ACSL3 (NM_203372) Human Untagged Clone

CAT#: SC126917

ACSL3 (untagged)-Human acyl-CoA synthetase long-chain family member 3 (ACSL3), transcript variant 2


  "NM_203372" in other vectors (6)

Reconstitution Protocol

USD 310.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol ACSL3
Synonyms ACS3; FACL3; LACS 3; LACS3; PRO2194
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC126917 sequence for NM_203372 edited (data generated by NextGen Sequencing)
ATGAATAACCACGTGTCTTCAAAACCATCTACCATGAAGCTAAAACATACCATCAACCCT
ATTCTTTTATATTTTATACATTTTCTAATATCACTTTATACTATTTTAACATACATTCCG
TTTTATTTTTTCTCCGAGTCAAGACAAGAAAAATCAAACCGAATTAAAGCAAAGCCTGTA
AATTCAAAACCTGATTCTGCATACAGATCTGTTAATAGTTTGGATGGTTTGGCTTCAGTA
TTATACCCTGGATGTGATACTTTAGATAAAGTTTTTACATATGCAAAAAACAAATTTAAG
AACAAAAGACTCTTGGGAACACGTGAAGTTTTAAATGAGGAAGATGAAGTACAACCAAAT
GGAAAAATTTTTAAAAAGGTTATTCTTGGACAGTATAATTGGCTTTCCTATGAAGATGTC
TTTGTTCGAGCCTTTAATTTTGGAAATGGATTACAGATGTTGGGTCAGAAACCAAAGACC
AACATCGCCATCTTCTGTGAGACCAGGGCCGAGTGGATGATAGCTGCACAGGCGTGTTTT
ATGTATAATTTTCAGCTTGTTACATTATATGCCACTCTAGGAGGTCCAGCCATTGTTCAT
GCATTAAATGAAACAGAGGTGACCAACATCATTACTAGTAAAGAACTCTTACAAACAAAG
TTGAAGGATATAGTTTCTTTGGTCCCACGCCTGCGGCACATCATCACTGTTGATGGAAAG
CCACCGACCTGGTCCGAGTTCCCCAAGGGCATCATTGTGCATACCATGGCTGCAGTGGAG
GCCCTGGGAGCCAAGGCCAGCATGGAAAACCAACCTCATAGCAAACCATTGCCCTCAGAT
ATTGCAGTAATCATGTACACAAGTGGATCCACAGGACTTCCAAAGGGAGTCATGATCTCA
CATAGTAACATTATTGCTGGTATAACTGGGATGGCAGAAAGGATTCCAGAACTAGGAGAG
GAAGATGTCTACATTGGATATTTGCCTCTGGCCCATGTTCTAGAATTAAGTGCTGAGCTT
GTCTGTCTTTCTCACGGATGCCGCATTGGTTACTCTTCACCACAGACTTTAGCAGATCAG
TCTTCAAAAATTAAAAAAGGAAGCAAAGGGGATACATCCATGTTGAAACCAACACTGATG
GCAGCAGTTCCGGAAATCATGGATCGGATCTACAAAAATGTCATGAATAAAGTCAGTGAA
ATGAGTAGTTTTCAACGTAATCTGTTTATTCTGGCCTATAATTACAAAATGGAACAGATT
TCAAAAGGACGTAATACTCCACTGTGCGACAGCTTTGTTTTCCGGAAAGTTCGAAGCTTG
CTAGGGGGAAATATTCGTCTCCTGTTGTGTGGTGGCGCTCCACTTTCTGCAACCACGCAG
CGATTCATGAACATCTGTTTCTGCTGTCCTGTTGGTCAGGGATACGGGCTCACTGAATCT
GCTGGGGCTGGAACAATTTCCGAAGTGTGGGACTACAATACTGGCAGAGTGGGAGCACCA
TTAGTTTGCTGTGAAATCAAATTAAAAAACTGGGAGGAAGGTGGATACTTTAATACTGAT
AAGCCACACCCCAGGGGTGAAATTCTTATTGGGGGCCAAAGTGTGACAATGGGGTACTAC
AAAAATGAAGCAAAAACAAAAGCTGATTTCTTTGAAGATGAAAATGGACAAAGGTGGCTC
TGTACTGGGGATATTGGAGAGTTTGAACCCGATGGATGCTTAAAGATTATTGATCGTAAA
AAGGACCTTGTAAAACTACAGGCAGGGGAATATGTTTCTCTTGGGAAAGTAGAGGCAGCT
TTGAAGAATCTTCCACTAGTAGATAACATTTGTGCATATGCAAACAGTTATCATTCTTAT
GTCATTGGATTTGTTGTGCCAAATCAAAAGGAACTAACTGAACTAGCTCGAAAGAAAGGA
CTTAAAGGGACTTGGGAGGAGCTGTGTAACAGTTGTGAAATGGAAAATGAGGTACTTAAA
GTGCTTTCCGAAGCTGCTATTTCAGCAAGTCTGGAAAAGTTTGAAATTCCAGTAAAAATT
CGTTTGAGTCCTGAACCGTGGACCCCTGAAACTGGTCTGGTGACAGATGCCTTCAAGCTG
AAACGCAAAGAGCTTAAAACACATTACCAGGCGGACATTGAGCGAATGTATGGAAGAAAA
TAA

Clone variation with respect to NM_203372.1
>OriGene 5' read for NM_203372 unedited
ACTTAGGGCGGCCGCGAATTCGGCACGAGGAACAGCTGTAACATTTGCCACCCTCAGAAG
CTGCTGGTCCTGTGTCACACCACCTTAGCCTCTTGATCGAGGAAGATTCTCGCTGAAGTC
TGTTAATTCTACTTTTTGAGTACTTATGAATAACCACGTGTCTTCAAAACCATCTACCAT
GAAGCTAAAACATACCATCAACCCTATTCTTTTATATTTTATACATTTTCTAATATCACT
TTATACTATTTTAACATACATTCCGTTTTATTTTTTCTCCGAGTCAAGACAAGAAAAATC
AAACCGAATTAAAGCAAAGCCTGTAAATTCAAAACCTGATTCTGCATACAGATCTGTTAA
TAGTTTGGATGGTTTGGCTTCAGTATTATACCCTGGATGTGATACTTTAGATAAAGTTTT
TACATATGCAAAAAACAAATTTAAGAACAAAAGACTCTTGGGAACACGTGAAGTTTTAAA
TGAGGAAGATGAAGTACAACCAAATGGAAAAATTTTTAAAAAGGTTATTCTTGGACAGTA
TAATTGGCTNTCCTATGAAGATGTCTTTGTTCGAGCCTTTAATTTGGAAAGGATACAGAT
GNTGGGTCAGAACCAAAGACAACATCGNCATCTTCTGTGAACCAGGNCCGAGTGATGATA
GCTGCACAGGCGTGTNTATGTATATTTTCAGCTTGTACATATATGCCATCTANGAGGTCA
GNCATTGTCATGCATAATGAACAGAGTGACAACTCATACTATAAAGACCTACAACAAGTG
AGGATTAGTTTTGGGCCCACGCTGCGAAAAATACTGTGTGGAAGCACGACTGGCCGGATC
CAAGGCTATGGGCTACTGGTTGAGGGAGCTGGGCCAAGCCTTGAAAACAACTAAAAACCT
GCCTAATTGGGATCTGCCAGGGGCCCGACTCAGGGGGGGGCCAAACCTTGGGGTACGGGG
GAAGACA
>OriGene 3' read for NM_203372 unedited
GCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTTAAAACATAAGTATGGGTAT
ATTTATTTCTCTCAAATGCATACAAGACAATAATTACACAGCAACAAATCTTTTGTTCAA
CAATGATTTGATTCATAAGCATTTGAAATTTACATAATTTCATATCAATACCCTTGTATT
TTTAAATACAGTAAGTAAAAAAGCCCCCAAATAACCAATTCTTATATTTCCTATTTATCC
CTCTATACATCCAAACTTTTAAAAAGTTACAAACTGAACATTATACAGAACATATAAATC
ATGTTTAAAAACTTGAGGTTTTAAAATCACTGCTTCCCCAATATGATTCAGAAAAATTCT
CATACTGACAAGTACAGTCATAGGTGGTAAAGTAAGTTGGTGGCGGGGAAAATGAAAGAA
GAGACAGACACAAGTTTGCTGTCTTAATGTTTTACTAATCCTGTCAGTTAAAAATGGTGA
CGTCATGAGAAGTAATAGTTTAATATGAGGAATGGAGTTTGCCTTGCAGCTTGAGACATG
CATTTCAAGTATTTTCCTATTTGATCTGAGCTCACTGTAGCAAACTGATGCCAGAAGAGA
ATAATTATTTTCTTCCATACATTCGCTCAATGTCCGCCTGGTAATGTGTTTTAAGCTCTT
TGCGTTTCAGCTTGAAGGCATCTGTCACCAGACCAGTTTCAGGGGTCCACGTTTCAGGAC
TCAAACGAATTTTTACTGGAATTTAAACTTTTCCAGACTTGCTGAAATAGCAGCTTCGGA
AAGCACTTTAAGTACCTCATTTTNCATTTCACAACTGNTACACAGCTCCTCCCCAGTCCC
TTTAAGTCCTTTCTTTCCAGCTAGNTCAAGTAGTTCCTTTGATTTTGCCCCACAAATCCA
TGACATTAGAATGATACTGTTTGCCTATGCCAAATGTTATCTACTATGGAAGATTCTTAA
AGCTGCTTTACTTTCCAGAAAAAATTTCNCCCTGCCGTAGTTTAAAGGGCCTTTTAGAAC
AAATTTTTAAACTCATGGGA
Restriction Sites NotI-NotI     
ACCN NM_203372
Insert Size 2800 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_203372.1, NP_976251.1
RefSeq Size 4262 bp
RefSeq ORF 2163 bp
Locus ID 2181
Cytogenetics 2q36.1
Protein Families Transmembrane
Protein Pathways Adipocytokine signaling pathway, Fatty acid metabolism, Metabolic pathways, PPAR signaling pathway
Gene Summary 'The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme is highly expressed in brain, and preferentially utilizes myristate, arachidonate, and eicosapentaenoate as substrates. The amino acid sequence of this isozyme is 92% identical to that of rat homolog. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]'
Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein.

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