCAGTGCTGGGAGAGTGTCGGGTGCGTTTCCT
  CTCCTTCCTGGCCGTGGGCAGAGATGTCCACACGTTTGCATTCATCATGGCTGCCGGCCCAGCCTCCTTC
  TGCTGCCACATGTTCTGGTGCGAGCCCAATGCTGCCAGCCTCTCAGAGGCTGTGCAGGCTGCGTGCATGC
  TTCGCTACCAGAAGTGTCTGGATGCCCGTTCCCAGGCCTCCACCTCCTGCCTCCCAGCACCCCCTGCTGA
  GTCTGTGGCACGGCGTGTAGGGTGGACTGTCCGCAGGGGTGTTCAGTCGCTGTGGGGCTCCCTGAAGCCC
  AAACGGCTGGGGGCCCATACCCCATGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_145689 unedited
  CTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTTGTGATGGAGAAGCCGCGGCGGAG
  CCCGAACCCCGCAGCCTGAGCCACCTCCGTCATCTGGGCCCGGGGCCTCACCGCGCAGGA
  GCTGCCAAGGCCATGTCTGTTCCATCATCACTGAGCCAGTCGGCCATTAATGCCAACAGC
  CACGGAGGCCCCGCACTGAGCCTACCCCTGCCTCTGCACGCTGCCCACAACCAGCTGCTC
  AACGCCAAGCTGCAGGCCACAGCTGTGGGACCCAAGGACCTGCGCAGCGCCATGGGGGAG
  GGTGGGGTGGGCCCTNANCCCAAGCCCCTGGCCAATGCCTATGTGGCTACACTGAGGGCC
  CGGACCCAGCTCTCGGCGTGCACGACACGTGACCACCGTGACCAAGAATCACACTTGGGA
  CTCTTGACCCTTGGCGGTAGGATGCCTAACCAGGAATCCTGAAGATGGCACCCCTTGGCT
  ACCCAAAGGCACAGATACACCCTGTAGCTCTGAGCTAGCAGCTCGTCACATAACACTGTA
  TGCCAACCGACGGCCTACTAGGAACCTGTTACCGATCATTACCTTCTGAATATTCCTGAC
  CTGATGATGGACACGAGAAAGCCACCTGAAAAGCCGTTTAGTCCCATGAGAATATTAGCA
  ATATCTGGAGGCCATTTTCTTTGTTACAGCCTCTTAATGCTTAAAACCACGGACGACGCT
  CACAATCAGACACTGGCCAGCCACCTGCCCCCCCGGCAAGAATTATGCAGCCTTGTGCTA
  CGCTGAGGATCCCTGTTTCTACCCCCACATTGGGCTCGGCCACCCTGCAACTTTTACCAC
  CTGCAAAAATTCACCTTTTTTTTTTAAATACAATTGAACTCGCGCCCCCAAAGCTTGGAA
  AATCCTTTCCCCAATAACTTGCTTTTGCCCCCGAAGGAGGCTTACTTCCTTAAACCAAGA
  AGGAACAACCGGTTTGTGTCTTTCCCCTGTTTACCGTGGCGCCCCCAGATTTTCCGCTCT
  TTTCCAGCCTGCCACGCTGGCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_145689 unedited
  CGCGGCACGCTTCTATNATCGAGTTTTTTTTTTTTTTTTTTGCAGAGCAGACAGACTTTA
  TTAGTGATATCAATACAGCAGAGGTGCCCATGGAGCAGGGGGAAGGGACCCATGCCTGGA
  CCAGTCCCTTCCTCCTTCTGCCCCTGCTGGGTCCAGGAGGATGAGGCCTGGCCTGGACCA
  GTTCCTCCTGTTCCACCTGAAACACATGGGGATGGAGAACCAGGGCTGGCCTTGCTTCCT
  GCTCCTCTTGTTACCACTCCAGTGTTATCACTTCCTTGAAGGGATTAGATCTCTCCCTCC
  CCAGCTCCTAGGCAGGGAACCGTAGCTACTGGGGAGGGGCATATTTGGGAGGCCTGAGGC
  CTATGAATAGCCTCTAGACCCCTCCCTGACCCACACCCTTTAGTTCCCTGGGGCCCAACA
  CAAGCAGGTGGAGGGAAGGTGGGGGCTTCTTCATGGGGTATGGGCCCCCAGCCGTTTGGG
  CTTCAGGGAGCCCCACAGCGACTGAACACCCCTGCGGACAGTCCACCCTACACGCCGTGC
  CACAGACTCAGCAGGGGGTGCTGGGAGGCAGGAGGTGGAGGCCTGGGAACGGGCATCCAG
  ACACTTCTGGTAGCGAAGCATGCACGCAGCCTGCACAGCCTCTGAGAGGCTGGCAGCATT
  GGGCTCGCACCAGAACATGTGGCAGCAGAAGGAGGCTGGGCCGGCAGCCATGATGAATGC
  AAACGTGTGGACATCTCTGCCCACGCCAGNAAGAGAGGAAACGCACCCGACACTCTCCCC
  ACACTGCCTCTGTCTGCTGGTGCAAGATGTTGAGGGTAGCAGGGGCCCACTGACATGACT
  TGGGTCCATAGNTCACGGCTGCTGGAGGACAGACTGACTCGAGGGCCCCTTTATCACATT
  TACCCCACAGTTTAGCAACAGTACATCCCCACGTATAGACT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_145689
• Insert Size: 2820 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145689.1, NP_663722.1
• RefSeq Size: 2634 bp
• RefSeq ORF: 2127 bp
• Locus ID: 322
• Cytogenetics: 11p15.4
• Domains: WW, PID
• Protein Families: Transcription Factors
• Protein Pathways: Alzheimer's disease
• Gene Summary: 'The protein encoded by this gene is a member of the Fe65 protein family. It is an adaptor protein localized in the nucleus. It interacts with the Alzheimer's disease amyloid precursor protein (APP), transcription factor CP2/LSF/LBP1 and the low-density lipoprotein receptor-related protein. APP functions as a cytosolic anchoring site that can prevent the gene product's nuclear translocation. This encoded protein could play an important role in the pathogenesis of Alzheimer's disease. It is thought to regulate transcription. Also it is observed to block cell cycle progression by downregulating thymidylate synthase expression. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Mar 2012]'
  Transcript Variant: This variant (2) differs in the 5' UTR and lacks an alternate, in-frame exon in the coding region compared to variant 1. This results in a protein that maintains the reading frame but is a shorter isoform (b, also known as delta E9), compared to isoform a. This encoded isoform (b) is widely expressed in all non-neuronal cells but is not expressed in differentiated neurons.